Click here to switch to the map view.

The map label for this gene is metZ [H]

Identifier: 108797506

GI number: 108797506

Start: 582166

End: 583404

Strand: Direct

Name: metZ [H]

Synonym: Mmcs_0526

Alternate gene names: 108797506

Gene position: 582166-583404 (Clockwise)

Preceding gene: 108797505

Following gene: 108797507

Centisome position: 10.2

GC content: 67.72

Gene sequence:

>1239_bases
ATGAGCTCAGAGGTGCCCTCGCGCACGCAAGGAGCGGACAAGCCCTCCGTCCGCCGGCCCGCCGAACTGCCCGAGGGGGT
CGGTCAGGCCACCATCGGGGTGCGCGGCGGTCTGCTGCGGTCCGGTTTCGAGGAGACCGCCGAGGCTCTGTACCTCACGT
CGGGCTATGTGTACGCGTCGGCGGCCGAGGCGGAGAAGGCGTTCACCGGAGAGATCGACCGGTACGTCTATTCGCGGTAC
GGCAACCCGACGATCTCGATGTTCGAGGAGCGGCTGCGGCTGATCGAGGGGGCGCCGGCCTGTTTCGCGACGGCGACCGG
CATGGCCGCGGTGTTCACGTCGCTGGGCGCGCTGCTGGGCGCGGGGGACCGGTTGGTTGCCGCGCGCTCACTGTTCGGTT
CGTGTTTCGTGGTCTGCAGCGAGATCCTGCCGCGTTGGGGTGTGGAGACGGTGTTCGTCGACGGCGACGACCTCTCCCAG
TGGGAGGAGGCCCTCTCGGTGCCCACGCAGGCGGTGTTCTTCGAGACACCGTCGAACCCCATGCAGTCGCTGGTCGACAT
CGCCGCGGTGACGGAGCTCGCGCATGCGGCGGGCGCAAAAGTGGTGTTGGACAACGTCTTCGCCACGCCGCTGCTGCAGC
AGGGTTTCCCGCTGGGGGTCGACGTGGTGGTCTACTCCGGCACCAAGCACATCGACGGTCAGGGCCGGGTGCTCGGAGGC
GCGATCCTCGGTTCGCAGGAGTACATCGACGGCCCGGTGCAGAAGTTGATGCGTCACACCGGCCCGGCGATCAGCGCGTT
CAACGCGTGGACGCTGTTGAAGGGTTTGGAGACGCTGGCGGTACGGGTCGACTACTCCAACCGGTCGGCGGAGCGGATCG
CGGAGTTCCTCGAGGGGCACTCCTCCGTGAGTTGGGTCAAGTACCCGTTCCTGGAGTCCCATCCGCAGTACGACCTGGCC
AAACGGCAGATGCGCGGCGGCGGCACCGTCGTCACGTTCGAACTCGCCGGCGGTAAGGAACGGGCCTTCGAGGTGCTCGA
CAAACTGCAGATCATCGACATCTCGAACAACCTCGGCGACGCCAAGTCGTTGATCACCCATCCCGCCACCACGACCCACC
GGGCGATGGGTCCGGAGGGTCGCGCGGCGATCGGTCTGGGCGACGGTGTGGTGCGGGTGTCGGTCGGGCTCGAGGACACC
GACGACCTGATCCGCGATCTCGACCGGGCGCTGGGCTGA

Upstream 100 bases:

>100_bases
CGTCCTACAACATCCTCGACGGGTTCGAAGGTGACCTCGACGAGCAGAAGCACCGTGGTCGCACCGGTTGGAAGGCCGTG
GGACTGCCCTGGAAGCAGTC

Downstream 100 bases:

>100_bases
GTGTCCCGGCGCTCGGAGGCGAAGAAGGCGCGGCGGCGTAAACGCCAGGCGGCGCAGAACGTGGCCTGGGTGCCCGAGGC
GGTGGTGGAACAGTTGTCGA

Product: O-succinylhomoserine sulfhydrylase

Products: cystathionine; succinate

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSSEVPSRTQGADKPSVRRPAELPEGVGQATIGVRGGLLRSGFEETAEALYLTSGYVYASAAEAEKAFTGEIDRYVYSRY
GNPTISMFEERLRLIEGAPACFATATGMAAVFTSLGALLGAGDRLVAARSLFGSCFVVCSEILPRWGVETVFVDGDDLSQ
WEEALSVPTQAVFFETPSNPMQSLVDIAAVTELAHAAGAKVVLDNVFATPLLQQGFPLGVDVVVYSGTKHIDGQGRVLGG
AILGSQEYIDGPVQKLMRHTGPAISAFNAWTLLKGLETLAVRVDYSNRSAERIAEFLEGHSSVSWVKYPFLESHPQYDLA
KRQMRGGGTVVTFELAGGKERAFEVLDKLQIIDISNNLGDAKSLITHPATTTHRAMGPEGRAAIGLGDGVVRVSVGLEDT
DDLIRDLDRALG

Sequences:

>Translated_412_residues
MSSEVPSRTQGADKPSVRRPAELPEGVGQATIGVRGGLLRSGFEETAEALYLTSGYVYASAAEAEKAFTGEIDRYVYSRY
GNPTISMFEERLRLIEGAPACFATATGMAAVFTSLGALLGAGDRLVAARSLFGSCFVVCSEILPRWGVETVFVDGDDLSQ
WEEALSVPTQAVFFETPSNPMQSLVDIAAVTELAHAAGAKVVLDNVFATPLLQQGFPLGVDVVVYSGTKHIDGQGRVLGG
AILGSQEYIDGPVQKLMRHTGPAISAFNAWTLLKGLETLAVRVDYSNRSAERIAEFLEGHSSVSWVKYPFLESHPQYDLA
KRQMRGGGTVVTFELAGGKERAFEVLDKLQIIDISNNLGDAKSLITHPATTTHRAMGPEGRAAIGLGDGVVRVSVGLEDT
DDLIRDLDRALG
>Mature_411_residues
SSEVPSRTQGADKPSVRRPAELPEGVGQATIGVRGGLLRSGFEETAEALYLTSGYVYASAAEAEKAFTGEIDRYVYSRYG
NPTISMFEERLRLIEGAPACFATATGMAAVFTSLGALLGAGDRLVAARSLFGSCFVVCSEILPRWGVETVFVDGDDLSQW
EEALSVPTQAVFFETPSNPMQSLVDIAAVTELAHAAGAKVVLDNVFATPLLQQGFPLGVDVVVYSGTKHIDGQGRVLGGA
ILGSQEYIDGPVQKLMRHTGPAISAFNAWTLLKGLETLAVRVDYSNRSAERIAEFLEGHSSVSWVKYPFLESHPQYDLAK
RQMRGGGTVVTFELAGGKERAFEVLDKLQIIDISNNLGDAKSLITHPATTTHRAMGPEGRAAIGLGDGVVRVSVGLEDTD
DLIRDLDRALG

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=328, Percent_Identity=32.9268292682927, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI299473759, Length=328, Percent_Identity=29.5731707317073, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI25453487, Length=327, Percent_Identity=28.1345565749235, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1790375, Length=383, Percent_Identity=33.9425587467363, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1789383, Length=340, Percent_Identity=27.0588235294118, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17560056, Length=334, Percent_Identity=35.6287425149701, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17538055, Length=332, Percent_Identity=33.1325301204819, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI71980708, Length=367, Percent_Identity=31.0626702997275, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6319307, Length=347, Percent_Identity=35.4466858789625, Blast_Score=193, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6323333, Length=410, Percent_Identity=30.7317073170732, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=375, Percent_Identity=26.9333333333333, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=270, Percent_Identity=28.1481481481481, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=340, Percent_Identity=26.1764705882353, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922554, Length=344, Percent_Identity=32.5581395348837, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48

Molecular weight: Translated: 44044; Mature: 43913

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEVPSRTQGADKPSVRRPAELPEGVGQATIGVRGGLLRSGFEETAEALYLTSGYVYAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHCCHHHHHHEEEEECCEEEEE
AAEAEKAFTGEIDRYVYSRYGNPTISMFEERLRLIEGAPACFATATGMAAVFTSLGALLG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
AGDRLVAARSLFGSCFVVCSEILPRWGVETVFVDGDDLSQWEEALSVPTQAVFFETPSNP
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCHH
MQSLVDIAAVTELAHAAGAKVVLDNVFATPLLQQGFPLGVDVVVYSGTKHIDGQGRVLGG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCEEEE
AILGSQEYIDGPVQKLMRHTGPAISAFNAWTLLKGLETLAVRVDYSNRSAERIAEFLEGH
EEECCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCC
SSVSWVKYPFLESHPQYDLAKRQMRGGGTVVTFELAGGKERAFEVLDKLQIIDISNNLGD
CCCCEEECCCCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHEEEEEECCCCCH
AKSLITHPATTTHRAMGPEGRAAIGLGDGVVRVSVGLEDTDDLIRDLDRALG
HHHHHCCCCCCCHHCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SSEVPSRTQGADKPSVRRPAELPEGVGQATIGVRGGLLRSGFEETAEALYLTSGYVYAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHCCHHHHHHEEEEECCEEEEE
AAEAEKAFTGEIDRYVYSRYGNPTISMFEERLRLIEGAPACFATATGMAAVFTSLGALLG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
AGDRLVAARSLFGSCFVVCSEILPRWGVETVFVDGDDLSQWEEALSVPTQAVFFETPSNP
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCHH
MQSLVDIAAVTELAHAAGAKVVLDNVFATPLLQQGFPLGVDVVVYSGTKHIDGQGRVLGG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCEEEE
AILGSQEYIDGPVQKLMRHTGPAISAFNAWTLLKGLETLAVRVDYSNRSAERIAEFLEGH
EEECCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCC
SSVSWVKYPFLESHPQYDLAKRQMRGGGTVVTFELAGGKERAFEVLDKLQIIDISNNLGD
CCCCEEECCCCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHEEEEEECCCCCH
AKSLITHPATTTHRAMGPEGRAAIGLGDGVVRVSVGLEDTDDLIRDLDRALG
HHHHHCCCCCCCHHCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; L-cysteine

Specific reaction: O-succinyl-L-homoserine + L-cysteine = cystathionine + succinate

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]