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The map label for this gene is iam [H]

Identifier: 108762458

GI number: 108762458

Start: 6694023

End: 6696464

Strand: Reverse

Name: iam [H]

Synonym: MXAN_5376

Alternate gene names: 108762458

Gene position: 6696464-6694023 (Counterclockwise)

Preceding gene: 108757077

Following gene: 108759103

Centisome position: 73.27

GC content: 64.78

Gene sequence:

>2442_bases
ATGCGCACTCACAGAATGTCCCGCCTCGTGGGCGCGGCGCTGTCCACCGCCCTGTTCATCACTGGCTGTAGCGAGAGCGA
CTACGTCCGGCTGTACCACAGTGCTGCTCGGGCCCCGAGCTACTCGGTGGACGACATCGAGTCCCTTCGCCAGATCGGCC
CCACCTACGTGGACAAGGGCGTGAACTTCGCCCTGTACTCGGAGAACGCCACCCGGCTGGAGCTGCTGCTCTTCGAGGAC
CCGGAGAGCAACCGCCCGGCACGCGCCTATGAGATGACACGCTATGGCGACGTGTGGAGCGTCTACGTGGAAGGCGTAGG
CGTGGGACAGCACTACGGCTTCCGCGCATGGGGGCCCAACTGGGAGTTCGACCCGCGGTGGTTCCCCGGGTCCATCCACG
GCTTCAAGGCGGACGCGGACGTCTACGGCAACCGCTTCAATCCGAACAAACTGCTCACGGACCCGTACTCCAAGGCCCTG
CACCGCGACCACGACTGGAGCAAGGGCAGCACGGCCAGCGGTCCGGCCCGCACGCAGGTGACTTACGCGGCCTCCGCCAA
GAGCGTGCTCGTCAAGAGCGGCGACTACCAGTGGGGCGAGGTGGAGCAGCAGTGGCGCGCCAACCGCCAGGACGAGAACT
GGCAGGGGCACGGCTGGCAGGACCTCATCGTCTACGAGGTTCACGCCAAGGGCTTCACGGCGGACCCCGCAAGCGGTGTG
CGCTTCCCGGGTACCTACCGCGGCTTCGGTGAGAAGGCGGCCTACCTGGCCGAGCTGGGCATCACCGCGGTGGAGCTGCT
GCCCATCCACGAGAAGCCGCTGGACGGTGGCTACTGGGGCTACCAGACCATCAACTTCTTCGCGCCCGAGCTGTCCTATG
CGGCCTTCAAGGAGCCGCACCAGGTCATCAACGAGTTCAAGTGGATGGTGGAGCAACTCCACAAGCACGGCATCGAGGTG
ATTATCGACGTCGTCTACAACCACACCGGCGAGGGTGGCCTCTGGCGCGAGAAGCTGGAGACGGATGACGTCATGCCCGG
CGAGCCGCTGGAGTCCCTGGACCCCGCGGAGACGGCTGGCCTCTATTCGTTCCGCGGCATCGACAACCAAGCGTACTACG
CGCTCAACCCGGACCGCCGCACGTATTGGAACAACACTGGCGTCGGTAACCAGACGCGCCCCAACCACCGGCCCACGCGC
AAGCTCATCATCGACAGCCTGCGCTTCTACGTGGAAGAGCTCCACGTGGATGGTTTCCGCTTCGACCTGGCGCCCATCCT
GGGTGAGCGGGACGGCGACTACAACCGCTGGGATGACCCGCGCAACACCGTGCTCCAGGACGTCATCGACGACCCGGTGC
TCCAGAAGTACAACACCCGCATCATGGCCGAGCCGTGGAGCGCGGGCGGCTGGTACTGCATGCCGCTGGGCGAGTTCCCC
AACGCCAAGACGCAGCCGGGCAATGGCTGGTACGAGTGGAACGGCCGCTTCCGCGACTGGTGGCGCGCGTTCATGAACCA
GGATGGTTGGAAGCTCAACTCCAACGAGGGTTCGCTGTGCGGCCGGCCTGGCACCGTGGACGGCGGCTTCCTGATGTACG
GCAGCCAGGAGTGGTTCGAGCGCAATGGCCGCCGCCCGTATCACTCCATGAACTTCATCACCGTGCACGACGGCTTCACC
ATGTACGACCTGTTCGCGTACGACGAGAAGCAGAACCGCTGCGGTCCGCTGAACCCGGTGTGCTGTGACACACCGAACAG
TCCCTTCTGCGACAAGGTCAGCGGCGAGGAGCACAACCGTTCCCGCAACTGGGGGCCGATTGGCGACGAGCGCGCGGAGA
GCATGCGGCGGCAGATGATTCGCAATGCCTTCATGTCGATGATGATCAGCCACGGCACGCCGATGATCCTGGGCGGCGAC
GAGTGGATGCGCACGCAGTTGGGGAACAACAACGCCTACTCCACGCTGTCCGACAACCCCTTCAACTGGTACCAGTGGGG
TACGTACCTGGCGCGCGACGAACGTCACCGCATGTTCGAGTTCGTGAAGGCTGCCATCCGCCTGCGCAAGGAGCATGCCT
ATGCCTTCGCTCCGAAGGACTACGGCAAGGGCGCGCCGCTGGCCTGGAAGAGCGCGCAGAACACGGAGGCGGCGTGGGAC
AGCAAGCAGTTGATGATTCACTATCACGACGCGTCCTACGGGCCGGAGTTGCTGGTGCTCATCAACATGGAGCCCCGCGC
GGTGACGTTCACGCTGCCCGAAGGCCGCAAGTGGACGCGGCTCATCGACACGCAGGCGTACTTCGACAGCTCCGCCTATC
TCACGACGGCGGGCCTGGATAGCCGGTCCACGGGTAACTCGTGGCTTGATGCACCGTCACCAGTGAACGGTCCGACGTAC
GGGATGCCGGAAAGAACCATCGTCGTCCTGCGTGCGGAGTAG

Upstream 100 bases:

>100_bases
ACCCGGGCCCTGCCTTGTCAGGCATGGAAGGCGCTTGTTATGGAAACGGAGTTTGATTGCCGGGCCGAGTCATGTCCGGA
CCTCGAGGTCAGCCTACCTG

Downstream 100 bases:

>100_bases
GCAGGCAAGCAGGTTGCGCTATAAGCGGGGCCCGCCCATGCGAGTCCTGCCTGCGCGCCTCGGCGCCATCCTGTTGACGT
TCCTGCCACTCATTGCCCTG

Product: glycosy hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 813; Mature: 813

Protein sequence:

>813_residues
MRTHRMSRLVGAALSTALFITGCSESDYVRLYHSAARAPSYSVDDIESLRQIGPTYVDKGVNFALYSENATRLELLLFED
PESNRPARAYEMTRYGDVWSVYVEGVGVGQHYGFRAWGPNWEFDPRWFPGSIHGFKADADVYGNRFNPNKLLTDPYSKAL
HRDHDWSKGSTASGPARTQVTYAASAKSVLVKSGDYQWGEVEQQWRANRQDENWQGHGWQDLIVYEVHAKGFTADPASGV
RFPGTYRGFGEKAAYLAELGITAVELLPIHEKPLDGGYWGYQTINFFAPELSYAAFKEPHQVINEFKWMVEQLHKHGIEV
IIDVVYNHTGEGGLWREKLETDDVMPGEPLESLDPAETAGLYSFRGIDNQAYYALNPDRRTYWNNTGVGNQTRPNHRPTR
KLIIDSLRFYVEELHVDGFRFDLAPILGERDGDYNRWDDPRNTVLQDVIDDPVLQKYNTRIMAEPWSAGGWYCMPLGEFP
NAKTQPGNGWYEWNGRFRDWWRAFMNQDGWKLNSNEGSLCGRPGTVDGGFLMYGSQEWFERNGRRPYHSMNFITVHDGFT
MYDLFAYDEKQNRCGPLNPVCCDTPNSPFCDKVSGEEHNRSRNWGPIGDERAESMRRQMIRNAFMSMMISHGTPMILGGD
EWMRTQLGNNNAYSTLSDNPFNWYQWGTYLARDERHRMFEFVKAAIRLRKEHAYAFAPKDYGKGAPLAWKSAQNTEAAWD
SKQLMIHYHDASYGPELLVLINMEPRAVTFTLPEGRKWTRLIDTQAYFDSSAYLTTAGLDSRSTGNSWLDAPSPVNGPTY
GMPERTIVVLRAE

Sequences:

>Translated_813_residues
MRTHRMSRLVGAALSTALFITGCSESDYVRLYHSAARAPSYSVDDIESLRQIGPTYVDKGVNFALYSENATRLELLLFED
PESNRPARAYEMTRYGDVWSVYVEGVGVGQHYGFRAWGPNWEFDPRWFPGSIHGFKADADVYGNRFNPNKLLTDPYSKAL
HRDHDWSKGSTASGPARTQVTYAASAKSVLVKSGDYQWGEVEQQWRANRQDENWQGHGWQDLIVYEVHAKGFTADPASGV
RFPGTYRGFGEKAAYLAELGITAVELLPIHEKPLDGGYWGYQTINFFAPELSYAAFKEPHQVINEFKWMVEQLHKHGIEV
IIDVVYNHTGEGGLWREKLETDDVMPGEPLESLDPAETAGLYSFRGIDNQAYYALNPDRRTYWNNTGVGNQTRPNHRPTR
KLIIDSLRFYVEELHVDGFRFDLAPILGERDGDYNRWDDPRNTVLQDVIDDPVLQKYNTRIMAEPWSAGGWYCMPLGEFP
NAKTQPGNGWYEWNGRFRDWWRAFMNQDGWKLNSNEGSLCGRPGTVDGGFLMYGSQEWFERNGRRPYHSMNFITVHDGFT
MYDLFAYDEKQNRCGPLNPVCCDTPNSPFCDKVSGEEHNRSRNWGPIGDERAESMRRQMIRNAFMSMMISHGTPMILGGD
EWMRTQLGNNNAYSTLSDNPFNWYQWGTYLARDERHRMFEFVKAAIRLRKEHAYAFAPKDYGKGAPLAWKSAQNTEAAWD
SKQLMIHYHDASYGPELLVLINMEPRAVTFTLPEGRKWTRLIDTQAYFDSSAYLTTAGLDSRSTGNSWLDAPSPVNGPTY
GMPERTIVVLRAE
>Mature_813_residues
MRTHRMSRLVGAALSTALFITGCSESDYVRLYHSAARAPSYSVDDIESLRQIGPTYVDKGVNFALYSENATRLELLLFED
PESNRPARAYEMTRYGDVWSVYVEGVGVGQHYGFRAWGPNWEFDPRWFPGSIHGFKADADVYGNRFNPNKLLTDPYSKAL
HRDHDWSKGSTASGPARTQVTYAASAKSVLVKSGDYQWGEVEQQWRANRQDENWQGHGWQDLIVYEVHAKGFTADPASGV
RFPGTYRGFGEKAAYLAELGITAVELLPIHEKPLDGGYWGYQTINFFAPELSYAAFKEPHQVINEFKWMVEQLHKHGIEV
IIDVVYNHTGEGGLWREKLETDDVMPGEPLESLDPAETAGLYSFRGIDNQAYYALNPDRRTYWNNTGVGNQTRPNHRPTR
KLIIDSLRFYVEELHVDGFRFDLAPILGERDGDYNRWDDPRNTVLQDVIDDPVLQKYNTRIMAEPWSAGGWYCMPLGEFP
NAKTQPGNGWYEWNGRFRDWWRAFMNQDGWKLNSNEGSLCGRPGTVDGGFLMYGSQEWFERNGRRPYHSMNFITVHDGFT
MYDLFAYDEKQNRCGPLNPVCCDTPNSPFCDKVSGEEHNRSRNWGPIGDERAESMRRQMIRNAFMSMMISHGTPMILGGD
EWMRTQLGNNNAYSTLSDNPFNWYQWGTYLARDERHRMFEFVKAAIRLRKEHAYAFAPKDYGKGAPLAWKSAQNTEAAWD
SKQLMIHYHDASYGPELLVLINMEPRAVTFTLPEGRKWTRLIDTQAYFDSSAYLTTAGLDSRSTGNSWLDAPSPVNGPTY
GMPERTIVVLRAE

Specific function: Has a high rate of hydrolysis for glycogen. Does not cleave pullulan [H]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=656, Percent_Identity=32.0121951219512, Blast_Score=254, Evalue=2e-68,
Organism=Escherichia coli, GI1790687, Length=128, Percent_Identity=30.46875, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.68 [H]

Molecular weight: Translated: 93049; Mature: 93049

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTHRMSRLVGAALSTALFITGCSESDYVRLYHSAARAPSYSVDDIESLRQIGPTYVDKG
CCCHHHHHHHHHHHHHEEEEECCCCCCEEEEEHHHHCCCCCCHHHHHHHHHCCCHHHHCC
VNFALYSENATRLELLLFEDPESNRPARAYEMTRYGDVWSVYVEGVGVGQHYGFRAWGPN
CEEEEECCCCCEEEEEEEECCCCCCCCHHEEHHHCCCEEEEEEEEEECCCCCCEEECCCC
WEFDPRWFPGSIHGFKADADVYGNRFNPNKLLTDPYSKALHRDHDWSKGSTASGPARTQV
CCCCCCCCCCCCCCEECCHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEE
TYAASAKSVLVKSGDYQWGEVEQQWRANRQDENWQGHGWQDLIVYEVHAKGFTADPASGV
EEECCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCC
RFPGTYRGFGEKAAYLAELGITAVELLPIHEKPLDGGYWGYQTINFFAPELSYAAFKEPH
CCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHCCHH
QVINEFKWMVEQLHKHGIEVIIDVVYNHTGEGGLWREKLETDDVMPGEPLESLDPAETAG
HHHHHHHHHHHHHHHCCHHEEEEHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHCC
LYSFRGIDNQAYYALNPDRRTYWNNTGVGNQTRPNHRPTRKLIIDSLRFYVEELHVDGFR
CEEEECCCCCEEEEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
FDLAPILGERDGDYNRWDDPRNTVLQDVIDDPVLQKYNTRIMAEPWSAGGWYCMPLGEFP
EEHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCEEEECCCCCCCEEEEECCCCC
NAKTQPGNGWYEWNGRFRDWWRAFMNQDGWKLNSNEGSLCGRPGTVDGGFLMYGSQEWFE
CCCCCCCCCEEEECCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEEECCHHHHH
RNGRRPYHSMNFITVHDGFTMYDLFAYDEKQNRCGPLNPVCCDTPNSPFCDKVSGEEHNR
CCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCC
SRNWGPIGDERAESMRRQMIRNAFMSMMISHGTPMILGGDEWMRTQLGNNNAYSTLSDNP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHCCCCCCCCCCCCCC
FNWYQWGTYLARDERHRMFEFVKAAIRLRKEHAYAFAPKDYGKGAPLAWKSAQNTEAAWD
CCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCHHCCCCCCEEECCCCCCCCCCC
SKQLMIHYHDASYGPELLVLINMEPRAVTFTLPEGRKWTRLIDTQAYFDSSAYLTTAGLD
CCEEEEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCCEEECHHHHCCCCCEEEECCCC
SRSTGNSWLDAPSPVNGPTYGMPERTIVVLRAE
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MRTHRMSRLVGAALSTALFITGCSESDYVRLYHSAARAPSYSVDDIESLRQIGPTYVDKG
CCCHHHHHHHHHHHHHEEEEECCCCCCEEEEEHHHHCCCCCCHHHHHHHHHCCCHHHHCC
VNFALYSENATRLELLLFEDPESNRPARAYEMTRYGDVWSVYVEGVGVGQHYGFRAWGPN
CEEEEECCCCCEEEEEEEECCCCCCCCHHEEHHHCCCEEEEEEEEEECCCCCCEEECCCC
WEFDPRWFPGSIHGFKADADVYGNRFNPNKLLTDPYSKALHRDHDWSKGSTASGPARTQV
CCCCCCCCCCCCCCEECCHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEE
TYAASAKSVLVKSGDYQWGEVEQQWRANRQDENWQGHGWQDLIVYEVHAKGFTADPASGV
EEECCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCC
RFPGTYRGFGEKAAYLAELGITAVELLPIHEKPLDGGYWGYQTINFFAPELSYAAFKEPH
CCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHCCHH
QVINEFKWMVEQLHKHGIEVIIDVVYNHTGEGGLWREKLETDDVMPGEPLESLDPAETAG
HHHHHHHHHHHHHHHCCHHEEEEHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHCC
LYSFRGIDNQAYYALNPDRRTYWNNTGVGNQTRPNHRPTRKLIIDSLRFYVEELHVDGFR
CEEEECCCCCEEEEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
FDLAPILGERDGDYNRWDDPRNTVLQDVIDDPVLQKYNTRIMAEPWSAGGWYCMPLGEFP
EEHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCEEEECCCCCCCEEEEECCCCC
NAKTQPGNGWYEWNGRFRDWWRAFMNQDGWKLNSNEGSLCGRPGTVDGGFLMYGSQEWFE
CCCCCCCCCEEEECCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEEECCHHHHH
RNGRRPYHSMNFITVHDGFTMYDLFAYDEKQNRCGPLNPVCCDTPNSPFCDKVSGEEHNR
CCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCC
SRNWGPIGDERAESMRRQMIRNAFMSMMISHGTPMILGGDEWMRTQLGNNNAYSTLSDNP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHCCCCCCCCCCCCCC
FNWYQWGTYLARDERHRMFEFVKAAIRLRKEHAYAFAPKDYGKGAPLAWKSAQNTEAAWD
CCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCHHCCCCCCEEECCCCCCCCCCC
SKQLMIHYHDASYGPELLVLINMEPRAVTFTLPEGRKWTRLIDTQAYFDSSAYLTTAGLD
CCEEEEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCCEEECHHHHCCCCCEEEECCCC
SRSTGNSWLDAPSPVNGPTYGMPERTIVVLRAE
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9197405 [H]