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The map label for this gene is mbtM

Identifier: 108798691

GI number: 108798691

Start: 1860363

End: 1861928

Strand: Reverse

Name: mbtM

Synonym: Mmcs_1723

Alternate gene names: 108798691

Gene position: 1861928-1860363 (Counterclockwise)

Preceding gene: 108798692

Following gene: 108798690

Centisome position: 32.63

GC content: 70.18

Gene sequence:

>1566_bases
TTGGCGTCGGCGCTGGCCGATGCGATGACGTCCAGCGGCCGCGACCTGGTCGTCCTCGACCACGGGGGCTGGCGCCGTCA
TCCCTGGGCGGAAGTGCACCAGCGCGCCGAGAACATCGCCGCCCGCATCACCGAGGACGCCGCCACCGTCGTCGTCCTCG
TCGGCGAACCCACCGTCGAGTTCATCGCGGCGATCCCCGGCACGCTGTATGCCGGTGCCGCGCTGTCGATCCTGCCCGGG
CCCGTCCGCGGCGCCGACCTCGGACAGTGGGCGCGCAACACGATGCAGCGGTGCGCGTCGATCGGGGTTCGCCTGGTCTT
CAGCCACGGCGAGCATCTCGACGCGCTGCGCGCCACCCCGGCCTCGATCCCCACGTGTGACGTGACGGAGACGGGGCACG
CTCGGCGGGACTCGTCGTTCACACCGCCGGATCACGGTGAGTTCGCGGTGCTGCAGGGCACCGCGGGATCGACCGGCATC
CCGCGCACGGCCCAGTTGTCACCCGAGGCGGTGCTGGCGAATCTGCGCGGCCTTGTCGCGTGCATCGACATCACGCCCGC
CGACACCGGCTGCTCGTGGCTGCCGCTCTATCACGACATGGGGCTGGCGTTCCTGCTCACCAATGCGCTCGGCGGGGCCG
AACTCTGGCAGGCGCCGACGACCGCGTTCTCGGCGTCACCGTTTAGCTGGCTGCAATGGATGACCGAGAGCACGGCCACG
CTGACCGCGGCGCCCAATATGGCCTACGGCCTGATCGGCAAGTACGGTAGCCGGCTCACCGACTTCGACCTCGGCGCTCT
GCGGTTCGCACTCAACGGCGGTGAACCCGTCGACTGCCAGGGTTACCGCAGGTTCGCCACCGAGATGGCGCGTTTCGGCC
TCGACCCGTCCGCCCTCGTGCCCGCCTATGGTCTGGCCGAATCCTCCTGTGCGGTGACCGTTCCGATCCTCGGCTCCGGC
CTACAGGTCGACGAGGTCCAGGTGAGTTCGGACGACGGCCGGTTCATCCGCACACATGCCGTCGTCGGTGAGCCGATCCC
CGGCATGCAGGTGCGGATCGCACCCACGGACGGCTCCGGCGAGCAGGTCGACGGCCGGGACTTCGGAGAGATCGAGGTCC
GCGGGACGTCGCTGATGTCGGGATACCTCGGCGAACCCCCGCTGGAGGCCGGATCATGGTTCCCCACCGGCGATCTCGGC
TATTTCGTCGACAGCGGGCTCGTTGTGTGCGGTCGCGCGAAGGAGCTGATCACCGTCGCCGGGCGCAACATCTTCCCGGC
CGAGGTGGAACGCGTCGCCGCCCAGGTGCAGGGCGTCCGGGAAGGGGCGGTCGTCGCCGTCGGCACCGACGAAAGCGCCG
TGCGGCCGGGGCTGGTCATCGCCGCCGAGTTCCGCGGGACCGACGAACCGGCCGCCCGCACTGAACTCGTGCAGCGGGTG
GCGTCGGAATGCGGCGTGGTGCCGTCGAACGTGGTGTTCCTCGAACCCGGCGCCCTCCCCCGCACGTCGTCGGGCAAGCT
GCGCCGGCTGGAGGTCAAACGAAGCATGGAGGACGTGAACCGGTGA

Upstream 100 bases:

>100_bases
CGCTGACCGAGGAAGACCTGCTCAGCTGTGACACGGTCGGCGATCTCGAAGCGGCGATCCAGGCGAAGGTGCCGAGTTCC
CCGAGCGGCCAGTGAACCCG

Downstream 100 bases:

>100_bases
CACTCGCCGCCCACACCACCGAGGACTACGAAGAACTTCTCGGCCGGGTATTCGATGATCAGGTGAAGGCCTGGACGGCC
GAAGCCGAAGCGTCCGAACG

Product: long-chain-fatty-acid--[acyl-carrier-protein] ligase

Products: NA

Alternate protein names: Fatty acyl-[acyl-carrier-protein] synthetase; Fatty acyl-ACP synthetase; Mycobactin synthetase protein M

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MASALADAMTSSGRDLVVLDHGGWRRHPWAEVHQRAENIAARITEDAATVVVLVGEPTVEFIAAIPGTLYAGAALSILPG
PVRGADLGQWARNTMQRCASIGVRLVFSHGEHLDALRATPASIPTCDVTETGHARRDSSFTPPDHGEFAVLQGTAGSTGI
PRTAQLSPEAVLANLRGLVACIDITPADTGCSWLPLYHDMGLAFLLTNALGGAELWQAPTTAFSASPFSWLQWMTESTAT
LTAAPNMAYGLIGKYGSRLTDFDLGALRFALNGGEPVDCQGYRRFATEMARFGLDPSALVPAYGLAESSCAVTVPILGSG
LQVDEVQVSSDDGRFIRTHAVVGEPIPGMQVRIAPTDGSGEQVDGRDFGEIEVRGTSLMSGYLGEPPLEAGSWFPTGDLG
YFVDSGLVVCGRAKELITVAGRNIFPAEVERVAAQVQGVREGAVVAVGTDESAVRPGLVIAAEFRGTDEPAARTELVQRV
ASECGVVPSNVVFLEPGALPRTSSGKLRRLEVKRSMEDVNR

Sequences:

>Translated_521_residues
MASALADAMTSSGRDLVVLDHGGWRRHPWAEVHQRAENIAARITEDAATVVVLVGEPTVEFIAAIPGTLYAGAALSILPG
PVRGADLGQWARNTMQRCASIGVRLVFSHGEHLDALRATPASIPTCDVTETGHARRDSSFTPPDHGEFAVLQGTAGSTGI
PRTAQLSPEAVLANLRGLVACIDITPADTGCSWLPLYHDMGLAFLLTNALGGAELWQAPTTAFSASPFSWLQWMTESTAT
LTAAPNMAYGLIGKYGSRLTDFDLGALRFALNGGEPVDCQGYRRFATEMARFGLDPSALVPAYGLAESSCAVTVPILGSG
LQVDEVQVSSDDGRFIRTHAVVGEPIPGMQVRIAPTDGSGEQVDGRDFGEIEVRGTSLMSGYLGEPPLEAGSWFPTGDLG
YFVDSGLVVCGRAKELITVAGRNIFPAEVERVAAQVQGVREGAVVAVGTDESAVRPGLVIAAEFRGTDEPAARTELVQRV
ASECGVVPSNVVFLEPGALPRTSSGKLRRLEVKRSMEDVNR
>Mature_520_residues
ASALADAMTSSGRDLVVLDHGGWRRHPWAEVHQRAENIAARITEDAATVVVLVGEPTVEFIAAIPGTLYAGAALSILPGP
VRGADLGQWARNTMQRCASIGVRLVFSHGEHLDALRATPASIPTCDVTETGHARRDSSFTPPDHGEFAVLQGTAGSTGIP
RTAQLSPEAVLANLRGLVACIDITPADTGCSWLPLYHDMGLAFLLTNALGGAELWQAPTTAFSASPFSWLQWMTESTATL
TAAPNMAYGLIGKYGSRLTDFDLGALRFALNGGEPVDCQGYRRFATEMARFGLDPSALVPAYGLAESSCAVTVPILGSGL
QVDEVQVSSDDGRFIRTHAVVGEPIPGMQVRIAPTDGSGEQVDGRDFGEIEVRGTSLMSGYLGEPPLEAGSWFPTGDLGY
FVDSGLVVCGRAKELITVAGRNIFPAEVERVAAQVQGVREGAVVAVGTDESAVRPGLVIAAEFRGTDEPAARTELVQRVA
SECGVVPSNVVFLEPGALPRTSSGKLRRLEVKRSMEDVNR

Specific function: Activates lipidic moieties required for mycobactin biosynthesis. Converts medium- to long-chain aliphatic fatty acids into acyl adenylate, which is further transferred on to the phosphopantetheine arm of the carrier protein MbtL

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family

Homologues:

Organism=Homo sapiens, GI156151445, Length=385, Percent_Identity=25.7142857142857, Blast_Score=80, Evalue=7e-15,
Organism=Escherichia coli, GI145693145, Length=387, Percent_Identity=26.6149870801034, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788107, Length=377, Percent_Identity=25.4641909814324, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17558820, Length=371, Percent_Identity=23.989218328841, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=386, Percent_Identity=23.8341968911917, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=395, Percent_Identity=25.5696202531646, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=378, Percent_Identity=26.4550264550265, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71994703, Length=425, Percent_Identity=24.7058823529412, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994694, Length=425, Percent_Identity=24.7058823529412, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=425, Percent_Identity=24.7058823529412, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=400, Percent_Identity=23, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18859661, Length=414, Percent_Identity=23.6714975845411, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21355181, Length=547, Percent_Identity=23.217550274223, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356947, Length=150, Percent_Identity=32, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24581924, Length=376, Percent_Identity=25.2659574468085, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20130357, Length=370, Percent_Identity=25.4054054054054, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24653035, Length=178, Percent_Identity=29.2134831460674, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MBTM_MYCSS (Q1BBA2)

Other databases:

- EMBL:   CP000384
- RefSeq:   YP_638888.1
- ProteinModelPortal:   Q1BBA2
- SMR:   Q1BBA2
- STRING:   Q1BBA2
- EnsemblBacteria:   EBMYCT00000061585
- GeneID:   4110557
- GenomeReviews:   CP000384_GR
- KEGG:   mmc:Mmcs_1723
- eggNOG:   COG0318
- GeneTree:   EBGT00050000015137
- HOGENOM:   HBG547964
- OMA:   HRNILAG
- ProtClustDB:   PRK05851
- BioCyc:   MSP164756:MMCS_1723-MONOMER
- InterPro:   IPR020845
- InterPro:   IPR000873

Pfam domain/function: PF00501 AMP-binding

EC number: =6.2.1.20

Molecular weight: Translated: 55021; Mature: 54890

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASALADAMTSSGRDLVVLDHGGWRRHPWAEVHQRAENIAARITEDAATVVVLVGEPTVE
CCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEECCCEEEEEEECCCHHH
FIAAIPGTLYAGAALSILPGPVRGADLGQWARNTMQRCASIGVRLVFSHGEHLDALRATP
HHHHCCCHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHCCC
ASIPTCDVTETGHARRDSSFTPPDHGEFAVLQGTAGSTGIPRTAQLSPEAVLANLRGLVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCEEE
CIDITPADTGCSWLPLYHDMGLAFLLTNALGGAELWQAPTTAFSASPFSWLQWMTESTAT
EEEECCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCEE
LTAAPNMAYGLIGKYGSRLTDFDLGALRFALNGGEPVDCQGYRRFATEMARFGLDPSALV
EEECCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHC
PAYGLAESSCAVTVPILGSGLQVDEVQVSSDDGRFIRTHAVVGEPIPGMQVRIAPTDGSG
CCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCEEEEEECCCCC
EQVDGRDFGEIEVRGTSLMSGYLGEPPLEAGSWFPTGDLGYFVDSGLVVCGRAKELITVA
CCCCCCCCCEEEEECHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCEEEECCCCEEHEEC
GRNIFPAEVERVAAQVQGVREGAVVAVGTDESAVRPGLVIAAEFRGTDEPAARTELVQRV
CCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHH
ASECGVVPSNVVFLEPGALPRTSSGKLRRLEVKRSMEDVNR
HHHCCCCCCCEEEECCCCCCCCCCCCEEEHHHHHHHHHHCC
>Mature Secondary Structure 
ASALADAMTSSGRDLVVLDHGGWRRHPWAEVHQRAENIAARITEDAATVVVLVGEPTVE
CHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEECCCEEEEEEECCCHHH
FIAAIPGTLYAGAALSILPGPVRGADLGQWARNTMQRCASIGVRLVFSHGEHLDALRATP
HHHHCCCHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHCCC
ASIPTCDVTETGHARRDSSFTPPDHGEFAVLQGTAGSTGIPRTAQLSPEAVLANLRGLVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCEEE
CIDITPADTGCSWLPLYHDMGLAFLLTNALGGAELWQAPTTAFSASPFSWLQWMTESTAT
EEEECCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCEE
LTAAPNMAYGLIGKYGSRLTDFDLGALRFALNGGEPVDCQGYRRFATEMARFGLDPSALV
EEECCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHC
PAYGLAESSCAVTVPILGSGLQVDEVQVSSDDGRFIRTHAVVGEPIPGMQVRIAPTDGSG
CCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCEEEEEECCCCC
EQVDGRDFGEIEVRGTSLMSGYLGEPPLEAGSWFPTGDLGYFVDSGLVVCGRAKELITVA
CCCCCCCCCEEEEECHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCEEEECCCCEEHEEC
GRNIFPAEVERVAAQVQGVREGAVVAVGTDESAVRPGLVIAAEFRGTDEPAARTELVQRV
CCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHH
ASECGVVPSNVVFLEPGALPRTSSGKLRRLEVKRSMEDVNR
HHHCCCCCCCEEEECCCCCCCCCCCCEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA