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The map label for this gene is pilT [H]

Identifier: 108761365

GI number: 108761365

Start: 476067

End: 477161

Strand: Direct

Name: pilT [H]

Synonym: MXAN_0415

Alternate gene names: 108761365

Gene position: 476067-477161 (Clockwise)

Preceding gene: 108762067

Following gene: 108763493

Centisome position: 5.21

GC content: 64.2

Gene sequence:

>1095_bases
TTGCTGACGAGGGTCACTCCCCTGGATCTGGCAACGCTCAACAAGCTGCTCGCGGTCGGTGTGCAGAACGGTGCATCGGA
CATCCACTTCCGGCCGGGGGATCCGCCCATCTACCGGGTCAATGGCGCGCTCCGGCCCTTGAAGATGGAAAAGCTTCACG
CGGACCACACGCGACAGGTGGCAGTCCATGTCGTGTCGGACCCCGAGGTGAAGAAGCAGATTGACACGCTTCAGGAGTGC
GACGCGTCCTACAGCCTCCCAGGTGTGGCGCGCTTCCGGGTGAATATCTACCGGCAGCGGGGCTCGCTGGCGTGCATCCT
GCGCATCATCCCGGATGAAATCCCAACCATTGACGGACTGGGATTGCCGCAGGTGTTGAAGAAGATTGCCGGCAACGAGC
GCGGGTTGGTGCTGGTGACGGGCGCGACGGGCTCGGGGAAGAGCTCCACGCTGGCGGCGATGATTGACCACATCAATCGC
ACGGAGAACCTGCACGTCCTCACCATCGAGGACCCCATCGAGTTCATCTACAAGAACATCAAGTCCTCCATCTCCCAGCG
GGAGATTGGCCCGGACACGCAGAGCTTCGCGATTGCGCTGCGCGCGGCGCTGCGCCAGGACCCGGACGTCATCCTGGTAG
GCGAGATGCGCGACACGGAGACCATCGACATCGCGCTCAAGGCGTCGGAGACGGGCCACCTGGTGCTCTCCACGGTGCAC
ACGACGGATGCGTCACGCACCATCAACCGCCTGGTGTCGGTGTTCCCCGCCGAAGAGCAGACAATGGTGCGCATGCGCCT
GGCGGACTGCCTCAAGGCCACGGTGTCGCAGCGCCTGCTGCCCCGGGCCAGCGGCAAGGGCCGCACCGTGGCGTTGGAAA
TCATGGTGCAGACGAAGACGGTGGAGCAGTACATCCGCGACGACCGCGCCAACGAGCTGAAGGACGTCATCGAGAAGGGC
CGAGACATGTTCGGCATGCAGTCCTTCGACCAGCACCTGACGCAGCTCTATCGGGAGGGGATCATCACCCTGGAGACGGC
GCAGAGCGCGGCCTCCAACCCGGCCGACTTCCAGCGCGCGCTCGAGTTCGAGTGA

Upstream 100 bases:

>100_bases
GCCCTCGCCTGAGATTGCGGAACTGACCCTGGTGTCTGCCCCGGGGTAATTGCGCACCATGTGCAACTGTTGAGTATCCG
CCTAGAGTCCGGGGCCTTCC

Downstream 100 bases:

>100_bases
GCGGCGCGGCTGTTGCCCTGGGTGAGCCCGCGAAGGCCGCGGGTACGCGGCAATCCCCCTGTGGAAACAGTAGGGTCATT
CCAGACCCGATACGCTTGAA

Product: twitching mobility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MLTRVTPLDLATLNKLLAVGVQNGASDIHFRPGDPPIYRVNGALRPLKMEKLHADHTRQVAVHVVSDPEVKKQIDTLQEC
DASYSLPGVARFRVNIYRQRGSLACILRIIPDEIPTIDGLGLPQVLKKIAGNERGLVLVTGATGSGKSSTLAAMIDHINR
TENLHVLTIEDPIEFIYKNIKSSISQREIGPDTQSFAIALRAALRQDPDVILVGEMRDTETIDIALKASETGHLVLSTVH
TTDASRTINRLVSVFPAEEQTMVRMRLADCLKATVSQRLLPRASGKGRTVALEIMVQTKTVEQYIRDDRANELKDVIEKG
RDMFGMQSFDQHLTQLYREGIITLETAQSAASNPADFQRALEFE

Sequences:

>Translated_364_residues
MLTRVTPLDLATLNKLLAVGVQNGASDIHFRPGDPPIYRVNGALRPLKMEKLHADHTRQVAVHVVSDPEVKKQIDTLQEC
DASYSLPGVARFRVNIYRQRGSLACILRIIPDEIPTIDGLGLPQVLKKIAGNERGLVLVTGATGSGKSSTLAAMIDHINR
TENLHVLTIEDPIEFIYKNIKSSISQREIGPDTQSFAIALRAALRQDPDVILVGEMRDTETIDIALKASETGHLVLSTVH
TTDASRTINRLVSVFPAEEQTMVRMRLADCLKATVSQRLLPRASGKGRTVALEIMVQTKTVEQYIRDDRANELKDVIEKG
RDMFGMQSFDQHLTQLYREGIITLETAQSAASNPADFQRALEFE
>Mature_364_residues
MLTRVTPLDLATLNKLLAVGVQNGASDIHFRPGDPPIYRVNGALRPLKMEKLHADHTRQVAVHVVSDPEVKKQIDTLQEC
DASYSLPGVARFRVNIYRQRGSLACILRIIPDEIPTIDGLGLPQVLKKIAGNERGLVLVTGATGSGKSSTLAAMIDHINR
TENLHVLTIEDPIEFIYKNIKSSISQREIGPDTQSFAIALRAALRQDPDVILVGEMRDTETIDIALKASETGHLVLSTVH
TTDASRTINRLVSVFPAEEQTMVRMRLADCLKATVSQRLLPRASGKGRTVALEIMVQTKTVEQYIRDDRANELKDVIEKG
RDMFGMQSFDQHLTQLYREGIITLETAQSAASNPADFQRALEFE

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=331, Percent_Identity=39.5770392749245, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1786296, Length=284, Percent_Identity=34.1549295774648, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789723, Length=296, Percent_Identity=32.7702702702703, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40294; Mature: 40294

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRVTPLDLATLNKLLAVGVQNGASDIHFRPGDPPIYRVNGALRPLKMEKLHADHTRQV
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEECCCCCCHHHHHHCCCCCEEE
AVHVVSDPEVKKQIDTLQECDASYSLPGVARFRVNIYRQRGSLACILRIIPDEIPTIDGL
EEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHEEEEEECCCCEEEEEEECCCCCCCCCCC
GLPQVLKKIAGNERGLVLVTGATGSGKSSTLAAMIDHINRTENLHVLTIEDPIEFIYKNI
CHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHH
KSSISQREIGPDTQSFAIALRAALRQDPDVILVGEMRDTETIDIALKASETGHLVLSTVH
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEECCCCCEEEEEEE
TTDASRTINRLVSVFPAEEQTMVRMRLADCLKATVSQRLLPRASGKGRTVALEIMVQTKT
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEHHH
VEQYIRDDRANELKDVIEKGRDMFGMQSFDQHLTQLYREGIITLETAQSAASNPADFQRA
HHHHHHCCCHHHHHHHHHHCHHHCCHHHHHHHHHHHHHCCCEEEEECHHCCCCCHHHHHH
LEFE
HCCC
>Mature Secondary Structure
MLTRVTPLDLATLNKLLAVGVQNGASDIHFRPGDPPIYRVNGALRPLKMEKLHADHTRQV
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEECCCCCCHHHHHHCCCCCEEE
AVHVVSDPEVKKQIDTLQECDASYSLPGVARFRVNIYRQRGSLACILRIIPDEIPTIDGL
EEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHEEEEEECCCCEEEEEEECCCCCCCCCCC
GLPQVLKKIAGNERGLVLVTGATGSGKSSTLAAMIDHINRTENLHVLTIEDPIEFIYKNI
CHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHH
KSSISQREIGPDTQSFAIALRAALRQDPDVILVGEMRDTETIDIALKASETGHLVLSTVH
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEECCCCCEEEEEEE
TTDASRTINRLVSVFPAEEQTMVRMRLADCLKATVSQRLLPRASGKGRTVALEIMVQTKT
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEHHH
VEQYIRDDRANELKDVIEKGRDMFGMQSFDQHLTQLYREGIITLETAQSAASNPADFQRA
HHHHHHCCCHHHHHHHHHHCHHHCCHHHHHHHHHHHHHCCCEEEEECHHCCCCCHHHHHH
LEFE
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]