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The map label for this gene is otsAB [H]

Identifier: 108761665

GI number: 108761665

Start: 1389169

End: 1391337

Strand: Direct

Name: otsAB [H]

Synonym: MXAN_1192

Alternate gene names: 108761665

Gene position: 1389169-1391337 (Clockwise)

Preceding gene: 108759229

Following gene: 108764094

Centisome position: 15.2

GC content: 69.29

Gene sequence:

>2169_bases
ATGTCCCGACTCCTACTCGTCTCGAATCGACTTCCTGTCACCGTCAAGGTGGAGAAGGACACTGTCTCCGTGGTCCGCAG
CGCGGGAGGCCTGGCCACCGGCCTTCGCCGTCCGCACGAACGCTCGGGGGGGCTGTGGATTGGCTGGCCCGGGGACGTCT
CCCGCTTGTCGGAAAGCCAACGCGCCCGCGTGGACGCCCAGCTCGCGGAGCTGCGCTGCGTGCCGCTGACGCTGTCCGCC
AGCGAGGTGAGCCGCTACTACGAAGGCTATTCCAACCGCGTCCTGTGGCCGCTGTGCCACTACATGCTGGAGCGCATTCC
GCGCCAGGACCGCGATTGGGAGGTGTACCGCAAGGTCAACGAGCGCTTCGCGGACCTGGTGGCGAAGCACTACGAGCCCG
GGGACACCATCTGGGTCCACGACTATCAGCTCATGCTGGTGCCTGGCATGTTGCGCCAGCGGTTGCCCGGCGCGCGCATC
GGCTACTTCCACCACATCCCGTTTCCGTCGTCGGAAATCTTCAGCACGCTGCCGCGTCGGCGTGAGCTGCTGAAGGGGCT
GCTGGGCGCGGACCTCATCGGCTTCCACACGGTGAGCTACGTGCGGCACTTCTCCGGAGCGCTGCTGCGGCACCTCGGGC
TGGACACGGACATCGACCGCATCATCTGGGAGGGGCGCGACGTGCGCGTGGGCGCCTTCCCCATGGGCATCGACGCGCAG
GCCTTCGAATCGATTGCGAGCGAGCCCGGCATGCAGGAGGAGGTGGCGAACTTGCGCCGGTCCTCCGAAGGACAGCGCAT
CCTGTTGGGCATCGACCGGCTGGACTACACCAAGGGCATTCCGCGGCGCCTCCTGGCGGTGCAGCGCGTGCTGGAGCGCA
CGCCCGCGTGGCGTGGCCGCCTGCGTTTCATCCAGGTGACGGTACCCAGCCGGACCCAGGTGGAGGCGTACGCCAACTAC
CGCGAGCTGGTGAACGAGCTGGTGGGGCGCATCAATGGGCTCTACGGGACGGTGCACAACGTGCCCGTGCACTACCTCTA
CCGCTCATTCAACGAACGGCAGCTCGTGGGGTTGTACCGGGGCGCGGATGTGATGCTCGTCACGCCGGTGCGGGACGGGA
TGAACCTGGTGGCGAAGGAGTTCTGCGCGGCGCGTCCGGACGACGACGGCGTGCTGGTGCTCAGCGAGTTCGCGGGCGCC
GCAGCGGAGCTGGGGGGCGCGCTCATCGTCAACCCCTACGACGTGGACGCCATGGCGGACGCCATCGAGAAAGCGCTGGG
GATGGCGGAGGAGGAGCGCCGCACGCGCATGCACACGCTGCGCAAGCAGGTGAAGTCGCGGGACGTGCATTGGTGGACGT
CCTCCTTCCTCGACCGGCTCCAGTCGCTGCCCGCGGTGGACGTGCGCGCCGAGGCGAGCGCGCCCGAGGCGCTGGCGCAG
ATGAAGCAGGTGGAGCGGCTGCAACTGCTCCTGGACTACGACGGGACGCTGGTGGGCTACGCGCCCCGGCCGGAGCTGGC
CGCGCCGGATGCGGCGCTGAAGGAGTTGCTGGCGAAGCTGGTGGCCCGGCCCGGCATCTCGGTGAGCATCGTCAGCGGCC
GGCCCAAGGAGACGCTGGAGGAATGGTTGGGCGACCTGGCCATGGGCCTGTATGCCGAGCACGGCCTGTGGTCCCGCCCG
GCGCCGGGGGAGGCGTGGAGGATGCTGGAGGGCGTGACGTTCGACTGGAAGGAGCGCGTGCGCCCCGAACTGGAGACCTT
CTCCGCGCGCGTGCCGGGCTCCTTCGTGGAGGAGAAGACAGCGTCGCTGGCGTGGCACTACCGCCTGGTGGACGCGGAGT
TCGGCGCCATCCAGTCCCGCGAGCTGCGGCTGTACCTGGCGGAGAAGTTCGCAGGGCAGTCCATGGACATCCTCCCCGGG
GACAAGGTGGTGGAGATTCGTCCGCGGGGCGTGCACAAGGGCCGCGTGGTGGGCGAGGCCACGAAGGACGCGGCGCCCGG
CGCCCTCGTGGTGGCCATCGGCGATGACCGCACGGACGAGGACCTCTTCGCATCCATTCCGCCCGGCGGGCTCACCATCC
ACGCGGGCAACAAGCCCACGCGCGCGGCGTTCCGGGTGAATGGCCCGGACGAGGTCCGCGCGCTGCTGGCGGGTCTGCTG
GAGCCGTAA

Upstream 100 bases:

>100_bases
GGACACCGATGGCCACCCGACACGAGGGACCAACATCTGGCATGAAGCCCGTTTCGACGATGGCCATTCCTGCACGGAGT
GACTAATTCAGGAGGGGCCT

Downstream 100 bases:

>100_bases
CCACCTCGCGCCCGCCGGAGGTTCGGCGGGCGCGGGCCTGCTCGCGTTCGCTACGGCGTCGAGGACGCGGTGCGGTTGGC
CGCGTTCTGGATGAACACCC

Product: putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB

Products: H+; alpha,alpha'-Trehalose 6-phosphate; UDP [C]

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 722; Mature: 721

Protein sequence:

>722_residues
MSRLLLVSNRLPVTVKVEKDTVSVVRSAGGLATGLRRPHERSGGLWIGWPGDVSRLSESQRARVDAQLAELRCVPLTLSA
SEVSRYYEGYSNRVLWPLCHYMLERIPRQDRDWEVYRKVNERFADLVAKHYEPGDTIWVHDYQLMLVPGMLRQRLPGARI
GYFHHIPFPSSEIFSTLPRRRELLKGLLGADLIGFHTVSYVRHFSGALLRHLGLDTDIDRIIWEGRDVRVGAFPMGIDAQ
AFESIASEPGMQEEVANLRRSSEGQRILLGIDRLDYTKGIPRRLLAVQRVLERTPAWRGRLRFIQVTVPSRTQVEAYANY
RELVNELVGRINGLYGTVHNVPVHYLYRSFNERQLVGLYRGADVMLVTPVRDGMNLVAKEFCAARPDDDGVLVLSEFAGA
AAELGGALIVNPYDVDAMADAIEKALGMAEEERRTRMHTLRKQVKSRDVHWWTSSFLDRLQSLPAVDVRAEASAPEALAQ
MKQVERLQLLLDYDGTLVGYAPRPELAAPDAALKELLAKLVARPGISVSIVSGRPKETLEEWLGDLAMGLYAEHGLWSRP
APGEAWRMLEGVTFDWKERVRPELETFSARVPGSFVEEKTASLAWHYRLVDAEFGAIQSRELRLYLAEKFAGQSMDILPG
DKVVEIRPRGVHKGRVVGEATKDAAPGALVVAIGDDRTDEDLFASIPPGGLTIHAGNKPTRAAFRVNGPDEVRALLAGLL
EP

Sequences:

>Translated_722_residues
MSRLLLVSNRLPVTVKVEKDTVSVVRSAGGLATGLRRPHERSGGLWIGWPGDVSRLSESQRARVDAQLAELRCVPLTLSA
SEVSRYYEGYSNRVLWPLCHYMLERIPRQDRDWEVYRKVNERFADLVAKHYEPGDTIWVHDYQLMLVPGMLRQRLPGARI
GYFHHIPFPSSEIFSTLPRRRELLKGLLGADLIGFHTVSYVRHFSGALLRHLGLDTDIDRIIWEGRDVRVGAFPMGIDAQ
AFESIASEPGMQEEVANLRRSSEGQRILLGIDRLDYTKGIPRRLLAVQRVLERTPAWRGRLRFIQVTVPSRTQVEAYANY
RELVNELVGRINGLYGTVHNVPVHYLYRSFNERQLVGLYRGADVMLVTPVRDGMNLVAKEFCAARPDDDGVLVLSEFAGA
AAELGGALIVNPYDVDAMADAIEKALGMAEEERRTRMHTLRKQVKSRDVHWWTSSFLDRLQSLPAVDVRAEASAPEALAQ
MKQVERLQLLLDYDGTLVGYAPRPELAAPDAALKELLAKLVARPGISVSIVSGRPKETLEEWLGDLAMGLYAEHGLWSRP
APGEAWRMLEGVTFDWKERVRPELETFSARVPGSFVEEKTASLAWHYRLVDAEFGAIQSRELRLYLAEKFAGQSMDILPG
DKVVEIRPRGVHKGRVVGEATKDAAPGALVVAIGDDRTDEDLFASIPPGGLTIHAGNKPTRAAFRVNGPDEVRALLAGLL
EP
>Mature_721_residues
SRLLLVSNRLPVTVKVEKDTVSVVRSAGGLATGLRRPHERSGGLWIGWPGDVSRLSESQRARVDAQLAELRCVPLTLSAS
EVSRYYEGYSNRVLWPLCHYMLERIPRQDRDWEVYRKVNERFADLVAKHYEPGDTIWVHDYQLMLVPGMLRQRLPGARIG
YFHHIPFPSSEIFSTLPRRRELLKGLLGADLIGFHTVSYVRHFSGALLRHLGLDTDIDRIIWEGRDVRVGAFPMGIDAQA
FESIASEPGMQEEVANLRRSSEGQRILLGIDRLDYTKGIPRRLLAVQRVLERTPAWRGRLRFIQVTVPSRTQVEAYANYR
ELVNELVGRINGLYGTVHNVPVHYLYRSFNERQLVGLYRGADVMLVTPVRDGMNLVAKEFCAARPDDDGVLVLSEFAGAA
AELGGALIVNPYDVDAMADAIEKALGMAEEERRTRMHTLRKQVKSRDVHWWTSSFLDRLQSLPAVDVRAEASAPEALAQM
KQVERLQLLLDYDGTLVGYAPRPELAAPDAALKELLAKLVARPGISVSIVSGRPKETLEEWLGDLAMGLYAEHGLWSRPA
PGEAWRMLEGVTFDWKERVRPELETFSARVPGSFVEEKTASLAWHYRLVDAEFGAIQSRELRLYLAEKFAGQSMDILPGD
KVVEIRPRGVHKGRVVGEATKDAAPGALVVAIGDDRTDEDLFASIPPGGLTIHAGNKPTRAAFRVNGPDEVRALLAGLLE
P

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=466, Percent_Identity=38.1974248927039, Blast_Score=304, Evalue=1e-83,
Organism=Escherichia coli, GI1788207, Length=229, Percent_Identity=30.1310043668122, Blast_Score=73, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71987012, Length=421, Percent_Identity=31.5914489311164, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI115533560, Length=420, Percent_Identity=29.0476190476191, Blast_Score=160, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI115533558, Length=420, Percent_Identity=29.0476190476191, Blast_Score=160, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6319602, Length=465, Percent_Identity=41.7204301075269, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6320279, Length=688, Percent_Identity=32.4127906976744, Blast_Score=355, Evalue=1e-98,
Organism=Saccharomyces cerevisiae, GI6323537, Length=584, Percent_Identity=30.6506849315068, Blast_Score=271, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6323917, Length=548, Percent_Identity=30.2919708029197, Blast_Score=263, Evalue=5e-71,
Organism=Drosophila melanogaster, GI19920676, Length=762, Percent_Identity=33.4645669291339, Blast_Score=364, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15 [C]

Molecular weight: Translated: 80782; Mature: 80650

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLLLVSNRLPVTVKVEKDTVSVVRSAGGLATGLRRPHERSGGLWIGWPGDVSRLSESQ
CCCEEEEECCCCEEEEECHHHHHHHHHCCCHHHHHCCCCCCCCCEEEECCCCHHHHHHHH
RARVDAQLAELRCVPLTLSASEVSRYYEGYSNRVLWPLCHYMLERIPRQDRDWEVYRKVN
HHHHHHHHHHEEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
ERFADLVAKHYEPGDTIWVHDYQLMLVPGMLRQRLPGARIGYFHHIPFPSSEIFSTLPRR
HHHHHHHHHHCCCCCEEEEECEEEEECCHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHH
RELLKGLLGADLIGFHTVSYVRHFSGALLRHLGLDTDIDRIIWEGRDVRVGAFPMGIDAQ
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCCCCCCHH
AFESIASEPGMQEEVANLRRSSEGQRILLGIDRLDYTKGIPRRLLAVQRVLERTPAWRGR
HHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECHHHHHCCCHHHHHHHHHHHHHCCCCCCC
LRFIQVTVPSRTQVEAYANYRELVNELVGRINGLYGTVHNVPVHYLYRSFNERQLVGLYR
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEC
GADVMLVTPVRDGMNLVAKEFCAARPDDDGVLVLSEFAGAAAELGGALIVNPYDVDAMAD
CCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEHHHCCHHHHCCCEEEECCCCHHHHHH
AIEKALGMAEEERRTRMHTLRKQVKSRDVHWWTSSFLDRLQSLPAVDVRAEASAPEALAQ
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEECCCCCHHHHHH
MKQVERLQLLLDYDGTLVGYAPRPELAAPDAALKELLAKLVARPGISVSIVSGRPKETLE
HHHHHHHHHHEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHH
EWLGDLAMGLYAEHGLWSRPAPGEAWRMLEGVTFDWKERVRPELETFSARVPGSFVEEKT
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHCCCHHHHHHCCCCCHHHHHHH
ASLAWHYRLVDAEFGAIQSRELRLYLAEKFAGQSMDILPGDKVVEIRPRGVHKGRVVGEA
HHHEEEEEEEECCCCCCCCCHHEEEEHHHHCCCCCCCCCCCCEEEECCCCCCCCCEECCC
TKDAAPGALVVAIGDDRTDEDLFASIPPGGLTIHAGNKPTRAAFRVNGPDEVRALLAGLL
CCCCCCCEEEEEECCCCCCCCCEEECCCCCEEEECCCCCCCEEEEECCHHHHHHHHHHHC
EP
CC
>Mature Secondary Structure 
SRLLLVSNRLPVTVKVEKDTVSVVRSAGGLATGLRRPHERSGGLWIGWPGDVSRLSESQ
CCEEEEECCCCEEEEECHHHHHHHHHCCCHHHHHCCCCCCCCCEEEECCCCHHHHHHHH
RARVDAQLAELRCVPLTLSASEVSRYYEGYSNRVLWPLCHYMLERIPRQDRDWEVYRKVN
HHHHHHHHHHEEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
ERFADLVAKHYEPGDTIWVHDYQLMLVPGMLRQRLPGARIGYFHHIPFPSSEIFSTLPRR
HHHHHHHHHHCCCCCEEEEECEEEEECCHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHH
RELLKGLLGADLIGFHTVSYVRHFSGALLRHLGLDTDIDRIIWEGRDVRVGAFPMGIDAQ
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCCCCCCHH
AFESIASEPGMQEEVANLRRSSEGQRILLGIDRLDYTKGIPRRLLAVQRVLERTPAWRGR
HHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECHHHHHCCCHHHHHHHHHHHHHCCCCCCC
LRFIQVTVPSRTQVEAYANYRELVNELVGRINGLYGTVHNVPVHYLYRSFNERQLVGLYR
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEC
GADVMLVTPVRDGMNLVAKEFCAARPDDDGVLVLSEFAGAAAELGGALIVNPYDVDAMAD
CCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEHHHCCHHHHCCCEEEECCCCHHHHHH
AIEKALGMAEEERRTRMHTLRKQVKSRDVHWWTSSFLDRLQSLPAVDVRAEASAPEALAQ
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEECCCCCHHHHHH
MKQVERLQLLLDYDGTLVGYAPRPELAAPDAALKELLAKLVARPGISVSIVSGRPKETLE
HHHHHHHHHHEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHH
EWLGDLAMGLYAEHGLWSRPAPGEAWRMLEGVTFDWKERVRPELETFSARVPGSFVEEKT
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHCCCHHHHHHCCCCCHHHHHHH
ASLAWHYRLVDAEFGAIQSRELRLYLAEKFAGQSMDILPGDKVVEIRPRGVHKGRVVGEA
HHHEEEEEEEECCCCCCCCCHHEEEEHHHHCCCCCCCCCCCCEEEECCCCCCCCCEECCC
TKDAAPGALVVAIGDDRTDEDLFASIPPGGLTIHAGNKPTRAAFRVNGPDEVRALLAGLL
CCCCCCCEEEEEECCCCCCCCCEEECCCCCEEEECCCCCCCEEEEECCHHHHHHHHHHHC
EP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Glucose 6-phosphate; UDPglucose [C]

Specific reaction: D-Glucose 6-phosphate + UDPglucose --> H+ + alpha,alpha'-Trehalose 6-phosphate + UDP [C]

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA