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The map label for this gene is cmlA [H]

Identifier: 108763563

GI number: 108763563

Start: 2985752

End: 2986987

Strand: Reverse

Name: cmlA [H]

Synonym: MXAN_2566

Alternate gene names: 108763563

Gene position: 2986987-2985752 (Counterclockwise)

Preceding gene: 108762679

Following gene: 108760624

Centisome position: 32.68

GC content: 68.45

Gene sequence:

>1236_bases
GTGCCTGACTTCAAATCCCCGTCGTGGAATCACGCTGTGCCAGCAGCGCTGTTGCTGATGGCGCCCTTCGACCTCCTGGC
CTCACTGGCCATGGACATCTACCTGCCAGTCGTTCCGGCGATGCCCGGAATCCTCAACACCTCTCCCGCCATCGTCCAGC
TCACCTTGAGCCTGTACATGGCGGTGCTTGGGCTCGGCCAGATGGTGTTTGGCCCGGTCTCCGACCGCATCGGCCGGCGC
CGAGTGCTGCTGACAGGCGCCCTCCTGTTCGCGGTCACGTCCTTCCTGCTCGCGGGGGCCTCGGATGCCGCGATGTTCGT
CGGCCTTCGGCTCGTCCAAGCCATGGGGGCATCCGCGGCCCTCGTCGCCACGTTCGCGACGGTCCGCGATGTCTACGCGG
AGCGCCCCGAGAGCGCGACCCTCTACAGCCTGTTCAGCGCCATGCTGGCCTTCGTCCCCGCGCTCGGGCCCATCGCGGGG
GCGCTGCTCATGCAGCGCTGGGGATGGCGAGCCATCTTCATCACACTCGGAGTCCTGGCCACGGCGGCGCTGCTCCAAGC
CTTGCCGCGGTGGCACGAGACGCGCCCGGCGCAGGGCCTTCAGTCACGTCCCGCGTTCCGGCACATCCTGCGCAGCGCCA
CGTTCTGGACGTACACCCTGGGCTTCAGCGCGGCCATGGGCTCCTTCTTCGTGTTCTTCTCCACGGCGCCCCGGGTGCTC
ATCGGTCAGGCCGGCTTCTCCGAGCTCGGGTTCAGCCTGGCGTTCGCTACCGCCGCGCTCGCGATGATTCTGACGACCCG
CTTCGCGAAGCGCTTCGTGGGGAGTTGGGGGCTGGCGGGTAGCCTCACGCGGGGCATGGGCATGTTGCTGCTCGGGGCGG
GGCTCCTGACGGCCGGACAGCTCCTGGCAACGCCGTCGTTCTGGACGTTCGTCGCGCCGATGTGGCTCATCGCGGCCGGC
ATCGTGTTCTCCGTCTCCGTCACCGCGAATGGCGCACTCCAGGCCTTTGGCGATGTGGCGGGAACGGCGGTCGCGCTCTA
CTTCTGCATCCAGAGCCTCATCGTCGGCGTCCTCGGCACGCTGATGGTCGTCCTGCTGGACGGCGACACGGCGTGGCCCC
TGGTGGGATACGCGTCCCTGATGGCAGGGGTGACGTTGACGGCGCTTCAGCGCCTCCAGCGCCGACAACGCGTCCTGACG
AAACCCGCGATGGCGGAAAGCCCCGACGCCCCCTAG

Upstream 100 bases:

>100_bases
GACACGTCAACCGTTGCCGCGGCCATCTGCCCCAGCTCATCCCGGTACCCGTGGAGAGGCAGAGCGCCGCCAGATGTTCC
TCATTTGTGCGGATGCAATC

Downstream 100 bases:

>100_bases
CAAACGTCCCCAAACCGCGCCGCGGGGGCCTGCTCCCGCGGCGCGGCGGTGGTTTCCACCTTCCCCGCCTGGGACGGAAC
CCTGCCCGCCTCCCGGCTGT

Product: Bcr/CflA family protein drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MPDFKSPSWNHAVPAALLLMAPFDLLASLAMDIYLPVVPAMPGILNTSPAIVQLTLSLYMAVLGLGQMVFGPVSDRIGRR
RVLLTGALLFAVTSFLLAGASDAAMFVGLRLVQAMGASAALVATFATVRDVYAERPESATLYSLFSAMLAFVPALGPIAG
ALLMQRWGWRAIFITLGVLATAALLQALPRWHETRPAQGLQSRPAFRHILRSATFWTYTLGFSAAMGSFFVFFSTAPRVL
IGQAGFSELGFSLAFATAALAMILTTRFAKRFVGSWGLAGSLTRGMGMLLLGAGLLTAGQLLATPSFWTFVAPMWLIAAG
IVFSVSVTANGALQAFGDVAGTAVALYFCIQSLIVGVLGTLMVVLLDGDTAWPLVGYASLMAGVTLTALQRLQRRQRVLT
KPAMAESPDAP

Sequences:

>Translated_411_residues
MPDFKSPSWNHAVPAALLLMAPFDLLASLAMDIYLPVVPAMPGILNTSPAIVQLTLSLYMAVLGLGQMVFGPVSDRIGRR
RVLLTGALLFAVTSFLLAGASDAAMFVGLRLVQAMGASAALVATFATVRDVYAERPESATLYSLFSAMLAFVPALGPIAG
ALLMQRWGWRAIFITLGVLATAALLQALPRWHETRPAQGLQSRPAFRHILRSATFWTYTLGFSAAMGSFFVFFSTAPRVL
IGQAGFSELGFSLAFATAALAMILTTRFAKRFVGSWGLAGSLTRGMGMLLLGAGLLTAGQLLATPSFWTFVAPMWLIAAG
IVFSVSVTANGALQAFGDVAGTAVALYFCIQSLIVGVLGTLMVVLLDGDTAWPLVGYASLMAGVTLTALQRLQRRQRVLT
KPAMAESPDAP
>Mature_410_residues
PDFKSPSWNHAVPAALLLMAPFDLLASLAMDIYLPVVPAMPGILNTSPAIVQLTLSLYMAVLGLGQMVFGPVSDRIGRRR
VLLTGALLFAVTSFLLAGASDAAMFVGLRLVQAMGASAALVATFATVRDVYAERPESATLYSLFSAMLAFVPALGPIAGA
LLMQRWGWRAIFITLGVLATAALLQALPRWHETRPAQGLQSRPAFRHILRSATFWTYTLGFSAAMGSFFVFFSTAPRVLI
GQAGFSELGFSLAFATAALAMILTTRFAKRFVGSWGLAGSLTRGMGMLLLGAGLLTAGQLLATPSFWTFVAPMWLIAAGI
VFSVSVTANGALQAFGDVAGTAVALYFCIQSLIVGVLGTLMVVLLDGDTAWPLVGYASLMAGVTLTALQRLQRRQRVLTK
PAMAESPDAP

Specific function: Appears to provoke a reduction of the content of the major porins ompA and ompC [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI48994889, Length=399, Percent_Identity=31.8295739348371, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87082312, Length=351, Percent_Identity=28.4900284900285, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788509, Length=383, Percent_Identity=26.8929503916449, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790146, Length=322, Percent_Identity=28.2608695652174, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1790794, Length=303, Percent_Identity=26.7326732673267, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324264, Length=202, Percent_Identity=29.7029702970297, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6322071, Length=166, Percent_Identity=27.1084337349398, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43502; Mature: 43371

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDFKSPSWNHAVPAALLLMAPFDLLASLAMDIYLPVVPAMPGILNTSPAIVQLTLSLYM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AVLGLGQMVFGPVSDRIGRRRVLLTGALLFAVTSFLLAGASDAAMFVGLRLVQAMGASAA
HHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHH
LVATFATVRDVYAERPESATLYSLFSAMLAFVPALGPIAGALLMQRWGWRAIFITLGVLA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TAALLQALPRWHETRPAQGLQSRPAFRHILRSATFWTYTLGFSAAMGSFFVFFSTAPRVL
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IGQAGFSELGFSLAFATAALAMILTTRFAKRFVGSWGLAGSLTRGMGMLLLGAGLLTAGQ
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LLATPSFWTFVAPMWLIAAGIVFSVSVTANGALQAFGDVAGTAVALYFCIQSLIVGVLGT
HHCCCCHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMVVLLDGDTAWPLVGYASLMAGVTLTALQRLQRRQRVLTKPAMAESPDAP
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PDFKSPSWNHAVPAALLLMAPFDLLASLAMDIYLPVVPAMPGILNTSPAIVQLTLSLYM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AVLGLGQMVFGPVSDRIGRRRVLLTGALLFAVTSFLLAGASDAAMFVGLRLVQAMGASAA
HHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHH
LVATFATVRDVYAERPESATLYSLFSAMLAFVPALGPIAGALLMQRWGWRAIFITLGVLA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TAALLQALPRWHETRPAQGLQSRPAFRHILRSATFWTYTLGFSAAMGSFFVFFSTAPRVL
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IGQAGFSELGFSLAFATAALAMILTTRFAKRFVGSWGLAGSLTRGMGMLLLGAGLLTAGQ
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LLATPSFWTFVAPMWLIAAGIVFSVSVTANGALQAFGDVAGTAVALYFCIQSLIVGVLGT
HHCCCCHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMVVLLDGDTAWPLVGYASLMAGVTLTALQRLQRRQRVLTKPAMAESPDAP
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1648560; 1658833 [H]