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The map label for this gene is leuS [H]

Identifier: 108761325

GI number: 108761325

Start: 5943324

End: 5945819

Strand: Reverse

Name: leuS [H]

Synonym: MXAN_4745

Alternate gene names: 108761325

Gene position: 5945819-5943324 (Counterclockwise)

Preceding gene: 108763466

Following gene: 108757452

Centisome position: 65.05

GC content: 67.51

Gene sequence:

>2496_bases
ATGAACGAGCGTTACGAGCCGCAGGCGATTGAAGGTAAGTGGCAGACCCGTTGGGACGACGCGGGCATCTTCCGGGCAGG
CAGCCGCCCCGGTGCACCCAAGAAGTACGTGCTCGAGATGCTGCCGTACCCCAGCGGGAAGATGCACATGGGGCATGTCC
GCAACTACCTGCTCGGGGACGTGTACGCGCGTTACTTCCGGATGAAGGGCTTCGACGTGCTGCATCCCATGGGCTGGGAT
GCGCTCGGCCTGCCGGCCGAGAACGCGGCCATCAAGGACGGCGTGCACCCGGCGGTGCGCACGGCCGAGAACATCGCGTC
CTTCAAGAAGGAAATCAAAAGCCTGGGCTACTGCTACGACTGGGATCGCGAGGTCAACACCAGCGATCCCGAGTACTACC
GCTGGAATCAGTGGTTCTTCATCCAGATGCTGGAGCGTGGGCTCGTCTATCGCCGCTTCAGCAAGGTGAACTGGTGTACG
GGCTGCCACACCGTCATCGCCAACGAGCAGGTGAAGGACGGCGTCTGCGAGCGCTGCGACTCCCCCGTGGTGGACAAGGA
GATGCCCGAGTGGGCGTTCCGCATCACCCGGTTCTCGCAGGAGTTGCTGGACGCGCTGGACACGCTCAAGGAGTGGCCCC
ACCGCATCACCGCCGCGCAGCGGAACTGGATTGGCCGTTCGGACGGTGCCGAGGCCGACTTCCGCGTGCAGGGGCACGAC
ACCGCGCTGCGCGTGTTCACCACCCGCATCGACACCATCTTCGGCTGCACCTACGTGGTGCTGGCGCCGGACCACGAGTT
GGTGGCCCAGGTGACGACGCCGGAGCGCCGCGCGGAGGTGGAAGCCTTCGCCAAGCGCATGGCCGCGCAGTCCAAGACGG
AGCGCCTGGGCGAGGGCACGGAGAAGGAGGGCGTCTTTACTGGCGCCTACGCGGAGAATCCCTTCACCGGCCAGGCGGTC
CCCATCTGGATCGCCAACTTCGTGCTGAGCGACTACGGCACCGGCGCGGTGATGAGCGTGCCCGCGCATGACGAGCGCGA
CTTCGCCTTCGCGCGCAAGTACAGCCTGCCTGTGAAGGTCGTCATCCAGCCCGCGTCCGGTGACAAGCTCCCGCCGGGTG
ACAAGCTGGAGGCGGCCTATACGGAGTACGGCGTGCTGGAGGACTCGGGCGCCTACACGGGCCAGACATCCGAGCAGGCG
CGTCAGGCCATGGCCGCGTTGCTGGAGAAGGACGGCAAGGGCAAGGCGACGGTGACGTACCGCCAGAAGGACTGGGGCTT
CAGCCGCCAGCGCTACTGGGGCACGCCCATCCCCATCGTCTACTGCGAGAAGTGCGACCCGGACCGCAATGGCATCCCGG
TGCCGGTGGCGCAGCTGCCCGTGCGCCTGCCGGAGATCGACACCCAGGCGGTGCTCACCGGCAAGGGTGAGCCGCCGCTG
GCGAAGGTGGCCTCGTGGGTGAACACCTCGTGTCCGAAGTGCGGCGGGCCTGCCCGGCGCGAGGCGGAGACGATGGACAC
CTTCGTTGACTCCTGCTGGTACTTCGCGCGCTACCTGTCGCCGCACTACGACGCCGCGCCGTTCGACCCGAAGGAGGCCC
AGCGCTTCCTGCCCGTGGACATCTACGTGGGGGGGCCCGAGCACGCGGTGATGCACCTGCTCTATTTCCGCTTCTGGACG
CGGGTGATGAAGCTGTTGGGGCTGAGCCCCGTGGATGAGCCCGTCAAGCGCCTCATCACCCAGGGCATCGTCAACGGGCC
GGATGGCCGGAAGATGTCCAAGCGCTGGGGCAACGTGGTGGCGCCTGCCTCCATCGTCCAGAAGTACGGCGCGGACACGG
CGCGGGCCTACGTGTTGTTCGCGGGCCCGCCAGAGCGGGACTTCGACTGGTCCGATGAGCAGGTGGAGGGCGTGTACCGC
TTCCTCAAGCGCGTCTGGGCGCTGGCCGCCACGCACCAGGCGGCGGCCGCGGGTGCCACCCATGACGGGCCCTACGAGGG
CAAGGCGCTGGAGATCCTCCGCGCCGCCCACAAGACGCTGAAGCGGGTGGGGGAGGCCATCGAGCGCCTGTCCTTCAACA
CGGCCATCGCCGGCATCATGGAAGGCCTCAACGCGCTCTACGCACTGGGGACACCTGAAACGGCCGCGGAGAAGGCCGCC
ATGGCGGAGGCCGTGCGGTTGCTGGCGGTTGTGCTCACGCCGTTCACGCCGCACATCGCGGACGAAATCGCGGAGGCCTA
TGGCAGCAAGGACTTCACCGTCGCGCAGTCCTGGCCGGAGTTCGATCCGGCGCTGGTGGTGGACGACGTCATCCCCTACG
CCGTGCAGGTGAACGGCAAGCTGCGCGCGGAGATTCGCGTGGCGGCGGACGCGGCGGAGGCGGATGTTCGCGCCGCGGCA
GAAGCGGACGAAAAGGTCCAGGTGGCCCTGGCGGGGAAGACGCTGCGCAAGTTCGTCTTCGTCCCCAAGCGCCTCGTGAA
CTTCGTCGCGACCTGA

Upstream 100 bases:

>100_bases
CAGCCGATCCTGGGGCCGCCGTCCGTCCGGGACGGATTTCTCCGGGCGAGACTGTTGCCTGGGGCACGTCCCTCCTCTAA
GAGACTCGATGGGTATGGTG

Downstream 100 bases:

>100_bases
GCGGGGATCCGGGTGCCGGCCGAGGTCCTCGTCATGTGTGGTGCATGTGGGCGGCCCCAACCCGCGGGCCGCCCGCGGTG
CATTGCCTGCGAGGCCGTGC

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 831; Mature: 831

Protein sequence:

>831_residues
MNERYEPQAIEGKWQTRWDDAGIFRAGSRPGAPKKYVLEMLPYPSGKMHMGHVRNYLLGDVYARYFRMKGFDVLHPMGWD
ALGLPAENAAIKDGVHPAVRTAENIASFKKEIKSLGYCYDWDREVNTSDPEYYRWNQWFFIQMLERGLVYRRFSKVNWCT
GCHTVIANEQVKDGVCERCDSPVVDKEMPEWAFRITRFSQELLDALDTLKEWPHRITAAQRNWIGRSDGAEADFRVQGHD
TALRVFTTRIDTIFGCTYVVLAPDHELVAQVTTPERRAEVEAFAKRMAAQSKTERLGEGTEKEGVFTGAYAENPFTGQAV
PIWIANFVLSDYGTGAVMSVPAHDERDFAFARKYSLPVKVVIQPASGDKLPPGDKLEAAYTEYGVLEDSGAYTGQTSEQA
RQAMAALLEKDGKGKATVTYRQKDWGFSRQRYWGTPIPIVYCEKCDPDRNGIPVPVAQLPVRLPEIDTQAVLTGKGEPPL
AKVASWVNTSCPKCGGPARREAETMDTFVDSCWYFARYLSPHYDAAPFDPKEAQRFLPVDIYVGGPEHAVMHLLYFRFWT
RVMKLLGLSPVDEPVKRLITQGIVNGPDGRKMSKRWGNVVAPASIVQKYGADTARAYVLFAGPPERDFDWSDEQVEGVYR
FLKRVWALAATHQAAAAGATHDGPYEGKALEILRAAHKTLKRVGEAIERLSFNTAIAGIMEGLNALYALGTPETAAEKAA
MAEAVRLLAVVLTPFTPHIADEIAEAYGSKDFTVAQSWPEFDPALVVDDVIPYAVQVNGKLRAEIRVAADAAEADVRAAA
EADEKVQVALAGKTLRKFVFVPKRLVNFVAT

Sequences:

>Translated_831_residues
MNERYEPQAIEGKWQTRWDDAGIFRAGSRPGAPKKYVLEMLPYPSGKMHMGHVRNYLLGDVYARYFRMKGFDVLHPMGWD
ALGLPAENAAIKDGVHPAVRTAENIASFKKEIKSLGYCYDWDREVNTSDPEYYRWNQWFFIQMLERGLVYRRFSKVNWCT
GCHTVIANEQVKDGVCERCDSPVVDKEMPEWAFRITRFSQELLDALDTLKEWPHRITAAQRNWIGRSDGAEADFRVQGHD
TALRVFTTRIDTIFGCTYVVLAPDHELVAQVTTPERRAEVEAFAKRMAAQSKTERLGEGTEKEGVFTGAYAENPFTGQAV
PIWIANFVLSDYGTGAVMSVPAHDERDFAFARKYSLPVKVVIQPASGDKLPPGDKLEAAYTEYGVLEDSGAYTGQTSEQA
RQAMAALLEKDGKGKATVTYRQKDWGFSRQRYWGTPIPIVYCEKCDPDRNGIPVPVAQLPVRLPEIDTQAVLTGKGEPPL
AKVASWVNTSCPKCGGPARREAETMDTFVDSCWYFARYLSPHYDAAPFDPKEAQRFLPVDIYVGGPEHAVMHLLYFRFWT
RVMKLLGLSPVDEPVKRLITQGIVNGPDGRKMSKRWGNVVAPASIVQKYGADTARAYVLFAGPPERDFDWSDEQVEGVYR
FLKRVWALAATHQAAAAGATHDGPYEGKALEILRAAHKTLKRVGEAIERLSFNTAIAGIMEGLNALYALGTPETAAEKAA
MAEAVRLLAVVLTPFTPHIADEIAEAYGSKDFTVAQSWPEFDPALVVDDVIPYAVQVNGKLRAEIRVAADAAEADVRAAA
EADEKVQVALAGKTLRKFVFVPKRLVNFVAT
>Mature_831_residues
MNERYEPQAIEGKWQTRWDDAGIFRAGSRPGAPKKYVLEMLPYPSGKMHMGHVRNYLLGDVYARYFRMKGFDVLHPMGWD
ALGLPAENAAIKDGVHPAVRTAENIASFKKEIKSLGYCYDWDREVNTSDPEYYRWNQWFFIQMLERGLVYRRFSKVNWCT
GCHTVIANEQVKDGVCERCDSPVVDKEMPEWAFRITRFSQELLDALDTLKEWPHRITAAQRNWIGRSDGAEADFRVQGHD
TALRVFTTRIDTIFGCTYVVLAPDHELVAQVTTPERRAEVEAFAKRMAAQSKTERLGEGTEKEGVFTGAYAENPFTGQAV
PIWIANFVLSDYGTGAVMSVPAHDERDFAFARKYSLPVKVVIQPASGDKLPPGDKLEAAYTEYGVLEDSGAYTGQTSEQA
RQAMAALLEKDGKGKATVTYRQKDWGFSRQRYWGTPIPIVYCEKCDPDRNGIPVPVAQLPVRLPEIDTQAVLTGKGEPPL
AKVASWVNTSCPKCGGPARREAETMDTFVDSCWYFARYLSPHYDAAPFDPKEAQRFLPVDIYVGGPEHAVMHLLYFRFWT
RVMKLLGLSPVDEPVKRLITQGIVNGPDGRKMSKRWGNVVAPASIVQKYGADTARAYVLFAGPPERDFDWSDEQVEGVYR
FLKRVWALAATHQAAAAGATHDGPYEGKALEILRAAHKTLKRVGEAIERLSFNTAIAGIMEGLNALYALGTPETAAEKAA
MAEAVRLLAVVLTPFTPHIADEIAEAYGSKDFTVAQSWPEFDPALVVDDVIPYAVQVNGKLRAEIRVAADAAEADVRAAA
EADEKVQVALAGKTLRKFVFVPKRLVNFVAT

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=882, Percent_Identity=33.9002267573696, Blast_Score=475, Evalue=1e-134,
Organism=Escherichia coli, GI1786861, Length=877, Percent_Identity=44.6978335233751, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=190, Percent_Identity=24.2105263157895, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71997517, Length=800, Percent_Identity=28.5, Blast_Score=305, Evalue=9e-83,
Organism=Caenorhabditis elegans, GI71997510, Length=800, Percent_Identity=28.5, Blast_Score=304, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI212645227, Length=324, Percent_Identity=29.0123456790123, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323414, Length=862, Percent_Identity=35.0348027842227, Blast_Score=504, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6321531, Length=871, Percent_Identity=21.8140068886338, Blast_Score=121, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6319395, Length=692, Percent_Identity=22.8323699421965, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325217, Length=140, Percent_Identity=30.7142857142857, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21355409, Length=858, Percent_Identity=32.0512820512821, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI17864482, Length=676, Percent_Identity=22.9289940828402, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24653289, Length=676, Percent_Identity=22.9289940828402, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 92678; Mature: 92678

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNERYEPQAIEGKWQTRWDDAGIFRAGSRPGAPKKYVLEMLPYPSGKMHMGHVRNYLLGD
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHCCCCCCCEEHHHHHHHHHHH
VYARYFRMKGFDVLHPMGWDALGLPAENAAIKDGVHPAVRTAENIASFKKEIKSLGYCYD
HHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEC
WDREVNTSDPEYYRWNQWFFIQMLERGLVYRRFSKVNWCTGCHTVIANEQVKDGVCERCD
CCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHCCCHHHHHHHHHCC
SPVVDKEMPEWAFRITRFSQELLDALDTLKEWPHRITAAQRNWIGRSDGAEADFRVQGHD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCC
TALRVFTTRIDTIFGCTYVVLAPDHELVAQVTTPERRAEVEAFAKRMAAQSKTERLGEGT
CEEHHHHHHHHHHHCCEEEEECCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCC
EKEGVFTGAYAENPFTGQAVPIWIANFVLSDYGTGAVMSVPAHDERDFAFARKYSLPVKV
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHCCCCEEE
VIQPASGDKLPPGDKLEAAYTEYGVLEDSGAYTGQTSEQARQAMAALLEKDGKGKATVTY
EEEECCCCCCCCCCCCHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
RQKDWGFSRQRYWGTPIPIVYCEKCDPDRNGIPVPVAQLPVRLPEIDTQAVLTGKGEPPL
ECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCEEEECCCCCCH
AKVASWVNTSCPKCGGPARREAETMDTFVDSCWYFARYLSPHYDAAPFDPKEAQRFLPVD
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEEEE
IYVGGPEHAVMHLLYFRFWTRVMKLLGLSPVDEPVKRLITQGIVNGPDGRKMSKRWGNVV
EEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCC
APASIVQKYGADTARAYVLFAGPPERDFDWSDEQVEGVYRFLKRVWALAATHQAAAAGAT
CHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HDGPYEGKALEILRAAHKTLKRVGEAIERLSFNTAIAGIMEGLNALYALGTPETAAEKAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHH
MAEAVRLLAVVLTPFTPHIADEIAEAYGSKDFTVAQSWPEFDPALVVDDVIPYAVQVNGK
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEHHCCCCEEEEECCE
LRAEIRVAADAAEADVRAAAEADEKVQVALAGKTLRKFVFVPKRLVNFVAT
EEEEEEEECCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNERYEPQAIEGKWQTRWDDAGIFRAGSRPGAPKKYVLEMLPYPSGKMHMGHVRNYLLGD
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHCCCCCCCEEHHHHHHHHHHH
VYARYFRMKGFDVLHPMGWDALGLPAENAAIKDGVHPAVRTAENIASFKKEIKSLGYCYD
HHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEC
WDREVNTSDPEYYRWNQWFFIQMLERGLVYRRFSKVNWCTGCHTVIANEQVKDGVCERCD
CCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHCCCHHHHHHHHHCC
SPVVDKEMPEWAFRITRFSQELLDALDTLKEWPHRITAAQRNWIGRSDGAEADFRVQGHD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCC
TALRVFTTRIDTIFGCTYVVLAPDHELVAQVTTPERRAEVEAFAKRMAAQSKTERLGEGT
CEEHHHHHHHHHHHCCEEEEECCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCC
EKEGVFTGAYAENPFTGQAVPIWIANFVLSDYGTGAVMSVPAHDERDFAFARKYSLPVKV
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHCCCCEEE
VIQPASGDKLPPGDKLEAAYTEYGVLEDSGAYTGQTSEQARQAMAALLEKDGKGKATVTY
EEEECCCCCCCCCCCCHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
RQKDWGFSRQRYWGTPIPIVYCEKCDPDRNGIPVPVAQLPVRLPEIDTQAVLTGKGEPPL
ECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCEEEECCCCCCH
AKVASWVNTSCPKCGGPARREAETMDTFVDSCWYFARYLSPHYDAAPFDPKEAQRFLPVD
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEEEE
IYVGGPEHAVMHLLYFRFWTRVMKLLGLSPVDEPVKRLITQGIVNGPDGRKMSKRWGNVV
EEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCC
APASIVQKYGADTARAYVLFAGPPERDFDWSDEQVEGVYRFLKRVWALAATHQAAAAGAT
CHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HDGPYEGKALEILRAAHKTLKRVGEAIERLSFNTAIAGIMEGLNALYALGTPETAAEKAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHH
MAEAVRLLAVVLTPFTPHIADEIAEAYGSKDFTVAQSWPEFDPALVVDDVIPYAVQVNGK
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEHHCCCCEEEEECCE
LRAEIRVAADAAEADVRAAAEADEKVQVALAGKTLRKFVFVPKRLVNFVAT
EEEEEEEECCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA