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The map label for this gene is punA [H]

Identifier: 108798215

GI number: 108798215

Start: 1346965

End: 1347816

Strand: Reverse

Name: punA [H]

Synonym: Mmcs_1244

Alternate gene names: 108798215

Gene position: 1347816-1346965 (Counterclockwise)

Preceding gene: 108798218

Following gene: 108798213

Centisome position: 23.62

GC content: 73.24

Gene sequence:

>852_bases
TTGACGCAGTATCTCACTGCACGAATCGCGCGGCTTCGACAGCTAAGCTCGCACACCGTGACCAGCCCAGCGGAGGTGGC
CCGGCAGGCCGCCGCGGTGATCCGCGACCGCACCGGAGCCGACGAACACGACGTCGCGGTCGTGCTCGGATCGGGCTGGG
CGCCCGCGGCCGACGCCCTCGGCACCGCCACCGCGTCCGTGCCGATGGCCGAGTTGCCCGGGTTCACGCCGCCGACGGCC
GCGGGCCACGGCGGGGTCGCGCTGTCCGTTCCGATCGGCGGGCACCGCGTGCTGGTCCTCGTCGGCCGCATCCACGCCTA
CGAGGGCCACGATCTGCGCCACGTCGTGCACCCGGTGCGGGCGGCGTGCGCGGCGGGCGTGCGCACCGTCGTGCTGACCA
ACGCCGCCGGCGGGCTCCGCGAGGACTACACCGTGGGTCAGCCGGTGGTGATCAGCGACCACCTCAACCTCACCGCCCGC
TCCCCGCTGGTGGGGGCGCAATTCGTCGATCTGGTCGACGCGTACGCACCGCGGCTGCGAACACTGGCCAGGACGATCGA
CCCGACCCTGGCCGAAGGCGTCTACGCCGGACTGCCGGGGCCGCACTACGAGACCCCGGCCGAGATCCGGATGCTGCGCA
CGCTCGGCGCGGATCTGGTCGGCATGTCGACAGTGCACGAGACGATCGCCGCGCGCGCGGCGGGCGCCGAGGTGCTGGCG
ATCTCGCTGGTGACCAATCTGGCCGCCGGCATGACCGGGCAGCCGCTCTCGCACCAGGAGGTGCTGGAGGCGGGCCGTCA
GTCCGCGACGCGGATGGGTGCTCTGCTGGCCGGCGTCGTCGCATCGCTCTGA

Upstream 100 bases:

>100_bases
AGTTCCGGCTCGGCGTGGATGGCGTGTGACAACTCGACGAGGTCTCCGCGGCGGCGGTGGACGGCGTCTTCGACGCTCGT
CGACGCGGTGGCGGAGGGCA

Downstream 100 bases:

>100_bases
TCGCCGGCGTGACGGCTCAGCCGCCGGCTCGCCCACACGACGAAGCGGGCCAGCAGCGGTGAGGCGAACAGCATGAGCAG
CACCCGCAAGACCTGCGCGG

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: PNP; Inosine phosphorylase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTQYLTARIARLRQLSSHTVTSPAEVARQAAAVIRDRTGADEHDVAVVLGSGWAPAADALGTATASVPMAELPGFTPPTA
AGHGGVALSVPIGGHRVLVLVGRIHAYEGHDLRHVVHPVRAACAAGVRTVVLTNAAGGLREDYTVGQPVVISDHLNLTAR
SPLVGAQFVDLVDAYAPRLRTLARTIDPTLAEGVYAGLPGPHYETPAEIRMLRTLGADLVGMSTVHETIAARAAGAEVLA
ISLVTNLAAGMTGQPLSHQEVLEAGRQSATRMGALLAGVVASL

Sequences:

>Translated_283_residues
MTQYLTARIARLRQLSSHTVTSPAEVARQAAAVIRDRTGADEHDVAVVLGSGWAPAADALGTATASVPMAELPGFTPPTA
AGHGGVALSVPIGGHRVLVLVGRIHAYEGHDLRHVVHPVRAACAAGVRTVVLTNAAGGLREDYTVGQPVVISDHLNLTAR
SPLVGAQFVDLVDAYAPRLRTLARTIDPTLAEGVYAGLPGPHYETPAEIRMLRTLGADLVGMSTVHETIAARAAGAEVLA
ISLVTNLAAGMTGQPLSHQEVLEAGRQSATRMGALLAGVVASL
>Mature_282_residues
TQYLTARIARLRQLSSHTVTSPAEVARQAAAVIRDRTGADEHDVAVVLGSGWAPAADALGTATASVPMAELPGFTPPTAA
GHGGVALSVPIGGHRVLVLVGRIHAYEGHDLRHVVHPVRAACAAGVRTVVLTNAAGGLREDYTVGQPVVISDHLNLTARS
PLVGAQFVDLVDAYAPRLRTLARTIDPTLAEGVYAGLPGPHYETPAEIRMLRTLGADLVGMSTVHETIAARAAGAEVLAI
SLVTNLAAGMTGQPLSHQEVLEAGRQSATRMGALLAGVVASL

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=257, Percent_Identity=37.3540856031128, Blast_Score=148, Evalue=5e-36,
Organism=Escherichia coli, GI1788746, Length=250, Percent_Identity=35.6, Blast_Score=145, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17541190, Length=272, Percent_Identity=37.8676470588235, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323238, Length=306, Percent_Identity=33.9869281045752, Blast_Score=142, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24656093, Length=281, Percent_Identity=34.1637010676157, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24656090, Length=272, Percent_Identity=34.1911764705882, Blast_Score=131, Evalue=4e-31,
Organism=Drosophila melanogaster, GI45552887, Length=270, Percent_Identity=34.0740740740741, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45552885, Length=275, Percent_Identity=33.4545454545455, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24762376, Length=222, Percent_Identity=28.8288288288288, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011269
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29196; Mature: 29065

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQYLTARIARLRQLSSHTVTSPAEVARQAAAVIRDRTGADEHDVAVVLGSGWAPAADAL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
GTATASVPMAELPGFTPPTAAGHGGVALSVPIGGHRVLVLVGRIHAYEGHDLRHVVHPVR
HHHHCCCCHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEHHHCCCCCCHHHHHHHHH
AACAAGVRTVVLTNAAGGLREDYTVGQPVVISDHLNLTARSPLVGAQFVDLVDAYAPRLR
HHHHHCCEEEEEECCCCCCCCCCCCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHH
TLARTIDPTLAEGVYAGLPGPHYETPAEIRMLRTLGADLVGMSTVHETIAARAAGAEVLA
HHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHH
ISLVTNLAAGMTGQPLSHQEVLEAGRQSATRMGALLAGVVASL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQYLTARIARLRQLSSHTVTSPAEVARQAAAVIRDRTGADEHDVAVVLGSGWAPAADAL
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
GTATASVPMAELPGFTPPTAAGHGGVALSVPIGGHRVLVLVGRIHAYEGHDLRHVVHPVR
HHHHCCCCHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEHHHCCCCCCHHHHHHHHH
AACAAGVRTVVLTNAAGGLREDYTVGQPVVISDHLNLTARSPLVGAQFVDLVDAYAPRLR
HHHHHCCEEEEEECCCCCCCCCCCCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHH
TLARTIDPTLAEGVYAGLPGPHYETPAEIRMLRTLGADLVGMSTVHETIAARAAGAEVLA
HHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHH
ISLVTNLAAGMTGQPLSHQEVLEAGRQSATRMGALLAGVVASL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]