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The map label for this gene is truA [H]

Identifier: 108798087

GI number: 108798087

Start: 1212707

End: 1213606

Strand: Direct

Name: truA [H]

Synonym: Mmcs_1115

Alternate gene names: 108798087

Gene position: 1212707-1213606 (Clockwise)

Preceding gene: 108798086

Following gene: 108798091

Centisome position: 21.26

GC content: 71.78

Gene sequence:

>900_bases
ATGACGAACGAGCCCGCCATCGACACCGATGGCGGGCTTGTTCATTTGTCCTCCTCGCGTGCGGTGACTCGTCTTCGGCT
CGACGTCAGTTACGACGGCACCGACTTCGCGGGGTGGGCGCCGCAGACCGGTCAGCGCACCGTCGCGGGGCTGATCGACG
AGGCGCTGACGACGGTGTTCCGCACGCCGGTGACCGTGCGGGCGGCCGGGCGCACCGACGCGGGCGTGCACGCCACCGGT
CAGGTCGCCCATGTCGACGTACCGGATGACGCGCTGCCCCACGCGTACCCGCGCAGCGCCCGGCCCGACGAACCCGAGTT
CCTGCCGCTGGTCCGTCGGCTCGGGCGGTTCCTTCCCGCCGACGTCCGGATCCGCGACATCATCAGGGCGCCCACGGGGT
TCGACGCCCGCTTCTCCGCGCTGCGACGCCACTACACCTACCGGCTCGGCACCGCGCCGTACGGGGTGGAGCCGCAGTCG
GCGCGATTCGTCACGCCGTGGCCGCGGCCGCTCGACGTCGAGGCGATGAACGACGCCGGCTCGCAGTTGGTCGGGCTGCG
TGACTTCGCGGCGTTCTGCCGGCATCGCGAGGGGGCCACCACGATTCGCGATCTGCAGCGGCTGGAGTGGACGCGCGACG
GCGACCTGGTCACCGCCTATGTCAGCGCGGACGCGTTCTGCTGGTCGATGGTCCGCTCGCTCGTCGGCGCGCTGATCGCG
GTGGGGGAGCACCGCCGCGAACCGGAGTGGTGCGCCGGATTGTTGAGCGCGACACGGCGGTCCAGCGAGTTCGCCGCCGC
ACCGCCCCAGGGACTCACGCTCGTCGGCGTGGACTATCCGCCGGACGACGAACTGGCGGCACGCACGCTGGTCACCCGCG
ACCTGCGCGTCATCGACTAG

Upstream 100 bases:

>100_bases
GCTCTGCCGGAAGCCGAGGACACCACGGCTTCGGAGGCGTCGCGGTCCACCGAGACCGACGATCCCACCCAGGATTCGGA
CGCTGACAAGTCGTAACAGC

Downstream 100 bases:

>100_bases
GGGCGATTTCGGCGTGCCCAGTCGCGCGTAGCACTACCCAGCACGCCGAAATCGCAGCGTCAGATCCGGCCGGCGATGAA
ATCCGCGGCCTGGTTGACCA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTNEPAIDTDGGLVHLSSSRAVTRLRLDVSYDGTDFAGWAPQTGQRTVAGLIDEALTTVFRTPVTVRAAGRTDAGVHATG
QVAHVDVPDDALPHAYPRSARPDEPEFLPLVRRLGRFLPADVRIRDIIRAPTGFDARFSALRRHYTYRLGTAPYGVEPQS
ARFVTPWPRPLDVEAMNDAGSQLVGLRDFAAFCRHREGATTIRDLQRLEWTRDGDLVTAYVSADAFCWSMVRSLVGALIA
VGEHRREPEWCAGLLSATRRSSEFAAAPPQGLTLVGVDYPPDDELAARTLVTRDLRVID

Sequences:

>Translated_299_residues
MTNEPAIDTDGGLVHLSSSRAVTRLRLDVSYDGTDFAGWAPQTGQRTVAGLIDEALTTVFRTPVTVRAAGRTDAGVHATG
QVAHVDVPDDALPHAYPRSARPDEPEFLPLVRRLGRFLPADVRIRDIIRAPTGFDARFSALRRHYTYRLGTAPYGVEPQS
ARFVTPWPRPLDVEAMNDAGSQLVGLRDFAAFCRHREGATTIRDLQRLEWTRDGDLVTAYVSADAFCWSMVRSLVGALIA
VGEHRREPEWCAGLLSATRRSSEFAAAPPQGLTLVGVDYPPDDELAARTLVTRDLRVID
>Mature_298_residues
TNEPAIDTDGGLVHLSSSRAVTRLRLDVSYDGTDFAGWAPQTGQRTVAGLIDEALTTVFRTPVTVRAAGRTDAGVHATGQ
VAHVDVPDDALPHAYPRSARPDEPEFLPLVRRLGRFLPADVRIRDIIRAPTGFDARFSALRRHYTYRLGTAPYGVEPQSA
RFVTPWPRPLDVEAMNDAGSQLVGLRDFAAFCRHREGATTIRDLQRLEWTRDGDLVTAYVSADAFCWSMVRSLVGALIAV
GEHRREPEWCAGLLSATRRSSEFAAAPPQGLTLVGVDYPPDDELAARTLVTRDLRVID

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=286, Percent_Identity=30.0699300699301, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI199559785, Length=267, Percent_Identity=26.5917602996255, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1788657, Length=264, Percent_Identity=36.7424242424242, Blast_Score=148, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6321112, Length=247, Percent_Identity=31.9838056680162, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 32857; Mature: 32726

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNEPAIDTDGGLVHLSSSRAVTRLRLDVSYDGTDFAGWAPQTGQRTVAGLIDEALTTVF
CCCCCCCCCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RTPVTVRAAGRTDAGVHATGQVAHVDVPDDALPHAYPRSARPDEPEFLPLVRRLGRFLPA
CCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
DVRIRDIIRAPTGFDARFSALRRHYTYRLGTAPYGVEPQSARFVTPWPRPLDVEAMNDAG
CCHHHHHHHCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCEEECCCCCCCCHHCCCCCC
SQLVGLRDFAAFCRHREGATTIRDLQRLEWTRDGDLVTAYVSADAFCWSMVRSLVGALIA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHH
VGEHRREPEWCAGLLSATRRSSEFAAAPPQGLTLVGVDYPPDDELAARTLVTRDLRVID
HHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEECC
>Mature Secondary Structure 
TNEPAIDTDGGLVHLSSSRAVTRLRLDVSYDGTDFAGWAPQTGQRTVAGLIDEALTTVF
CCCCCCCCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RTPVTVRAAGRTDAGVHATGQVAHVDVPDDALPHAYPRSARPDEPEFLPLVRRLGRFLPA
CCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
DVRIRDIIRAPTGFDARFSALRRHYTYRLGTAPYGVEPQSARFVTPWPRPLDVEAMNDAG
CCHHHHHHHCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCEEECCCCCCCCHHCCCCCC
SQLVGLRDFAAFCRHREGATTIRDLQRLEWTRDGDLVTAYVSADAFCWSMVRSLVGALIA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHH
VGEHRREPEWCAGLLSATRRSSEFAAAPPQGLTLVGVDYPPDDELAARTLVTRDLRVID
HHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]