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The map label for this gene is miaA

Identifier: 108761579

GI number: 108761579

Start: 4447119

End: 4448045

Strand: Direct

Name: miaA

Synonym: MXAN_3731

Alternate gene names: 108761579

Gene position: 4447119-4448045 (Clockwise)

Preceding gene: 108760208

Following gene: 108762641

Centisome position: 48.66

GC content: 69.69

Gene sequence:

>927_bases
ATGGGTGAGGGTGGAGCGCAGCGGCCACTGTTGACGGTGATTGCCGGGCCGACGGCGTCGGGGAAGACGGCGCTGGCGAT
TGCGCTGGCTCAGCGTGCTGGTGGCGAGATTGTCAGCGCGGACTCGCAGCAGGTGTATCGGCACTTCGATATCGGCACGG
CGAAGCCCTCCTCGGAGGAGCTGGCTGCGGTGCCTCATCACCTGGTGTCCGCCGTGGACCCGATGGAGGCGTTCTCCGCT
GTCGAGTACCAGCGCCGTGCGGACGCCGTCATCGCGGAGATTGCCGCACGCGGCCGGCCTGTCTTCGTCGTGGGTGGCAC
CGGGCTGTATTTGCGCGTGTTGCTTCACGGTGTGCTCGAGGCGCCCGGGGCCTTGCCGGAGTTGAGGGCGGAGCTGGAGG
CGCTGGCGGCCGGTGAGGGGCGCGAGGCGGTGCATCGGAGGCTGGCGGAGGTGGACCCGGAGACGGCGGCGAAGCTTCCT
CCGCAGGACCTGGTCCGCGTCATCCGGGCGCTGGAGATTCATGCGCAGACAGGCGTGCCCGCCTCGGCGTTCCGGAAGGC
CCATGCCTTCGCGCCGGACCGCTATCCCTTCCAACTGTACGTTCTGTCGCCACCGCGCGACGTGCTGTACGGGCTCATCA
ACACGCGCACGCGGATGTTGTTCGAGCGTGGGCTCGTCGAGGAGACCCGCGAGCTGCTGGCGCGGGGGTACGCCGATTCG
GCGCCCATGCGCAGCGTGGGCTATGTGCAGGCGCGCGCCGTCGTTGAGGGGCGGATGACGCGCGAAGAGGCCATTCACGA
CACCGCGCAGGAGACGCGCCGCTACGCCAAGCGGCAGTTGACGTGGTTCCGGAAGGAGCCCGGGGCTGTCTTCCTGGCTC
CCCCGTACGAGGCGGCGCTTCCGCTCGGCGGCTGTTGCTCGCCCTGA

Upstream 100 bases:

>100_bases
CTTGGCGCGGCGGGACGGTTGCTGTCAGAGGTTTCGCCCGTGCGGTTGGTGTTCGCGCGTGATGCGTCCGCGGCGGCGGT
GATTCAGGGAGCGTTGGCGA

Downstream 100 bases:

>100_bases
CGGCCCAAAGGTCGGGGCGGGCGGGGGACGGAGCGGTGTCAGCGAGCGTTGTCGGCTCCAACGCGGGAGCCCATGTTCGG
CGATGGATGTGCCGGGTTCC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MGEGGAQRPLLTVIAGPTASGKTALAIALAQRAGGEIVSADSQQVYRHFDIGTAKPSSEELAAVPHHLVSAVDPMEAFSA
VEYQRRADAVIAEIAARGRPVFVVGGTGLYLRVLLHGVLEAPGALPELRAELEALAAGEGREAVHRRLAEVDPETAAKLP
PQDLVRVIRALEIHAQTGVPASAFRKAHAFAPDRYPFQLYVLSPPRDVLYGLINTRTRMLFERGLVEETRELLARGYADS
APMRSVGYVQARAVVEGRMTREEAIHDTAQETRRYAKRQLTWFRKEPGAVFLAPPYEAALPLGGCCSP

Sequences:

>Translated_308_residues
MGEGGAQRPLLTVIAGPTASGKTALAIALAQRAGGEIVSADSQQVYRHFDIGTAKPSSEELAAVPHHLVSAVDPMEAFSA
VEYQRRADAVIAEIAARGRPVFVVGGTGLYLRVLLHGVLEAPGALPELRAELEALAAGEGREAVHRRLAEVDPETAAKLP
PQDLVRVIRALEIHAQTGVPASAFRKAHAFAPDRYPFQLYVLSPPRDVLYGLINTRTRMLFERGLVEETRELLARGYADS
APMRSVGYVQARAVVEGRMTREEAIHDTAQETRRYAKRQLTWFRKEPGAVFLAPPYEAALPLGGCCSP
>Mature_307_residues
GEGGAQRPLLTVIAGPTASGKTALAIALAQRAGGEIVSADSQQVYRHFDIGTAKPSSEELAAVPHHLVSAVDPMEAFSAV
EYQRRADAVIAEIAARGRPVFVVGGTGLYLRVLLHGVLEAPGALPELRAELEALAAGEGREAVHRRLAEVDPETAAKLPP
QDLVRVIRALEIHAQTGVPASAFRKAHAFAPDRYPFQLYVLSPPRDVLYGLINTRTRMLFERGLVEETRELLARGYADSA
PMRSVGYVQARAVVEGRMTREEAIHDTAQETRRYAKRQLTWFRKEPGAVFLAPPYEAALPLGGCCSP

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=331, Percent_Identity=33.8368580060423, Blast_Score=149, Evalue=2e-36,
Organism=Escherichia coli, GI1790613, Length=292, Percent_Identity=40.7534246575342, Blast_Score=199, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI25144712, Length=185, Percent_Identity=30.2702702702703, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=29.8611111111111, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24649830, Length=333, Percent_Identity=29.4294294294294, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_MYXXD (Q1D607)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_631916.1
- ProteinModelPortal:   Q1D607
- SMR:   Q1D607
- STRING:   Q1D607
- GeneID:   4103801
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_3731
- TIGR:   MXAN_3731
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q1D607
- ProtClustDB:   PRK00091
- BioCyc:   MXAN246197:MXAN_3731-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33330; Mature: 33198

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEGGAQRPLLTVIAGPTASGKTALAIALAQRAGGEIVSADSQQVYRHFDIGTAKPSSEE
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEECCCHHHHHHHHCCCCCCCCHHH
LAAVPHHLVSAVDPMEAFSAVEYQRRADAVIAEIAARGRPVFVVGGTGLYLRVLLHGVLE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHH
APGALPELRAELEALAAGEGREAVHRRLAEVDPETAAKLPPQDLVRVIRALEIHAQTGVP
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCCCC
ASAFRKAHAFAPDRYPFQLYVLSPPRDVLYGLINTRTRMLFERGLVEETRELLARGYADS
HHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
APMRSVGYVQARAVVEGRMTREEAIHDTAQETRRYAKRQLTWFRKEPGAVFLAPPYEAAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHC
PLGGCCSP
CCCCCCCC
>Mature Secondary Structure 
GEGGAQRPLLTVIAGPTASGKTALAIALAQRAGGEIVSADSQQVYRHFDIGTAKPSSEE
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEECCCHHHHHHHHCCCCCCCCHHH
LAAVPHHLVSAVDPMEAFSAVEYQRRADAVIAEIAARGRPVFVVGGTGLYLRVLLHGVLE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHH
APGALPELRAELEALAAGEGREAVHRRLAEVDPETAAKLPPQDLVRVIRALEIHAQTGVP
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCCCC
ASAFRKAHAFAPDRYPFQLYVLSPPRDVLYGLINTRTRMLFERGLVEETRELLARGYADS
HHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
APMRSVGYVQARAVVEGRMTREEAIHDTAQETRRYAKRQLTWFRKEPGAVFLAPPYEAAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHC
PLGGCCSP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA