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The map label for this gene is purT [H]

Identifier: 108797503

GI number: 108797503

Start: 579988

End: 581274

Strand: Direct

Name: purT [H]

Synonym: Mmcs_0523

Alternate gene names: 108797503

Gene position: 579988-581274 (Clockwise)

Preceding gene: 108797502

Following gene: 108797504

Centisome position: 10.17

GC content: 72.96

Gene sequence:

>1287_bases
ATGACTGAACCAACCGACGCCGACACACCCGTCACCGACCCCGAGGTGGCGGTCCCGCAGGTCGCACCGGCCGCACCGGA
TGCGCCGGCCGAGCCCGCCGCACCGAGGAACACGGTGCTGCTACTCGGGGCAGGCGAACTCAGTCGGGAACTCGCGCTTG
CCTTTCAGCGGCTGGGTGCGTTCGTGGTGGCCGTCGACCGGTACGCCGACGCGCCCGCGCACGGGGTGGCCGATCGCTCG
GCCGTCGTGCGGCTGACCGACACCGAAGCCGTCACCGCGCTCATCGAACGGGAGAAGCCGCGCTACGTCGTCGCGCAGTC
GGGTGCCATCGCCGCCGACGCGTTGATCGCGGTGGCCGAACGCGGCGGCGTCGACGTGTTCCCGACGCCGCGAAGCACCC
GCCTGGGACAGGACCGCGAAGGGCTGCGCCGCCTGGCCTCCGATGAACTCGGCCTGCCGACGGCACCGTTCTGGTTCGCC
GGTTCGGCCGAGGAGCTCGGCACCGTCGCCCAGCACGCCGGGTTCCCGCTCGTCGTCAAACCCATCTCGGGCGCACCCGG
GGAGGGCCAGTCGGTGCTGCTGCGCGCCGAGGACGTGGAACCGGCCTGGCACCGCGCCGTCGCCGCGGGGCGCATCACCC
ACAACCGGGTGATGGCCGAGACCGTGGTCGAGGTGGATTACGAGGTGACGCTGCTGACGGTGCGCAGCACCGGCGCGACC
GGCCCGACGCTGACCTTCTGCGAACCGATCGGCCACCGCCCCGGCGACGGCGATGCGCTCGAGTCGTGGCAGCCGCAGCA
GATGACGCCCGCGGCGCTGGACGCGGCGAAGTCGATCGCCGCGCGCATGGTCAACTCGCTCGGCGGTCGGGGCGTCTTCG
CGGTCGAACTGCTGGTCCGCGGCGACGAGGTGTACTTCTCCGACGTGCGGACCCGGCTGCAGGACACCGGTCTGGTGACG
GTGCGCTCCCAGCGGCTGTCGCAGTTCGAGCTGCACGCCCGGGCGATCCTCGGTCTCGCGGTCGACACGATCATGATCTC
GCCCGGTGCGGCGGAGGTGCGGTACGCGGGCACCGAGACGTCGGTTTCCGAACCCTCGGGTGCCGACATCGCGGCGGTGG
TGGCCGACGCGCTGGCCACCCAGGAGAGCGACGTGCGGTTGTTCGGCAGGCCGGACGAGACCGACGGCCGACGCCGGCTC
GGGGTCGCGCTGGCCACCGCACCGGATGTGATCATCGCCCGTGACCGGGCCCGGCGGGTGGGCGCCGCGCTGCGCCGGCT
GTGGTGA

Upstream 100 bases:

>100_bases
ACCGGCCACCCGGTGATGCGCTCGGCCGAACCGGGGGAGTCCTGGCGGTGGTGCTACATCGACCTCCGGGTGGGTTGAGC
ATCTGGCAGGCTGGCGGGCG

Downstream 100 bases:

>100_bases
GCGATGTCCGCTGAATCCGATCCGGACACCGGCCGCCCCAGCGACCGGCCCACCGCCGCGCCGTTCATGATCGCGCTGGT
GATCATTGTGCTCGTCGTGA

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTEPTDADTPVTDPEVAVPQVAPAAPDAPAEPAAPRNTVLLLGAGELSRELALAFQRLGAFVVAVDRYADAPAHGVADRS
AVVRLTDTEAVTALIEREKPRYVVAQSGAIAADALIAVAERGGVDVFPTPRSTRLGQDREGLRRLASDELGLPTAPFWFA
GSAEELGTVAQHAGFPLVVKPISGAPGEGQSVLLRAEDVEPAWHRAVAAGRITHNRVMAETVVEVDYEVTLLTVRSTGAT
GPTLTFCEPIGHRPGDGDALESWQPQQMTPAALDAAKSIAARMVNSLGGRGVFAVELLVRGDEVYFSDVRTRLQDTGLVT
VRSQRLSQFELHARAILGLAVDTIMISPGAAEVRYAGTETSVSEPSGADIAAVVADALATQESDVRLFGRPDETDGRRRL
GVALATAPDVIIARDRARRVGAALRRLW

Sequences:

>Translated_428_residues
MTEPTDADTPVTDPEVAVPQVAPAAPDAPAEPAAPRNTVLLLGAGELSRELALAFQRLGAFVVAVDRYADAPAHGVADRS
AVVRLTDTEAVTALIEREKPRYVVAQSGAIAADALIAVAERGGVDVFPTPRSTRLGQDREGLRRLASDELGLPTAPFWFA
GSAEELGTVAQHAGFPLVVKPISGAPGEGQSVLLRAEDVEPAWHRAVAAGRITHNRVMAETVVEVDYEVTLLTVRSTGAT
GPTLTFCEPIGHRPGDGDALESWQPQQMTPAALDAAKSIAARMVNSLGGRGVFAVELLVRGDEVYFSDVRTRLQDTGLVT
VRSQRLSQFELHARAILGLAVDTIMISPGAAEVRYAGTETSVSEPSGADIAAVVADALATQESDVRLFGRPDETDGRRRL
GVALATAPDVIIARDRARRVGAALRRLW
>Mature_427_residues
TEPTDADTPVTDPEVAVPQVAPAAPDAPAEPAAPRNTVLLLGAGELSRELALAFQRLGAFVVAVDRYADAPAHGVADRSA
VVRLTDTEAVTALIEREKPRYVVAQSGAIAADALIAVAERGGVDVFPTPRSTRLGQDREGLRRLASDELGLPTAPFWFAG
SAEELGTVAQHAGFPLVVKPISGAPGEGQSVLLRAEDVEPAWHRAVAAGRITHNRVMAETVVEVDYEVTLLTVRSTGATG
PTLTFCEPIGHRPGDGDALESWQPQQMTPAALDAAKSIAARMVNSLGGRGVFAVELLVRGDEVYFSDVRTRLQDTGLVTV
RSQRLSQFELHARAILGLAVDTIMISPGAAEVRYAGTETSVSEPSGADIAAVVADALATQESDVRLFGRPDETDGRRRLG
VALATAPDVIIARDRARRVGAALRRLW

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=401, Percent_Identity=46.3840399002494, Blast_Score=318, Evalue=6e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 45227; Mature: 45095

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPTDADTPVTDPEVAVPQVAPAAPDAPAEPAAPRNTVLLLGAGELSRELALAFQRLGA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCC
FVVAVDRYADAPAHGVADRSAVVRLTDTEAVTALIEREKPRYVVAQSGAIAADALIAVAE
EEEEEEHHCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
RGGVDVFPTPRSTRLGQDREGLRRLASDELGLPTAPFWFAGSAEELGTVAQHAGFPLVVK
CCCCEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHHCCCCEEEE
PISGAPGEGQSVLLRAEDVEPAWHRAVAAGRITHNRVMAETVVEVDYEVTLLTVRSTGAT
ECCCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHEECEEEEEEEEECCCCC
GPTLTFCEPIGHRPGDGDALESWQPQQMTPAALDAAKSIAARMVNSLGGRGVFAVELLVR
CCEEEEEHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
GDEVYFSDVRTRLQDTGLVTVRSQRLSQFELHARAILGLAVDTIMISPGAAEVRYAGTET
CCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHEEHEEECCCCCEEEECCCCC
SVSEPSGADIAAVVADALATQESDVRLFGRPDETDGRRRLGVALATAPDVIIARDRARRV
CCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEECCCCEEEECHHHHHH
GAALRRLW
HHHHHHCC
>Mature Secondary Structure 
TEPTDADTPVTDPEVAVPQVAPAAPDAPAEPAAPRNTVLLLGAGELSRELALAFQRLGA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCC
FVVAVDRYADAPAHGVADRSAVVRLTDTEAVTALIEREKPRYVVAQSGAIAADALIAVAE
EEEEEEHHCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
RGGVDVFPTPRSTRLGQDREGLRRLASDELGLPTAPFWFAGSAEELGTVAQHAGFPLVVK
CCCCEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHHCCCCEEEE
PISGAPGEGQSVLLRAEDVEPAWHRAVAAGRITHNRVMAETVVEVDYEVTLLTVRSTGAT
ECCCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHEECEEEEEEEEECCCCC
GPTLTFCEPIGHRPGDGDALESWQPQQMTPAALDAAKSIAARMVNSLGGRGVFAVELLVR
CCEEEEEHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
GDEVYFSDVRTRLQDTGLVTVRSQRLSQFELHARAILGLAVDTIMISPGAAEVRYAGTET
CCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHEEHEEECCCCCEEEECCCCC
SVSEPSGADIAAVVADALATQESDVRLFGRPDETDGRRRLGVALATAPDVIIARDRARRV
CCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEECCCCEEEECHHHHHH
GAALRRLW
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA