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The map label for this gene is metH [C]

Identifier: 108761329

GI number: 108761329

Start: 281523

End: 282185

Strand: Reverse

Name: metH [C]

Synonym: MXAN_0232

Alternate gene names: 108761329

Gene position: 282185-281523 (Counterclockwise)

Preceding gene: 108758903

Following gene: 108761928

Centisome position: 3.09

GC content: 68.33

Gene sequence:

>663_bases
GTGCTCACCTCCCATGACTCCGCCCTGACGGGCCTGCTGAACGCGTATCTCGCAGCGCAGCTCGCCGGGAACCGGCGTGA
GGCGTTGCGGCTGCTCGTGGACGAGGGACTGCTCCGGGACATCCCCCTCCAGACGCTGCACCTGGAGGTCATCCAGCCCG
CCCAGTATGAAATCGGCCGGCTGTGGCAGGAGAACGTCATCTCCGTGGCGCAGGAGCACCTGGCGACCGCCGTCTCCCAG
CTCGCGCTCGCGCACCTGTACCGGCACCTGCCCAGGGATCCGTTCAACGGCAAGGTCGTCCTCGTCTCCTGCGTCCAGGG
CGAGCTGCATGAAGTGGGCGCGCGCATGTCCAGCGACTTCCTGGAGATGGCCGGCTTCGACGTGCGCTTCCTGGGCGCCA
ACGTTCCACCGGAGCACCTGGCCCGCATGACGAACGAGCTTCGTCCGGACCTGGTCGCCCTTTCCGTGACGATGACGTAC
CACCTGCCCGCGCTCCGTGAAGCCGTGGCGCACGTGCGCAAGACACTCCCGGATGTCCCCATCGCCGTGGGCGGCCTGGC
CTTCAACTGGGCACCCGGACTGGAGGAGGAGCTGGGCGTGAACTTCTTCGGCAAGGACGCGCGGCAGTTCGTCGCGTCCG
CCTGCCGGGCCCTGGGAGTCTGA

Upstream 100 bases:

>100_bases
CCGGGCACTGTCAGGTTGGCGATGAAATCGGGGCCAGTTCACTGTAACTGAGAAACGCAACCCCGGTACGTTGCCCCCTC
GCCCTTCCCCCACGGCGCCC

Downstream 100 bases:

>100_bases
AAGCACCATGGAGAGCGTGAGCAAGAGCGTGGAAGCGCGGGCGGACCGGCTGGCCACCGCCTCTCAGGAGGCGCTCTATC
AGGACCCCTTCTGGGCCGCC

Product: B12-binding domain-containing protein

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: Regulatory Protein MerR; Homocysteine S-Methyltransferase; MerR Family Transcriptional Regulator; Methionine Synthase; 5-Methyltetrahydrofolate S-Homocysteine Methyltransferase; Transcriptional Regulator MerR Family; Transcriptional Regulator; MerR Family Regulatory Protein; Methionine Synthase I Methyltransferase Subunit; Transcriptional Regulator CarH; 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; Helix-Turn-Helix Domain Protein; Methyltransferase Cognate Corrinoid Protein; 5-Methyltetrahydrofolate-Homocysteine Methyltransferase; Transcriptional Regulator XRE Family; Transcriptional Regulator Marr Family; Response Regulator Receiver Protein

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MLTSHDSALTGLLNAYLAAQLAGNRREALRLLVDEGLLRDIPLQTLHLEVIQPAQYEIGRLWQENVISVAQEHLATAVSQ
LALAHLYRHLPRDPFNGKVVLVSCVQGELHEVGARMSSDFLEMAGFDVRFLGANVPPEHLARMTNELRPDLVALSVTMTY
HLPALREAVAHVRKTLPDVPIAVGGLAFNWAPGLEEELGVNFFGKDARQFVASACRALGV

Sequences:

>Translated_220_residues
MLTSHDSALTGLLNAYLAAQLAGNRREALRLLVDEGLLRDIPLQTLHLEVIQPAQYEIGRLWQENVISVAQEHLATAVSQ
LALAHLYRHLPRDPFNGKVVLVSCVQGELHEVGARMSSDFLEMAGFDVRFLGANVPPEHLARMTNELRPDLVALSVTMTY
HLPALREAVAHVRKTLPDVPIAVGGLAFNWAPGLEEELGVNFFGKDARQFVASACRALGV
>Mature_220_residues
MLTSHDSALTGLLNAYLAAQLAGNRREALRLLVDEGLLRDIPLQTLHLEVIQPAQYEIGRLWQENVISVAQEHLATAVSQ
LALAHLYRHLPRDPFNGKVVLVSCVQGELHEVGARMSSDFLEMAGFDVRFLGANVPPEHLARMTNELRPDLVALSVTMTY
HLPALREAVAHVRKTLPDVPIAVGGLAFNWAPGLEEELGVNFFGKDARQFVASACRALGV

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 24127; Mature: 24127

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTSHDSALTGLLNAYLAAQLAGNRREALRLLVDEGLLRDIPLQTLHLEVIQPAQYEIGR
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHCCHHHHHHH
LWQENVISVAQEHLATAVSQLALAHLYRHLPRDPFNGKVVLVSCVQGELHEVGARMSSDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
LEMAGFDVRFLGANVPPEHLARMTNELRPDLVALSVTMTYHLPALREAVAHVRKTLPDVP
HHHCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IAVGGLAFNWAPGLEEELGVNFFGKDARQFVASACRALGV
EEECCEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTSHDSALTGLLNAYLAAQLAGNRREALRLLVDEGLLRDIPLQTLHLEVIQPAQYEIGR
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHCCHHHHHHH
LWQENVISVAQEHLATAVSQLALAHLYRHLPRDPFNGKVVLVSCVQGELHEVGARMSSDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
LEMAGFDVRFLGANVPPEHLARMTNELRPDLVALSVTMTYHLPALREAVAHVRKTLPDVP
HHHCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IAVGGLAFNWAPGLEEELGVNFFGKDARQFVASACRALGV
EEECCEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} 0.035 {S-methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA