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The map label for this gene is pccA [H]

Identifier: 108762533

GI number: 108762533

Start: 1293166

End: 1294683

Strand: Reverse

Name: pccA [H]

Synonym: MXAN_1111

Alternate gene names: 108762533

Gene position: 1294683-1293166 (Counterclockwise)

Preceding gene: 108757674

Following gene: 108761465

Centisome position: 14.17

GC content: 68.05

Gene sequence:

>1518_bases
ATGCCCAAGATCCGCAAAGTGCTCGTCGCCAATCGCGGCGAGATCGCCATCCGGGTGATGCGCACGTGCAAGGAACTCGG
CATCGCCACGGTGGCGGTGTACTCGGAGGCGGACCGCTCCGCCCTGCATGTGCGCACGGCCGACCAGGCCATCTTCGTGG
GGCCCCCGCCTTCGCGTGAGAGCTACCTGGTGCAGCAGCGCATCCTCGACGCCGCGAAGCAGGCTGGCGCGGATGCCATC
CACCCCGGCTACGGCTTCCTCTCCGAAAACGCCGCCTTCGTCCGCGCCTGTGAGGCCGAAGGCCTGACGTTCATCGGGCC
GCCCGCCTCCGCCATGGACTCCATGGGCGAGAAGACGCGTGCCCGCGCGAACATGATCAAGGCGGGCGTGCCCGTGGTGC
CCGGTACCACCGAGCCCATCCCCTCCGTGGAGGAAGCGCGCGCCTACGTGGAGAAGATTGGCTTCCCCGTCATGCTCAAG
GCCGCGGGCGGCGGCGGTGGCAAGGGCATGCGCAAGGTGGAGCGCATGGAGGACTTCGAGTCCTCGTGGCGCGCCGCCAA
GAGCGAGGCCCTCAACGCCTTCGGCAACGACGCCGTCTACATCGAGAAGTACCTGGAGAAGCCACACCACGTGGAGATCC
AGGTCTTCGCGGACCAGTACGGCAACACCATCCACCTGAACGAGCGCGAGTGCTCGGCGCAGCGCCGGCACCAGAAGGTG
GTGGAGGAGACGCCCAGCCCCATCCTCACGCCGGAGCTGCGCGCGAAGATGGGCGAGGTCGCGGTGAAGGCGGCCAAGGC
CGTCAACTACGTGGGCGCCGGGACGGTGGAGTTCCTGGTGGACGTGCACCGCAACTTCTACTTCCTGGAGATGAACACCC
GCCTCCAGGTGGAGCACCCGGTGACGGAGTGGGTGACGGGCCTGGACCTGGTGGCCATGCAGATCAAGGCCGCCGAGGGC
GAGAAGCTCGGCCTCACCGAAGCCCCCACGCCGCGCGGTCATTCGATTGAGGTCCGCGTGTACGCGGAGGACCCGAGCCG
CAACTTCATGCCCAGCCCGGGCAAGATTACGTACCTGCGCGTGCCGGGTGGACCGAACGTGCGTGACGATTCGGGCGTGT
TCCCCGGGTACACGGTGCCCAACGTCTACGACCCGATGATTTCCAAGCTGTCCGTGTGGGCCCCCACGCGGCGCGAGGCC
ATCGCCCGCACGCAGCGCGCGCTGTCCGAGTACGTGGTGAAGGGCATCACCACCAACATCCGCTACCTGAAGGCGATTCT
GGCGCACCCCGAGTTCATCGAGGGCGACTACGACACCAGCTTCCTCACCCGCCAGCACGATGCGCTGCTGGGCGCGGAGG
ACCCGAAGCTGAGCGAGATGGCGCTGCTGGCCAGCGTGGTGCACACGTACCAGAAGGACCAGAAGCGCGCGAAGACCCTG
CCTGCTGCCAGTGGCGGCCAGGGCGGTGGCAACGGCGGCGTCTCCCCGTGGAAGCTGGCGCTGCGCGCGCGCCGCTAA

Upstream 100 bases:

>100_bases
TGGAGGCCAAGCTGACACAGGCTCGCGCTTACATGGAGCAGTACCTGAACACCCGGAAGTAGAGGCGGCAAACAGGGTCG
CCCCGTGCTAGACGGGCCCC

Downstream 100 bases:

>100_bases
CCCCCAGCTCGGAAGGACTCCCATGCGTTACTTCACGAAGCAGCAGGGCCAGAAGGAAGCGGTCCCGGTGGACCTGGAGC
CCCTGGGGAACGACCAGTTC

Product: propionyl-CoA carboxylase subunit alpha

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MPKIRKVLVANRGEIAIRVMRTCKELGIATVAVYSEADRSALHVRTADQAIFVGPPPSRESYLVQQRILDAAKQAGADAI
HPGYGFLSENAAFVRACEAEGLTFIGPPASAMDSMGEKTRARANMIKAGVPVVPGTTEPIPSVEEARAYVEKIGFPVMLK
AAGGGGGKGMRKVERMEDFESSWRAAKSEALNAFGNDAVYIEKYLEKPHHVEIQVFADQYGNTIHLNERECSAQRRHQKV
VEETPSPILTPELRAKMGEVAVKAAKAVNYVGAGTVEFLVDVHRNFYFLEMNTRLQVEHPVTEWVTGLDLVAMQIKAAEG
EKLGLTEAPTPRGHSIEVRVYAEDPSRNFMPSPGKITYLRVPGGPNVRDDSGVFPGYTVPNVYDPMISKLSVWAPTRREA
IARTQRALSEYVVKGITTNIRYLKAILAHPEFIEGDYDTSFLTRQHDALLGAEDPKLSEMALLASVVHTYQKDQKRAKTL
PAASGGQGGGNGGVSPWKLALRARR

Sequences:

>Translated_505_residues
MPKIRKVLVANRGEIAIRVMRTCKELGIATVAVYSEADRSALHVRTADQAIFVGPPPSRESYLVQQRILDAAKQAGADAI
HPGYGFLSENAAFVRACEAEGLTFIGPPASAMDSMGEKTRARANMIKAGVPVVPGTTEPIPSVEEARAYVEKIGFPVMLK
AAGGGGGKGMRKVERMEDFESSWRAAKSEALNAFGNDAVYIEKYLEKPHHVEIQVFADQYGNTIHLNERECSAQRRHQKV
VEETPSPILTPELRAKMGEVAVKAAKAVNYVGAGTVEFLVDVHRNFYFLEMNTRLQVEHPVTEWVTGLDLVAMQIKAAEG
EKLGLTEAPTPRGHSIEVRVYAEDPSRNFMPSPGKITYLRVPGGPNVRDDSGVFPGYTVPNVYDPMISKLSVWAPTRREA
IARTQRALSEYVVKGITTNIRYLKAILAHPEFIEGDYDTSFLTRQHDALLGAEDPKLSEMALLASVVHTYQKDQKRAKTL
PAASGGQGGGNGGVSPWKLALRARR
>Mature_504_residues
PKIRKVLVANRGEIAIRVMRTCKELGIATVAVYSEADRSALHVRTADQAIFVGPPPSRESYLVQQRILDAAKQAGADAIH
PGYGFLSENAAFVRACEAEGLTFIGPPASAMDSMGEKTRARANMIKAGVPVVPGTTEPIPSVEEARAYVEKIGFPVMLKA
AGGGGGKGMRKVERMEDFESSWRAAKSEALNAFGNDAVYIEKYLEKPHHVEIQVFADQYGNTIHLNERECSAQRRHQKVV
EETPSPILTPELRAKMGEVAVKAAKAVNYVGAGTVEFLVDVHRNFYFLEMNTRLQVEHPVTEWVTGLDLVAMQIKAAEGE
KLGLTEAPTPRGHSIEVRVYAEDPSRNFMPSPGKITYLRVPGGPNVRDDSGVFPGYTVPNVYDPMISKLSVWAPTRREAI
ARTQRALSEYVVKGITTNIRYLKAILAHPEFIEGDYDTSFLTRQHDALLGAEDPKLSEMALLASVVHTYQKDQKRAKTLP
AASGGQGGGNGGVSPWKLALRARR

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI116805327, Length=449, Percent_Identity=52.3385300668151, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI65506442, Length=477, Percent_Identity=48.6373165618449, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI189095269, Length=477, Percent_Identity=48.6373165618449, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI295821183, Length=477, Percent_Identity=48.6373165618449, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI106049528, Length=463, Percent_Identity=47.5161987041037, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI106049295, Length=463, Percent_Identity=47.5161987041037, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI106049292, Length=463, Percent_Identity=47.5161987041037, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI38679977, Length=553, Percent_Identity=32.0072332730561, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI38679967, Length=553, Percent_Identity=32.0072332730561, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI38679974, Length=553, Percent_Identity=32.0072332730561, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI38679971, Length=553, Percent_Identity=32.0072332730561, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI38679960, Length=553, Percent_Identity=32.0072332730561, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI134142062, Length=553, Percent_Identity=31.4647377938517, Blast_Score=244, Evalue=2e-64,
Organism=Escherichia coli, GI1789654, Length=444, Percent_Identity=51.3513513513513, Blast_Score=419, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71987519, Length=474, Percent_Identity=49.789029535865, Blast_Score=438, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17567343, Length=444, Percent_Identity=47.5225225225225, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17562816, Length=446, Percent_Identity=44.8430493273543, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI133931226, Length=488, Percent_Identity=30.9426229508197, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI71997163, Length=507, Percent_Identity=30.1775147928994, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71997168, Length=507, Percent_Identity=30.1775147928994, Blast_Score=215, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6319695, Length=445, Percent_Identity=45.8426966292135, Blast_Score=375, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6319685, Length=439, Percent_Identity=44.1913439635535, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6321376, Length=445, Percent_Identity=46.5168539325843, Blast_Score=361, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6323863, Length=456, Percent_Identity=32.8947368421053, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6324343, Length=567, Percent_Identity=31.2169312169312, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24651757, Length=478, Percent_Identity=48.5355648535565, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651759, Length=435, Percent_Identity=48.0459770114943, Blast_Score=407, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281363050, Length=445, Percent_Identity=48.5393258426966, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24652224, Length=445, Percent_Identity=48.5393258426966, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24652222, Length=445, Percent_Identity=48.5393258426966, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24652220, Length=445, Percent_Identity=48.5393258426966, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24652218, Length=445, Percent_Identity=48.5393258426966, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24652212, Length=445, Percent_Identity=48.5393258426966, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24652210, Length=445, Percent_Identity=48.5393258426966, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24652214, Length=445, Percent_Identity=48.5393258426966, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI19921944, Length=445, Percent_Identity=48.5393258426966, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24652216, Length=445, Percent_Identity=48.5393258426966, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI161076409, Length=504, Percent_Identity=32.1428571428571, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI24586458, Length=504, Percent_Identity=32.1428571428571, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI161076407, Length=504, Percent_Identity=32.1428571428571, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24586460, Length=504, Percent_Identity=32.1428571428571, Blast_Score=244, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 55261; Mature: 55130

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKIRKVLVANRGEIAIRVMRTCKELGIATVAVYSEADRSALHVRTADQAIFVGPPPSRE
CCCHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCEEEECCCCCCH
SYLVQQRILDAAKQAGADAIHPGYGFLSENAAFVRACEAEGLTFIGPPASAMDSMGEKTR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCHHHHHHHHHHHH
ARANMIKAGVPVVPGTTEPIPSVEEARAYVEKIGFPVMLKAAGGGGGKGMRKVERMEDFE
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
SSWRAAKSEALNAFGNDAVYIEKYLEKPHHVEIQVFADQYGNTIHLNERECSAQRRHQKV
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCEEEECHHHHHHHHHHHHH
VEETPSPILTPELRAKMGEVAVKAAKAVNYVGAGTVEFLVDVHRNFYFLEMNTRLQVEHP
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCEEEECCC
VTEWVTGLDLVAMQIKAAEGEKLGLTEAPTPRGHSIEVRVYAEDPSRNFMPSPGKITYLR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEE
VPGGPNVRDDSGVFPGYTVPNVYDPMISKLSVWAPTRREAIARTQRALSEYVVKGITTNI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RYLKAILAHPEFIEGDYDTSFLTRQHDALLGAEDPKLSEMALLASVVHTYQKDQKRAKTL
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
PAASGGQGGGNGGVSPWKLALRARR
CCCCCCCCCCCCCCCHHHHHEECCC
>Mature Secondary Structure 
PKIRKVLVANRGEIAIRVMRTCKELGIATVAVYSEADRSALHVRTADQAIFVGPPPSRE
CCHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCEEEECCCCCCH
SYLVQQRILDAAKQAGADAIHPGYGFLSENAAFVRACEAEGLTFIGPPASAMDSMGEKTR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCHHHHHHHHHHHH
ARANMIKAGVPVVPGTTEPIPSVEEARAYVEKIGFPVMLKAAGGGGGKGMRKVERMEDFE
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
SSWRAAKSEALNAFGNDAVYIEKYLEKPHHVEIQVFADQYGNTIHLNERECSAQRRHQKV
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCEEEECHHHHHHHHHHHHH
VEETPSPILTPELRAKMGEVAVKAAKAVNYVGAGTVEFLVDVHRNFYFLEMNTRLQVEHP
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCEEEECCC
VTEWVTGLDLVAMQIKAAEGEKLGLTEAPTPRGHSIEVRVYAEDPSRNFMPSPGKITYLR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEE
VPGGPNVRDDSGVFPGYTVPNVYDPMISKLSVWAPTRREAIARTQRALSEYVVKGITTNI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RYLKAILAHPEFIEGDYDTSFLTRQHDALLGAEDPKLSEMALLASVVHTYQKDQKRAKTL
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
PAASGGQGGGNGGVSPWKLALRARR
CCCCCCCCCCCCCCCHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 9478969 [H]