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The map label for this gene is gshB [H]

Identifier: 108761672

GI number: 108761672

Start: 18526

End: 19494

Strand: Reverse

Name: gshB [H]

Synonym: MXAN_0017

Alternate gene names: 108761672

Gene position: 19494-18526 (Counterclockwise)

Preceding gene: 108761425

Following gene: 108757004

Centisome position: 0.21

GC content: 69.87

Gene sequence:

>969_bases
ATGGCCCCTCTGACCATTGGCTTCCTGATGGACCCGCTCGAGACGGTGCGGGTGGACCACGACTCCACGTTCGCGTTGAT
GCTCGAAGCGCAGAAGCGCGGCCATCAGGTGCGCTACTTCGAACAGGGCTGGTTGCGCTTCAACGGCACGTGCGCGGAGG
CGCGCATGCGCCACGTCACCGTGCGCCGCGAACTGGGGCGGCACTTCGATATCCTCGACGAGGCCCCTCGCCCGGTGTCC
TCGCTGGACGTGCTCTTCATGCGCAAGGACCCGCCAGTGGACGCGGAGTTCCTGCACGCCACCCAGTTGGTGGAGCTGTG
CGGCACGGGCCGGCCCCCTGTCTTCATCAACGAGCCGGCGGGCCTCCGCGACGCGAACGAGAAGCTCTTCTCCCTGCGCT
ACCCGGACCTGATGCCGGACACGCGCGTCACCAGCGAGCTGCCGGTGCTGCTGGACTTCATTGCGCGCAACGCGGCGGGC
ACCATCCTCAAGCCAGTGGATGGCTTCGCGGGCAAGGGCATCCTCTTCCTGTCCGCCACGGACCGGAACGCGCGCTCCGC
GGTGGACATGCTCACCCGGGGCGGCCGCGAGGCCGTCATGGCGCAGGCGTACATCCCGGAGAGCCGCCAGGGCGACAAGC
GCATCCTCCTGGTGGACGGCGAGCCGGTGGGCGGCGTGCTGCGCGTCCCGTCCGACGACGACCACCGCGGCAACATGGCC
GCCGGCGGCGTGCCCCAGAAGGCCGTGCTCACCCCGCGGGATTTGGAGATTTGCGAGCGCCTGAAGCCGGAGCTGGTGAA
GCGCGGACTGCGGCTGGTGGGCATCGACGTGCTGGGCACCTACCTCACCGAGGTCAACGTGACGAGCCCCACCGGCCTGG
TGGAGGCGAACCAGCTGGACGACGTGTGCGTGGAGGCCAAGGTGCTGGACGTGGCGGAGCGGCTCGTCGCCGAGCGCGCC
GCGCGCTGA

Upstream 100 bases:

>100_bases
GCGGAAATCCCCGAGCGCTGAAACACAGAACCGGGATTGAGGGGCACGTCCCGGGGCACTTGGTTATCTTCGCGCCCCTC
TCCCTCTGATTCCGGACTGT

Downstream 100 bases:

>100_bases
GCCCCGCCGGAGGACGCCGGGCGGCCCGGCGCCCGGTGTCTTCCCTGAGCTGAGGGCTGACTCATGTTTCAGCGCACCAC
ACGACGCGGTGCGTGATTTG

Product: glutathione synthase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MAPLTIGFLMDPLETVRVDHDSTFALMLEAQKRGHQVRYFEQGWLRFNGTCAEARMRHVTVRRELGRHFDILDEAPRPVS
SLDVLFMRKDPPVDAEFLHATQLVELCGTGRPPVFINEPAGLRDANEKLFSLRYPDLMPDTRVTSELPVLLDFIARNAAG
TILKPVDGFAGKGILFLSATDRNARSAVDMLTRGGREAVMAQAYIPESRQGDKRILLVDGEPVGGVLRVPSDDDHRGNMA
AGGVPQKAVLTPRDLEICERLKPELVKRGLRLVGIDVLGTYLTEVNVTSPTGLVEANQLDDVCVEAKVLDVAERLVAERA
AR

Sequences:

>Translated_322_residues
MAPLTIGFLMDPLETVRVDHDSTFALMLEAQKRGHQVRYFEQGWLRFNGTCAEARMRHVTVRRELGRHFDILDEAPRPVS
SLDVLFMRKDPPVDAEFLHATQLVELCGTGRPPVFINEPAGLRDANEKLFSLRYPDLMPDTRVTSELPVLLDFIARNAAG
TILKPVDGFAGKGILFLSATDRNARSAVDMLTRGGREAVMAQAYIPESRQGDKRILLVDGEPVGGVLRVPSDDDHRGNMA
AGGVPQKAVLTPRDLEICERLKPELVKRGLRLVGIDVLGTYLTEVNVTSPTGLVEANQLDDVCVEAKVLDVAERLVAERA
AR
>Mature_321_residues
APLTIGFLMDPLETVRVDHDSTFALMLEAQKRGHQVRYFEQGWLRFNGTCAEARMRHVTVRRELGRHFDILDEAPRPVSS
LDVLFMRKDPPVDAEFLHATQLVELCGTGRPPVFINEPAGLRDANEKLFSLRYPDLMPDTRVTSELPVLLDFIARNAAGT
ILKPVDGFAGKGILFLSATDRNARSAVDMLTRGGREAVMAQAYIPESRQGDKRILLVDGEPVGGVLRVPSDDDHRGNMAA
GGVPQKAVLTPRDLEICERLKPELVKRGLRLVGIDVLGTYLTEVNVTSPTGLVEANQLDDVCVEAKVLDVAERLVAERAA
R

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=311, Percent_Identity=39.2282958199357, Blast_Score=224, Evalue=7e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35555; Mature: 35424

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPLTIGFLMDPLETVRVDHDSTFALMLEAQKRGHQVRYFEQGWLRFNGTCAEARMRHVT
CCCEEEHHHHCCHHHEEECCCCEEEEEEEHHHCCCEEEEEECCCEEECCCHHHHHHHHHH
VRRELGRHFDILDEAPRPVSSLDVLFMRKDPPVDAEFLHATQLVELCGTGRPPVFINEPA
HHHHHCCCCCHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEECCCC
GLRDANEKLFSLRYPDLMPDTRVTSELPVLLDFIARNAAGTILKPVDGFAGKGILFLSAT
CCCCCCHHHEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCEEEEEEC
DRNARSAVDMLTRGGREAVMAQAYIPESRQGDKRILLVDGEPVGGVLRVPSDDDHRGNMA
CCCHHHHHHHHHCCCHHHEEHHHCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCC
AGGVPQKAVLTPRDLEICERLKPELVKRGLRLVGIDVLGTYLTEVNVTSPTGLVEANQLD
CCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCEEEHHHHHHHHHEEECCCCCCCEECCCHH
DVCVEAKVLDVAERLVAERAAR
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
APLTIGFLMDPLETVRVDHDSTFALMLEAQKRGHQVRYFEQGWLRFNGTCAEARMRHVT
CCEEEHHHHCCHHHEEECCCCEEEEEEEHHHCCCEEEEEECCCEEECCCHHHHHHHHHH
VRRELGRHFDILDEAPRPVSSLDVLFMRKDPPVDAEFLHATQLVELCGTGRPPVFINEPA
HHHHHCCCCCHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEECCCC
GLRDANEKLFSLRYPDLMPDTRVTSELPVLLDFIARNAAGTILKPVDGFAGKGILFLSAT
CCCCCCHHHEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCEEEEEEC
DRNARSAVDMLTRGGREAVMAQAYIPESRQGDKRILLVDGEPVGGVLRVPSDDDHRGNMA
CCCHHHHHHHHHCCCHHHEEHHHCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCC
AGGVPQKAVLTPRDLEICERLKPELVKRGLRLVGIDVLGTYLTEVNVTSPTGLVEANQLD
CCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCEEEHHHHHHHHHEEECCCCCCCEECCCHH
DVCVEAKVLDVAERLVAERAAR
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]