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The map label for this gene is thiO/thiG [H]

Identifier: 108762673

GI number: 108762673

Start: 5674520

End: 5675629

Strand: Reverse

Name: thiO/thiG [H]

Synonym: MXAN_4550

Alternate gene names: 108762673

Gene position: 5675629-5674520 (Counterclockwise)

Preceding gene: 108761474

Following gene: 108759711

Centisome position: 62.1

GC content: 72.34

Gene sequence:

>1110_bases
ATGAAGCCTGGAGCCGACATCTTGATTCTCGGAGCCGGTGTCGTGGGCCTTTCGGCCGCGCGCCGGCTTTCTGCTTTGGG
CGCGAGCGTCACGGTGCTGGACGCCGTGGATCCGGGAGGCCGGGGCTCGCGCGCCGCCGCCGGAGTCGCCATTCCGTCCG
TGCGGTTGTTCGATGACCCGGTGATGCTGGCGTTCGCCCGGGCAGGCCGCGGCACGTTGGCGGAGGACCTGGCCTCGCTT
CCGGGTGGCGACCAGCTTCGCCGAGGCAACGGCATCCTCCGCGTCGTCGCGGACGCGAAGGCGCGCGATGCGCTGGCGGC
GAAAGCGGTGGCGGACGCGGAAGGACTGGGCACCTGGCGGGACGCCGCGAGCCTGGTGTCGCTCGAGCCCGCGCTGGAAG
GTACGCCGCTGTTCGGTGCCTTCGAGACGGACCAGGGCCACCTCGTGGACACGGATGGCTACGTCGACGCGCTCCTGAAG
GCCGCTGCCCACGTGGGCGTGCGGTTGCGGCTGGGCGTGGCCGCGCGCTCCGTTTCCGAATCCTCCACGGGGGTCGAGGT
CCAGACGGAAGGGGAGACGCTCCGGGCGGACCAGTTGCTCGTGTGCGCGGGGCCCTGGTCGGCGGGCGTGGAGGGGTTGC
CTTCGCTGCCGTTGAAGCCCTTGCGTGGGCAGATGCTGGTGGTGCACCAGCCGGGATTGCACCTGACGCGCGTCGTCTCA
GGGCCTTCGTACCTGGCGCCGTGGCGCTCGGGCGAAATCGTCGTGGGGGCGACGGAGGAGGACGCGGGCTTCGTGGAGCA
GGTGACGCCCGCGGGCCTGCTGCACCTCAGTGCCACCGTCGCGAAGCTGGCGCCGCGACTGCGTGACGCCCGGTTCGTCC
GCGCATGGGCGGGGCTTCGGGCCGCGACGCCGGATGGCCACCCGTACATCGGCCGTCATCCCGGCACCCGGCGCACCTAC
GTCGCCACGGGGCTGGGTGGCCAGGGCATCCTGACCGGTGCGCATACCGGTGCCTTGCTCGCGGAGCTCGTGGCGCACGG
CCATTGCGCCGCCGCCGAGCCGTTCTCGCCCGCGCGTATCACCGACGGTTCTCCGCTCGCAACGCGCTGA

Upstream 100 bases:

>100_bases
GGCTACACCCAGTTGGACGCGGTGGTGAAGCGCGCCGAGCGGCACGGCTTCATCTACGTGGATGGTGGCTTTCACGTCAT
CGCCAAGCGTCTCAGGTGAA

Downstream 100 bases:

>100_bases
GCAGGTGCTCGGCGCTCACGGATGCGCTGATGCGCGCCGGCGGGGCACACGCCCGGGGAAGGGCGCCCGCGCCCCAGCGG
GACGTCAGTACAGGTTCACC

Product: FAD-dependent oxidoreductase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKPGADILILGAGVVGLSAARRLSALGASVTVLDAVDPGGRGSRAAAGVAIPSVRLFDDPVMLAFARAGRGTLAEDLASL
PGGDQLRRGNGILRVVADAKARDALAAKAVADAEGLGTWRDAASLVSLEPALEGTPLFGAFETDQGHLVDTDGYVDALLK
AAAHVGVRLRLGVAARSVSESSTGVEVQTEGETLRADQLLVCAGPWSAGVEGLPSLPLKPLRGQMLVVHQPGLHLTRVVS
GPSYLAPWRSGEIVVGATEEDAGFVEQVTPAGLLHLSATVAKLAPRLRDARFVRAWAGLRAATPDGHPYIGRHPGTRRTY
VATGLGGQGILTGAHTGALLAELVAHGHCAAAEPFSPARITDGSPLATR

Sequences:

>Translated_369_residues
MKPGADILILGAGVVGLSAARRLSALGASVTVLDAVDPGGRGSRAAAGVAIPSVRLFDDPVMLAFARAGRGTLAEDLASL
PGGDQLRRGNGILRVVADAKARDALAAKAVADAEGLGTWRDAASLVSLEPALEGTPLFGAFETDQGHLVDTDGYVDALLK
AAAHVGVRLRLGVAARSVSESSTGVEVQTEGETLRADQLLVCAGPWSAGVEGLPSLPLKPLRGQMLVVHQPGLHLTRVVS
GPSYLAPWRSGEIVVGATEEDAGFVEQVTPAGLLHLSATVAKLAPRLRDARFVRAWAGLRAATPDGHPYIGRHPGTRRTY
VATGLGGQGILTGAHTGALLAELVAHGHCAAAEPFSPARITDGSPLATR
>Mature_369_residues
MKPGADILILGAGVVGLSAARRLSALGASVTVLDAVDPGGRGSRAAAGVAIPSVRLFDDPVMLAFARAGRGTLAEDLASL
PGGDQLRRGNGILRVVADAKARDALAAKAVADAEGLGTWRDAASLVSLEPALEGTPLFGAFETDQGHLVDTDGYVDALLK
AAAHVGVRLRLGVAARSVSESSTGVEVQTEGETLRADQLLVCAGPWSAGVEGLPSLPLKPLRGQMLVVHQPGLHLTRVVS
GPSYLAPWRSGEIVVGATEEDAGFVEQVTPAGLLHLSATVAKLAPRLRDARFVRAWAGLRAATPDGHPYIGRHPGTRRTY
VATGLGGQGILTGAHTGALLAELVAHGHCAAAEPFSPARITDGSPLATR

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=398, Percent_Identity=22.6130653266332, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 37765; Mature: 37765

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00962 RIBOSOMAL_S2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPGADILILGAGVVGLSAARRLSALGASVTVLDAVDPGGRGSRAAAGVAIPSVRLFDDP
CCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEECCCEEEECCC
VMLAFARAGRGTLAEDLASLPGGDQLRRGNGILRVVADAKARDALAAKAVADAEGLGTWR
EEEEEECCCCCHHHHHHHHCCCCCCEECCCCEEEEEECCCHHHHHHHHHHHCCCCCCCHH
DAASLVSLEPALEGTPLFGAFETDQGHLVDTDGYVDALLKAAAHVGVRLRLGVAARSVSE
HHHHHEEECCCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHCCEEEEEEHHHHCCCC
SSTGVEVQTEGETLRADQLLVCAGPWSAGVEGLPSLPLKPLRGQMLVVHQPGLHLTRVVS
CCCCEEEEECCCEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEC
GPSYLAPWRSGEIVVGATEEDAGFVEQVTPAGLLHLSATVAKLAPRLRDARFVRAWAGLR
CCCEECCCCCCCEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AATPDGHPYIGRHPGTRRTYVATGLGGQGILTGAHTGALLAELVAHGHCAAAEPFSPARI
CCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCEE
TDGSPLATR
CCCCCCCCC
>Mature Secondary Structure
MKPGADILILGAGVVGLSAARRLSALGASVTVLDAVDPGGRGSRAAAGVAIPSVRLFDDP
CCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEECCCEEEECCC
VMLAFARAGRGTLAEDLASLPGGDQLRRGNGILRVVADAKARDALAAKAVADAEGLGTWR
EEEEEECCCCCHHHHHHHHCCCCCCEECCCCEEEEEECCCHHHHHHHHHHHCCCCCCCHH
DAASLVSLEPALEGTPLFGAFETDQGHLVDTDGYVDALLKAAAHVGVRLRLGVAARSVSE
HHHHHEEECCCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHCCEEEEEEHHHHCCCC
SSTGVEVQTEGETLRADQLLVCAGPWSAGVEGLPSLPLKPLRGQMLVVHQPGLHLTRVVS
CCCCEEEEECCCEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEC
GPSYLAPWRSGEIVVGATEEDAGFVEQVTPAGLLHLSATVAKLAPRLRDARFVRAWAGLR
CCCEECCCCCCCEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AATPDGHPYIGRHPGTRRTYVATGLGGQGILTGAHTGALLAELVAHGHCAAAEPFSPARI
CCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCEE
TDGSPLATR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]