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The map label for this gene is cimA [H]

Identifier: 108760628

GI number: 108760628

Start: 1500306

End: 1501571

Strand: Direct

Name: cimA [H]

Synonym: MXAN_1284

Alternate gene names: 108760628

Gene position: 1500306-1501571 (Clockwise)

Preceding gene: 108763358

Following gene: 108761582

Centisome position: 16.42

GC content: 66.67

Gene sequence:

>1266_bases
ATGCGCGACCCGGCCAGGACTGTTTCTCATTCCGAGTTCATCCACGACTGGAATGGAAGGGGGGCCGCCCCGAGCCCTGC
ATTCCCAGTCGAGATCAACGATGAGACGCTCCGGGATGGGATGCAGGCGACGGCCGTCACCAGCCCCACGGTGGAAGAGA
AGCGCGGCCTGCTCGAGTTGATGCAACACATCGGGGTGAGCGCGGTGAGCCTCGGTATGCCCGCGGCGGGGCCGTGGGCG
CTCGAGGAGGTCGTGACGCTGGCGCGCCACATCCGGGAGGAGGCGCTGCGGCTCGAGCCCAACTGTGCGGCGCGGACGCT
GCACAGCGACATCGTCCCCATTGTCGAGGCGGTGCAGCGGGCGGGGCAGCCCATCGTGGTGCATACGTTCATTGGCTCGT
CCCCCCTACGCCAGTGCGCTGAGGCATGGGACCTGGACTTCATTCTGCGTTCCTCGACGGCGGCCATCGACTTCGCCGTG
CGTCAGGGGCTGGAGGTCGCGTTCATCACCGAGGACACCACCCGCTCCACGCCGGAGACGCTCGAGCTCCTCTTCCGCGC
CGCGGTGGAGCATGGCGCTTCCCGGCTGGTGCTCTGCGACACCGTGGGGCATGCGACGCCAGCCGGAACCCGCGCCCTGG
TGCGGTGGACACGGGACCTGGTGGAGCGGCTCGGCAGTCCGGTGAAGGTCGAGTGGCATGGTCACAACGACCGAGGGTTG
GCGTTGATCAACGCGCTCGTGGCCCTGGAGGCGGGAGCGACGCGAGTCCATGGTTGTGGGCTGGGAATCGGCGAGCGCAC
GGGCAATGCCCCCGTGGAGTTGCTGCTGCTCAATCTCAAGCTCCTGGGGTGGATTGACCAGGACCTCACGCGGCTCGTGG
ACTACGTCCTCACGCTTGCCCAGGTCTGCCGGGTTCCGATTCCGCGGAACTATCCGCTGGTGGGGGAGGATGCGTTTCGC
ACTGCCACGGGCGTTCATTCGGCCGCCATCATCAAGGCGCTCGAGCGGGGGGATGATTGGCTTGCCGACCACATCTATTC
ATCCGTTCCCGCTGGTGAGTTTGGCCGGCGCCAGCAGCTCGAACTCGGGCCGATGAGCGGGATGAGCGGCGTCAGGTATT
GGCTGATGGTGCGTGGCCTGCAAGAGAATGAGCAGCTCTGCGAGGAGATCCTCCGGAGGGCCAAGTCCGCGGCGTCGACA
CTGACGGAAGAAGAAGTGTGGTCAATCGTGCAGGCGCACGGTGGTCAGGGGCGCTCCTGTGCGTGA

Upstream 100 bases:

>100_bases
GGGTTCTGGTCTTCCGGTTGGGGGTGGTCTTTTCTTGGTGCCAGGATTTGTTGCCGCGAACCCGCAGGTCATTCGAAACC
GCTGAGGGCAAGGCATTTCC

Downstream 100 bases:

>100_bases
GCGCTCTTGTTTCCGGTGCGCCCTCTTCAATCCAATGGATGTGTTCGGTGCATGGAGTTGCTCATGAGCGGAGCCTGGGA
AGGCAAGCTGTATGATGTGA

Product: 2-isopropylmalate synthase/homocitrate synthase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MRDPARTVSHSEFIHDWNGRGAAPSPAFPVEINDETLRDGMQATAVTSPTVEEKRGLLELMQHIGVSAVSLGMPAAGPWA
LEEVVTLARHIREEALRLEPNCAARTLHSDIVPIVEAVQRAGQPIVVHTFIGSSPLRQCAEAWDLDFILRSSTAAIDFAV
RQGLEVAFITEDTTRSTPETLELLFRAAVEHGASRLVLCDTVGHATPAGTRALVRWTRDLVERLGSPVKVEWHGHNDRGL
ALINALVALEAGATRVHGCGLGIGERTGNAPVELLLLNLKLLGWIDQDLTRLVDYVLTLAQVCRVPIPRNYPLVGEDAFR
TATGVHSAAIIKALERGDDWLADHIYSSVPAGEFGRRQQLELGPMSGMSGVRYWLMVRGLQENEQLCEEILRRAKSAAST
LTEEEVWSIVQAHGGQGRSCA

Sequences:

>Translated_421_residues
MRDPARTVSHSEFIHDWNGRGAAPSPAFPVEINDETLRDGMQATAVTSPTVEEKRGLLELMQHIGVSAVSLGMPAAGPWA
LEEVVTLARHIREEALRLEPNCAARTLHSDIVPIVEAVQRAGQPIVVHTFIGSSPLRQCAEAWDLDFILRSSTAAIDFAV
RQGLEVAFITEDTTRSTPETLELLFRAAVEHGASRLVLCDTVGHATPAGTRALVRWTRDLVERLGSPVKVEWHGHNDRGL
ALINALVALEAGATRVHGCGLGIGERTGNAPVELLLLNLKLLGWIDQDLTRLVDYVLTLAQVCRVPIPRNYPLVGEDAFR
TATGVHSAAIIKALERGDDWLADHIYSSVPAGEFGRRQQLELGPMSGMSGVRYWLMVRGLQENEQLCEEILRRAKSAAST
LTEEEVWSIVQAHGGQGRSCA
>Mature_421_residues
MRDPARTVSHSEFIHDWNGRGAAPSPAFPVEINDETLRDGMQATAVTSPTVEEKRGLLELMQHIGVSAVSLGMPAAGPWA
LEEVVTLARHIREEALRLEPNCAARTLHSDIVPIVEAVQRAGQPIVVHTFIGSSPLRQCAEAWDLDFILRSSTAAIDFAV
RQGLEVAFITEDTTRSTPETLELLFRAAVEHGASRLVLCDTVGHATPAGTRALVRWTRDLVERLGSPVKVEWHGHNDRGL
ALINALVALEAGATRVHGCGLGIGERTGNAPVELLLLNLKLLGWIDQDLTRLVDYVLTLAQVCRVPIPRNYPLVGEDAFR
TATGVHSAAIIKALERGDDWLADHIYSSVPAGEFGRRQQLELGPMSGMSGVRYWLMVRGLQENEQLCEEILRRAKSAAST
LTEEEVWSIVQAHGGQGRSCA

Specific function: Catalyzes the condensation of pyruvate and acetyl- coenzyme A to form (R)-citramalate [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=366, Percent_Identity=30.0546448087432, Blast_Score=141, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6320019, Length=386, Percent_Identity=25.6476683937824, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6320071, Length=386, Percent_Identity=25.6476683937824, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.1.182 [H]

Molecular weight: Translated: 45912; Mature: 45912

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDPARTVSHSEFIHDWNGRGAAPSPAFPVEINDETLRDGMQATAVTSPTVEEKRGLLEL
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHCCCHHHCCCCCCHHHHHHHHHH
MQHIGVSAVSLGMPAAGPWALEEVVTLARHIREEALRLEPNCAARTLHSDIVPIVEAVQR
HHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AGQPIVVHTFIGSSPLRQCAEAWDLDFILRSSTAAIDFAVRQGLEVAFITEDTTRSTPET
CCCCEEEEEECCCHHHHHHHHHCCHHHHHHCCCHHHHHHHHCCCEEEEEECCCCCCCHHH
LELLFRAAVEHGASRLVLCDTVGHATPAGTRALVRWTRDLVERLGSPVKVEWHGHNDRGL
HHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
ALINALVALEAGATRVHGCGLGIGERTGNAPVELLLLNLKLLGWIDQDLTRLVDYVLTLA
HHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVCRVPIPRNYPLVGEDAFRTATGVHSAAIIKALERGDDWLADHIYSSVPAGEFGRRQQL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCC
ELGPMSGMSGVRYWLMVRGLQENEQLCEEILRRAKSAASTLTEEEVWSIVQAHGGQGRSC
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
A
C
>Mature Secondary Structure
MRDPARTVSHSEFIHDWNGRGAAPSPAFPVEINDETLRDGMQATAVTSPTVEEKRGLLEL
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHCCCHHHCCCCCCHHHHHHHHHH
MQHIGVSAVSLGMPAAGPWALEEVVTLARHIREEALRLEPNCAARTLHSDIVPIVEAVQR
HHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AGQPIVVHTFIGSSPLRQCAEAWDLDFILRSSTAAIDFAVRQGLEVAFITEDTTRSTPET
CCCCEEEEEECCCHHHHHHHHHCCHHHHHHCCCHHHHHHHHCCCEEEEEECCCCCCCHHH
LELLFRAAVEHGASRLVLCDTVGHATPAGTRALVRWTRDLVERLGSPVKVEWHGHNDRGL
HHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
ALINALVALEAGATRVHGCGLGIGERTGNAPVELLLLNLKLLGWIDQDLTRLVDYVLTLA
HHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVCRVPIPRNYPLVGEDAFRTATGVHSAAIIKALERGDDWLADHIYSSVPAGEFGRRQQL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCC
ELGPMSGMSGVRYWLMVRGLQENEQLCEEILRRAKSAASTLTEEEVWSIVQAHGGQGRSC
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]