Click here to switch to the map view.

The map label for this gene is bioB

Identifier: 108760442

GI number: 108760442

Start: 1469203

End: 1470243

Strand: Direct

Name: bioB

Synonym: MXAN_1257

Alternate gene names: 108760442

Gene position: 1469203-1470243 (Clockwise)

Preceding gene: 108757462

Following gene: 108759040

Centisome position: 16.07

GC content: 68.78

Gene sequence:

>1041_bases
ATGCCCGATACCGCCACTGTCTCCAGCGAGAGCGAGTTCTCTGGTCACGCCCACGTCGCCGCGCCGCCACCGGCGGGCGT
GGACGTGCGCCATGACTGGTCGCTGTCGGAGGTGAGGGCCCTCTACGAACTCCCGCTGCTGGACCTGGTGCACAAGGCCC
AGACGGTCCACCGGGCCGTGTTCGTGGACAACAAGGTCCAGCTCTGTTCGCTGCTGTCCATCAAGACGGGCGGCTGCCCG
GAGGACTGCTCGTACTGCCCGCAGGCGGCTCGCTACAAGACGGGCGTCAAGGCGGAGAAGCTGATGGCCGTGCCGGACGT
GCTGGATGCCGCGTCCAAGGCCCGCGCCGCCGGAGCCACCCGCTTCTGCATGGGCGCCGCGTGGCGCGAGGTGAAGGACG
GTCCGCAGTTCGACAGCGTGCTGGAGATGGTGCGCGGCGTGCGCGCGCTGGGCATGGAGGCGTGTGCCACCCTGGGCATG
CTCTCCGAGAGCCAGGCGAAGCGCCTGCGCGAGGCCGGCCTGTCCGCGTACAACCACAACCTGGACACGTCGCCCGAGCA
CTACGGCGACATCATCTCCACCCGCACCTATGAAGACCGCCTGCGCACGCTCAACCGCGTGCGCGACGCCGGCATCTCCG
TGTGCTGCGGCGGCATCATTGGCATGGGCGAGTCGGTGGATGACCGCTGCAACCTGCTGCGCACCCTGGCCAACCAGGAG
CACCACCCGGAGTCGGTGCCCATCAACGCGCTGGTGGCCGTGGAGGGCACGCCGCTGCAGGAGCAGCAGCGCGTGGAGAC
GGTGGACATGGTGCGCACCATCGCCACCGCGCGCATCCTGATGCCCCAGTCCATGGTGCGCCTGTCCGCGGGCCGGCAGC
AGATGAACGAAGAGGCGCAACTGCTGTGCATGATGGCGGGCGCCAACTCACTCTTCTTCGGCGAGAAGCTGCTCACCACC
GGCAACCCCGAGTACACCCAGGACATGGCCCTGCTGGAGAAGGCGGGAATCCGCCCCCTGGAGCCGCGTCAGGAAGGCTG
A

Upstream 100 bases:

>100_bases
AGATGGACCCTGGAGAGTCGTCAACCAACCTGCTCGCGGGCGTTGACAACTTTCCGGGGTCCACGCACGTTCGGGCCCTC
ATCCGTCCGTAGAGGAGCCC

Downstream 100 bases:

>100_bases
GCCCACCGTGAGTGATGAAGCCGTCGCGGCGGCGTGGGCCCGGGAGGACCTGGAGGCCCTGTCCGAGCGCGGCCTGCGCC
GCTACCTGGAGCCCCTGGAC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MPDTATVSSESEFSGHAHVAAPPPAGVDVRHDWSLSEVRALYELPLLDLVHKAQTVHRAVFVDNKVQLCSLLSIKTGGCP
EDCSYCPQAARYKTGVKAEKLMAVPDVLDAASKARAAGATRFCMGAAWREVKDGPQFDSVLEMVRGVRALGMEACATLGM
LSESQAKRLREAGLSAYNHNLDTSPEHYGDIISTRTYEDRLRTLNRVRDAGISVCCGGIIGMGESVDDRCNLLRTLANQE
HHPESVPINALVAVEGTPLQEQQRVETVDMVRTIATARILMPQSMVRLSAGRQQMNEEAQLLCMMAGANSLFFGEKLLTT
GNPEYTQDMALLEKAGIRPLEPRQEG

Sequences:

>Translated_346_residues
MPDTATVSSESEFSGHAHVAAPPPAGVDVRHDWSLSEVRALYELPLLDLVHKAQTVHRAVFVDNKVQLCSLLSIKTGGCP
EDCSYCPQAARYKTGVKAEKLMAVPDVLDAASKARAAGATRFCMGAAWREVKDGPQFDSVLEMVRGVRALGMEACATLGM
LSESQAKRLREAGLSAYNHNLDTSPEHYGDIISTRTYEDRLRTLNRVRDAGISVCCGGIIGMGESVDDRCNLLRTLANQE
HHPESVPINALVAVEGTPLQEQQRVETVDMVRTIATARILMPQSMVRLSAGRQQMNEEAQLLCMMAGANSLFFGEKLLTT
GNPEYTQDMALLEKAGIRPLEPRQEG
>Mature_345_residues
PDTATVSSESEFSGHAHVAAPPPAGVDVRHDWSLSEVRALYELPLLDLVHKAQTVHRAVFVDNKVQLCSLLSIKTGGCPE
DCSYCPQAARYKTGVKAEKLMAVPDVLDAASKARAAGATRFCMGAAWREVKDGPQFDSVLEMVRGVRALGMEACATLGML
SESQAKRLREAGLSAYNHNLDTSPEHYGDIISTRTYEDRLRTLNRVRDAGISVCCGGIIGMGESVDDRCNLLRTLANQEH
HPESVPINALVAVEGTPLQEQQRVETVDMVRTIATARILMPQSMVRLSAGRQQMNEEAQLLCMMAGANSLFFGEKLLTTG
NPEYTQDMALLEKAGIRPLEPRQEG

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=312, Percent_Identity=61.5384615384615, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321725, Length=318, Percent_Identity=49.3710691823899, Blast_Score=347, Evalue=1e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_MYXXD (Q1DCV9)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_629514.1
- ProteinModelPortal:   Q1DCV9
- SMR:   Q1DCV9
- STRING:   Q1DCV9
- GeneID:   4102439
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_1257
- TIGR:   MXAN_1257
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q1DCV9
- BioCyc:   MXAN246197:MXAN_1257-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37709; Mature: 37577

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTATVSSESEFSGHAHVAAPPPAGVDVRHDWSLSEVRALYELPLLDLVHKAQTVHRAV
CCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FVDNKVQLCSLLSIKTGGCPEDCSYCPQAARYKTGVKAEKLMAVPDVLDAASKARAAGAT
HCCCHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHH
RFCMGAAWREVKDGPQFDSVLEMVRGVRALGMEACATLGMLSESQAKRLREAGLSAYNHN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDTSPEHYGDIISTRTYEDRLRTLNRVRDAGISVCCGGIIGMGESVDDRCNLLRTLANQE
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
HHPESVPINALVAVEGTPLQEQQRVETVDMVRTIATARILMPQSMVRLSAGRQQMNEEAQ
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
LLCMMAGANSLFFGEKLLTTGNPEYTQDMALLEKAGIRPLEPRQEG
EEHHHHCCCCHHCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PDTATVSSESEFSGHAHVAAPPPAGVDVRHDWSLSEVRALYELPLLDLVHKAQTVHRAV
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FVDNKVQLCSLLSIKTGGCPEDCSYCPQAARYKTGVKAEKLMAVPDVLDAASKARAAGAT
HCCCHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHH
RFCMGAAWREVKDGPQFDSVLEMVRGVRALGMEACATLGMLSESQAKRLREAGLSAYNHN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDTSPEHYGDIISTRTYEDRLRTLNRVRDAGISVCCGGIIGMGESVDDRCNLLRTLANQE
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
HHPESVPINALVAVEGTPLQEQQRVETVDMVRTIATARILMPQSMVRLSAGRQQMNEEAQ
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
LLCMMAGANSLFFGEKLLTTGNPEYTQDMALLEKAGIRPLEPRQEG
EEHHHHCCCCHHCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA