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The map label for this gene is rpoD

Identifier: 108763751

GI number: 108763751

Start: 6497804

End: 6499930

Strand: Direct

Name: rpoD

Synonym: MXAN_5204

Alternate gene names: 108763751

Gene position: 6497804-6499930 (Clockwise)

Preceding gene: 108757060

Following gene: 108761246

Centisome position: 71.09

GC content: 64.46

Gene sequence:

>2127_bases
ATGCCGACGCAGAAGCCCTCGAAGGCGTCCGTGAAGCCGACCAAGAAGAAGGTGGATCCGGTGATCCGCAAGAAGAAGGC
TCCGGACGGTGCCGCGGCGAAGGGGGCCAAGACGGGCGCCGAGGAGAAGGAAGAACTCGTCGCGAGGGACGTCGTCGCCG
AGGCGACGGAGAAGCTCAAGAAGAAGAAGAAGGGCGTGGCGCCGGCCATGGGCGATGACGTGGACCCGGAGGAGGCCGCG
GAAGAGGCCGCCGCCGCCGTCCAGGTCGACCCCGACGCCGTCGAGGACGACATCGAGGAGGATCCGGTCGCCGAGCGCAA
GGAGGTCAAGGACCTGCTCGCCGCGGGCCGTGAGAAGGGCTTCCTCACGTACGACGAGGTCAATGACGCGCTCCCCGCCG
ACATCGTGTCGTCCGATCAGATCGACGACGTGATGAGCATGTTCGGCGACAACGACATCGAGATTGTCGACGCGCAGAAG
GCCGCTCAGAACAACGAAATCAAGCCCACCGTCACCGTCGAGGAAGAGAAGGAAGACGCCGACGAGGACGAGAAGGACGA
GGACGACGAGCCGGGCGGCAAGTCGAACGACCCGGTGCGCCTGTACCTGCGCAAGATGGGCAGCGTCAGCCTGCTCACCC
GCGAGGGCGAGGTCGAAATCGCCAAGCGCATCGAGGATGGTGAGAAGGAAGTCCTCCGCGCGCTGCTGGCGTGCAAGGTG
GCCGTCGAGGAGATACTCGACATCGGCAACAAGCTGAAGACGGCCAAGCTGCGCGTGCGCGACGTCATCAAGGACGCGCC
GGAGGAGACGCAGTCCGAAGGTGCCGAAGAGGCGCCCGAGGAGGTCGGTGAAGGTGACGCTCCGGCGCAGCTGGCGCAGA
GCGAGCTCAACAAGATTGAGCAGATCTGCAAGCAGATCGAGCGCTTCCGCAAGTTCGCCCAGGACTGCGACGTCTTGGAG
GAGGAGCTGTCCTCCAAGAAGAAGCTGACCGAGGTCCGCAAGAAGGAAGTGAAGCAGGAGATCAAGGACCTCCGGACCAA
GATGATGGAGGTCCTGGAGGAGATGCGGCTCAACAAGAAGCAGGTGGACCGCATCGTCATCAACCTCAAGGCCCTCATCG
AGCGCGTCGACAAGGCCGAGGACGAGCTGCGTGATTTGGAGCGCCGCTACGACTGCTCCATGAAGGAGCTGCGCCCGCAG
CTGAAGGAGTCGCGGGAGAACCCCAACATCGGCAAGAAGCTGCAGAAGCAGCTCAACCTCACGCCGGAGCAGTTGGAGGT
CCTGGACCGCGACGTGCGCACGGCGGTGCGGAAGATCAAGAAGGTCGAGGAGGAGGCCAACCTCCCCGTCGAGTCGCTGC
GCCGCAACTACGACGCCATCCGTCTGGGTGAGCGCCGCGCCGAACGCGCCAAGAGCGAGCTGGTGGAGGCCAACCTGCGC
CTCGTGGTGTCCATCGCGAAGAAGTACACGAACCGCGGCCTGCAGTTCCTGGACCTCATCCAGGAGGGCAACATCGGCCT
GATGAAGGCCGTGGACAAGTTCGAGTACAAGCGCGGCTACAAGTTCTCGACCTACGCCACCTGGTGGATTCGTCAGGCCA
TCACCCGCGCCATCGCGGACCAGGCCCGCACCATCCGCATCCCGGTGCACATGATCGAGACCATCAACAAGCTCATCCGC
ACCAGCCGCTACCTGGTGCAGGAGATTGGCCGCGAGCCGACGCCGGAGGAGATCGCGGAGAAGATGGAGCTGCCGCTCGA
CAAGGTCCGCAAGGTCCTCAAGATTGCGAAGGAGCCCATCTCCCTCGAGACGCCGATTGGCGAGGAAGAGGACAGCCACC
TGGGCGACTTCATCGAGGACAAGAGCCTCGTGTCGCCGGCGGACGCGGTCATCAACATGAACCTGGCGGAGCAGACCCGG
AAGGTGCTCGCCACGCTGACGCCGCGCGAGGAGAAGGTGCTCCGCATGCGCTTCGGCATCGGCGAGAAGTCCGACCACAC
GCTGGAAGAGGTGGGCCAGGACTTCGAGGTGACGCGCGAGCGCATCCGTCAGATTGAGGCCAAGGCGCTGCGCAAGCTGC
GCCACCCGAGCCGCTCCAAGCGCCTGCGCTCCTTCGTGGAGAGCTGA

Upstream 100 bases:

>100_bases
CGGTTTGAGTTCGCGTTTGTAAGAAACCCTGACTTTGCGGTAGATCGGCCGGCTCGCCCAGGCCTCAAGCCCCTGAATTT
GCTCAAGGAGAAGTACCCGA

Downstream 100 bases:

>100_bases
GCCGGGCTTGAGCTGAGCTGAACGTGGGCCCCCGCTCCATTCGTGGACCGGGGGCCTTCGTGTTTCTAGAGTCCGGGGCC
GTGGCGCTCCGAGCGAAGAC

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-80

Number of amino acids: Translated: 708; Mature: 707

Protein sequence:

>708_residues
MPTQKPSKASVKPTKKKVDPVIRKKKAPDGAAAKGAKTGAEEKEELVARDVVAEATEKLKKKKKGVAPAMGDDVDPEEAA
EEAAAAVQVDPDAVEDDIEEDPVAERKEVKDLLAAGREKGFLTYDEVNDALPADIVSSDQIDDVMSMFGDNDIEIVDAQK
AAQNNEIKPTVTVEEEKEDADEDEKDEDDEPGGKSNDPVRLYLRKMGSVSLLTREGEVEIAKRIEDGEKEVLRALLACKV
AVEEILDIGNKLKTAKLRVRDVIKDAPEETQSEGAEEAPEEVGEGDAPAQLAQSELNKIEQICKQIERFRKFAQDCDVLE
EELSSKKKLTEVRKKEVKQEIKDLRTKMMEVLEEMRLNKKQVDRIVINLKALIERVDKAEDELRDLERRYDCSMKELRPQ
LKESRENPNIGKKLQKQLNLTPEQLEVLDRDVRTAVRKIKKVEEEANLPVESLRRNYDAIRLGERRAERAKSELVEANLR
LVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIR
TSRYLVQEIGREPTPEEIAEKMELPLDKVRKVLKIAKEPISLETPIGEEEDSHLGDFIEDKSLVSPADAVINMNLAEQTR
KVLATLTPREEKVLRMRFGIGEKSDHTLEEVGQDFEVTRERIRQIEAKALRKLRHPSRSKRLRSFVES

Sequences:

>Translated_708_residues
MPTQKPSKASVKPTKKKVDPVIRKKKAPDGAAAKGAKTGAEEKEELVARDVVAEATEKLKKKKKGVAPAMGDDVDPEEAA
EEAAAAVQVDPDAVEDDIEEDPVAERKEVKDLLAAGREKGFLTYDEVNDALPADIVSSDQIDDVMSMFGDNDIEIVDAQK
AAQNNEIKPTVTVEEEKEDADEDEKDEDDEPGGKSNDPVRLYLRKMGSVSLLTREGEVEIAKRIEDGEKEVLRALLACKV
AVEEILDIGNKLKTAKLRVRDVIKDAPEETQSEGAEEAPEEVGEGDAPAQLAQSELNKIEQICKQIERFRKFAQDCDVLE
EELSSKKKLTEVRKKEVKQEIKDLRTKMMEVLEEMRLNKKQVDRIVINLKALIERVDKAEDELRDLERRYDCSMKELRPQ
LKESRENPNIGKKLQKQLNLTPEQLEVLDRDVRTAVRKIKKVEEEANLPVESLRRNYDAIRLGERRAERAKSELVEANLR
LVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIR
TSRYLVQEIGREPTPEEIAEKMELPLDKVRKVLKIAKEPISLETPIGEEEDSHLGDFIEDKSLVSPADAVINMNLAEQTR
KVLATLTPREEKVLRMRFGIGEKSDHTLEEVGQDFEVTRERIRQIEAKALRKLRHPSRSKRLRSFVES
>Mature_707_residues
PTQKPSKASVKPTKKKVDPVIRKKKAPDGAAAKGAKTGAEEKEELVARDVVAEATEKLKKKKKGVAPAMGDDVDPEEAAE
EAAAAVQVDPDAVEDDIEEDPVAERKEVKDLLAAGREKGFLTYDEVNDALPADIVSSDQIDDVMSMFGDNDIEIVDAQKA
AQNNEIKPTVTVEEEKEDADEDEKDEDDEPGGKSNDPVRLYLRKMGSVSLLTREGEVEIAKRIEDGEKEVLRALLACKVA
VEEILDIGNKLKTAKLRVRDVIKDAPEETQSEGAEEAPEEVGEGDAPAQLAQSELNKIEQICKQIERFRKFAQDCDVLEE
ELSSKKKLTEVRKKEVKQEIKDLRTKMMEVLEEMRLNKKQVDRIVINLKALIERVDKAEDELRDLERRYDCSMKELRPQL
KESRENPNIGKKLQKQLNLTPEQLEVLDRDVRTAVRKIKKVEEEANLPVESLRRNYDAIRLGERRAERAKSELVEANLRL
VVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRT
SRYLVQEIGREPTPEEIAEKMELPLDKVRKVLKIAKEPISLETPIGEEEDSHLGDFIEDKSLVSPADAVINMNLAEQTRK
VLATLTPREEKVLRMRFGIGEKSDHTLEEVGQDFEVTRERIRQIEAKALRKLRHPSRSKRLRSFVES

Specific function: Also required for extracellular A-signal production. The A-signal is the first in a series of extracellular signals that are required for myxspore differentiation in M.xanthus. It is thought to act as a cell-density signal during early development to ensu

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family

Homologues:

Organism=Escherichia coli, GI1789448, Length=637, Percent_Identity=46.4678178963893, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1789098, Length=234, Percent_Identity=44.4444444444444, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=28.099173553719, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.2178217821782, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): RPOD_MYXXA (P17531)

Other databases:

- EMBL:   U20669
- EMBL:   M32347
- PIR:   S70834
- ProteinModelPortal:   P17531
- SMR:   P17531
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02393
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 80400; Mature: 80268

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTQKPSKASVKPTKKKVDPVIRKKKAPDGAAAKGAKTGAEEKEELVARDVVAEATEKLK
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KKKKGVAPAMGDDVDPEEAAEEAAAAVQVDPDAVEDDIEEDPVAERKEVKDLLAAGREKG
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
FLTYDEVNDALPADIVSSDQIDDVMSMFGDNDIEIVDAQKAAQNNEIKPTVTVEEEKEDA
CEEHHHHCCCCCHHHHCCCHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCEEECCCHHCC
DEDEKDEDDEPGGKSNDPVRLYLRKMGSVSLLTREGEVEIAKRIEDGEKEVLRALLACKV
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHH
AVEEILDIGNKLKTAKLRVRDVIKDAPEETQSEGAEEAPEEVGEGDAPAQLAQSELNKIE
HHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHH
QICKQIERFRKFAQDCDVLEEELSSKKKLTEVRKKEVKQEIKDLRTKMMEVLEEMRLNKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QVDRIVINLKALIERVDKAEDELRDLERRYDCSMKELRPQLKESRENPNIGKKLQKQLNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCC
TPEQLEVLDRDVRTAVRKIKKVEEEANLPVESLRRNYDAIRLGERRAERAKSELVEANLR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIAD
HHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QARTIRIPVHMIETINKLIRTSRYLVQEIGREPTPEEIAEKMELPLDKVRKVLKIAKEPI
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCC
SLETPIGEEEDSHLGDFIEDKSLVSPADAVINMNLAEQTRKVLATLTPREEKVLRMRFGI
CCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCC
GEKSDHTLEEVGQDFEVTRERIRQIEAKALRKLRHPSRSKRLRSFVES
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
PTQKPSKASVKPTKKKVDPVIRKKKAPDGAAAKGAKTGAEEKEELVARDVVAEATEKLK
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KKKKGVAPAMGDDVDPEEAAEEAAAAVQVDPDAVEDDIEEDPVAERKEVKDLLAAGREKG
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
FLTYDEVNDALPADIVSSDQIDDVMSMFGDNDIEIVDAQKAAQNNEIKPTVTVEEEKEDA
CEEHHHHCCCCCHHHHCCCHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCEEECCCHHCC
DEDEKDEDDEPGGKSNDPVRLYLRKMGSVSLLTREGEVEIAKRIEDGEKEVLRALLACKV
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHH
AVEEILDIGNKLKTAKLRVRDVIKDAPEETQSEGAEEAPEEVGEGDAPAQLAQSELNKIE
HHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHH
QICKQIERFRKFAQDCDVLEEELSSKKKLTEVRKKEVKQEIKDLRTKMMEVLEEMRLNKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QVDRIVINLKALIERVDKAEDELRDLERRYDCSMKELRPQLKESRENPNIGKKLQKQLNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCC
TPEQLEVLDRDVRTAVRKIKKVEEEANLPVESLRRNYDAIRLGERRAERAKSELVEANLR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIAD
HHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QARTIRIPVHMIETINKLIRTSRYLVQEIGREPTPEEIAEKMELPLDKVRKVLKIAKEPI
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCC
SLETPIGEEEDSHLGDFIEDKSLVSPADAVINMNLAEQTRKVLATLTPREEKVLRMRFGI
CCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCC
GEKSDHTLEEVGQDFEVTRERIRQIEAKALRKLRHPSRSKRLRSFVES
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104614