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The map label for this gene is gcvH

Identifier: 108799811

GI number: 108799811

Start: 3010375

End: 3010770

Strand: Reverse

Name: gcvH

Synonym: Mmcs_2845

Alternate gene names: 108799811

Gene position: 3010770-3010375 (Counterclockwise)

Preceding gene: 108799812

Following gene: 108799810

Centisome position: 52.77

GC content: 66.41

Gene sequence:

>396_bases
GTGAGCGAGATCCCAGCCGAGCTGTACTACACCGACGAACACGAGTGGGTGCTCCGTACGGGTGACGACACGCTGCGCGT
CGGCATCACCGACTATGCGCAGGCGGCGCTCGGTGACGTCGTGTTCGTACAGCTGCCCGACGTCGGCGCCGAGCTGACCT
CGGGGGAGTCGTTCGGCGAGGTGGAGTCGACCAAGTCGGTATCCGATCTGTATGCCCCCGTCAGCGCGAAAGTGCTTGCC
GTCAACGGAAATCTGGAGGCCAGCCCCGATCTGGTCAACTCCGATCCGTACGGCGAGGGCTGGCTGGTCGACCTGCAGCT
CGACGCCGACGACATGGAGGCGGCGCTGGGCGGTCTGCTGGACGCGGACGGCTACCGTGGCGTGGTGACCGAGTGA

Upstream 100 bases:

>100_bases
CGTGACCGCCTTGCGGCAACCGAAAGACCGCCAATACGCTCAGCCAGTCAAATAGCGTGCCGGCCGCCCCGGCCGCGCGC
GTCGTACGCAGAGGAGACCC

Downstream 100 bases:

>100_bases
GTTGTTGTTAGGGTGCTGGGCGACGGTTCGACCATCTTCGACCGAGTGAAATCGACGAGGGGCGGATCCGGCGGTGGTGG
ACACAATGGCGTCCACTGCG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MSEIPAELYYTDEHEWVLRTGDDTLRVGITDYAQAALGDVVFVQLPDVGAELTSGESFGEVESTKSVSDLYAPVSAKVLA
VNGNLEASPDLVNSDPYGEGWLVDLQLDADDMEAALGGLLDADGYRGVVTE

Sequences:

>Translated_131_residues
MSEIPAELYYTDEHEWVLRTGDDTLRVGITDYAQAALGDVVFVQLPDVGAELTSGESFGEVESTKSVSDLYAPVSAKVLA
VNGNLEASPDLVNSDPYGEGWLVDLQLDADDMEAALGGLLDADGYRGVVTE
>Mature_130_residues
SEIPAELYYTDEHEWVLRTGDDTLRVGITDYAQAALGDVVFVQLPDVGAELTSGESFGEVESTKSVSDLYAPVSAKVLAV
NGNLEASPDLVNSDPYGEGWLVDLQLDADDMEAALGGLLDADGYRGVVTE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=99, Percent_Identity=44.4444444444444, Blast_Score=93, Evalue=5e-20,
Organism=Homo sapiens, GI89057342, Length=99, Percent_Identity=43.4343434343434, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=49.6124031007752, Blast_Score=126, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17551294, Length=122, Percent_Identity=42.6229508196721, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17507493, Length=109, Percent_Identity=49.5412844036697, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=124, Percent_Identity=43.5483870967742, Blast_Score=112, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17865652, Length=130, Percent_Identity=42.3076923076923, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_MYCSJ (A3Q0H8)

Other databases:

- EMBL:   CP000580
- RefSeq:   YP_001071146.1
- ProteinModelPortal:   A3Q0H8
- SMR:   A3Q0H8
- STRING:   A3Q0H8
- EnsemblBacteria:   EBMYCT00000049868
- GeneID:   4878589
- GenomeReviews:   CP000580_GR
- KEGG:   mjl:Mjls_2876
- eggNOG:   COG0509
- GeneTree:   EBGT00050000015839
- HOGENOM:   HBG693789
- OMA:   TPKELRY
- PhylomeDB:   A3Q0H8
- ProtClustDB:   PRK01202
- BioCyc:   MSP164757:MJLS_2876-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13957; Mature: 13826

Theoretical pI: Translated: 3.49; Mature: 3.49

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIPAELYYTDEHEWVLRTGDDTLRVGITDYAQAALGDVVFVQLPDVGAELTSGESFGE
CCCCCCEEEEECCCCEEEEECCCEEEEECHHHHHHHHCCEEEEEECCCCCCCCCCCCCCC
VESTKSVSDLYAPVSAKVLAVNGNLEASPDLVNSDPYGEGWLVDLQLDADDMEAALGGLL
CCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHCCCC
DADGYRGVVTE
CCCCCCCCCCC
>Mature Secondary Structure 
SEIPAELYYTDEHEWVLRTGDDTLRVGITDYAQAALGDVVFVQLPDVGAELTSGESFGE
CCCCCEEEEECCCCEEEEECCCEEEEECHHHHHHHHCCEEEEEECCCCCCCCCCCCCCC
VESTKSVSDLYAPVSAKVLAVNGNLEASPDLVNSDPYGEGWLVDLQLDADDMEAALGGLL
CCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHCCCC
DADGYRGVVTE
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA