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The map label for this gene is kefA [H]

Identifier: 108761998

GI number: 108761998

Start: 3266784

End: 3268361

Strand: Reverse

Name: kefA [H]

Synonym: MXAN_2799

Alternate gene names: 108761998

Gene position: 3268361-3266784 (Counterclockwise)

Preceding gene: 108762151

Following gene: 108759685

Centisome position: 35.76

GC content: 67.93

Gene sequence:

>1578_bases
ATGGCGCGTGTATCCCCGCTCACGCGGCACGCGTCAACGGCCGCCTTGACCCCCATGGAGATGGCGGGAGACATTCCCCC
CGCCTTGCTGCCCTTCCTTCAGAGCAACCTGTCCCTGGCCGTGGGTGCGCTGCTCATCGTCGTGCTGATGGGGGTGCGCG
CCGCCGCTCAAGACGCCGACCTTCGGCAGGATTTGAAGAGCGCCAACCGGCTCCTCATCGCGTTCATCGTGCTCCGGCTG
GCGACGTGGGCCTTGCCGGCGACCGCCCCCGAAGGCCTCAAGAAGGCGCTCCAGGTCAGCTGGATGCTGATGTTCACCTT
CGGTGGCATCCGCGCCAGCGTGGCCCTGGCGCTGAAGGTGATCCGGCTGCGCGCGCCGGCGGTGGGCACACCGAAAATCC
TGCGCAACGTCATCGACTTCACGCTGTACCCACTGGCGGTGCTGCCGCTCCTGCGCACCCAGTTCAACGTGGACCTGGCG
GGCCTGGTGGCCACGTCCGCGGTGCTGTCGGTGGTCATCGGTCTGGCGCTCCAGGAGACGCTGGGCAACCTCTTCGCGGG
CCTGTCCCTCCAACTGGACCGCCCCTTCGAGGTCGGCCACTTCATCCGCATCGGCACACACACCGGACGCGTGGTCTTCA
TTGGCTGGCGCTCCATCCGGCTGGCCAACTTCCGCCGCGAGGTCATCACGCTGCCCAACAGCATGGTGGCCAAGGAGCTG
GTCCTCAACTTCACCCAGGACCCCAACCCCGTCGGCATCGACGTGGAGTTCCGCCTGTCACGGGACGCCGCGCCCAACCA
GGTCAAGCACGCGCTGCTGGAGACGATGCGGGAGACCCCCCTCATCCTCCCGGAGCCGTCTCCCATCGCGCGCACGCTGG
GCTACGAGGATTCCGCCATCCGCTACATGGTGCGGTTCTTCATCGGCGATTACGGCCTGGCGGACGCGGTGAAGGAGGAC
CTCCACTCCCGCCTGTGGTACCGGCTGCGCCGGGCGAACATCGAGATTCCCTACGCCCAGCGGACGGTGCACGTACGACA
GGAGATGGCGCGCACGGAGCTGTCGGAGGACACCATCCGCAGCCTCCTGGGCGCCGTGGACCTCTTCCAGTCCCTGGACA
CGGAGGAGCTGGACCGGCTTCGCCAGGAGTCGCTGGTGCGCCGCTTCGGCGCCGGCGAGCGCATCATCCAGGAGGGCGAC
GAGGGCCGGACCTTCTACGTGCTCGCCTCCGGAGAGGTGAGCGTGCGCACGGGGAAGTCCCAGTCGGAAGTCACCCGCCT
GGGCCGCGGCAGCTACTTCGGAGAGATGTGCCTGCTGACGGGAGAGCGCCGTTCGGCCACGGTGGTGGCGGTGGAGGACT
CATTGCTCCTCGAAGTAGACCGTCCCACCTTCGCCCGCCTCTTCACGGAGTATCCCGGCCTGGCCCGGCAGCTCTCCTCC
ATGCTTGCCCAGCGGCGGACCCAGCTGCGCGCGGTGGCCGAAGCGAGCGGAACGAGCAACGACGCCATTCCCGCCGAGGT
CGGCCGTATACTCGGACGGCTGCGCCAGATATTCGGGCTCAATGCCACACACGAGTAG

Upstream 100 bases:

>100_bases
TGGCCCGGCGAAGAGGTACGCGTGGTGGACCGCGCCGCTGCGCAGGGCCGCCTGGAGGGCGTCCACCGCGCGAGGCTGTC
CCAGTACCGTTGCGAGCGTC

Downstream 100 bases:

>100_bases
GCTGAGCGCAGCGGGGGGATGTCATGACAGACCTAGGTACGCCGGTTCCAAAGCTGGGGCGGGTTCGACCCTCCTCAAAC
TGTGCTGATTTGAACTGCTG

Product: mechanosensitive ion channel/cyclic nucleotide-binding domain-containing protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MARVSPLTRHASTAALTPMEMAGDIPPALLPFLQSNLSLAVGALLIVVLMGVRAAAQDADLRQDLKSANRLLIAFIVLRL
ATWALPATAPEGLKKALQVSWMLMFTFGGIRASVALALKVIRLRAPAVGTPKILRNVIDFTLYPLAVLPLLRTQFNVDLA
GLVATSAVLSVVIGLALQETLGNLFAGLSLQLDRPFEVGHFIRIGTHTGRVVFIGWRSIRLANFRREVITLPNSMVAKEL
VLNFTQDPNPVGIDVEFRLSRDAAPNQVKHALLETMRETPLILPEPSPIARTLGYEDSAIRYMVRFFIGDYGLADAVKED
LHSRLWYRLRRANIEIPYAQRTVHVRQEMARTELSEDTIRSLLGAVDLFQSLDTEELDRLRQESLVRRFGAGERIIQEGD
EGRTFYVLASGEVSVRTGKSQSEVTRLGRGSYFGEMCLLTGERRSATVVAVEDSLLLEVDRPTFARLFTEYPGLARQLSS
MLAQRRTQLRAVAEASGTSNDAIPAEVGRILGRLRQIFGLNATHE

Sequences:

>Translated_525_residues
MARVSPLTRHASTAALTPMEMAGDIPPALLPFLQSNLSLAVGALLIVVLMGVRAAAQDADLRQDLKSANRLLIAFIVLRL
ATWALPATAPEGLKKALQVSWMLMFTFGGIRASVALALKVIRLRAPAVGTPKILRNVIDFTLYPLAVLPLLRTQFNVDLA
GLVATSAVLSVVIGLALQETLGNLFAGLSLQLDRPFEVGHFIRIGTHTGRVVFIGWRSIRLANFRREVITLPNSMVAKEL
VLNFTQDPNPVGIDVEFRLSRDAAPNQVKHALLETMRETPLILPEPSPIARTLGYEDSAIRYMVRFFIGDYGLADAVKED
LHSRLWYRLRRANIEIPYAQRTVHVRQEMARTELSEDTIRSLLGAVDLFQSLDTEELDRLRQESLVRRFGAGERIIQEGD
EGRTFYVLASGEVSVRTGKSQSEVTRLGRGSYFGEMCLLTGERRSATVVAVEDSLLLEVDRPTFARLFTEYPGLARQLSS
MLAQRRTQLRAVAEASGTSNDAIPAEVGRILGRLRQIFGLNATHE
>Mature_524_residues
ARVSPLTRHASTAALTPMEMAGDIPPALLPFLQSNLSLAVGALLIVVLMGVRAAAQDADLRQDLKSANRLLIAFIVLRLA
TWALPATAPEGLKKALQVSWMLMFTFGGIRASVALALKVIRLRAPAVGTPKILRNVIDFTLYPLAVLPLLRTQFNVDLAG
LVATSAVLSVVIGLALQETLGNLFAGLSLQLDRPFEVGHFIRIGTHTGRVVFIGWRSIRLANFRREVITLPNSMVAKELV
LNFTQDPNPVGIDVEFRLSRDAAPNQVKHALLETMRETPLILPEPSPIARTLGYEDSAIRYMVRFFIGDYGLADAVKEDL
HSRLWYRLRRANIEIPYAQRTVHVRQEMARTELSEDTIRSLLGAVDLFQSLDTEELDRLRQESLVRRFGAGERIIQEGDE
GRTFYVLASGEVSVRTGKSQSEVTRLGRGSYFGEMCLLTGERRSATVVAVEDSLLLEVDRPTFARLFTEYPGLARQLSSM
LAQRRTQLRAVAEASGTSNDAIPAEVGRILGRLRQIFGLNATHE

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI5453978, Length=142, Percent_Identity=33.0985915492958, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI4885407, Length=142, Percent_Identity=36.6197183098592, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI4758958, Length=118, Percent_Identity=34.7457627118644, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI156071470, Length=135, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1786670, Length=194, Percent_Identity=24.7422680412371, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789291, Length=194, Percent_Identity=27.319587628866, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787591, Length=203, Percent_Identity=25.1231527093596, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI2367355, Length=186, Percent_Identity=20.9677419354839, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 58026; Mature: 57895

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS00888 CNMP_BINDING_1 ; PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARVSPLTRHASTAALTPMEMAGDIPPALLPFLQSNLSLAVGALLIVVLMGVRAAAQDAD
CCCCCCHHHCCCHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
LRQDLKSANRLLIAFIVLRLATWALPATAPEGLKKALQVSWMLMFTFGGIRASVALALKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRLRAPAVGTPKILRNVIDFTLYPLAVLPLLRTQFNVDLAGLVATSAVLSVVIGLALQET
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LGNLFAGLSLQLDRPFEVGHFIRIGTHTGRVVFIGWRSIRLANFRREVITLPNSMVAKEL
HHHHHHCCEEEECCCCCCCCEEEEECCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHH
VLNFTQDPNPVGIDVEFRLSRDAAPNQVKHALLETMRETPLILPEPSPIARTLGYEDSAI
HHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCEECCCCCHHHHHCCCCHHHH
RYMVRFFIGDYGLADAVKEDLHSRLWYRLRRANIEIPYAQRTVHVRQEMARTELSEDTIR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHH
SLLGAVDLFQSLDTEELDRLRQESLVRRFGAGERIIQEGDEGRTFYVLASGEVSVRTGKS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCEEEEECCCC
QSEVTRLGRGSYFGEMCLLTGERRSATVVAVEDSLLLEVDRPTFARLFTEYPGLARQLSS
HHHHHHHCCCCCCCCCCEEECCCCCEEEEEEECCEEEEECCHHHHHHHHHCCHHHHHHHH
MLAQRRTQLRAVAEASGTSNDAIPAEVGRILGRLRQIFGLNATHE
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARVSPLTRHASTAALTPMEMAGDIPPALLPFLQSNLSLAVGALLIVVLMGVRAAAQDAD
CCCCCHHHCCCHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
LRQDLKSANRLLIAFIVLRLATWALPATAPEGLKKALQVSWMLMFTFGGIRASVALALKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRLRAPAVGTPKILRNVIDFTLYPLAVLPLLRTQFNVDLAGLVATSAVLSVVIGLALQET
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LGNLFAGLSLQLDRPFEVGHFIRIGTHTGRVVFIGWRSIRLANFRREVITLPNSMVAKEL
HHHHHHCCEEEECCCCCCCCEEEEECCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHH
VLNFTQDPNPVGIDVEFRLSRDAAPNQVKHALLETMRETPLILPEPSPIARTLGYEDSAI
HHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCEECCCCCHHHHHCCCCHHHH
RYMVRFFIGDYGLADAVKEDLHSRLWYRLRRANIEIPYAQRTVHVRQEMARTELSEDTIR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHH
SLLGAVDLFQSLDTEELDRLRQESLVRRFGAGERIIQEGDEGRTFYVLASGEVSVRTGKS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCEEEEECCCC
QSEVTRLGRGSYFGEMCLLTGERRSATVVAVEDSLLLEVDRPTFARLFTEYPGLARQLSS
HHHHHHHCCCCCCCCCCEEECCCCCEEEEEEECCEEEEECCHHHHHHHHHCCHHHHHHHH
MLAQRRTQLRAVAEASGTSNDAIPAEVGRILGRLRQIFGLNATHE
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]