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The map label for this gene is splB [H]

Identifier: 108799632

GI number: 108799632

Start: 2814918

End: 2816030

Strand: Reverse

Name: splB [H]

Synonym: Mmcs_2665

Alternate gene names: 108799632

Gene position: 2816030-2814918 (Counterclockwise)

Preceding gene: 108799634

Following gene: 108799625

Centisome position: 49.36

GC content: 65.41

Gene sequence:

>1113_bases
ATGACGTTCGATCCGGGCAGCGGCGACTCTGTGCACCGGGCTCCGGTCGACCTGGCCGATCGGCTTCTCGACATCAAGCG
GATCTACCACGAACCCGGCATCGAGCGGTTCCCGCGGGCCCGCGCCGTACTCGAGCGCTACCCCGACGCCGAGCGGGTCG
AGGTGCTGTCCCATCAGGCGATCCCGGGTCTGTACGGCAACGAGGGCAACGTCGAGGACTGGGTGCGCAACAAGCGCGAG
GTCCTCGTCATCGGTGAGAAGAAGAGCCTCAGCGCCCGACGCAACGAACGCTCCAGCGACTGGATCGCACCGTCGACGGC
CAACGGCTGCGCGATGGCGTGCTCCTACTGCTACGTCCCCCGACGCAAGGGTTATGCGAATCCGATCACGGTGTTCGCCA
ACATCGAGAAGATCACCGGCTACCTGGAACGGCACGCCGCGCGTCAGGGCGCCAAACCCGAACCGAACCAGTGCGACCCG
ATCGACTGGGTGTACGACATCGGTGAGAACAGCGACTGCTCGGCGGACGCGCTGGTGTCCGACAACGTCCGCGACCTCGT
CGACCTGTTCGGGCGGATTCCCAACGCGAAGGCCAGTTTCGCGACGAAGCTGGTCAACCGTGACCTGTTGACGTACGAGC
CGCGCGGGGGGACGCGGGTGCGCTTCTCGCTGATGCCGGCCGAGACGTCACGCCTGGTCGACGTGCGGACCTCGAAGATC
TCCGACCGGATCGCGGCGATCGACGATTTCGTCGAGGCGGGTTACGAGGTGCACCTGAACTTCAGCCCGGTGATCGTGCA
CGAGAACTGGATCGCCGACTGGGCCGACCTGCTCGAGCAGATCGCCGACCAGACCAACGACCGCACCAGACGACAGTTGG
CGGCGGAGGTCATCTTCTTGACCCACAACGAGGGGCTGCACGACGTCAACCTCGGCTGGCATCCGAAGGGCGAGGAGCTG
CTGTGGCGGCCCGATCTCCAGCAGCTCAAACGCAGTCAGAGCGGTCAGTGGAACGTCCGCTACAAGAGCCCCTGGAAGGG
AAAGTGGGTGCAGCAGCTCACCGACCTGGTCGCCGACAGACTGCCGGACTGCCGTATCCGCTACGCGTTCTGA

Upstream 100 bases:

>100_bases
CCGACGGGATCACACCGGCCACCGCGGGGTCGCGCCGGAGGTTGATCCACCTCACTCCCGGTACCGGAACAGCGGCTGTC
ACACACACGTTGATACGGCC

Downstream 100 bases:

>100_bases
GCCGTCGCGTCAGCGGCCTTTCCACTGCGGTTTGCGCTTGTCGCGCCACGCCTGTATCCCTTCGACGACATCGTCGGTGG
CGGCGGCGACCTTGCGCATG

Product: radical SAM family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTFDPGSGDSVHRAPVDLADRLLDIKRIYHEPGIERFPRARAVLERYPDAERVEVLSHQAIPGLYGNEGNVEDWVRNKRE
VLVIGEKKSLSARRNERSSDWIAPSTANGCAMACSYCYVPRRKGYANPITVFANIEKITGYLERHAARQGAKPEPNQCDP
IDWVYDIGENSDCSADALVSDNVRDLVDLFGRIPNAKASFATKLVNRDLLTYEPRGGTRVRFSLMPAETSRLVDVRTSKI
SDRIAAIDDFVEAGYEVHLNFSPVIVHENWIADWADLLEQIADQTNDRTRRQLAAEVIFLTHNEGLHDVNLGWHPKGEEL
LWRPDLQQLKRSQSGQWNVRYKSPWKGKWVQQLTDLVADRLPDCRIRYAF

Sequences:

>Translated_370_residues
MTFDPGSGDSVHRAPVDLADRLLDIKRIYHEPGIERFPRARAVLERYPDAERVEVLSHQAIPGLYGNEGNVEDWVRNKRE
VLVIGEKKSLSARRNERSSDWIAPSTANGCAMACSYCYVPRRKGYANPITVFANIEKITGYLERHAARQGAKPEPNQCDP
IDWVYDIGENSDCSADALVSDNVRDLVDLFGRIPNAKASFATKLVNRDLLTYEPRGGTRVRFSLMPAETSRLVDVRTSKI
SDRIAAIDDFVEAGYEVHLNFSPVIVHENWIADWADLLEQIADQTNDRTRRQLAAEVIFLTHNEGLHDVNLGWHPKGEEL
LWRPDLQQLKRSQSGQWNVRYKSPWKGKWVQQLTDLVADRLPDCRIRYAF
>Mature_369_residues
TFDPGSGDSVHRAPVDLADRLLDIKRIYHEPGIERFPRARAVLERYPDAERVEVLSHQAIPGLYGNEGNVEDWVRNKREV
LVIGEKKSLSARRNERSSDWIAPSTANGCAMACSYCYVPRRKGYANPITVFANIEKITGYLERHAARQGAKPEPNQCDPI
DWVYDIGENSDCSADALVSDNVRDLVDLFGRIPNAKASFATKLVNRDLLTYEPRGGTRVRFSLMPAETSRLVDVRTSKIS
DRIAAIDDFVEAGYEVHLNFSPVIVHENWIADWADLLEQIADQTNDRTRRQLAAEVIFLTHNEGLHDVNLGWHPKGEELL
WRPDLQQLKRSQSGQWNVRYKSPWKGKWVQQLTDLVADRLPDCRIRYAF

Specific function: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6- dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines [H]

COG id: COG1533

COG function: function code L; DNA repair photolyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. SPL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR004594 [H]

Pfam domain/function: NA

EC number: =4.1.99.14 [H]

Molecular weight: Translated: 42194; Mature: 42063

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFDPGSGDSVHRAPVDLADRLLDIKRIYHEPGIERFPRARAVLERYPDAERVEVLSHQA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHC
IPGLYGNEGNVEDWVRNKREVLVIGEKKSLSARRNERSSDWIAPSTANGCAMACSYCYVP
CCCCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCC
RRKGYANPITVFANIEKITGYLERHAARQGAKPEPNQCDPIDWVYDIGENSDCSADALVS
CCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEECCCCCCCCCCHHHC
DNVRDLVDLFGRIPNAKASFATKLVNRDLLTYEPRGGTRVRFSLMPAETSRLVDVRTSKI
CCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCCHHHHHHHHHHH
SDRIAAIDDFVEAGYEVHLNFSPVIVHENWIADWADLLEQIADQTNDRTRRQLAAEVIFL
HHHHHHHHHHHHCCEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEE
THNEGLHDVNLGWHPKGEELLWRPDLQQLKRSQSGQWNVRYKSPWKGKWVQQLTDLVADR
ECCCCCCCCCCCCCCCCCCEEECCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHC
LPDCRIRYAF
CCCCEEEECC
>Mature Secondary Structure 
TFDPGSGDSVHRAPVDLADRLLDIKRIYHEPGIERFPRARAVLERYPDAERVEVLSHQA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHC
IPGLYGNEGNVEDWVRNKREVLVIGEKKSLSARRNERSSDWIAPSTANGCAMACSYCYVP
CCCCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCC
RRKGYANPITVFANIEKITGYLERHAARQGAKPEPNQCDPIDWVYDIGENSDCSADALVS
CCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEECCCCCCCCCCHHHC
DNVRDLVDLFGRIPNAKASFATKLVNRDLLTYEPRGGTRVRFSLMPAETSRLVDVRTSKI
CCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCCHHHHHHHHHHH
SDRIAAIDDFVEAGYEVHLNFSPVIVHENWIADWADLLEQIADQTNDRTRRQLAAEVIFL
HHHHHHHHHHHHCCEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEE
THNEGLHDVNLGWHPKGEELLWRPDLQQLKRSQSGQWNVRYKSPWKGKWVQQLTDLVADR
ECCCCCCCCCCCCCCCCCCEEECCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHC
LPDCRIRYAF
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8449881; 9384377; 9353924 [H]