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The map label for this gene is pcnB [H]

Identifier: 108761014

GI number: 108761014

Start: 2961744

End: 2963390

Strand: Direct

Name: pcnB [H]

Synonym: MXAN_2545

Alternate gene names: 108761014

Gene position: 2961744-2963390 (Clockwise)

Preceding gene: 108764040

Following gene: 108759792

Centisome position: 32.41

GC content: 71.65

Gene sequence:

>1647_bases
GTGGCCGCCGACGAGCAGGCCCCCGCTGAAATCCCCTCGATACAGGTCGCCACCCCCGCCGAGCCGCCTCCGGCCCCCGC
TGACGCGGATGCCGAGGACGACGACGACGATGAGGAAGATGACGACGAGGTAGACCCGGCCGAGTCGGGCCTGGACGACG
CCGTCCTGGGCGCCGCCGAGGCCGCGCTGGCCGCCGCCGAGGCCGAGGACGCCGCCGAGGCCCGGGGCGAGGACGCCGAG
GAAGTCCCCATCGTCCTGGAGCCCGAACCGGAGCCGACGCCCGATGAGCGCGCGCTGCACGCGCCCCATGTCCGGCCCAG
CGGCGAGCCGGCGGAAATCGACCCCGATGAGCTGGACCCGGACGCCCTCAAGGTGGTGCTCCGCCTGCACCAGCACGGCC
ATCAGGCGTACATGGTGGGCGGCTGCGTGCGTGACCTGCTGCTGGGGCGCAAGCCGAAGGACTTCGACATCGCCACCAGC
GCGACGCCGAACGAGGTGCGCGGCATCTTCCGCAACTGCCGCCTGATTGGCCGGCGCTTCCGGCTGGCGCACGTCTACTT
CAAAGGCGGGAAGATCATCGAGGTCTCCACCTTCCGTGCGAATCCGACGGAGCTGGAGCACGCGGCCAACGGCGGTGAGG
ATGAGGGGGACGGTGAGGACCTGCTCATCACCCACGACAACGTCTTCGGCACCGCGCAGCAGGACGCGCGCCGCCGTGAC
TTCACCATCAACGGCCTGTTCTACGACGTGGCCGAAGGGCGGGTCATCGATTACGTCCGGGGCCGCCGTGACCTGGATGA
GCGCTTCATCCGCACCATTGGCGACCCGGAAGTGCGCATGCGCGAGGACCCGGTGCGCATCCTGCGCGCGGTGCGCTTCG
CGGCGAAGCTGGGCCTGGACATCGAGTCGCGGACGTACGCGGCCATGGAAGGCGCGGTGGAGGACCTGCCCCGCTGCGCG
CCCGCGCGCCTGCTGGAGGAGACGTTCCGCCTCATCCGCGGCGGTGTGTCCGCGCCGGCGCTGAAGCTGCTGGACGCGCT
GGACGCGCTCAAAATCCTCCTGCCGCCCGTCAACGCGTACCTCAAGCAGCACGGCAAGGAAGGGGAGAAGACGTTCTACG
CCTTCGCGGAGTCGCTGGACCGGCGCGTGTCCGCGGGCGAGGCGCTGGACGACGCCATCCTCCTGGCGATGTTGCTGATT
CCCATCAGCCGCTCGACGGGGCCCGAGGAGTCGCAGGAGGGCCGCCCGTCCGTGTCGCAGGTGGTGGAAGACCTGCTCGC
GGGCTTCGTCCAGTCCGCGCGTCTACCGCGCCGCATCGCCGAGCGTTGCCGCATGCTGCTGCTGGCCCAGCGCACCCTGT
CCGGTGAGCGCCGTCGTCGCAGCGCGGCCTTCAAGCGGCACCCGCTCTTCAGCGAGGCCCTCACCGTGTTCGAGATGACG
GTGGAGGCCACGGGCGAGAATCGCGAGCAGTTGGAGGCGTGGAAGGCCGGCGAGGTGCCGCAGCCGCGTGCCGCCGCCGC
GGACGGCGAGGAGTCCGACGCGGGAGGCCAGCGCAAGCGCCGCCGCCGCCGCCGCCGCCGCCGTCCCTCGGCGAACGGCT
CGTCCGGTGAGAGCGCCGATTCGTCCTCCGAGTCGGGCGACGCCTGA

Upstream 100 bases:

>100_bases
CGAGGCGGCAGGGACTTCCGAGCGCGGCGAACAAGAGGTTCCCATGAACTCCCAACTGGAGCTGTCGGCGCACCCCGAAG
AGGACAGCGCCTCCAAGCCC

Downstream 100 bases:

>100_bases
CTCGAGGTCGCGTCCGTGATGTGAGGGCACCGGCTCGTCTTCCGGGGCGGGCGCTGCCTGAGTCGAAGTCCCTTCCGTGA
GGTGTGGGGCCGCTCCGCGA

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MAADEQAPAEIPSIQVATPAEPPPAPADADAEDDDDDEEDDDEVDPAESGLDDAVLGAAEAALAAAEAEDAAEARGEDAE
EVPIVLEPEPEPTPDERALHAPHVRPSGEPAEIDPDELDPDALKVVLRLHQHGHQAYMVGGCVRDLLLGRKPKDFDIATS
ATPNEVRGIFRNCRLIGRRFRLAHVYFKGGKIIEVSTFRANPTELEHAANGGEDEGDGEDLLITHDNVFGTAQQDARRRD
FTINGLFYDVAEGRVIDYVRGRRDLDERFIRTIGDPEVRMREDPVRILRAVRFAAKLGLDIESRTYAAMEGAVEDLPRCA
PARLLEETFRLIRGGVSAPALKLLDALDALKILLPPVNAYLKQHGKEGEKTFYAFAESLDRRVSAGEALDDAILLAMLLI
PISRSTGPEESQEGRPSVSQVVEDLLAGFVQSARLPRRIAERCRMLLLAQRTLSGERRRRSAAFKRHPLFSEALTVFEMT
VEATGENREQLEAWKAGEVPQPRAAAADGEESDAGGQRKRRRRRRRRRPSANGSSGESADSSSESGDA

Sequences:

>Translated_548_residues
MAADEQAPAEIPSIQVATPAEPPPAPADADAEDDDDDEEDDDEVDPAESGLDDAVLGAAEAALAAAEAEDAAEARGEDAE
EVPIVLEPEPEPTPDERALHAPHVRPSGEPAEIDPDELDPDALKVVLRLHQHGHQAYMVGGCVRDLLLGRKPKDFDIATS
ATPNEVRGIFRNCRLIGRRFRLAHVYFKGGKIIEVSTFRANPTELEHAANGGEDEGDGEDLLITHDNVFGTAQQDARRRD
FTINGLFYDVAEGRVIDYVRGRRDLDERFIRTIGDPEVRMREDPVRILRAVRFAAKLGLDIESRTYAAMEGAVEDLPRCA
PARLLEETFRLIRGGVSAPALKLLDALDALKILLPPVNAYLKQHGKEGEKTFYAFAESLDRRVSAGEALDDAILLAMLLI
PISRSTGPEESQEGRPSVSQVVEDLLAGFVQSARLPRRIAERCRMLLLAQRTLSGERRRRSAAFKRHPLFSEALTVFEMT
VEATGENREQLEAWKAGEVPQPRAAAADGEESDAGGQRKRRRRRRRRRPSANGSSGESADSSSESGDA
>Mature_547_residues
AADEQAPAEIPSIQVATPAEPPPAPADADAEDDDDDEEDDDEVDPAESGLDDAVLGAAEAALAAAEAEDAAEARGEDAEE
VPIVLEPEPEPTPDERALHAPHVRPSGEPAEIDPDELDPDALKVVLRLHQHGHQAYMVGGCVRDLLLGRKPKDFDIATSA
TPNEVRGIFRNCRLIGRRFRLAHVYFKGGKIIEVSTFRANPTELEHAANGGEDEGDGEDLLITHDNVFGTAQQDARRRDF
TINGLFYDVAEGRVIDYVRGRRDLDERFIRTIGDPEVRMREDPVRILRAVRFAAKLGLDIESRTYAAMEGAVEDLPRCAP
ARLLEETFRLIRGGVSAPALKLLDALDALKILLPPVNAYLKQHGKEGEKTFYAFAESLDRRVSAGEALDDAILLAMLLIP
ISRSTGPEESQEGRPSVSQVVEDLLAGFVQSARLPRRIAERCRMLLLAQRTLSGERRRRSAAFKRHPLFSEALTVFEMTV
EATGENREQLEAWKAGEVPQPRAAAADGEESDAGGQRKRRRRRRRRRPSANGSSGESADSSSESGDA

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=248, Percent_Identity=30.241935483871, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87081691, Length=410, Percent_Identity=38.2926829268293, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1789436, Length=234, Percent_Identity=30.3418803418803, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71995920, Length=227, Percent_Identity=29.9559471365639, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321016, Length=185, Percent_Identity=30.2702702702703, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281360112, Length=252, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21357337, Length=252, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 60024; Mature: 59893

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADEQAPAEIPSIQVATPAEPPPAPADADAEDDDDDEEDDDEVDPAESGLDDAVLGAAE
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHH
AALAAAEAEDAAEARGEDAEEVPIVLEPEPEPTPDERALHAPHVRPSGEPAEIDPDELDP
HHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
DALKVVLRLHQHGHQAYMVGGCVRDLLLGRKPKDFDIATSATPNEVRGIFRNCRLIGRRF
HHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RLAHVYFKGGKIIEVSTFRANPTELEHAANGGEDEGDGEDLLITHDNVFGTAQQDARRRD
HEEEEEECCCEEEEEEECCCCHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHHHCC
FTINGLFYDVAEGRVIDYVRGRRDLDERFIRTIGDPEVRMREDPVRILRAVRFAAKLGLD
EEECCEEEECCCCCHHHHHHCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHCCC
IESRTYAAMEGAVEDLPRCAPARLLEETFRLIRGGVSAPALKLLDALDALKILLPPVNAY
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHH
LKQHGKEGEKTFYAFAESLDRRVSAGEALDDAILLAMLLIPISRSTGPEESQEGRPSVSQ
HHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
VVEDLLAGFVQSARLPRRIAERCRMLLLAQRTLSGERRRRSAAFKRHPLFSEALTVFEMT
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VEATGENREQLEAWKAGEVPQPRAAAADGEESDAGGQRKRRRRRRRRRPSANGSSGESAD
HHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
SSSESGDA
CCCCCCCC
>Mature Secondary Structure 
AADEQAPAEIPSIQVATPAEPPPAPADADAEDDDDDEEDDDEVDPAESGLDDAVLGAAE
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHH
AALAAAEAEDAAEARGEDAEEVPIVLEPEPEPTPDERALHAPHVRPSGEPAEIDPDELDP
HHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
DALKVVLRLHQHGHQAYMVGGCVRDLLLGRKPKDFDIATSATPNEVRGIFRNCRLIGRRF
HHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RLAHVYFKGGKIIEVSTFRANPTELEHAANGGEDEGDGEDLLITHDNVFGTAQQDARRRD
HEEEEEECCCEEEEEEECCCCHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHHHCC
FTINGLFYDVAEGRVIDYVRGRRDLDERFIRTIGDPEVRMREDPVRILRAVRFAAKLGLD
EEECCEEEECCCCCHHHHHHCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHCCC
IESRTYAAMEGAVEDLPRCAPARLLEETFRLIRGGVSAPALKLLDALDALKILLPPVNAY
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHH
LKQHGKEGEKTFYAFAESLDRRVSAGEALDDAILLAMLLIPISRSTGPEESQEGRPSVSQ
HHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
VVEDLLAGFVQSARLPRRIAERCRMLLLAQRTLSGERRRRSAAFKRHPLFSEALTVFEMT
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VEATGENREQLEAWKAGEVPQPRAAAADGEESDAGGQRKRRRRRRRRRPSANGSSGESAD
HHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
SSSESGDA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]