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The map label for this gene is gyrB [H]

Identifier: 108763546

GI number: 108763546

Start: 2893093

End: 2895024

Strand: Reverse

Name: gyrB [H]

Synonym: MXAN_2483

Alternate gene names: 108763546

Gene position: 2895024-2893093 (Counterclockwise)

Preceding gene: 108763597

Following gene: 108760494

Centisome position: 31.68

GC content: 67.03

Gene sequence:

>1932_bases
ATGGCGACGAAGAAGGAAACCTACACAGGCGCGGACATCCAGGTCCTGGAAGGCCTGGAGCCGGTGCGCAAGCGCCCGGC
CATGTACATCGGCGGCACCGACAGCACGGGGTATCACCACCTGCTGTGGGAGATCCTCGACAACTCGGTGGACGAGGTCA
TCAACGGCTTCGCCACCACCGTGGAGGTGACGCTCCACAAGGACGGCCGCAGCGTCACCATCGTGGACAACGGGCGTGGC
ATCCCCGTTGACATCATGCCCAAATACAAGAAGCCGGCCGTGGAGGTCATCCTCACGACCCTTCACGCGGGCGGCAAGTT
CGAGCAGGGCAACTACATCCACTCCGGCGGTCTGCACGGCGTGGGCAGCTCGGTGGTGAACGCGCTGGCGCGCAAGCTCG
TCGTGGAGATCAAGCTCCACGGCAAGAAGCACGTGCAGACGTTCGCGCGCGGCAAGGCCACCAGCACGCTGAAGGTGGAT
GGCGCCGCGCGTGGCACGGGCACCTCCGTCACGTTTGAACCGGACCCGGAGATTTTCGGCGAGAAGCTGAAGTTCGACGC
GGAGCTGGTGCGCGAGCGGCTGGAGGCCAAGAGCTACCTGCACAAGGGCATGACGGTCGTCTGGAAGGACGAGACGTCCA
GCCCGCCCACGTCGGTGACGTACAAGCACGACGGCGGCATCGCGGAGTACCTCACCAAGGTGGTGACGGAGCGGAACAAG
CCGCTGGTGCCCGCGGGCAGCGCGGCCTTCTACCACGCGCGTGACAACGGCGTGCGGCTGGAGGCGGCGCTGGCGTGGAC
GGAGGCCACCGACGAGCACATCCGCTCGTATGTCAACGGCATCCCCACCCCACTGGGCGGCACGCACGAGGCGGGTCTGC
GCAGCGCGGTGGTGAAGGCGGTGCGCAACTACATCGAGACGCACGGCATCGCGCCCAAGGGCGTGACGCTCACCGCGGAG
GACATCCGCGAGGGCATCACCGCCATCCTGTCCACCTACGTGGTGGAGCCGCAGTTCCAGGGCCAGACGAAGGGGCGGCT
CAACAACCCCGAGGTGTCCGGCCAGGTGGACGGCGTGCTGCGTCCGGCGCTGGAGAAGTGGCTCAACGACAACAAGTCCA
TCGCCGAGTCAGTGGTGGCCCGCATCGTCCTTGCCGCTCGCGCGCGCGAGGCCAGCCGGGCCGCGTCGCAGGCGGTGAGC
CGCAAGACGGCGGTCAGCCACCGGCTCAACCTGCCGGGCAAGCTGGCGGACTGCTCGTCCACGGACCCGGGCCTGAGCGA
GCTGTTCATCGTGGAAGGTGACTCCGCAGGCGGCTCCGCCAAGCAGGGCCGGGACAGGCGCACCCAGGCCATCCTCCCGC
TGCGCGGCAAGGTGCTCAACGCGGAGCAGGCGTCCACCGACAAGGTGACGACGAACAAGGAGCTCCAGGACATCGTGTCC
GCGCTGGGCTGCGGCATCGGCTCCGACTTCGACATCAGCAAGCTGCGCTACGGCCGCGTCTTCCTGCTGATGGACGCCGA
CAGCGACGGCCACCACATCGCCACGCTGCTGCTCACCTTCTTCTACCGGCACCTGCGCCCGCTGATTGAGAGCGGCGCCA
TCCACATCGCCCAGCCGCCGCTGTACCGCGTGGACATCGGCAAGGAGACGTACTGGGCGCTGGATGAGCCGGACCGCGAC
CGCATCATCAAGGAGAAGACGAAGGGCAACGCCAAGCCCAACATCATGCGCTTCAAGGGACTGGGCGAGATGACGCCCGA
CGAGCTGAAGGAGACGACGCTCGACCCGAAGCACCGCATGAGCCTGCGCGTCACCATCGACAAGCCCCTGGAGACGGACC
GGCTCATCAACGACTTGATGGGCAAGGACGTCAGCGCCCGCTTCAGGTTCATCATGGAGCGCGCCAGCGAGGTCCAGGAC
CTGGACGTCTAG

Upstream 100 bases:

>100_bases
CAAGGGCCATGAGATGTCGAAGCGCGACGCCGTGAAGGAGGTGGCGCGTGCCGTCACCTTCATCCCGTTGCCCGAGAAGA
AGGACTAGGCAGAGGACGCC

Downstream 100 bases:

>100_bases
TCAGGACGGAATCACCGTCGCGGGTCCCTCCCACCCCGGAGGGGCCCGCGCGCAGCAGGGCTTTTGAGACGGAGCCCCGG
CTCGGCTAGCATTCGCGCCG

Product: type II DNA topoisomerase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MATKKETYTGADIQVLEGLEPVRKRPAMYIGGTDSTGYHHLLWEILDNSVDEVINGFATTVEVTLHKDGRSVTIVDNGRG
IPVDIMPKYKKPAVEVILTTLHAGGKFEQGNYIHSGGLHGVGSSVVNALARKLVVEIKLHGKKHVQTFARGKATSTLKVD
GAARGTGTSVTFEPDPEIFGEKLKFDAELVRERLEAKSYLHKGMTVVWKDETSSPPTSVTYKHDGGIAEYLTKVVTERNK
PLVPAGSAAFYHARDNGVRLEAALAWTEATDEHIRSYVNGIPTPLGGTHEAGLRSAVVKAVRNYIETHGIAPKGVTLTAE
DIREGITAILSTYVVEPQFQGQTKGRLNNPEVSGQVDGVLRPALEKWLNDNKSIAESVVARIVLAARAREASRAASQAVS
RKTAVSHRLNLPGKLADCSSTDPGLSELFIVEGDSAGGSAKQGRDRRTQAILPLRGKVLNAEQASTDKVTTNKELQDIVS
ALGCGIGSDFDISKLRYGRVFLLMDADSDGHHIATLLLTFFYRHLRPLIESGAIHIAQPPLYRVDIGKETYWALDEPDRD
RIIKEKTKGNAKPNIMRFKGLGEMTPDELKETTLDPKHRMSLRVTIDKPLETDRLINDLMGKDVSARFRFIMERASEVQD
LDV

Sequences:

>Translated_643_residues
MATKKETYTGADIQVLEGLEPVRKRPAMYIGGTDSTGYHHLLWEILDNSVDEVINGFATTVEVTLHKDGRSVTIVDNGRG
IPVDIMPKYKKPAVEVILTTLHAGGKFEQGNYIHSGGLHGVGSSVVNALARKLVVEIKLHGKKHVQTFARGKATSTLKVD
GAARGTGTSVTFEPDPEIFGEKLKFDAELVRERLEAKSYLHKGMTVVWKDETSSPPTSVTYKHDGGIAEYLTKVVTERNK
PLVPAGSAAFYHARDNGVRLEAALAWTEATDEHIRSYVNGIPTPLGGTHEAGLRSAVVKAVRNYIETHGIAPKGVTLTAE
DIREGITAILSTYVVEPQFQGQTKGRLNNPEVSGQVDGVLRPALEKWLNDNKSIAESVVARIVLAARAREASRAASQAVS
RKTAVSHRLNLPGKLADCSSTDPGLSELFIVEGDSAGGSAKQGRDRRTQAILPLRGKVLNAEQASTDKVTTNKELQDIVS
ALGCGIGSDFDISKLRYGRVFLLMDADSDGHHIATLLLTFFYRHLRPLIESGAIHIAQPPLYRVDIGKETYWALDEPDRD
RIIKEKTKGNAKPNIMRFKGLGEMTPDELKETTLDPKHRMSLRVTIDKPLETDRLINDLMGKDVSARFRFIMERASEVQD
LDV
>Mature_642_residues
ATKKETYTGADIQVLEGLEPVRKRPAMYIGGTDSTGYHHLLWEILDNSVDEVINGFATTVEVTLHKDGRSVTIVDNGRGI
PVDIMPKYKKPAVEVILTTLHAGGKFEQGNYIHSGGLHGVGSSVVNALARKLVVEIKLHGKKHVQTFARGKATSTLKVDG
AARGTGTSVTFEPDPEIFGEKLKFDAELVRERLEAKSYLHKGMTVVWKDETSSPPTSVTYKHDGGIAEYLTKVVTERNKP
LVPAGSAAFYHARDNGVRLEAALAWTEATDEHIRSYVNGIPTPLGGTHEAGLRSAVVKAVRNYIETHGIAPKGVTLTAED
IREGITAILSTYVVEPQFQGQTKGRLNNPEVSGQVDGVLRPALEKWLNDNKSIAESVVARIVLAARAREASRAASQAVSR
KTAVSHRLNLPGKLADCSSTDPGLSELFIVEGDSAGGSAKQGRDRRTQAILPLRGKVLNAEQASTDKVTTNKELQDIVSA
LGCGIGSDFDISKLRYGRVFLLMDADSDGHHIATLLLTFFYRHLRPLIESGAIHIAQPPLYRVDIGKETYWALDEPDRDR
IIKEKTKGNAKPNIMRFKGLGEMTPDELKETTLDPKHRMSLRVTIDKPLETDRLINDLMGKDVSARFRFIMERASEVQDL
DV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=591, Percent_Identity=25.7191201353638, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI19913406, Length=594, Percent_Identity=24.2424242424242, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1789408, Length=643, Percent_Identity=43.701399688958, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI48994957, Length=561, Percent_Identity=44.5632798573975, Blast_Score=445, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17535065, Length=630, Percent_Identity=24.1269841269841, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI212645845, Length=563, Percent_Identity=22.9129662522202, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI212645657, Length=230, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=630, Percent_Identity=25.0793650793651, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI17136538, Length=667, Percent_Identity=23.0884557721139, Blast_Score=115, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 70426; Mature: 70295

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKKETYTGADIQVLEGLEPVRKRPAMYIGGTDSTGYHHLLWEILDNSVDEVINGFATT
CCCCCCCCCCCCHHHHHCCHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
VEVTLHKDGRSVTIVDNGRGIPVDIMPKYKKPAVEVILTTLHAGGKFEQGNYIHSGGLHG
EEEEEECCCCEEEEEECCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCC
VGSSVVNALARKLVVEIKLHGKKHVQTFARGKATSTLKVDGAARGTGTSVTFEPDPEIFG
CHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCEEEECCCCCCCCCEEEECCCHHHHH
EKLKFDAELVRERLEAKSYLHKGMTVVWKDETSSPPTSVTYKHDGGIAEYLTKVVTERNK
HHHCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCC
PLVPAGSAAFYHARDNGVRLEAALAWTEATDEHIRSYVNGIPTPLGGTHEAGLRSAVVKA
CCCCCCCCEEEEECCCCEEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VRNYIETHGIAPKGVTLTAEDIREGITAILSTYVVEPQFQGQTKGRLNNPEVSGQVDGVL
HHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
RPALEKWLNDNKSIAESVVARIVLAARAREASRAASQAVSRKTAVSHRLNLPGKLADCSS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TDPGLSELFIVEGDSAGGSAKQGRDRRTQAILPLRGKVLNAEQASTDKVTTNKELQDIVS
CCCCCEEEEEEECCCCCCCCCCCCCHHHHEECCCCCCEECCCCCCCCCCCCCHHHHHHHH
ALGCGIGSDFDISKLRYGRVFLLMDADSDGHHIATLLLTFFYRHLRPLIESGAIHIAQPP
HHCCCCCCCCCHHHEEECEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
LYRVDIGKETYWALDEPDRDRIIKEKTKGNAKPNIMRFKGLGEMTPDELKETTLDPKHRM
EEEEECCCCEEEECCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCE
SLRVTIDKPLETDRLINDLMGKDVSARFRFIMERASEVQDLDV
EEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATKKETYTGADIQVLEGLEPVRKRPAMYIGGTDSTGYHHLLWEILDNSVDEVINGFATT
CCCCCCCCCCCHHHHHCCHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
VEVTLHKDGRSVTIVDNGRGIPVDIMPKYKKPAVEVILTTLHAGGKFEQGNYIHSGGLHG
EEEEEECCCCEEEEEECCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCC
VGSSVVNALARKLVVEIKLHGKKHVQTFARGKATSTLKVDGAARGTGTSVTFEPDPEIFG
CHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCEEEECCCCCCCCCEEEECCCHHHHH
EKLKFDAELVRERLEAKSYLHKGMTVVWKDETSSPPTSVTYKHDGGIAEYLTKVVTERNK
HHHCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCC
PLVPAGSAAFYHARDNGVRLEAALAWTEATDEHIRSYVNGIPTPLGGTHEAGLRSAVVKA
CCCCCCCCEEEEECCCCEEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VRNYIETHGIAPKGVTLTAEDIREGITAILSTYVVEPQFQGQTKGRLNNPEVSGQVDGVL
HHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
RPALEKWLNDNKSIAESVVARIVLAARAREASRAASQAVSRKTAVSHRLNLPGKLADCSS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TDPGLSELFIVEGDSAGGSAKQGRDRRTQAILPLRGKVLNAEQASTDKVTTNKELQDIVS
CCCCCEEEEEEECCCCCCCCCCCCCHHHHEECCCCCCEECCCCCCCCCCCCCHHHHHHHH
ALGCGIGSDFDISKLRYGRVFLLMDADSDGHHIATLLLTFFYRHLRPLIESGAIHIAQPP
HHCCCCCCCCCHHHEEECEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
LYRVDIGKETYWALDEPDRDRIIKEKTKGNAKPNIMRFKGLGEMTPDELKETTLDPKHRM
EEEEECCCCEEEECCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCE
SLRVTIDKPLETDRLINDLMGKDVSARFRFIMERASEVQDLDV
EEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]