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The map label for this gene is poxB [H]

Identifier: 108760514

GI number: 108760514

Start: 4635521

End: 4637314

Strand: Reverse

Name: poxB [H]

Synonym: MXAN_3857

Alternate gene names: 108760514

Gene position: 4637314-4635521 (Counterclockwise)

Preceding gene: 108758266

Following gene: 108757367

Centisome position: 50.74

GC content: 67.17

Gene sequence:

>1794_bases
ATGAGCGCCACCGTCTCCGATTACCTGCTCCACCGCCTGTCCCAGTGGGGCGTCCGCCGCATCTATGGCTATCCCGGTGA
CGGCATCAACGGCATCCTCGGCGCGCTCGGGCGCACGTTGGAGTTCCGCTTCGTGCAGACGCGCCATGAAGAGATGTCCG
CGTTCATGGCCTGTGCGCACGCGAAGTTCACCGGCGAGCCGGGCGTGTGTCTGGCCACGTCGGGGCCCGGGGCCATCCAT
CTGCTCAATGGCCTCTATGACGCCAAGCTGGACCACCAGCCGGTGGTGGCCATCGTCGGACAGCAGACGCGCTCAGCGCT
GGGCGGCCACTTCCAGCAGGAGGTGGATCTGATGTCCCTCTTCAAGGACGTGGCCAGCGAATACCTCACGATGGTGACCA
GCCCCTCCGCCATCCGCCACGCGGTGGACCGGGCGATGCGCGTCGCCATCTACGAGCGCACCGTGACGTGCGTCATCATC
CCCAACGACGTCCAGGAAGAGGCCTACCACAAGCCTCCGCACGCGCACGGCACGGTCCATTCAAGCGTGGGCTACAGCGT
GCCGCGAGTGCTGCCTCGCGAGCGGGACTTGCGGCGGGCGGCGGACGTGCTCAACGCCGGGAAGAAGGTGGCCATGCTGG
TGGGCGCCGGAGCGCAGGCCGCGGCGGACGAGGTGATGCACGTGGCGGAGTTGCTAGGCGCGGGTGTGGCGAAGGCCCTG
CTGGGCAAGGCCGTGCTGCCGGATGACGTTCCCTATGTCACCGGCTCCATCGGGCTGCTGGGCACCAAGGCGAGCTGGGA
CCTGATGTCGGACTGCGACACGTTGCTGATGGTGGGCACCAGCTTTCCGTACTCGGAGTTCCTCCCGAAGGAGGGACAGG
CCCGGGGCGTTCAAATCGACCTCGATGGCCGGATGCTGGCCATCCGCTACCCCATGGAGGTGGCGCTGGTGGGGGACAGT
CAGGAGACATTGAAGGCGCTGATTCCCATGCTCGAGCACAAGAAGGACCGGAGCTGGCGCGACAGCGTGGTGAAGAACGT
GCGGCAGTGGGAGAAGACGGCGGAGGCGCTCGCGATGGACCGCGCGAATCCCGTCAACCCCCGGCGCGTGTTCTTCGAAC
TGTCGTCACGGCTGCCGGATGGCGTCATCCTGACGGCGGATTCGGGGTCGGGCACGAGCTGGTACGCGCAGCACCTGAAG
ATGCGCAAGGGGATGATGGCCTCGCTGTCCGGGAACCTGGCCAGCATGGGCTGCGGCGTGCCCTATGCCATCGGCGCCAA
GTTCGCCTTCCCGCACCGGCCCGTCATCGCGCTGGTGGGCGACGGTGCCATGCAGATGAATGGCAACGCGGAGCTCATCA
CCGTGGCCAAGTACTGGAAGGAGTGGAAGGACCCGCGCTTCATCGTCATGGTCCTCAACAACCGGGACCTCAACATGGTG
ACGTGGGAGCAGCGCGTGCTGGCCGGCGACCCGAAGCTGCCCGCGTCCCAGGACCTGCCGGACTTCCCGTACGCGGCCTA
TGCGGAGTCCCTGGGCTTCCTGGGCATTCGCGTGGACCGGCCGGAGTCGCTGGGTCGCGCGTGGGATGAGGCACTGAGCG
CAACCCGGCCCGTCGTCTTCGAGGCCTACGTGGACCCGGACGTCCCGCTGCTACCGCCCCACATCACCTTCGAGCAGGCG
AAGCACTATGCCCAGGCGCTGGCGAAGGGCGACGTGGACACGGGCGGCATCCTGAAGCAGTCGCTCAAAGGCATGGTGGA
GACACTGCTGCCCCGGGGCGGTGGCAAGTCCTGA

Upstream 100 bases:

>100_bases
GTCGCTGCCCCGGCCGCTGGACGGGCGGATGGGAGCTCGGCCGCAGGCAGCGGTGCCCGGGTGCCTAGCTTGATGGCGTC
CCTGAACCCGAGGAGCCGTC

Downstream 100 bases:

>100_bases
ACCATTCCTTGTCCATCCTTCAGCCCCATCATCCGTTGCGTTAGATTTTCAGCATGGTGGTGAAGATTGCCTCGCGGCTG
GAGCCTGTGCGTCACCCCGA

Product: thiamine pyrophosphate protein

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MSATVSDYLLHRLSQWGVRRIYGYPGDGINGILGALGRTLEFRFVQTRHEEMSAFMACAHAKFTGEPGVCLATSGPGAIH
LLNGLYDAKLDHQPVVAIVGQQTRSALGGHFQQEVDLMSLFKDVASEYLTMVTSPSAIRHAVDRAMRVAIYERTVTCVII
PNDVQEEAYHKPPHAHGTVHSSVGYSVPRVLPRERDLRRAADVLNAGKKVAMLVGAGAQAAADEVMHVAELLGAGVAKAL
LGKAVLPDDVPYVTGSIGLLGTKASWDLMSDCDTLLMVGTSFPYSEFLPKEGQARGVQIDLDGRMLAIRYPMEVALVGDS
QETLKALIPMLEHKKDRSWRDSVVKNVRQWEKTAEALAMDRANPVNPRRVFFELSSRLPDGVILTADSGSGTSWYAQHLK
MRKGMMASLSGNLASMGCGVPYAIGAKFAFPHRPVIALVGDGAMQMNGNAELITVAKYWKEWKDPRFIVMVLNNRDLNMV
TWEQRVLAGDPKLPASQDLPDFPYAAYAESLGFLGIRVDRPESLGRAWDEALSATRPVVFEAYVDPDVPLLPPHITFEQA
KHYAQALAKGDVDTGGILKQSLKGMVETLLPRGGGKS

Sequences:

>Translated_597_residues
MSATVSDYLLHRLSQWGVRRIYGYPGDGINGILGALGRTLEFRFVQTRHEEMSAFMACAHAKFTGEPGVCLATSGPGAIH
LLNGLYDAKLDHQPVVAIVGQQTRSALGGHFQQEVDLMSLFKDVASEYLTMVTSPSAIRHAVDRAMRVAIYERTVTCVII
PNDVQEEAYHKPPHAHGTVHSSVGYSVPRVLPRERDLRRAADVLNAGKKVAMLVGAGAQAAADEVMHVAELLGAGVAKAL
LGKAVLPDDVPYVTGSIGLLGTKASWDLMSDCDTLLMVGTSFPYSEFLPKEGQARGVQIDLDGRMLAIRYPMEVALVGDS
QETLKALIPMLEHKKDRSWRDSVVKNVRQWEKTAEALAMDRANPVNPRRVFFELSSRLPDGVILTADSGSGTSWYAQHLK
MRKGMMASLSGNLASMGCGVPYAIGAKFAFPHRPVIALVGDGAMQMNGNAELITVAKYWKEWKDPRFIVMVLNNRDLNMV
TWEQRVLAGDPKLPASQDLPDFPYAAYAESLGFLGIRVDRPESLGRAWDEALSATRPVVFEAYVDPDVPLLPPHITFEQA
KHYAQALAKGDVDTGGILKQSLKGMVETLLPRGGGKS
>Mature_596_residues
SATVSDYLLHRLSQWGVRRIYGYPGDGINGILGALGRTLEFRFVQTRHEEMSAFMACAHAKFTGEPGVCLATSGPGAIHL
LNGLYDAKLDHQPVVAIVGQQTRSALGGHFQQEVDLMSLFKDVASEYLTMVTSPSAIRHAVDRAMRVAIYERTVTCVIIP
NDVQEEAYHKPPHAHGTVHSSVGYSVPRVLPRERDLRRAADVLNAGKKVAMLVGAGAQAAADEVMHVAELLGAGVAKALL
GKAVLPDDVPYVTGSIGLLGTKASWDLMSDCDTLLMVGTSFPYSEFLPKEGQARGVQIDLDGRMLAIRYPMEVALVGDSQ
ETLKALIPMLEHKKDRSWRDSVVKNVRQWEKTAEALAMDRANPVNPRRVFFELSSRLPDGVILTADSGSGTSWYAQHLKM
RKGMMASLSGNLASMGCGVPYAIGAKFAFPHRPVIALVGDGAMQMNGNAELITVAKYWKEWKDPRFIVMVLNNRDLNMVT
WEQRVLAGDPKLPASQDLPDFPYAAYAESLGFLGIRVDRPESLGRAWDEALSATRPVVFEAYVDPDVPLLPPHITFEQAK
HYAQALAKGDVDTGGILKQSLKGMVETLLPRGGGKS

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=23.4042553191489, Blast_Score=130, Evalue=4e-30,
Organism=Escherichia coli, GI1787096, Length=566, Percent_Identity=34.982332155477, Blast_Score=296, Evalue=3e-81,
Organism=Escherichia coli, GI87081685, Length=545, Percent_Identity=28.256880733945, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1790104, Length=492, Percent_Identity=27.2357723577236, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1786717, Length=562, Percent_Identity=24.3772241992883, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788716, Length=578, Percent_Identity=23.356401384083, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17531299, Length=587, Percent_Identity=24.3611584327087, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17531301, Length=587, Percent_Identity=24.3611584327087, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=568, Percent_Identity=27.8169014084507, Blast_Score=177, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6320816, Length=566, Percent_Identity=21.3780918727915, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922626, Length=485, Percent_Identity=23.9175257731959, Blast_Score=109, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 65070; Mature: 64938

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATVSDYLLHRLSQWGVRRIYGYPGDGINGILGALGRTLEFRFVQTRHEEMSAFMACAH
CCCCHHHHHHHHHHHHCHHEECCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHH
AKFTGEPGVCLATSGPGAIHLLNGLYDAKLDHQPVVAIVGQQTRSALGGHFQQEVDLMSL
CCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHCCHHHHHHHHHHH
FKDVASEYLTMVTSPSAIRHAVDRAMRVAIYERTVTCVIIPNDVQEEAYHKPPHAHGTVH
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECEEEEEEECCCHHHHHHCCCCCCCCCEE
SSVGYSVPRVLPRERDLRRAADVLNAGKKVAMLVGAGAQAAADEVMHVAELLGAGVAKAL
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
LGKAVLPDDVPYVTGSIGLLGTKASWDLMSDCDTLLMVGTSFPYSEFLPKEGQARGVQID
HCCCCCCCCCCEEECCCEEEECCCCCCHHHCCCEEEEEECCCCHHHHCCCCCCCCCEEEE
LDGRMLAIRYPMEVALVGDSQETLKALIPMLEHKKDRSWRDSVVKNVRQWEKTAEALAMD
ECCEEEEEECCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHC
RANPVNPRRVFFELSSRLPDGVILTADSGSGTSWYAQHLKMRKGMMASLSGNLASMGCGV
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHCCCHHHHCCCC
PYAIGAKFAFPHRPVIALVGDGAMQMNGNAELITVAKYWKEWKDPRFIVMVLNNRDLNMV
CHHHCCEECCCCCCEEEEECCCCEEECCCCCEEEHHHHHHHCCCCEEEEEEECCCCCCEE
TWEQRVLAGDPKLPASQDLPDFPYAAYAESLGFLGIRVDRPESLGRAWDEALSATRPVVF
EHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEE
EAYVDPDVPLLPPHITFEQAKHYAQALAKGDVDTGGILKQSLKGMVETLLPRGGGKS
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SATVSDYLLHRLSQWGVRRIYGYPGDGINGILGALGRTLEFRFVQTRHEEMSAFMACAH
CCCHHHHHHHHHHHHCHHEECCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHH
AKFTGEPGVCLATSGPGAIHLLNGLYDAKLDHQPVVAIVGQQTRSALGGHFQQEVDLMSL
CCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHCCHHHHHHHHHHH
FKDVASEYLTMVTSPSAIRHAVDRAMRVAIYERTVTCVIIPNDVQEEAYHKPPHAHGTVH
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECEEEEEEECCCHHHHHHCCCCCCCCCEE
SSVGYSVPRVLPRERDLRRAADVLNAGKKVAMLVGAGAQAAADEVMHVAELLGAGVAKAL
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
LGKAVLPDDVPYVTGSIGLLGTKASWDLMSDCDTLLMVGTSFPYSEFLPKEGQARGVQID
HCCCCCCCCCCEEECCCEEEECCCCCCHHHCCCEEEEEECCCCHHHHCCCCCCCCCEEEE
LDGRMLAIRYPMEVALVGDSQETLKALIPMLEHKKDRSWRDSVVKNVRQWEKTAEALAMD
ECCEEEEEECCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHC
RANPVNPRRVFFELSSRLPDGVILTADSGSGTSWYAQHLKMRKGMMASLSGNLASMGCGV
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHCCCHHHHCCCC
PYAIGAKFAFPHRPVIALVGDGAMQMNGNAELITVAKYWKEWKDPRFIVMVLNNRDLNMV
CHHHCCEECCCCCCEEEEECCCCEEECCCCCEEEHHHHHHHCCCCEEEEEEECCCCCCEE
TWEQRVLAGDPKLPASQDLPDFPYAAYAESLGFLGIRVDRPESLGRAWDEALSATRPVVF
EHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEE
EAYVDPDVPLLPPHITFEQAKHYAQALAKGDVDTGGILKQSLKGMVETLLPRGGGKS
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0022 {lysolecithin}} 0.0017 {lecithin}} 0.03 {sodium} 2 {ferricyanide}} 0.0012 {thiamine} 11 {pyruvate}} 10 {pyruvate}} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]