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The map label for this gene is pfkB [H]

Identifier: 108763696

GI number: 108763696

Start: 2759273

End: 2760220

Strand: Direct

Name: pfkB [H]

Synonym: MXAN_2372

Alternate gene names: 108763696

Gene position: 2759273-2760220 (Clockwise)

Preceding gene: 108761261

Following gene: 108756954

Centisome position: 30.19

GC content: 73.31

Gene sequence:

>948_bases
ATGCCGCACATCGTCACCCTGACGCTCAACCCGACCATCGACGTGGCTACGTCCGTGCCGGAAGTCACGCCGGAGCACAA
GCTGCGCTGTGGCCCCGTGCGCAGGGACCCGGGTGGGGGCGGCATCAACGTGGCGCGCGTGGTGCGTGAGCTGGGCGGTG
AGGCCATCGCCGTCTACACGCGGGGCGGGGCCACCGGGAGCCAGTTGGAGGAGCTCCTGGAGGAGAAGGGCCTGCTGCGC
CGCTCCGTGCCGTTGCAGGACGCCACCCGGGAGAGCTTCACGGTGGCGGAAGGCACGGGCGCGCGTGAGTATCGCTTCAT
CCTGCCAGGGCCCACGCTGTCCGAGCGCGAGTGGCAGCGCTGCCTCGACGCACTGGAGGACGTGGCCGTGGGCGCGACCT
ACATCGTCGCCAGCGGCAGCCTGGCGCCCGGCGTACCGGAGGACTTCTATGCCCGTGTGGCCCGGCTGGGGAAGCGCCTG
GCCGTGCGCGTGGTGGTGGACACCAACGGTCCGCCGCTGCGGGCCGCGCTGGAAGAGGGCGTGTTCATGGCCAAGCCCAA
CCGCCGCGAGCTGCGGGACCTGAGCGGCGCGTCGGTGGAAGACTTCGCCACCCAGGCCGCCGCGGCGCGCGAGCTGGTGG
CCCAGGGCCGCGCCGAAGTGCTGGCGGTGTCCATGGGCGCGGACGGCGCGCTGGTGACGACGCGCACCGCCCAGTTCCGG
GCGACGCCGCCCCCGGTGCGGACGTACAGCTCGGTGGGCGCGGGGGACAGCTTTGTCGCGGGGATGACGCTTGCGCTCGC
GCGGGGCCAGTCCACCGAGGACGCGCTGCGCTGGGGCATCGCCTCCGGGACGGCCGCGCTGCTCACCCAGGGGACGGACC
TGTGTTACCGGGCGGACGTGGAGCGGCTCTTCACCCAGGTGAAGGCGGAGCCGGTGTCGCGTCCGTAG

Upstream 100 bases:

>100_bases
TGGGACGGAGCGGCGTGGGCGCTCGGATGGCTGGCGAATACGCGCGGGGTGGGGCAGGCTCGGCCCCTTCGCGCCGACGC
AACCCAGGAGGCCCTTCGCG

Downstream 100 bases:

>100_bases
GCACCCCGCCCGCAGAGGGAAGGAGGGCTTGCCCCTTCCATTCCCAGGAGTGGCGGCGGCGAGGCCGAATTCGGGGTGCG
AGGTGGTGGCGGCGCGAACA

Product: 6-phosphofructokinase

Products: ADP; D-fructose 1,6-bisphosphate

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MPHIVTLTLNPTIDVATSVPEVTPEHKLRCGPVRRDPGGGGINVARVVRELGGEAIAVYTRGGATGSQLEELLEEKGLLR
RSVPLQDATRESFTVAEGTGAREYRFILPGPTLSEREWQRCLDALEDVAVGATYIVASGSLAPGVPEDFYARVARLGKRL
AVRVVVDTNGPPLRAALEEGVFMAKPNRRELRDLSGASVEDFATQAAAARELVAQGRAEVLAVSMGADGALVTTRTAQFR
ATPPPVRTYSSVGAGDSFVAGMTLALARGQSTEDALRWGIASGTAALLTQGTDLCYRADVERLFTQVKAEPVSRP

Sequences:

>Translated_315_residues
MPHIVTLTLNPTIDVATSVPEVTPEHKLRCGPVRRDPGGGGINVARVVRELGGEAIAVYTRGGATGSQLEELLEEKGLLR
RSVPLQDATRESFTVAEGTGAREYRFILPGPTLSEREWQRCLDALEDVAVGATYIVASGSLAPGVPEDFYARVARLGKRL
AVRVVVDTNGPPLRAALEEGVFMAKPNRRELRDLSGASVEDFATQAAAARELVAQGRAEVLAVSMGADGALVTTRTAQFR
ATPPPVRTYSSVGAGDSFVAGMTLALARGQSTEDALRWGIASGTAALLTQGTDLCYRADVERLFTQVKAEPVSRP
>Mature_314_residues
PHIVTLTLNPTIDVATSVPEVTPEHKLRCGPVRRDPGGGGINVARVVRELGGEAIAVYTRGGATGSQLEELLEEKGLLRR
SVPLQDATRESFTVAEGTGAREYRFILPGPTLSEREWQRCLDALEDVAVGATYIVASGSLAPGVPEDFYARVARLGKRLA
VRVVVDTNGPPLRAALEEGVFMAKPNRRELRDLSGASVEDFATQAAAARELVAQGRAEVLAVSMGADGALVTTRTAQFRA
TPPPVRTYSSVGAGDSFVAGMTLALARGQSTEDALRWGIASGTAALLTQGTDLCYRADVERLFTQVKAEPVSRP

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=305, Percent_Identity=39.672131147541, Blast_Score=220, Evalue=9e-59,
Organism=Escherichia coli, GI1788493, Length=276, Percent_Identity=31.5217391304348, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.11

Molecular weight: Translated: 33450; Mature: 33319

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHIVTLTLNPTIDVATSVPEVTPEHKLRCGPVRRDPGGGGINVARVVRELGGEAIAVYT
CCEEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEE
RGGATGSQLEELLEEKGLLRRSVPLQDATRESFTVAEGTGAREYRFILPGPTLSEREWQR
CCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCHHHHHH
CLDALEDVAVGATYIVASGSLAPGVPEDFYARVARLGKRLAVRVVVDTNGPPLRAALEEG
HHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHCC
VFMAKPNRRELRDLSGASVEDFATQAAAARELVAQGRAEVLAVSMGADGALVTTRTAQFR
EEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCEEE
ATPPPVRTYSSVGAGDSFVAGMTLALARGQSTEDALRWGIASGTAALLTQGTDLCYRADV
CCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHHH
ERLFTQVKAEPVSRP
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
PHIVTLTLNPTIDVATSVPEVTPEHKLRCGPVRRDPGGGGINVARVVRELGGEAIAVYT
CEEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEE
RGGATGSQLEELLEEKGLLRRSVPLQDATRESFTVAEGTGAREYRFILPGPTLSEREWQR
CCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCHHHHHH
CLDALEDVAVGATYIVASGSLAPGVPEDFYARVARLGKRLAVRVVVDTNGPPLRAALEEG
HHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHCC
VFMAKPNRRELRDLSGASVEDFATQAAAARELVAQGRAEVLAVSMGADGALVTTRTAQFR
EEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCEEE
ATPPPVRTYSSVGAGDSFVAGMTLALARGQSTEDALRWGIASGTAALLTQGTDLCYRADV
CCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHHH
ERLFTQVKAEPVSRP
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-fructose 6-phosphate

Specific reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]