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The map label for this gene is yfjR [H]

Identifier: 108763466

GI number: 108763466

Start: 5950614

End: 5951504

Strand: Reverse

Name: yfjR [H]

Synonym: MXAN_4748

Alternate gene names: 108763466

Gene position: 5951504-5950614 (Counterclockwise)

Preceding gene: 108758294

Following gene: 108761325

Centisome position: 65.12

GC content: 70.93

Gene sequence:

>891_bases
ATGAAGGTGGGATTCATTGGCCTGGGCAACATGGGACTGCCCATGGCGGCGAGCCTAGCGGCCGCGGGGCACGAGCTGAC
GGTGTGGAACCGCACCGAGTCCAAGGCCGCGCCGTTGAAAGAGAAGGGGGCGCGGGTCGCCCGCAGCCCCGCGGAGGCGG
CTCGAGGCGCGGAGGTCGTCTTCAGCATGCTGGCGGACGATGCGGCCGTGACATCCGCGGTGTTCGGCCAGGACGGCCTC
CTGGCGGGACTGGCGCCGGGCGCGGTGCACGTCTCGTCGAGCACGCTCTCCGTGGCGCTTTCCGAACGGTTGGCGGAGGC
GCATGCGAGCGCCGGCCAGCGGTATCTCGCCGCGCCGGTGTTCGGCCGCCCTACCGCCGCGGCGTCGAAGCAGCTGTGGG
TGGTGGCCGCCGGTCCGAAGCAGGACATGGACCGCTGCCGTCCGCTGCTGGAGACGCTGGGGCGCGGCCTCACCGTGCTG
GGTGACCGTGCCCCGGCGGCCAACGTCGTGAAGTTGTCGGGCAACTTCCTCATCGCGTCGATGATGGAGGCGCTCGCCGA
GTCCTTCGCCCTGACGCGCAAGTCCGGCATCGAGCCGAAGGTGTTCCTGGAGGTCTTCCAGTCCGTCTTCGCGCGCGCAC
CCATCTTCGAGGGGTACGCGCAGCTCATCGCCGAGGAGAAGTACAGCCCGGCGGGCTTCAAGATGCGGCTGGGCCTCAAG
GACGTGGAGCTCGTCCTGGGCGCCGCGCGCACCGCCGAGGTGCCCATGCCCCTGGCCAGCCTCGTGAAGGACCAGTTCCT
GGGAGGCGTCGCGCAGGGCCACGGCGATTTGGACTGGTCCGCGCTGGGGGCGCTCGTCGCCGAACGGGCCGGGCTGAAGC
AGGGCGACTGA

Upstream 100 bases:

>100_bases
CAGAATAGGTCGTCCCGTTCGCTCCTGGACGTGCGCGTGCTTTGCGGTGGTGGGGCTCCTGGGCGCGGATTATCCGGGCC
GTTTCCGGGAGGAGACGCGC

Downstream 100 bases:

>100_bases
GCGCCCGCGCTCCAGGTGCACGCCTGTCTGCCGTGCACCTGGAGCTTCAGGGATTGCGAGCAGCGCTTACTGCCGCGCGT
TCATCTCCGCTGCGGGGATG

Product: 3-hydroxyisobutyrate dehydrogenase family protein

Products: (R)-Glycerate; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKVGFIGLGNMGLPMAASLAAAGHELTVWNRTESKAAPLKEKGARVARSPAEAARGAEVVFSMLADDAAVTSAVFGQDGL
LAGLAPGAVHVSSSTLSVALSERLAEAHASAGQRYLAAPVFGRPTAAASKQLWVVAAGPKQDMDRCRPLLETLGRGLTVL
GDRAPAANVVKLSGNFLIASMMEALAESFALTRKSGIEPKVFLEVFQSVFARAPIFEGYAQLIAEEKYSPAGFKMRLGLK
DVELVLGAARTAEVPMPLASLVKDQFLGGVAQGHGDLDWSALGALVAERAGLKQGD

Sequences:

>Translated_296_residues
MKVGFIGLGNMGLPMAASLAAAGHELTVWNRTESKAAPLKEKGARVARSPAEAARGAEVVFSMLADDAAVTSAVFGQDGL
LAGLAPGAVHVSSSTLSVALSERLAEAHASAGQRYLAAPVFGRPTAAASKQLWVVAAGPKQDMDRCRPLLETLGRGLTVL
GDRAPAANVVKLSGNFLIASMMEALAESFALTRKSGIEPKVFLEVFQSVFARAPIFEGYAQLIAEEKYSPAGFKMRLGLK
DVELVLGAARTAEVPMPLASLVKDQFLGGVAQGHGDLDWSALGALVAERAGLKQGD
>Mature_296_residues
MKVGFIGLGNMGLPMAASLAAAGHELTVWNRTESKAAPLKEKGARVARSPAEAARGAEVVFSMLADDAAVTSAVFGQDGL
LAGLAPGAVHVSSSTLSVALSERLAEAHASAGQRYLAAPVFGRPTAAASKQLWVVAAGPKQDMDRCRPLLETLGRGLTVL
GDRAPAANVVKLSGNFLIASMMEALAESFALTRKSGIEPKVFLEVFQSVFARAPIFEGYAQLIAEEKYSPAGFKMRLGLK
DVELVLGAARTAEVPMPLASLVKDQFLGGVAQGHGDLDWSALGALVAERAGLKQGD

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=281, Percent_Identity=29.1814946619217, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI23308751, Length=293, Percent_Identity=27.3037542662116, Blast_Score=107, Evalue=9e-24,
Organism=Escherichia coli, GI1786719, Length=282, Percent_Identity=30.4964539007092, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI145693186, Length=282, Percent_Identity=29.4326241134752, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790315, Length=293, Percent_Identity=30.3754266211604, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789092, Length=285, Percent_Identity=29.4736842105263, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17557316, Length=266, Percent_Identity=28.1954887218045, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24655230, Length=295, Percent_Identity=29.1525423728814, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19922568, Length=295, Percent_Identity=29.1525423728814, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI28574115, Length=280, Percent_Identity=27.5, Blast_Score=114, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.60 [C]

Molecular weight: Translated: 30766; Mature: 30766

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGFIGLGNMGLPMAASLAAAGHELTVWNRTESKAAPLKEKGARVARSPAEAARGAEVV
CEEEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCCCCHHHCHHHHCCHHHHHHHHHHH
FSMLADDAAVTSAVFGQDGLLAGLAPGAVHVSSSTLSVALSERLAEAHASAGQRYLAAPV
HHHHHHHHHHHHHHHCCCCCEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEECCC
FGRPTAAASKQLWVVAAGPKQDMDRCRPLLETLGRGLTVLGDRAPAANVVKLSGNFLIAS
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCHHHH
MMEALAESFALTRKSGIEPKVFLEVFQSVFARAPIFEGYAQLIAEEKYSPAGFKMRLGLK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEECCH
DVELVLGAARTAEVPMPLASLVKDQFLGGVAQGHGDLDWSALGALVAERAGLKQGD
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVGFIGLGNMGLPMAASLAAAGHELTVWNRTESKAAPLKEKGARVARSPAEAARGAEVV
CEEEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCCCCHHHCHHHHCCHHHHHHHHHHH
FSMLADDAAVTSAVFGQDGLLAGLAPGAVHVSSSTLSVALSERLAEAHASAGQRYLAAPV
HHHHHHHHHHHHHHHCCCCCEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEECCC
FGRPTAAASKQLWVVAAGPKQDMDRCRPLLETLGRGLTVLGDRAPAANVVKLSGNFLIAS
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCHHHH
MMEALAESFALTRKSGIEPKVFLEVFQSVFARAPIFEGYAQLIAEEKYSPAGFKMRLGLK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEECCH
DVELVLGAARTAEVPMPLASLVKDQFLGGVAQGHGDLDWSALGALVAERAGLKQGD
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Hydroxy-3-oxopropanoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: 2-Hydroxy-3-oxopropanoate + H+ + Nicotinamide adenine dinucleotide - reduced --> (R)-Glycerate + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]