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The map label for this gene is mhpC [H]

Identifier: 108761042

GI number: 108761042

Start: 267544

End: 268734

Strand: Direct

Name: mhpC [H]

Synonym: MXAN_0220

Alternate gene names: 108761042

Gene position: 267544-268734 (Clockwise)

Preceding gene: 108757512

Following gene: 108758562

Centisome position: 2.93

GC content: 69.86

Gene sequence:

>1191_bases
ATGAGCACCTCCCGCGCATTCACGGCCCTGCTCCTCGCGGCGGGGCTCTCCTCCGCCGGTTGCGTGCGCTCGTACGCGCA
CCAGCCCGCGCTCTCCTTCCAGGATTTGGACTACACCTCCGAGGGCTCGAAGCAGCCCTGGCCGGTGAAACGGATTCCGC
TTCCGCGCACCGCGAATCAGTACGGCATGGCCGTGGTGCCACAGATTGCCTACGTCGACATGCCCGGCAGCGGCCCGGAC
GCGAAGGCCGTCGTCTTCATCCACGGCCTGGGTTCGTACCTGAAGTTCTGGCGGGCGCAGCTGGACGCCTTCCAGAAGCA
GGGCTACCGCGTCATCGCGGTGGACCTGCCCGGCTACGGCAAGTCCGACAAGCCCGGCACCTTCCCGTACACCATGGAGG
CCATGGCGGACGCGGTGCTGGAGTTGGTGGACGGCCTGGGCCTGGACAAGCCCGTGCTCGCCGGTCACTCCATGGGCGGG
CAGACGTCGCTCTCCTTCGCCATCCGCTACCCGGAGTCGCTGAGCGGGCTGGTGCTGGCGTCGCCCGCCGGCTTCGAGAA
GTTCAGCTGGCGGGAGAAGGAGTGGTTCGCGCGGGCGATGAGCTCCGAGTTCATCAAGTCCGCGCCGGAGGCCAGCATCT
GGGGCAGCGTGCGCCAGGCCAACTTCATGCACTGGCGGCCGGAGTTGGAGTGGCTGATTGAGGAGCGCGTGCGGCTGGCG
AAGTCGCCCGAGTTCGACGCCTACGCCTACGCCAACGTGCGCACGGTGCGAGGCCTGTCCCACAACGACTTCGTGCGCGA
CAACCTGCACCGCGTCACCGTGCCCACCGTCATCATCTACGGCACGGATGACCGGCTCATCCCCAGCCCCTTCCTCCACG
GCGGAGAGACGCGCGGCATCATGGAGTACGGCGCGTCCCACATTCCCGGCGCGAAGCTGGTGGCGCTGGAGGGCTGTGGG
CACACCGTGCAGCTCGACTGTGCGGAGCGCTTCAACGAGGCGGCCTTCGCCTTCGTCCGTGACGCGGCGGCGGGGCGCAT
CGAGGTGCCGTCCGCTCCCGCGAAGGAGGAGGCGCCGCGGCCGGAGTCTCCTCCCGCGCCGGCGTCCGAGCCGCTCACGC
CGGAGAGCCTGCCTCCGCCCACGCCGGGCCAGGAAGGCGAGCCGACGCCGGGCGTGCTGCCCCAGCCGTAG

Upstream 100 bases:

>100_bases
CGGGCTACCTGTTCCGGGGCAGCTTCTACGACGGCTCGCCCACCATCCGGACCAATCCCTTCTCCGTCTTCACCACCTTC
ACCTGGTACGCGTTCTGACC

Downstream 100 bases:

>100_bases
TGGGGTGGTGAGCCGGTCCACCTCCACCCTTTCGCGAGGGTGGAGGTGCCGGGTGCGGATGACTACGGGATGGTGTGGTT
GACCACCATGTCGTACTGAA

Product: alpha/beta fold family hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSTSRAFTALLLAAGLSSAGCVRSYAHQPALSFQDLDYTSEGSKQPWPVKRIPLPRTANQYGMAVVPQIAYVDMPGSGPD
AKAVVFIHGLGSYLKFWRAQLDAFQKQGYRVIAVDLPGYGKSDKPGTFPYTMEAMADAVLELVDGLGLDKPVLAGHSMGG
QTSLSFAIRYPESLSGLVLASPAGFEKFSWREKEWFARAMSSEFIKSAPEASIWGSVRQANFMHWRPELEWLIEERVRLA
KSPEFDAYAYANVRTVRGLSHNDFVRDNLHRVTVPTVIIYGTDDRLIPSPFLHGGETRGIMEYGASHIPGAKLVALEGCG
HTVQLDCAERFNEAAFAFVRDAAAGRIEVPSAPAKEEAPRPESPPAPASEPLTPESLPPPTPGQEGEPTPGVLPQP

Sequences:

>Translated_396_residues
MSTSRAFTALLLAAGLSSAGCVRSYAHQPALSFQDLDYTSEGSKQPWPVKRIPLPRTANQYGMAVVPQIAYVDMPGSGPD
AKAVVFIHGLGSYLKFWRAQLDAFQKQGYRVIAVDLPGYGKSDKPGTFPYTMEAMADAVLELVDGLGLDKPVLAGHSMGG
QTSLSFAIRYPESLSGLVLASPAGFEKFSWREKEWFARAMSSEFIKSAPEASIWGSVRQANFMHWRPELEWLIEERVRLA
KSPEFDAYAYANVRTVRGLSHNDFVRDNLHRVTVPTVIIYGTDDRLIPSPFLHGGETRGIMEYGASHIPGAKLVALEGCG
HTVQLDCAERFNEAAFAFVRDAAAGRIEVPSAPAKEEAPRPESPPAPASEPLTPESLPPPTPGQEGEPTPGVLPQP
>Mature_395_residues
STSRAFTALLLAAGLSSAGCVRSYAHQPALSFQDLDYTSEGSKQPWPVKRIPLPRTANQYGMAVVPQIAYVDMPGSGPDA
KAVVFIHGLGSYLKFWRAQLDAFQKQGYRVIAVDLPGYGKSDKPGTFPYTMEAMADAVLELVDGLGLDKPVLAGHSMGGQ
TSLSFAIRYPESLSGLVLASPAGFEKFSWREKEWFARAMSSEFIKSAPEASIWGSVRQANFMHWRPELEWLIEERVRLAK
SPEFDAYAYANVRTVRGLSHNDFVRDNLHRVTVPTVIIYGTDDRLIPSPFLHGGETRGIMEYGASHIPGAKLVALEGCGH
TVQLDCAERFNEAAFAFVRDAAAGRIEVPSAPAKEEAPRPESPPAPASEPLTPESLPPPTPGQEGEPTPGVLPQP

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mhpC alpha/beta hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI27597073, Length=133, Percent_Identity=35.3383458646617, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI189027141, Length=263, Percent_Identity=24.7148288973384, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI218777837, Length=294, Percent_Identity=23.469387755102, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI218777840, Length=285, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI13376164, Length=285, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI87081721, Length=291, Percent_Identity=26.4604810996564, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787244, Length=238, Percent_Identity=26.890756302521, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25146278, Length=297, Percent_Identity=30.3030303030303, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32566936, Length=314, Percent_Identity=27.3885350318471, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17558492, Length=314, Percent_Identity=27.3885350318471, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17554012, Length=139, Percent_Identity=33.0935251798561, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24586387, Length=331, Percent_Identity=24.4712990936556, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24586391, Length=331, Percent_Identity=24.4712990936556, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24586389, Length=331, Percent_Identity=24.4712990936556, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24586385, Length=331, Percent_Identity=24.4712990936556, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 43052; Mature: 42921

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00380 RHODANESE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSRAFTALLLAAGLSSAGCVRSYAHQPALSFQDLDYTSEGSKQPWPVKRIPLPRTANQ
CCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YGMAVVPQIAYVDMPGSGPDAKAVVFIHGLGSYLKFWRAQLDAFQKQGYRVIAVDLPGYG
CCCEEECEEEEEECCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
KSDKPGTFPYTMEAMADAVLELVDGLGLDKPVLAGHSMGGQTSLSFAIRYPESLSGLVLA
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCEEEEEEECCCCCCCEEEE
SPAGFEKFSWREKEWFARAMSSEFIKSAPEASIWGSVRQANFMHWRPELEWLIEERVRLA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCHHHHHHHHHHHHHH
KSPEFDAYAYANVRTVRGLSHNDFVRDNLHRVTVPTVIIYGTDDRLIPSPFLHGGETRGI
CCCCCCEEEEECCCEECCCCCCCHHHHCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCH
MEYGASHIPGAKLVALEGCGHTVQLDCAERFNEAAFAFVRDAAAGRIEVPSAPAKEEAPR
HHHCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCC
PESPPAPASEPLTPESLPPPTPGQEGEPTPGVLPQP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STSRAFTALLLAAGLSSAGCVRSYAHQPALSFQDLDYTSEGSKQPWPVKRIPLPRTANQ
CCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YGMAVVPQIAYVDMPGSGPDAKAVVFIHGLGSYLKFWRAQLDAFQKQGYRVIAVDLPGYG
CCCEEECEEEEEECCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
KSDKPGTFPYTMEAMADAVLELVDGLGLDKPVLAGHSMGGQTSLSFAIRYPESLSGLVLA
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCEEEEEEECCCCCCCEEEE
SPAGFEKFSWREKEWFARAMSSEFIKSAPEASIWGSVRQANFMHWRPELEWLIEERVRLA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCHHHHHHHHHHHHHH
KSPEFDAYAYANVRTVRGLSHNDFVRDNLHRVTVPTVIIYGTDDRLIPSPFLHGGETRGI
CCCCCCEEEEECCCEECCCCCCCHHHHCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCH
MEYGASHIPGAKLVALEGCGHTVQLDCAERFNEAAFAFVRDAAAGRIEVPSAPAKEEAPR
HHHCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCC
PESPPAPASEPLTPESLPPPTPGQEGEPTPGVLPQP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA