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The map label for this gene is glnG [H]

Identifier: 108762137

GI number: 108762137

Start: 6315886

End: 6317475

Strand: Reverse

Name: glnG [H]

Synonym: MXAN_5048

Alternate gene names: 108762137

Gene position: 6317475-6315886 (Counterclockwise)

Preceding gene: 108763322

Following gene: 108763159

Centisome position: 69.12

GC content: 71.89

Gene sequence:

>1590_bases
ATGTCGACCACCGCGGGCCGCGCACAGGCCGGGCAGAGCCCGGTGGCGCTCTCCGTGCCTGCCTTGACGATTGTGTCCCA
TCCCCAGCCGCAGCGCATTGGCGAGCGGCTATTGCTGGAGGTGCTCGCGTCCCAGGGGCGCACAGCGGCCCTGTCTCGCA
ACGCGCCGGACTTCTCCCGGCCCGGGGGGCTCATGGCCCTCCCGCTGGGAGACCCATTCCTCAGCCGCAAGCCGGTGGAG
TTCGAGCCCGGCCCACGAGGCGGCCTCCGGATGTTGGTGCCGGAGGACGGCACCCAGGTGCGCGTGGCGAACGAGCCCGT
CGTGGGCGGCCGGGAGTTCTCTCGCGAGGAGCTGTCCGATGGAGTGCCGCTGGTGCTGGCCGACCGCGTGGTGCTGTTGC
TCCACCTGGCCTCGTCCTTCCGCCAGCCGGGCGTGTTGGACCTGGGCATCGTTGGCCACGGTGAGGGCATCCACCGCGTG
CGCGAGGACATCATCCGGGTGGCCGACCTCGCCGTGCCGGTGCTCATCCGCGGCGAGACGGGCACGGGCAAGGAGCTGGT
GGCGCGCGCCATCCACACCAGTGGTCCCCGCCGCGCCGGTCCCTTCGTCAGCGTCAACCTGGGAGCGCTCGCCAAGGAGC
TGGTTGCCGCGGAGCTCTTTGGCGCGCAGCGCGGGGCCTATACGGGCGCCAGCCGCGACCGGGAAGGCTTCTTCCGCGCG
GCCGAGGGCGGGACGCTCTTCCTCGATGAGGTTGGCGAGGCGCCGCCCGAGGTGCAGGCCGCGCTGCTTCGCGTGCTGGA
GACAGGCGAAATCTATCCGGTGGGCGGCCATACGCCGGTGCCTGTCGACGTCCGGCTGGTCTCCGCCACGGACGCGCATC
TGGAGGCCCGCATCCAGGAGCGCACCTTCAAGGCCCCGCTGCTGCACCGGCTGGCCGGCTTTGAAATCCTGGTGCCTCCG
CTGCGTGAGCGCCGCGAGGACGTGGGCCTGCTCTTCCTCCACTTCTCTCGCCAGGAGCTGGAGGCCATGGGGGAGGGCGG
TCAACTGACGACTTCGGACCCGCGCGCCGAGCCCTGGCTGCCCGCGGCCCTGGCCGTCCAGTTGGTGCGCCACGCGTGGT
CCGGCAACATCCGCCAGTTGCGCAACGTCACCCGTCAGCTCGTCATCGGCAGCCGGGGCACGCCGGGGCTGCGCGCCGAT
GCACGGCTGGAGCAGGTGCTGGACGCGGACGCGCAGCCAGTCCCCGGGCGTCCGTTGACCGTGCGCACGGGTGAGGAGGC
GGGCGATGGCGCGAAGGCACCGCGGCGAAAGCCCGCGGACGTGGGCGAGCAGGAGTTGCTGGAGGCCCTTCGCGCCAGCT
CCTGGGACCTCAAGGCCACCGCGGACTGGCTGGGCATTCCCCGGCCGTCCGTCTACGTCCTCATCGACAAGAGCTCGCTC
CTGCGCACCGCGCGCGATTTGAGCCCCGAGGAAATCACGCGCTGCTTCCACGAGTGCGAGGGCGACCTCGACCGGATGGT
GCAGCGGCTGGAGGTCTCCAAGCGCGCGCTCCAGCGGCGGGTCCGGGAGCTGGGCCTCAGTGGTGGGTAG

Upstream 100 bases:

>100_bases
CTCGGTGGATGAAGCCAGCGCTCGGGCTTCTGGAAGTGTAGGCTCAGCGGGTATCCCAGGACTCACACGAATACCCATGC
AGCAGAAACTCGCCGCAGAC

Downstream 100 bases:

>100_bases
TGTTGGCTGACGCGTCAACCCGGACCTGGTGGGTTCGGTTGACGCGTCCATTTGCCTTTCAGGGGCGGCGCGGGTGTCGA
TTGTCGAGGGGGGCCTCGCG

Product: sigma-54 dependent trancsriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSTTAGRAQAGQSPVALSVPALTIVSHPQPQRIGERLLLEVLASQGRTAALSRNAPDFSRPGGLMALPLGDPFLSRKPVE
FEPGPRGGLRMLVPEDGTQVRVANEPVVGGREFSREELSDGVPLVLADRVVLLLHLASSFRQPGVLDLGIVGHGEGIHRV
REDIIRVADLAVPVLIRGETGTGKELVARAIHTSGPRRAGPFVSVNLGALAKELVAAELFGAQRGAYTGASRDREGFFRA
AEGGTLFLDEVGEAPPEVQAALLRVLETGEIYPVGGHTPVPVDVRLVSATDAHLEARIQERTFKAPLLHRLAGFEILVPP
LRERREDVGLLFLHFSRQELEAMGEGGQLTTSDPRAEPWLPAALAVQLVRHAWSGNIRQLRNVTRQLVIGSRGTPGLRAD
ARLEQVLDADAQPVPGRPLTVRTGEEAGDGAKAPRRKPADVGEQELLEALRASSWDLKATADWLGIPRPSVYVLIDKSSL
LRTARDLSPEEITRCFHECEGDLDRMVQRLEVSKRALQRRVRELGLSGG

Sequences:

>Translated_529_residues
MSTTAGRAQAGQSPVALSVPALTIVSHPQPQRIGERLLLEVLASQGRTAALSRNAPDFSRPGGLMALPLGDPFLSRKPVE
FEPGPRGGLRMLVPEDGTQVRVANEPVVGGREFSREELSDGVPLVLADRVVLLLHLASSFRQPGVLDLGIVGHGEGIHRV
REDIIRVADLAVPVLIRGETGTGKELVARAIHTSGPRRAGPFVSVNLGALAKELVAAELFGAQRGAYTGASRDREGFFRA
AEGGTLFLDEVGEAPPEVQAALLRVLETGEIYPVGGHTPVPVDVRLVSATDAHLEARIQERTFKAPLLHRLAGFEILVPP
LRERREDVGLLFLHFSRQELEAMGEGGQLTTSDPRAEPWLPAALAVQLVRHAWSGNIRQLRNVTRQLVIGSRGTPGLRAD
ARLEQVLDADAQPVPGRPLTVRTGEEAGDGAKAPRRKPADVGEQELLEALRASSWDLKATADWLGIPRPSVYVLIDKSSL
LRTARDLSPEEITRCFHECEGDLDRMVQRLEVSKRALQRRVRELGLSGG
>Mature_528_residues
STTAGRAQAGQSPVALSVPALTIVSHPQPQRIGERLLLEVLASQGRTAALSRNAPDFSRPGGLMALPLGDPFLSRKPVEF
EPGPRGGLRMLVPEDGTQVRVANEPVVGGREFSREELSDGVPLVLADRVVLLLHLASSFRQPGVLDLGIVGHGEGIHRVR
EDIIRVADLAVPVLIRGETGTGKELVARAIHTSGPRRAGPFVSVNLGALAKELVAAELFGAQRGAYTGASRDREGFFRAA
EGGTLFLDEVGEAPPEVQAALLRVLETGEIYPVGGHTPVPVDVRLVSATDAHLEARIQERTFKAPLLHRLAGFEILVPPL
RERREDVGLLFLHFSRQELEAMGEGGQLTTSDPRAEPWLPAALAVQLVRHAWSGNIRQLRNVTRQLVIGSRGTPGLRADA
RLEQVLDADAQPVPGRPLTVRTGEEAGDGAKAPRRKPADVGEQELLEALRASSWDLKATADWLGIPRPSVYVLIDKSSLL
RTARDLSPEEITRCFHECEGDLDRMVQRLEVSKRALQRRVRELGLSGG

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=251, Percent_Identity=44.2231075697211, Blast_Score=208, Evalue=8e-55,
Organism=Escherichia coli, GI1788550, Length=246, Percent_Identity=41.4634146341463, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87082117, Length=253, Percent_Identity=41.1067193675889, Blast_Score=185, Evalue=7e-48,
Organism=Escherichia coli, GI1786524, Length=335, Percent_Identity=38.8059701492537, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1789233, Length=344, Percent_Identity=36.3372093023256, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1788905, Length=270, Percent_Identity=39.6296296296296, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI87081872, Length=346, Percent_Identity=35.8381502890173, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1790437, Length=242, Percent_Identity=41.3223140495868, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789087, Length=245, Percent_Identity=40.4081632653061, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI87082152, Length=246, Percent_Identity=40.650406504065, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1787583, Length=284, Percent_Identity=34.8591549295775, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1789828, Length=254, Percent_Identity=34.251968503937, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI87081858, Length=273, Percent_Identity=32.2344322344322, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 57172; Mature: 57041

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTAGRAQAGQSPVALSVPALTIVSHPQPQRIGERLLLEVLASQGRTAALSRNAPDFSR
CCCCCCCHHCCCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
PGGLMALPLGDPFLSRKPVEFEPGPRGGLRMLVPEDGTQVRVANEPVVGGREFSREELSD
CCCEEEEECCCHHHCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCHHCHHHHHC
GVPLVLADRVVLLLHLASSFRQPGVLDLGIVGHGEGIHRVREDIIRVADLAVPVLIRGET
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHEEEEEECCC
GTGKELVARAIHTSGPRRAGPFVSVNLGALAKELVAAELFGAQRGAYTGASRDREGFFRA
CCCHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
AEGGTLFLDEVGEAPPEVQAALLRVLETGEIYPVGGHTPVPVDVRLVSATDAHLEARIQE
CCCCEEEHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEEECCCHHHHHHHHH
RTFKAPLLHRLAGFEILVPPLRERREDVGLLFLHFSRQELEAMGEGGQLTTSDPRAEPWL
HHHHHHHHHHHCCCEEECCCHHHHHHCCCEEEEEECHHHHHHHCCCCCCCCCCCCCCCCC
PAALAVQLVRHAWSGNIRQLRNVTRQLVIGSRGTPGLRADARLEQVLDADAQPVPGRPLT
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEE
VRTGEEAGDGAKAPRRKPADVGEQELLEALRASSWDLKATADWLGIPRPSVYVLIDKSSL
EECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEECHHHHCCCCCCEEEEECCHHH
LRTARDLSPEEITRCFHECEGDLDRMVQRLEVSKRALQRRVRELGLSGG
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STTAGRAQAGQSPVALSVPALTIVSHPQPQRIGERLLLEVLASQGRTAALSRNAPDFSR
CCCCCCHHCCCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
PGGLMALPLGDPFLSRKPVEFEPGPRGGLRMLVPEDGTQVRVANEPVVGGREFSREELSD
CCCEEEEECCCHHHCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCHHCHHHHHC
GVPLVLADRVVLLLHLASSFRQPGVLDLGIVGHGEGIHRVREDIIRVADLAVPVLIRGET
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHEEEEEECCC
GTGKELVARAIHTSGPRRAGPFVSVNLGALAKELVAAELFGAQRGAYTGASRDREGFFRA
CCCHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
AEGGTLFLDEVGEAPPEVQAALLRVLETGEIYPVGGHTPVPVDVRLVSATDAHLEARIQE
CCCCEEEHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEEECCCHHHHHHHHH
RTFKAPLLHRLAGFEILVPPLRERREDVGLLFLHFSRQELEAMGEGGQLTTSDPRAEPWL
HHHHHHHHHHHCCCEEECCCHHHHHHCCCEEEEEECHHHHHHHCCCCCCCCCCCCCCCCC
PAALAVQLVRHAWSGNIRQLRNVTRQLVIGSRGTPGLRADARLEQVLDADAQPVPGRPLT
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEE
VRTGEEAGDGAKAPRRKPADVGEQELLEALRASSWDLKATADWLGIPRPSVYVLIDKSSL
EECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEECHHHHCCCCCCEEEEECCHHH
LRTARDLSPEEITRCFHECEGDLDRMVQRLEVSKRALQRRVRELGLSGG
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]