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The map label for this gene is ask [H]

Identifier: 108762767

GI number: 108762767

Start: 6109500

End: 6110693

Strand: Reverse

Name: ask [H]

Synonym: MXAN_4877

Alternate gene names: 108762767

Gene position: 6110693-6109500 (Counterclockwise)

Preceding gene: 108763357

Following gene: 108761192

Centisome position: 66.86

GC content: 70.77

Gene sequence:

>1194_bases
ATGCCAATCGTGGTCCAGAAGTACGGCGGCTCGTCGGTCGCCGGCGTGGAGAAGCTCCGCAAGGTCGCCCAGCGGGTGAA
GGACAAGCGCGAGGCGGGCTATCAGGTGGTGGTGGTGGTGAGCGCCATGGGCGACACCACGGATGAACTGCTGGCGCTGG
CGAAGGGCGTTTCGGTGGACCCGCCGCGCCGCGAACTGGACATGCTGCTCACGTGTGGCGAGCGCATCTCCATGGCGCTG
CTCTCCATGGCGCTCCAGGAGATGGATGTGCCGGCCATCAGCTTCACCGGAAGCCAGAGCGGCATCATCACCACGGACGC
GCACGCGCAGGCCCGCATCGTGGAGGTGCGGCCGTACCGCATCCACGACGAGCTGGCGCGCGGCAAGGTCGTCATCGTCG
CGGGCTACCAGGGTGTCTCCTTCAAGAAGGAAGTGACGACGCTGGGGCGCGGCGGCTCGGACACGACGGCGGTGGCGCTG
GCGGCGGCGCTGGACGCGGAGGCGTGTGAAATCTACTCGGACGTGGACGGCGTGTTCAGCGCGGACCCCCGGGTGGTGCC
GGACGCGCGCAAGCTGGAGTCGCTGAGCTACGACGAGATGCAGGAGTTGGCGAGCGCGGGCGCCACGGTGCTCAACGCGC
AGGCGGTGGAGTGGGCCAAGGCGCGCGGCATCACCATTCTCGCGCGCACCGCGCACGGCCAGGGAACGGGCACCGCGGTC
CAGGAGCTGGCCGTCCCCACGGACCACCGCCTCAAGGGCGTCACCGCCGACGCGGAGATGGCCGTGCTTTCCGCCTCGGA
GCCCATCCGCCTGGCGGAGCTGCTGGAATTCCTGGATGCGCGCGCCGTGCGCGGGCGGACGCTGGACTGCGAGGGCTCGG
TGGGTGGGGTACGCAGGACGGTCATCACCGTGCCCCTGGCGGACATCCACGGTGCGGACGCGCTGCGCCGGGAGCTGGCC
ACGCGCTTCGGTGACGCCGTGTCCTGGCGCGAGGACCTGGGCACCGTCACCTGCGTGGGCGTGGGGTTGAACGCCGACTG
GGCGCCGCTGCGCCGCGCGCTGGCCGCCGCCGAGGAGTTGGGGGCGCACGTCCACGCGGCCCATACGTCGCCTTTGCAAC
TGACGCTGCTGGTGGACAAAGCACACCTGAAGGCGCTGACGGCCCGGCTGCACCGAGAGCTCTTGGGAGGGTAG

Upstream 100 bases:

>100_bases
CTCCGGGTGTGGGCGGCGTTCTGCGGCTGGCCAGACTTCTGCAGGGAGGCCTACCAGTGTGGTTTTCCGGACACGCCGAA
CAAGGGTAGTGAGTCGAATC

Downstream 100 bases:

>100_bases
GCCTTTCCCATCCCTGGGCTTTTGTTCGGGTGCTCCATCCCAATCATTGCTCCAGGAGGGCACCCGCTTCCCTCCTGAGT
GTTCGTGGCTACCCTCCGGT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase; ASK [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MPIVVQKYGGSSVAGVEKLRKVAQRVKDKREAGYQVVVVVSAMGDTTDELLALAKGVSVDPPRRELDMLLTCGERISMAL
LSMALQEMDVPAISFTGSQSGIITTDAHAQARIVEVRPYRIHDELARGKVVIVAGYQGVSFKKEVTTLGRGGSDTTAVAL
AAALDAEACEIYSDVDGVFSADPRVVPDARKLESLSYDEMQELASAGATVLNAQAVEWAKARGITILARTAHGQGTGTAV
QELAVPTDHRLKGVTADAEMAVLSASEPIRLAELLEFLDARAVRGRTLDCEGSVGGVRRTVITVPLADIHGADALRRELA
TRFGDAVSWREDLGTVTCVGVGLNADWAPLRRALAAAEELGAHVHAAHTSPLQLTLLVDKAHLKALTARLHRELLGG

Sequences:

>Translated_397_residues
MPIVVQKYGGSSVAGVEKLRKVAQRVKDKREAGYQVVVVVSAMGDTTDELLALAKGVSVDPPRRELDMLLTCGERISMAL
LSMALQEMDVPAISFTGSQSGIITTDAHAQARIVEVRPYRIHDELARGKVVIVAGYQGVSFKKEVTTLGRGGSDTTAVAL
AAALDAEACEIYSDVDGVFSADPRVVPDARKLESLSYDEMQELASAGATVLNAQAVEWAKARGITILARTAHGQGTGTAV
QELAVPTDHRLKGVTADAEMAVLSASEPIRLAELLEFLDARAVRGRTLDCEGSVGGVRRTVITVPLADIHGADALRRELA
TRFGDAVSWREDLGTVTCVGVGLNADWAPLRRALAAAEELGAHVHAAHTSPLQLTLLVDKAHLKALTARLHRELLGG
>Mature_396_residues
PIVVQKYGGSSVAGVEKLRKVAQRVKDKREAGYQVVVVVSAMGDTTDELLALAKGVSVDPPRRELDMLLTCGERISMALL
SMALQEMDVPAISFTGSQSGIITTDAHAQARIVEVRPYRIHDELARGKVVIVAGYQGVSFKKEVTTLGRGGSDTTAVALA
AALDAEACEIYSDVDGVFSADPRVVPDARKLESLSYDEMQELASAGATVLNAQAVEWAKARGITILARTAHGQGTGTAVQ
ELAVPTDHRLKGVTADAEMAVLSASEPIRLAELLEFLDARAVRGRTLDCEGSVGGVRRTVITVPLADIHGADALRRELAT
RFGDAVSWREDLGTVTCVGVGLNADWAPLRRALAAAEELGAHVHAAHTSPLQLTLLVDKAHLKALTARLHRELLGG

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=140, Percent_Identity=40, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1790376, Length=324, Percent_Identity=25.9259259259259, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790455, Length=260, Percent_Identity=29.2307692307692, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320893, Length=187, Percent_Identity=26.7379679144385, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 42117; Mature: 41986

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIVVQKYGGSSVAGVEKLRKVAQRVKDKREAGYQVVVVVSAMGDTTDELLALAKGVSVD
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHCCCCCC
PPRRELDMLLTCGERISMALLSMALQEMDVPAISFTGSQSGIITTDAHAQARIVEVRPYR
CCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCCEEEEEECCEE
IHDELARGKVVIVAGYQGVSFKKEVTTLGRGGSDTTAVALAAALDAEACEIYSDVDGVFS
EHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCC
ADPRVVPDARKLESLSYDEMQELASAGATVLNAQAVEWAKARGITILARTAHGQGTGTAV
CCCCCCCCHHHHHCCCHHHHHHHHHCCCCEEEHHHHHHHHHCCEEEEEEECCCCCCCHHH
QELAVPTDHRLKGVTADAEMAVLSASEPIRLAELLEFLDARAVRGRTLDCEGSVGGVRRT
HHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEE
VITVPLADIHGADALRRELATRFGDAVSWREDLGTVTCVGVGLNADWAPLRRALAAAEEL
EEEEECHHCCCHHHHHHHHHHHHCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHH
GAHVHAAHTSPLQLTLLVDKAHLKALTARLHRELLGG
CCEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PIVVQKYGGSSVAGVEKLRKVAQRVKDKREAGYQVVVVVSAMGDTTDELLALAKGVSVD
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHCCCCCC
PPRRELDMLLTCGERISMALLSMALQEMDVPAISFTGSQSGIITTDAHAQARIVEVRPYR
CCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCCEEEEEECCEE
IHDELARGKVVIVAGYQGVSFKKEVTTLGRGGSDTTAVALAAALDAEACEIYSDVDGVFS
EHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCC
ADPRVVPDARKLESLSYDEMQELASAGATVLNAQAVEWAKARGITILARTAHGQGTGTAV
CCCCCCCCHHHHHCCCHHHHHHHHHCCCCEEEHHHHHHHHHCCEEEEEEECCCCCCCHHH
QELAVPTDHRLKGVTADAEMAVLSASEPIRLAELLEFLDARAVRGRTLDCEGSVGGVRRT
HHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEE
VITVPLADIHGADALRRELATRFGDAVSWREDLGTVTCVGVGLNADWAPLRRALAAAEEL
EEEEECHHCCCHHHHHHHHHHHHCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHH
GAHVHAAHTSPLQLTLLVDKAHLKALTARLHRELLGG
CCEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7910936 [H]