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The map label for this gene is ycjG [C]

Identifier: 108763693

GI number: 108763693

Start: 367920

End: 368996

Strand: Reverse

Name: ycjG [C]

Synonym: MXAN_0313

Alternate gene names: 108763693

Gene position: 368996-367920 (Counterclockwise)

Preceding gene: 108761025

Following gene: 108757639

Centisome position: 4.04

GC content: 70.84

Gene sequence:

>1077_bases
ATGACGCCCACCCTCATCACCGCCGTCCACTTCGAGCCGCTGCACCTCCCGCTCACCGAGCCCTTCGCCATCGCCACCGG
CGCGCAGTACGCCGCGGAGAACGTCCTGGTGCGACTCACCCTGGCGGACGGCTCCGAGGGCCTGGGCGAGGCCGCCCCCT
TCCCCGCCGTCAGTGGAGAAACACAGGCCAGTACGCTGGCCGCCCTGGAACAGGTGCGCGCGGGGCTGGTGGGACGAGAC
GCTCGCGCGTGGCGGCCCGCCTCCGAGGCGCTGGGGGATTCACTCGCGCTGGCGCCCTCCGCCCGGTGCGCCGTGGAGAC
GGCGCTGCTCGATGCGCTGGCTCGGCACTACGGCGTTCCGTTGTACGTGATGCTGGGCGGCGCGCAGTCCGCGCTGGACA
TCGACATGACGGTCACCGCGGGAGACGTGGCCCATGCGCGAAAATCCGCGCAGGCAATCCTGGCGCGGGGCATCACCACG
CTGAAGGTGAAGGTCGGGGCGCTGCCGCCCGTCGAGGACGCGGCACGACTGGTGGCCATTCATGAAGTCGCGCCCCAGGC
CCGGCTCTTCGCGGATGCCAACGGTGGCTATGACGTGGCCGAGGCGCTCGCCTTCTTGAAAGAACTGGCGCGCGCGGACG
TCCCCCTGGCCCTCTTCGAACAACCCGTGCCCGCGCATGATGTGGCCGGTATGGCGGAGCTCACGCGCCGCTCGCGCGTG
CCGGTGTGCGCGGATGAGTCGGCGCGCTCGGCGAAGGACGTGCTGCGACTCATCCGGGAAGGCGCCGCGCACGCCATCAA
CATCAAGACGATGAAGTGCGGCGTCGTCGAGGCACTGACGATGTGGAGCCTCGCTCGCGCAGCGGGCCTGGAGCTCATGG
TGGGCGGCATGGTGGAGAGCGTGCTGGCCATGTCCGCCTCGGCGCACCTGGCCGCCGGCTTGGGGGGCTTCACCTACGCC
GACCTGGATACACCGCTGTTCATCGCGAGCCACCCCTTCCAAGGCGGCGCCCGATACGCGGGCGCACGGCTGGAGTTGCC
AACGGATGCACCCGGGCACGGCGTCACGCTGGCCTGA

Upstream 100 bases:

>100_bases
ATGTATCAGTATCAGTTCCCATGTGTCTGTCGGCCAACCGCCGCTTGACCTGGCTCGCGCGCGCAGGGGACGCCCGGGCG
GCTAGGCTCCTCGGCCGCCC

Downstream 100 bases:

>100_bases
GCCGCGCTCCGCCGCACGGGCTGCGGTCCTGCGAACCCGGCCCGCTGCCTCCACCCGCCGGGCCGTCGTTGCCATGAATG
GTGGCGATGTGAATCTGGTT

Product: mandelate racemase/muconate lactonizing enzyme family protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MTPTLITAVHFEPLHLPLTEPFAIATGAQYAAENVLVRLTLADGSEGLGEAAPFPAVSGETQASTLAALEQVRAGLVGRD
ARAWRPASEALGDSLALAPSARCAVETALLDALARHYGVPLYVMLGGAQSALDIDMTVTAGDVAHARKSAQAILARGITT
LKVKVGALPPVEDAARLVAIHEVAPQARLFADANGGYDVAEALAFLKELARADVPLALFEQPVPAHDVAGMAELTRRSRV
PVCADESARSAKDVLRLIREGAAHAINIKTMKCGVVEALTMWSLARAAGLELMVGGMVESVLAMSASAHLAAGLGGFTYA
DLDTPLFIASHPFQGGARYAGARLELPTDAPGHGVTLA

Sequences:

>Translated_358_residues
MTPTLITAVHFEPLHLPLTEPFAIATGAQYAAENVLVRLTLADGSEGLGEAAPFPAVSGETQASTLAALEQVRAGLVGRD
ARAWRPASEALGDSLALAPSARCAVETALLDALARHYGVPLYVMLGGAQSALDIDMTVTAGDVAHARKSAQAILARGITT
LKVKVGALPPVEDAARLVAIHEVAPQARLFADANGGYDVAEALAFLKELARADVPLALFEQPVPAHDVAGMAELTRRSRV
PVCADESARSAKDVLRLIREGAAHAINIKTMKCGVVEALTMWSLARAAGLELMVGGMVESVLAMSASAHLAAGLGGFTYA
DLDTPLFIASHPFQGGARYAGARLELPTDAPGHGVTLA
>Mature_357_residues
TPTLITAVHFEPLHLPLTEPFAIATGAQYAAENVLVRLTLADGSEGLGEAAPFPAVSGETQASTLAALEQVRAGLVGRDA
RAWRPASEALGDSLALAPSARCAVETALLDALARHYGVPLYVMLGGAQSALDIDMTVTAGDVAHARKSAQAILARGITTL
KVKVGALPPVEDAARLVAIHEVAPQARLFADANGGYDVAEALAFLKELARADVPLALFEQPVPAHDVAGMAELTRRSRVP
VCADESARSAKDVLRLIREGAAHAINIKTMKCGVVEALTMWSLARAAGLELMVGGMVESVLAMSASAHLAAGLGGFTYAD
LDTPLFIASHPFQGGARYAGARLELPTDAPGHGVTLA

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of a broad range of other

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=319, Percent_Identity=33.8557993730408, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 37054; Mature: 36923

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTLITAVHFEPLHLPLTEPFAIATGAQYAAENVLVRLTLADGSEGLGEAAPFPAVSGE
CCCCEEEEEEECCEECCCCCCEEEECCCHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCC
TQASTLAALEQVRAGLVGRDARAWRPASEALGDSLALAPSARCAVETALLDALARHYGVP
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEECCCCHHHHHHHHHHHHHHHHCCCE
LYVMLGGAQSALDIDMTVTAGDVAHARKSAQAILARGITTLKVKVGALPPVEDAARLVAI
EEEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHH
HEVAPQARLFADANGGYDVAEALAFLKELARADVPLALFEQPVPAHDVAGMAELTRRSRV
HHHCCCHHEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHCCC
PVCADESARSAKDVLRLIREGAAHAINIKTMKCGVVEALTMWSLARAAGLELMVGGMVES
CCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VLAMSASAHLAAGLGGFTYADLDTPLFIASHPFQGGARYAGARLELPTDAPGHGVTLA
HHHHHHHHHHHHCCCCCEEECCCCCEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TPTLITAVHFEPLHLPLTEPFAIATGAQYAAENVLVRLTLADGSEGLGEAAPFPAVSGE
CCCEEEEEEECCEECCCCCCEEEECCCHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCC
TQASTLAALEQVRAGLVGRDARAWRPASEALGDSLALAPSARCAVETALLDALARHYGVP
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEECCCCHHHHHHHHHHHHHHHHCCCE
LYVMLGGAQSALDIDMTVTAGDVAHARKSAQAILARGITTLKVKVGALPPVEDAARLVAI
EEEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHH
HEVAPQARLFADANGGYDVAEALAFLKELARADVPLALFEQPVPAHDVAGMAELTRRSRV
HHHCCCHHEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHCCC
PVCADESARSAKDVLRLIREGAAHAINIKTMKCGVVEALTMWSLARAAGLELMVGGMVES
CCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VLAMSASAHLAAGLGGFTYADLDTPLFIASHPFQGGARYAGARLELPTDAPGHGVTLA
HHHHHHHHHHHHCCCCCEEECCCCCEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]