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The map label for this gene is pksI [H]

Identifier: 108763516

GI number: 108763516

Start: 4797714

End: 4798457

Strand: Reverse

Name: pksI [H]

Synonym: MXAN_3939

Alternate gene names: 108763516

Gene position: 4798457-4797714 (Counterclockwise)

Preceding gene: 108757680

Following gene: 108760503

Centisome position: 52.5

GC content: 64.52

Gene sequence:

>744_bases
ATGGCGCCGGTGGTCACGCTCCATGAGGTGGAGGAGGGGGTGGCGCAGATCACCCTGGTGGATCGCGAGAACAAGAACAT
GTTCAGCGAGCAGCTCGTGCGCGAGCTCATCACCGTGTTCGGCAAGGTGAATGGAAACGAGCGCTACCGCGCGGTGGTGC
TCACCGGCTACGACACCTACTTCGCGCTCGGCGGGACCAAGGCCGGCCTGCTGTCCATCTGCGACGGCATCGGCTCCTTC
AACGTCACCAACTTCTACAGCCTCGCGCTGGAGTGCGACATCCCGGTGATTTCCGCCATGCAGGGACATGGCGTAGGCGG
CGGGTTCGCGATGGGGCTGTTCGCGGACTTCGTGGTCCTGAGCCGGGAGAGCGTCTACACGACGAACTTCATGCGCTACG
GCTTCACGCCGGGGATGGGCGCCACGTACATCGTGCCGAAGCGGCTGGGGTACTCGCTCGGGCATGAGCTCCTGCTCAAC
GCCAGGAACTACCGCGGCGCCGACCTGGAGAAGCGGGGCGTGCCTTTTCCGGTGTTGCCGCGCAAGGAAGTCTTGCCCCA
CGCCTACGAGATTGCGAGGGACCTGGCCGCGAAACCTCGGCTGTCGCTCGTGACGCTCAAGCGGCACCTGGTTCGCGACA
TCCGCCGAGAGCTTCCGGACGTCATCGAGCGTGAGCTGGAGATGCACGGCATCACCTTCCATCACGACGACGTGAGGAGG
CGCATCGAGCAGCTGTTCCTCTGA

Upstream 100 bases:

>100_bases
CCAATGAGGCGATGTTCTCCGACCGCGCCACGCTGGAAGCCATCCATCGCTACGTGGAGACAGGCCGACTCCCATGGGAA
TCATGACGGAAGGAACGCCA

Downstream 100 bases:

>100_bases
GGCGCGCCCCTATGTTGAACCTGATCAACAACCACGCACACGGTTATGTGGTCACGCCCGTGGTCCTGGCCTGCAACGAC
GCTGGCCTGTTCGAACTCCT

Product: polyketide biosynthesis enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MAPVVTLHEVEEGVAQITLVDRENKNMFSEQLVRELITVFGKVNGNERYRAVVLTGYDTYFALGGTKAGLLSICDGIGSF
NVTNFYSLALECDIPVISAMQGHGVGGGFAMGLFADFVVLSRESVYTTNFMRYGFTPGMGATYIVPKRLGYSLGHELLLN
ARNYRGADLEKRGVPFPVLPRKEVLPHAYEIARDLAAKPRLSLVTLKRHLVRDIRRELPDVIERELEMHGITFHHDDVRR
RIEQLFL

Sequences:

>Translated_247_residues
MAPVVTLHEVEEGVAQITLVDRENKNMFSEQLVRELITVFGKVNGNERYRAVVLTGYDTYFALGGTKAGLLSICDGIGSF
NVTNFYSLALECDIPVISAMQGHGVGGGFAMGLFADFVVLSRESVYTTNFMRYGFTPGMGATYIVPKRLGYSLGHELLLN
ARNYRGADLEKRGVPFPVLPRKEVLPHAYEIARDLAAKPRLSLVTLKRHLVRDIRRELPDVIERELEMHGITFHHDDVRR
RIEQLFL
>Mature_246_residues
APVVTLHEVEEGVAQITLVDRENKNMFSEQLVRELITVFGKVNGNERYRAVVLTGYDTYFALGGTKAGLLSICDGIGSFN
VTNFYSLALECDIPVISAMQGHGVGGGFAMGLFADFVVLSRESVYTTNFMRYGFTPGMGATYIVPKRLGYSLGHELLLNA
RNYRGADLEKRGVPFPVLPRKEVLPHAYEIARDLAAKPRLSLVTLKRHLVRDIRRELPDVIERELEMHGITFHHDDVRRR
IEQLFL

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. May have a role in the decarboxylation of the (S)-3-methylglutaryl group attached to pksL [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Escherichia coli, GI221142681, Length=210, Percent_Identity=27.1428571428571, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1788597, Length=210, Percent_Identity=22.8571428571429, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=231, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 27758; Mature: 27626

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPVVTLHEVEEGVAQITLVDRENKNMFSEQLVRELITVFGKVNGNERYRAVVLTGYDTY
CCCEEHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEE
FALGGTKAGLLSICDGIGSFNVTNFYSLALECDIPVISAMQGHGVGGGFAMGLFADFVVL
EEECCCCHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHHH
SRESVYTTNFMRYGFTPGMGATYIVPKRLGYSLGHELLLNARNYRGADLEKRGVPFPVLP
CCCCHHHHHHHEECCCCCCCCEEEEHHHHCHHHHHHHHHCCCCCCCCCCHHCCCCCCCCC
RKEVLPHAYEIARDLAAKPRLSLVTLKRHLVRDIRRELPDVIERELEMHGITFHHDDVRR
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHH
RIEQLFL
HHHHHCC
>Mature Secondary Structure 
APVVTLHEVEEGVAQITLVDRENKNMFSEQLVRELITVFGKVNGNERYRAVVLTGYDTY
CCEEHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEE
FALGGTKAGLLSICDGIGSFNVTNFYSLALECDIPVISAMQGHGVGGGFAMGLFADFVVL
EEECCCCHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHHH
SRESVYTTNFMRYGFTPGMGATYIVPKRLGYSLGHELLLNARNYRGADLEKRGVPFPVLP
CCCCHHHHHHHEECCCCCCCCEEEEHHHHCHHHHHHHHHCCCCCCCCCCHHCCCCCCCCC
RKEVLPHAYEIARDLAAKPRLSLVTLKRHLVRDIRRELPDVIERELEMHGITFHHDDVRR
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHH
RIEQLFL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]