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The map label for this gene is pilT [H]

Identifier: 108763886

GI number: 108763886

Start: 2328691

End: 2330166

Strand: Direct

Name: pilT [H]

Synonym: MXAN_1995

Alternate gene names: 108763886

Gene position: 2328691-2330166 (Clockwise)

Preceding gene: 108762003

Following gene: 108756985

Centisome position: 25.48

GC content: 68.02

Gene sequence:

>1476_bases
ATGTGTTTTCGGGGTCCATCACGCCCCATCGCATGCGCGCGCGAGCGTCTGGTAGGATTTGCGCCGCTCATGGAACTCAA
CGAGATCCTCCAGATCGCCCTGCGTGGCGGTGCCTCCGACATTCATCTCAAGGCAGGCCTGCCGCCCATGTTCCGCGTGG
ACGGTTCGCTGGTTCCGCTGAAGGACGGCCGCCGCCTCCCTCCCGAGGAGGTGGCGCGCATGGCCTTCGGCATCATGAAC
GAGTTCCAGAAGGAGAAGTTCAAGGGGAGCAACGAGGTGGACCTGGCCTACGGCGTGCCGGGGCTCGGGCGCTTCCGCGT
GAACGTCTTCCAGCAGCGTGGCACCGTGGGCGCCGTGCTGCGTGTCATCCCCTTCAAGGTGATGACCATCCAGGACCTGT
TGCTGCCCCAGATTCTCGCCAAGATTTGCGGTGAGGAGCGCGGCCTGGTCCTGGTGACGGGCACCACGGGCTCCGGCAAG
TCCACCACGCTGGCGGCGATGATCGACTACATCAACGCCAACGAGACCAGCCACATCATGACGATTGAGGACCCCATCGA
GTTCCTCATTCGCGACAAGCGCTCCATCGTGAACCAGCGCGAGGTGGGTGTGGACACGATGACCTTCGCGCAGGCGCTCA
AGAGCGCGCTGCGGCAGGACCCGGACGTCATCCTCGTGGGCGAAATGCGTGACCACGAAACCATCGAAACGGCGCTTCAC
GCCGCGGAGACGGGCCACCTGGTGATGTCCACGCTGCACACGCTGGACGCGACGGAGACCATCAACCGCATCGTCTCCGC
CTTCCCGCCGCACCAGCAGAAGCAGGTGCGCCTCCAGTTGGCCAGTGTGCTCAAGGCCGTGGTGTCCCAGCGTCTGGTGC
CGCGCGCGGACGGCAAGGGTCGCGTGGCCGCCGTGGAAGTGCTGCGCGTCACGGCGCGTGTCCGCGAGATGATTGAAGAC
AAGGACCGCACGAAGGAGATCCACGACGCCATTGCCCAGGGCACGGACACGTACGGGATGCAGACCTTCGACCAGTCGCT
GATGAGCCTGGTGCGGCAGGGGCTCGTCACCTACGAGGAGGCCCATCGGCAGGCCACCAACCCGGACGACTTCGCGCTGC
GCTTCTCCGGCATCAGCGGCACGTCCGACTCCAAGTGGGACAACTTCGATTCGAAGCCCGGCGAGTCGCGGCCCATTCCT
GGCTCGTCCGCCTTCGCGCAGAAGGGGGCGCCCACGGCTGTCGCGGCGCCGCAGGCCGCGCCTGCTCCGGCGCCCATGGC
GCAGCCCATGCGACCGGCGGCTCCAGCAGCCCAGCAGATGCGCCCGGGGGGGCCGCCGCAGGCCATGCGTCCGGGGGCTC
CGGCCGGCGCGCCGGTGGGGCGTCCCATGGCGTCTCCCGCCGCGCGCCCGCCAGCGCCCGCTCCGGCCCCCGCAGCGGCA
GGTGGGGACGACGACTTCCAGATCGAGCGCTTCTAG

Upstream 100 bases:

>100_bases
GGCGCGCGTCGCCGGTTCCAGTTCTCCAAGCGCTAAGCCACTCGCTCAGCAGCTGGGTTGTCCTCACACGGCGGGGGTCC
TCATCCGAGGGCCTCCGCCG

Downstream 100 bases:

>100_bases
CGGTTCGCGGCGCCCACCCGGAAAGGGCAGGGCGCCCGTGCCGGGCTGCTACGCGTCGTCCTCACCCAGCAAGGCCCGCA
GGCGGGACAAGCGGATGGGG

Product: twitching mobility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MCFRGPSRPIACARERLVGFAPLMELNEILQIALRGGASDIHLKAGLPPMFRVDGSLVPLKDGRRLPPEEVARMAFGIMN
EFQKEKFKGSNEVDLAYGVPGLGRFRVNVFQQRGTVGAVLRVIPFKVMTIQDLLLPQILAKICGEERGLVLVTGTTGSGK
STTLAAMIDYINANETSHIMTIEDPIEFLIRDKRSIVNQREVGVDTMTFAQALKSALRQDPDVILVGEMRDHETIETALH
AAETGHLVMSTLHTLDATETINRIVSAFPPHQQKQVRLQLASVLKAVVSQRLVPRADGKGRVAAVEVLRVTARVREMIED
KDRTKEIHDAIAQGTDTYGMQTFDQSLMSLVRQGLVTYEEAHRQATNPDDFALRFSGISGTSDSKWDNFDSKPGESRPIP
GSSAFAQKGAPTAVAAPQAAPAPAPMAQPMRPAAPAAQQMRPGGPPQAMRPGAPAGAPVGRPMASPAARPPAPAPAPAAA
GGDDDFQIERF

Sequences:

>Translated_491_residues
MCFRGPSRPIACARERLVGFAPLMELNEILQIALRGGASDIHLKAGLPPMFRVDGSLVPLKDGRRLPPEEVARMAFGIMN
EFQKEKFKGSNEVDLAYGVPGLGRFRVNVFQQRGTVGAVLRVIPFKVMTIQDLLLPQILAKICGEERGLVLVTGTTGSGK
STTLAAMIDYINANETSHIMTIEDPIEFLIRDKRSIVNQREVGVDTMTFAQALKSALRQDPDVILVGEMRDHETIETALH
AAETGHLVMSTLHTLDATETINRIVSAFPPHQQKQVRLQLASVLKAVVSQRLVPRADGKGRVAAVEVLRVTARVREMIED
KDRTKEIHDAIAQGTDTYGMQTFDQSLMSLVRQGLVTYEEAHRQATNPDDFALRFSGISGTSDSKWDNFDSKPGESRPIP
GSSAFAQKGAPTAVAAPQAAPAPAPMAQPMRPAAPAAQQMRPGGPPQAMRPGAPAGAPVGRPMASPAARPPAPAPAPAAA
GGDDDFQIERF
>Mature_491_residues
MCFRGPSRPIACARERLVGFAPLMELNEILQIALRGGASDIHLKAGLPPMFRVDGSLVPLKDGRRLPPEEVARMAFGIMN
EFQKEKFKGSNEVDLAYGVPGLGRFRVNVFQQRGTVGAVLRVIPFKVMTIQDLLLPQILAKICGEERGLVLVTGTTGSGK
STTLAAMIDYINANETSHIMTIEDPIEFLIRDKRSIVNQREVGVDTMTFAQALKSALRQDPDVILVGEMRDHETIETALH
AAETGHLVMSTLHTLDATETINRIVSAFPPHQQKQVRLQLASVLKAVVSQRLVPRADGKGRVAAVEVLRVTARVREMIED
KDRTKEIHDAIAQGTDTYGMQTFDQSLMSLVRQGLVTYEEAHRQATNPDDFALRFSGISGTSDSKWDNFDSKPGESRPIP
GSSAFAQKGAPTAVAAPQAAPAPAPMAQPMRPAAPAAQQMRPGGPPQAMRPGAPAGAPVGRPMASPAARPPAPAPAPAAA
GGDDDFQIERF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=331, Percent_Identity=39.2749244712991, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1786296, Length=390, Percent_Identity=32.3076923076923, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789723, Length=291, Percent_Identity=34.7079037800687, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 52960; Mature: 52960

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCFRGPSRPIACARERLVGFAPLMELNEILQIALRGGASDIHLKAGLPPMFRVDGSLVPL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEECCCCEEEC
KDGRRLPPEEVARMAFGIMNEFQKEKFKGSNEVDLAYGVPGLGRFRVNVFQQRGTVGAVL
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHH
RVIPFKVMTIQDLLLPQILAKICGEERGLVLVTGTTGSGKSTTLAAMIDYINANETSHIM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEE
TIEDPIEFLIRDKRSIVNQREVGVDTMTFAQALKSALRQDPDVILVGEMRDHETIETALH
EEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
AAETGHLVMSTLHTLDATETINRIVSAFPPHQQKQVRLQLASVLKAVVSQRLVPRADGKG
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RVAAVEVLRVTARVREMIEDKDRTKEIHDAIAQGTDTYGMQTFDQSLMSLVRQGLVTYEE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AHRQATNPDDFALRFSGISGTSDSKWDNFDSKPGESRPIPGSSAFAQKGAPTAVAAPQAA
HHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEECCCCC
PAPAPMAQPMRPAAPAAQQMRPGGPPQAMRPGAPAGAPVGRPMASPAARPPAPAPAPAAA
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGDDDFQIERF
CCCCCCEEECC
>Mature Secondary Structure
MCFRGPSRPIACARERLVGFAPLMELNEILQIALRGGASDIHLKAGLPPMFRVDGSLVPL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEECCCCEEEC
KDGRRLPPEEVARMAFGIMNEFQKEKFKGSNEVDLAYGVPGLGRFRVNVFQQRGTVGAVL
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHH
RVIPFKVMTIQDLLLPQILAKICGEERGLVLVTGTTGSGKSTTLAAMIDYINANETSHIM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEE
TIEDPIEFLIRDKRSIVNQREVGVDTMTFAQALKSALRQDPDVILVGEMRDHETIETALH
EEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
AAETGHLVMSTLHTLDATETINRIVSAFPPHQQKQVRLQLASVLKAVVSQRLVPRADGKG
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RVAAVEVLRVTARVREMIEDKDRTKEIHDAIAQGTDTYGMQTFDQSLMSLVRQGLVTYEE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AHRQATNPDDFALRFSGISGTSDSKWDNFDSKPGESRPIPGSSAFAQKGAPTAVAAPQAA
HHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEECCCCC
PAPAPMAQPMRPAAPAAQQMRPGGPPQAMRPGAPAGAPVGRPMASPAARPPAPAPAPAAA
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGDDDFQIERF
CCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]