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The map label for this gene is thcD [H]

Identifier: 108761058

GI number: 108761058

Start: 5066197

End: 5067840

Strand: Direct

Name: thcD [H]

Synonym: MXAN_4126

Alternate gene names: 108761058

Gene position: 5066197-5067840 (Clockwise)

Preceding gene: 108759082

Following gene: 108759169

Centisome position: 55.43

GC content: 68.49

Gene sequence:

>1644_bases
GTGCCCATGAGAGTCGTCGTCGACCTCACCCGCTGCCAGGGGTATGCGCAGTGCGCCTTCCTTGCGCCGAGCGTGTTCAG
GATGCAGAGCGGGGATGCGCTGCTGTACGACTCCAACCCCGACGACGCCCTTCGTGAGCAGATCAAGCGAGCCGCTGCGG
CGTGCCCCGTCCAGGCCATCCACATTGGCGGGATGGGCACCCTGGCGGCGATGCGGAAAGAGCCTGCACAGCGACGGAAG
CCCAGGGGGCCCGTGCGTCTCACCGACGCGGAAGCGGCGTTCAAGCGCGGTGGCCGCATCGTTATCGTCGGCGCATCCCT
GGCCGGGTTGACTGCCGCCCTCATGCTCCGCACCCAAGGCTTTGCTGGGTCGTTGACCCTCATCGGGGATGAGGCCTGCG
ACCCGTACGACCGGCCGCCGCTCTCCAAGCAGGTCCTGACAGGTTGGGTGAAGGCAGACCACACCCTATTGCCTTACAGC
GACGAGCTCGATGCGGACTGGCACCTGGGAGTCCGTGCCATGGGACTGGACCTAGCCGGCAGGCAGGTCCTCCTTGCTGA
CGGGAACAGGGTCCAGTTCGACCGGTTGCTGATTGCGACGGGGGCGCGTGCCCGGCCGTGGCCCAACGACGCGGAGGCGT
CGCTGGAGGGCGTAGAGGTGGTGCGCACCCGCGACGATGCGGCCCAGTTGCAGCGCCGTCTGGCCGCCAGACCCGGCCGG
GTTCTGGTCATCGGCGGCGGGTTCACCGGCTCGGAGGTCGCCTCCGCGTGCCGCGAGCTGGGTCTGCCGGTGACCGTCAC
GGAGCGGGGGGCCACTCCACTGGTGGGGGCGCTCGGCGGAGTTGTCGGGGCGGTCGCAGCCGCCTTGCAACGCGACCATG
GCGTCGACCTGCGCTGCGGCGTCACCGTCACGCGGCTGGAGGGGGATGCAAACGGGAGGCTGCGGCGGGCCCACTTCTCC
GACGGCTCGACACTGAACGTCGACGTGGCCGTGGTCGCGCTAGGGGCGATCCGCAACGTGGAGTGGCTGCGCGACTCGGG
GCTGGCGGTGACTCGATGGGGAGTGGCCTGTGATGCGGCCTGCCACGCGTTCGACATCAACGGCCTGGTGACCAGGAACA
TCTTCGTCGCCGGGGACGTGGCCCGCGTCCCTCACCCGATCTACGACTACCAGTTCCTGTCCCTCGAGCACTGGGGCAAC
GCGGTCACCCAGGCCAAGATTGCGGCCCACAACATGCTCAGCCCGGAAGCGGACCGCTGGCCACACCTGACCATCCCCAC
CTTCTGGTCCACGCAATTCGGCGTCAACATCAAATCGGTGGGTGTGCCGACCTTCTCGGACGCGCTCGTCATCACCCAGG
GCTCACTCGCCGAGCGCCGATTCGTCGCCGTCTATGGCTACAAGGGGCGTGTCACCGCCGCGGTCGCTTTCGACCAGCCC
AAATGGCTGGAGTTCTACCAGGGCTTGATCGATGCAGCCGCGCCGTTCCCGCCCGCCTTCCGCACCGTCCATCAGCCCGC
CGAGATGCGGCCCGTCCCTCCCGAATTTCCGAAACGGGCCGTGTCCGAGCACGAGGCCACCGTCATGGTCACCGGCCGTG
AACCCTACGAGCGGCGGGTCCGGTGGGTGTACCGGCACCTTTGA

Upstream 100 bases:

>100_bases
TACGCCGCTCGCTGGACACCACCGGTCATTCCCTTCGAACTTCCGGCCCATTCCTCCCGTGCGCCTCCCATGCGAAATGC
CACCCCTTTGAGGAGGCCGT

Downstream 100 bases:

>100_bases
GCCGAATGTCGGCAGCCGCGTCACCCCACCCCTCCAACGGACTCAAGGAGACCGCTTCATGGCACAGGCCAGCATCTTCG
AGCAGATCCTCGACCCGGCC

Product: ferredoxin reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MPMRVVVDLTRCQGYAQCAFLAPSVFRMQSGDALLYDSNPDDALREQIKRAAAACPVQAIHIGGMGTLAAMRKEPAQRRK
PRGPVRLTDAEAAFKRGGRIVIVGASLAGLTAALMLRTQGFAGSLTLIGDEACDPYDRPPLSKQVLTGWVKADHTLLPYS
DELDADWHLGVRAMGLDLAGRQVLLADGNRVQFDRLLIATGARARPWPNDAEASLEGVEVVRTRDDAAQLQRRLAARPGR
VLVIGGGFTGSEVASACRELGLPVTVTERGATPLVGALGGVVGAVAAALQRDHGVDLRCGVTVTRLEGDANGRLRRAHFS
DGSTLNVDVAVVALGAIRNVEWLRDSGLAVTRWGVACDAACHAFDINGLVTRNIFVAGDVARVPHPIYDYQFLSLEHWGN
AVTQAKIAAHNMLSPEADRWPHLTIPTFWSTQFGVNIKSVGVPTFSDALVITQGSLAERRFVAVYGYKGRVTAAVAFDQP
KWLEFYQGLIDAAAPFPPAFRTVHQPAEMRPVPPEFPKRAVSEHEATVMVTGREPYERRVRWVYRHL

Sequences:

>Translated_547_residues
MPMRVVVDLTRCQGYAQCAFLAPSVFRMQSGDALLYDSNPDDALREQIKRAAAACPVQAIHIGGMGTLAAMRKEPAQRRK
PRGPVRLTDAEAAFKRGGRIVIVGASLAGLTAALMLRTQGFAGSLTLIGDEACDPYDRPPLSKQVLTGWVKADHTLLPYS
DELDADWHLGVRAMGLDLAGRQVLLADGNRVQFDRLLIATGARARPWPNDAEASLEGVEVVRTRDDAAQLQRRLAARPGR
VLVIGGGFTGSEVASACRELGLPVTVTERGATPLVGALGGVVGAVAAALQRDHGVDLRCGVTVTRLEGDANGRLRRAHFS
DGSTLNVDVAVVALGAIRNVEWLRDSGLAVTRWGVACDAACHAFDINGLVTRNIFVAGDVARVPHPIYDYQFLSLEHWGN
AVTQAKIAAHNMLSPEADRWPHLTIPTFWSTQFGVNIKSVGVPTFSDALVITQGSLAERRFVAVYGYKGRVTAAVAFDQP
KWLEFYQGLIDAAAPFPPAFRTVHQPAEMRPVPPEFPKRAVSEHEATVMVTGREPYERRVRWVYRHL
>Mature_546_residues
PMRVVVDLTRCQGYAQCAFLAPSVFRMQSGDALLYDSNPDDALREQIKRAAAACPVQAIHIGGMGTLAAMRKEPAQRRKP
RGPVRLTDAEAAFKRGGRIVIVGASLAGLTAALMLRTQGFAGSLTLIGDEACDPYDRPPLSKQVLTGWVKADHTLLPYSD
ELDADWHLGVRAMGLDLAGRQVLLADGNRVQFDRLLIATGARARPWPNDAEASLEGVEVVRTRDDAAQLQRRLAARPGRV
LVIGGGFTGSEVASACRELGLPVTVTERGATPLVGALGGVVGAVAAALQRDHGVDLRCGVTVTRLEGDANGRLRRAHFSD
GSTLNVDVAVVALGAIRNVEWLRDSGLAVTRWGVACDAACHAFDINGLVTRNIFVAGDVARVPHPIYDYQFLSLEHWGNA
VTQAKIAAHNMLSPEADRWPHLTIPTFWSTQFGVNIKSVGVPTFSDALVITQGSLAERRFVAVYGYKGRVTAAVAFDQPK
WLEFYQGLIDAAAPFPPAFRTVHQPAEMRPVPPEFPKRAVSEHEATVMVTGREPYERRVRWVYRHL

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=409, Percent_Identity=28.6063569682152, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI21389617, Length=409, Percent_Identity=28.6063569682152, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI65787454, Length=409, Percent_Identity=28.6063569682152, Blast_Score=152, Evalue=8e-37,
Organism=Escherichia coli, GI1788892, Length=406, Percent_Identity=31.5270935960591, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789065, Length=293, Percent_Identity=26.962457337884, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=341, Percent_Identity=26.099706744868, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI32564386, Length=353, Percent_Identity=27.4787535410765, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24639257, Length=429, Percent_Identity=25.6410256410256, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24585130, Length=373, Percent_Identity=27.3458445040214, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI281359715, Length=429, Percent_Identity=25.6410256410256, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI281359713, Length=429, Percent_Identity=25.6410256410256, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24639250, Length=429, Percent_Identity=25.6410256410256, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI18543267, Length=429, Percent_Identity=25.6410256410256, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24639252, Length=429, Percent_Identity=25.6410256410256, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI28573993, Length=370, Percent_Identity=27.027027027027, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24581020, Length=370, Percent_Identity=27.027027027027, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 59337; Mature: 59205

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMRVVVDLTRCQGYAQCAFLAPSVFRMQSGDALLYDSNPDDALREQIKRAAAACPVQAI
CCEEEEEEEHHCCCCHHHHHHCCHHEEECCCCEEEECCCCCHHHHHHHHHHHHHCCEEEE
HIGGMGTLAAMRKEPAQRRKPRGPVRLTDAEAAFKRGGRIVIVGASLAGLTAALMLRTQG
EECCCHHHHHHHCCHHHHCCCCCCEEEECHHHHHHCCCEEEEECCCHHHHHHHHHHHCCC
FAGSLTLIGDEACDPYDRPPLSKQVLTGWVKADHTLLPYSDELDADWHLGVRAMGLDLAG
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEEECCCC
RQVLLADGNRVQFDRLLIATGARARPWPNDAEASLEGVEVVRTRDDAAQLQRRLAARPGR
CEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCE
VLVIGGGFTGSEVASACRELGLPVTVTERGATPLVGALGGVVGAVAAALQRDHGVDLRCG
EEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEC
VTVTRLEGDANGRLRRAHFSDGSTLNVDVAVVALGAIRNVEWLRDSGLAVTRWGVACDAA
EEEEEEECCCCCCEEEEECCCCCEEEEEHHEEEHHHHHCHHHHHCCCCEEEECCCCHHCH
CHAFDINGLVTRNIFVAGDVARVPHPIYDYQFLSLEHWGNAVTQAKIAAHNMLSPEADRW
HEEEECCCEEEEEEEEECCHHHCCCCCCCEEEEEHHHHCCHHHHHHHHHHHCCCCCCCCC
PHLTIPTFWSTQFGVNIKSVGVPTFSDALVITQGSLAERRFVAVYGYKGRVTAAVAFDQP
CEEECCCCCCCCCCCEEEECCCCCCCCEEEEECCCCCCCEEEEEEECCCEEEEEEEECCC
KWLEFYQGLIDAAAPFPPAFRTVHQPAEMRPVPPEFPKRAVSEHEATVMVTGREPYERRV
HHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHH
RWVYRHL
HHHHHCC
>Mature Secondary Structure 
PMRVVVDLTRCQGYAQCAFLAPSVFRMQSGDALLYDSNPDDALREQIKRAAAACPVQAI
CEEEEEEEHHCCCCHHHHHHCCHHEEECCCCEEEECCCCCHHHHHHHHHHHHHCCEEEE
HIGGMGTLAAMRKEPAQRRKPRGPVRLTDAEAAFKRGGRIVIVGASLAGLTAALMLRTQG
EECCCHHHHHHHCCHHHHCCCCCCEEEECHHHHHHCCCEEEEECCCHHHHHHHHHHHCCC
FAGSLTLIGDEACDPYDRPPLSKQVLTGWVKADHTLLPYSDELDADWHLGVRAMGLDLAG
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEEECCCC
RQVLLADGNRVQFDRLLIATGARARPWPNDAEASLEGVEVVRTRDDAAQLQRRLAARPGR
CEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCE
VLVIGGGFTGSEVASACRELGLPVTVTERGATPLVGALGGVVGAVAAALQRDHGVDLRCG
EEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEC
VTVTRLEGDANGRLRRAHFSDGSTLNVDVAVVALGAIRNVEWLRDSGLAVTRWGVACDAA
EEEEEEECCCCCCEEEEECCCCCEEEEEHHEEEHHHHHCHHHHHCCCCEEEECCCCHHCH
CHAFDINGLVTRNIFVAGDVARVPHPIYDYQFLSLEHWGNAVTQAKIAAHNMLSPEADRW
HEEEECCCEEEEEEEEECCHHHCCCCCCCEEEEEHHHHCCHHHHHHHHHHHCCCCCCCCC
PHLTIPTFWSTQFGVNIKSVGVPTFSDALVITQGSLAERRFVAVYGYKGRVTAAVAFDQP
CEEECCCCCCCCCCCEEEECCCCCCCCEEEEECCCCCCCEEEEEEECCCEEEEEEEECCC
KWLEFYQGLIDAAAPFPPAFRTVHQPAEMRPVPPEFPKRAVSEHEATVMVTGREPYERRV
HHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHH
RWVYRHL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]