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The map label for this gene is glgX [H]

Identifier: 108762557

GI number: 108762557

Start: 493937

End: 496063

Strand: Reverse

Name: glgX [H]

Synonym: MXAN_0434

Alternate gene names: 108762557

Gene position: 496063-493937 (Counterclockwise)

Preceding gene: 108758021

Following gene: 108758169

Centisome position: 5.43

GC content: 67.47

Gene sequence:

>2127_bases
ATGAGTCGGGAAGTCTGGCCAGGCAAGCCCTGGCCTCGCGGCGCCACCTTCGATGGTTCAGGGGTCAACTTCGCCATCTA
CTCCCAGGTCGCGACCCGCGTGGAGGTCTGCCTGTTCGACCCCGCGGACCCGGCGCGCGAAATCGAACGGTTCGGCCTGC
CGGAGTCCACGGACTTCGTGCACCACGGCTACGTGCCGGGCCTGGAGCCAGGCACGCTCTACGGTCTGCGCGTTCATGGC
CCGTACGAGCCCACCAAGGGCCACCGCTGCAATCCGCACAAGCTGCTGGTGGACCCCTACGCCAAGGCGCTCTACGGCGA
CGTGGACTGGCGCCAGCCGGTGTTCGGCTATCCACTGGGGCACGAGCAGCAGGACCTGGCGCGAGACGAACGCGACAGCG
CGGCGGGCGTGCCCAAGTCCGTGGTGGTGAGCGACTACTTCGACTGGGGCAATGACCGGCGCCCGGACATCAACTGGCGT
GAGACGGTGCTGTACGAGGCTCACGTGCGCGGCCTCACCATGCGCCACCCCGGCGTGCCCGAACACCTGCGTGGCACCTA
CGCGGGCCTGGCCTGCCCGCCCGTCATCGAGCATTTGCTGAAGCTGGGCGTGACGTCGGTGGAGTTGCTGCCGGTGCATG
CCTTCGCGGACGACTCGTTCCTCGACGACAAGAAGCTGTCCAACTTCTGGGGCTACAACACGCTGGGCTACTTCGCGCCG
GAGCAGTACTACGCCAGTCGCAAGACGCCCGGCGCCGCCGTCGCCGAGTTCAAGGCGATGGTGAGGGACCTGCACGCCGC
GGGCATCGAGGTCATCCTCGACGTCGTCTACAACCACACCTGCGAGGGCAACCACCTGGGGCCCACGCTGTCGCTCAAGG
GCATCGACAACGCGAGCTACTACTGGCTGATGCCGGACGGGCGGCACTACCTGGACTTCACCGGGTGCGGCAACAGCATC
AACGCGTCCAACCCGCAGGCGGCGCGGCTCATCATCGACAGCCTCCGCTACTGGGTGACGGAGATGCACGTGGACGGGTT
CCGCTTCGACCTCGCCACGGTGCTGGGCCGCACGGGTGAAGGCGCGTTCGACCGCGACGCGGCGCTGTTCCAGATTCTCC
ACCAGGACCCGGTGCTCGGCCGCGTGAAACTCATCGCCGAGCCCTGGGACGTGGGCCTCGGCGGCTACCAGGTGGGCGGA
TTCCCTCCGCCCTGGCGCGAGTGGAACGGCAAGTACCGGGACGCGCTGCGCCGCTACTGGAAGGGCGATGAGAACCTCGC
CAGTGAGATGGGCTACCGAATCACGGGCAACGCGGACCTGTACGCGGAGGCGCGCCGGCGGCCCCAGGCGAGCATCAACT
TCGTCACCGCGCATGACGGCTTCACGCTGCACGACCTGGTGACGTACAGCCACAAGCACAACGAGGCCAACGGCGAGCAC
AACCGTGACGGCGCGGACGACAACCAGTCGTGGAACTGCGGCGTGGAGGGTGAGACGGACAAAGCGGACGTCATCGCCCT
GCGCGAGCGGCAGAAGCGCAACCTGCTGGCCTCGCTCTTCCTGTCCACCGGCATCCCGATGATTGTCGCGGGTGACGAGA
TGGGCCGCACGCAGGGCGGCAACAACAACGCGTACTGCCAGGACAACGAGCTGTCGTGGGTGGATTGGAACCTGGATGAG
CGGCGGCGGAAGCTGCTGGCGTTCACGCGCAAGCTCATCCACTTCCGCCACCGCCAGCCGGTGCTCCAGCGCCGCCGCTT
CTTCAAGGGTCAGCACCTGTGGGACTCCGAGCACAAGGACCTGACGTGGTTCCGGCCGGATGGCCCGGAGATGAAGGCGG
AGGACTGGGAGAAGCCCTTCGTGCGCTCGCTGGCATTCCTCCTGGGCGGCGACGCCATCCCCACGCCGGACGAGCGCGGG
CAGCGCATCATCGGTGACGCGCTGCTGATACTGCTCAACTCGCACCATGAGCCGGTGACGTACAAGCTGCCGCCTGCCGC
GCAGAGCCAGCGATGGGAGCTGGAGCTCTGCACCACCGACGACAACCGGGGACCGGAGCCGGTGAAAGGTGAGACGTTCG
AGCTCATCGGCCGCTCGCTCGCGGTGTTCCGGCAGGTCGCGGACTGA

Upstream 100 bases:

>100_bases
CTGGCGCTCACTGGACCACGCTGTCGCCGCGTGACGCTTGCCAGCCCAGAGGTGCCATCCCACATTGAGCAGGCCTTTCC
ATCAGAATCGAGAGCCTGCA

Downstream 100 bases:

>100_bases
GGCAGATGCCGCATGCTAGGGTTCCCCGCCGAGGCCTTCCCCGGCGGGTGCGGCATGCCGGTGCCTAAGCGAAGCAGGTC
CGCCGCCAGGGCGCTCGCAC

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 708; Mature: 707

Protein sequence:

>708_residues
MSREVWPGKPWPRGATFDGSGVNFAIYSQVATRVEVCLFDPADPAREIERFGLPESTDFVHHGYVPGLEPGTLYGLRVHG
PYEPTKGHRCNPHKLLVDPYAKALYGDVDWRQPVFGYPLGHEQQDLARDERDSAAGVPKSVVVSDYFDWGNDRRPDINWR
ETVLYEAHVRGLTMRHPGVPEHLRGTYAGLACPPVIEHLLKLGVTSVELLPVHAFADDSFLDDKKLSNFWGYNTLGYFAP
EQYYASRKTPGAAVAEFKAMVRDLHAAGIEVILDVVYNHTCEGNHLGPTLSLKGIDNASYYWLMPDGRHYLDFTGCGNSI
NASNPQAARLIIDSLRYWVTEMHVDGFRFDLATVLGRTGEGAFDRDAALFQILHQDPVLGRVKLIAEPWDVGLGGYQVGG
FPPPWREWNGKYRDALRRYWKGDENLASEMGYRITGNADLYAEARRRPQASINFVTAHDGFTLHDLVTYSHKHNEANGEH
NRDGADDNQSWNCGVEGETDKADVIALRERQKRNLLASLFLSTGIPMIVAGDEMGRTQGGNNNAYCQDNELSWVDWNLDE
RRRKLLAFTRKLIHFRHRQPVLQRRRFFKGQHLWDSEHKDLTWFRPDGPEMKAEDWEKPFVRSLAFLLGGDAIPTPDERG
QRIIGDALLILLNSHHEPVTYKLPPAAQSQRWELELCTTDDNRGPEPVKGETFELIGRSLAVFRQVAD

Sequences:

>Translated_708_residues
MSREVWPGKPWPRGATFDGSGVNFAIYSQVATRVEVCLFDPADPAREIERFGLPESTDFVHHGYVPGLEPGTLYGLRVHG
PYEPTKGHRCNPHKLLVDPYAKALYGDVDWRQPVFGYPLGHEQQDLARDERDSAAGVPKSVVVSDYFDWGNDRRPDINWR
ETVLYEAHVRGLTMRHPGVPEHLRGTYAGLACPPVIEHLLKLGVTSVELLPVHAFADDSFLDDKKLSNFWGYNTLGYFAP
EQYYASRKTPGAAVAEFKAMVRDLHAAGIEVILDVVYNHTCEGNHLGPTLSLKGIDNASYYWLMPDGRHYLDFTGCGNSI
NASNPQAARLIIDSLRYWVTEMHVDGFRFDLATVLGRTGEGAFDRDAALFQILHQDPVLGRVKLIAEPWDVGLGGYQVGG
FPPPWREWNGKYRDALRRYWKGDENLASEMGYRITGNADLYAEARRRPQASINFVTAHDGFTLHDLVTYSHKHNEANGEH
NRDGADDNQSWNCGVEGETDKADVIALRERQKRNLLASLFLSTGIPMIVAGDEMGRTQGGNNNAYCQDNELSWVDWNLDE
RRRKLLAFTRKLIHFRHRQPVLQRRRFFKGQHLWDSEHKDLTWFRPDGPEMKAEDWEKPFVRSLAFLLGGDAIPTPDERG
QRIIGDALLILLNSHHEPVTYKLPPAAQSQRWELELCTTDDNRGPEPVKGETFELIGRSLAVFRQVAD
>Mature_707_residues
SREVWPGKPWPRGATFDGSGVNFAIYSQVATRVEVCLFDPADPAREIERFGLPESTDFVHHGYVPGLEPGTLYGLRVHGP
YEPTKGHRCNPHKLLVDPYAKALYGDVDWRQPVFGYPLGHEQQDLARDERDSAAGVPKSVVVSDYFDWGNDRRPDINWRE
TVLYEAHVRGLTMRHPGVPEHLRGTYAGLACPPVIEHLLKLGVTSVELLPVHAFADDSFLDDKKLSNFWGYNTLGYFAPE
QYYASRKTPGAAVAEFKAMVRDLHAAGIEVILDVVYNHTCEGNHLGPTLSLKGIDNASYYWLMPDGRHYLDFTGCGNSIN
ASNPQAARLIIDSLRYWVTEMHVDGFRFDLATVLGRTGEGAFDRDAALFQILHQDPVLGRVKLIAEPWDVGLGGYQVGGF
PPPWREWNGKYRDALRRYWKGDENLASEMGYRITGNADLYAEARRRPQASINFVTAHDGFTLHDLVTYSHKHNEANGEHN
RDGADDNQSWNCGVEGETDKADVIALRERQKRNLLASLFLSTGIPMIVAGDEMGRTQGGNNNAYCQDNELSWVDWNLDER
RRKLLAFTRKLIHFRHRQPVLQRRRFFKGQHLWDSEHKDLTWFRPDGPEMKAEDWEKPFVRSLAFLLGGDAIPTPDERGQ
RIIGDALLILLNSHHEPVTYKLPPAAQSQRWELELCTTDDNRGPEPVKGETFELIGRSLAVFRQVAD

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=657, Percent_Identity=45.0532724505327, Blast_Score=542, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 80178; Mature: 80047

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREVWPGKPWPRGATFDGSGVNFAIYSQVATRVEVCLFDPADPAREIERFGLPESTDFV
CCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHEEEEEEECCCCHHHHHHHCCCCCCCCCE
HHGYVPGLEPGTLYGLRVHGPYEPTKGHRCNPHKLLVDPYAKALYGDVDWRQPVFGYPLG
ECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCCCCCCCC
HEQQDLARDERDSAAGVPKSVVVSDYFDWGNDRRPDINWRETVLYEAHVRGLTMRHPGVP
CCHHHHHHHHCHHHCCCCCCEEEEHHHCCCCCCCCCCCHHHHEEEEHHHCEEEECCCCCC
EHLRGTYAGLACPPVIEHLLKLGVTSVELLPVHAFADDSFLDDKKLSNFWGYNTLGYFAP
HHHCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCC
EQYYASRKTPGAAVAEFKAMVRDLHAAGIEVILDVVYNHTCEGNHLGPTLSLKGIDNASY
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEE
YWLMPDGRHYLDFTGCGNSINASNPQAARLIIDSLRYWVTEMHVDGFRFDLATVLGRTGE
EEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
GAFDRDAALFQILHQDPVLGRVKLIAEPWDVGLGGYQVGGFPPPWREWNGKYRDALRRYW
CCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEECCCCCCCHHHCCCHHHHHHHHHH
KGDENLASEMGYRITGNADLYAEARRRPQASINFVTAHDGFTLHDLVTYSHKHNEANGEH
CCCHHHHHHCCEEEECCCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
NRDGADDNQSWNCGVEGETDKADVIALRERQKRNLLASLFLSTGIPMIVAGDEMGRTQGG
CCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCC
NNNAYCQDNELSWVDWNLDERRRKLLAFTRKLIHFRHRQPVLQRRRFFKGQHLWDSEHKD
CCCCEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCC
LTWFRPDGPEMKAEDWEKPFVRSLAFLLGGDAIPTPDERGQRIIGDALLILLNSHHEPVT
CEEECCCCCCCCCHHCCHHHHHHHHHHHCCCCCCCCCHHCHHHHHHHHHHHCCCCCCCEE
YKLPPAAQSQRWELELCTTDDNRGPEPVKGETFELIGRSLAVFRQVAD
EECCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SREVWPGKPWPRGATFDGSGVNFAIYSQVATRVEVCLFDPADPAREIERFGLPESTDFV
CCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHEEEEEEECCCCHHHHHHHCCCCCCCCCE
HHGYVPGLEPGTLYGLRVHGPYEPTKGHRCNPHKLLVDPYAKALYGDVDWRQPVFGYPLG
ECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCCCCCCCC
HEQQDLARDERDSAAGVPKSVVVSDYFDWGNDRRPDINWRETVLYEAHVRGLTMRHPGVP
CCHHHHHHHHCHHHCCCCCCEEEEHHHCCCCCCCCCCCHHHHEEEEHHHCEEEECCCCCC
EHLRGTYAGLACPPVIEHLLKLGVTSVELLPVHAFADDSFLDDKKLSNFWGYNTLGYFAP
HHHCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCC
EQYYASRKTPGAAVAEFKAMVRDLHAAGIEVILDVVYNHTCEGNHLGPTLSLKGIDNASY
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEE
YWLMPDGRHYLDFTGCGNSINASNPQAARLIIDSLRYWVTEMHVDGFRFDLATVLGRTGE
EEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
GAFDRDAALFQILHQDPVLGRVKLIAEPWDVGLGGYQVGGFPPPWREWNGKYRDALRRYW
CCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEECCCCCCCHHHCCCHHHHHHHHHH
KGDENLASEMGYRITGNADLYAEARRRPQASINFVTAHDGFTLHDLVTYSHKHNEANGEH
CCCHHHHHHCCEEEECCCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
NRDGADDNQSWNCGVEGETDKADVIALRERQKRNLLASLFLSTGIPMIVAGDEMGRTQGG
CCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCC
NNNAYCQDNELSWVDWNLDERRRKLLAFTRKLIHFRHRQPVLQRRRFFKGQHLWDSEHKD
CCCCEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCC
LTWFRPDGPEMKAEDWEKPFVRSLAFLLGGDAIPTPDERGQRIIGDALLILLNSHHEPVT
CEEECCCCCCCCCHHCCHHHHHHHHHHHCCCCCCCCCHHCHHHHHHHHHHHCCCCCCCEE
YKLPPAAQSQRWELELCTTDDNRGPEPVKGETFELIGRSLAVFRQVAD
EECCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]