Click here to switch to the map view.

The map label for this gene is rsmE [H]

Identifier: 108760347

GI number: 108760347

Start: 1320553

End: 1321287

Strand: Reverse

Name: rsmE [H]

Synonym: MXAN_1133

Alternate gene names: 108760347

Gene position: 1321287-1320553 (Counterclockwise)

Preceding gene: 108762573

Following gene: 108757980

Centisome position: 14.46

GC content: 71.56

Gene sequence:

>735_bases
TTGGTCCGCCTCTTCGCCCCGTTGCCCGAGCCCGCTCCCGCCGAAGTGGAGCTGACCGGCGAGCGTCGTCACTACCTCCT
GCATGTCCTGCGGCTGGAGGAAGGCGACGCGCTGGAGGTCTTCGACGGGAAGGGCCGGGCCTTCGAGGCCCGCGTGGCCC
ACGTCACGCCAGATGTCGTGCGCGTGACGTTGGGCACCGCGCGCGTGGCCCCCCCGCGCCGCGAGGTCAGCATCCTTCAA
GGCCTGCCCAAGGGCGACAAGCTGGAGCTGGTGCTCCAGAAAGGCACCGAGCTGGGCGCCACCGCCTTCCACCCCGTGGC
CACGGCGCGAAGTGTCGTGAAGCTCGAGCCCAAGCGCGCGGAGGAGCGCACCTCCCGGTGGACGAAGATTGTCGAGGAGG
CCGCGCGGCAATGCCGCAGGGACGACGTGCCACGCGTGGCCACGCCTGCCCCACTGCTGGATGCGGCTCGCTCGCTGACC
GCGGGCACCGTGCTGCTCGTGCTCGACGAGGAAGAGTCGGCCGTGCCCTTGGGCGAAGCGTTCCGGGCCGCGGGCGCGGG
CACCGCCGTGGCACTGGTGATTGGCCCCGAAGGGGGGCTTGCGCGTGAAGAAGTGGATGCCTTGATGGCCCTGGGCGCGC
GGCCAGTCACCCTGGGCAAGCGCATCCTCCGTACCGAGACGGCGGCGCTCGCGGCGCTGGCGGTGATGATGCACCTGGAC
GGCGAACTCGGGTAG

Upstream 100 bases:

>100_bases
GCCAGGACGCGTACGGAGCAACTGTCTGCCAACCTGACACCTTGAAGTCGGTGGCCCCCAGGGTCAGAGTCCAGCCCCCC
CGCGCTCTGGAGAAACCGCA

Downstream 100 bases:

>100_bases
CAGGGCGGCAGCCGACCGCACGGAGTGGGCGGTTCACACTGTCCGAAGATGATTCCTACGTTTCTGCCGTAGGGGACCTC
AGGGGGACCCTCAACACAGG

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MVRLFAPLPEPAPAEVELTGERRHYLLHVLRLEEGDALEVFDGKGRAFEARVAHVTPDVVRVTLGTARVAPPRREVSILQ
GLPKGDKLELVLQKGTELGATAFHPVATARSVVKLEPKRAEERTSRWTKIVEEAARQCRRDDVPRVATPAPLLDAARSLT
AGTVLLVLDEEESAVPLGEAFRAAGAGTAVALVIGPEGGLAREEVDALMALGARPVTLGKRILRTETAALAALAVMMHLD
GELG

Sequences:

>Translated_244_residues
MVRLFAPLPEPAPAEVELTGERRHYLLHVLRLEEGDALEVFDGKGRAFEARVAHVTPDVVRVTLGTARVAPPRREVSILQ
GLPKGDKLELVLQKGTELGATAFHPVATARSVVKLEPKRAEERTSRWTKIVEEAARQCRRDDVPRVATPAPLLDAARSLT
AGTVLLVLDEEESAVPLGEAFRAAGAGTAVALVIGPEGGLAREEVDALMALGARPVTLGKRILRTETAALAALAVMMHLD
GELG
>Mature_244_residues
MVRLFAPLPEPAPAEVELTGERRHYLLHVLRLEEGDALEVFDGKGRAFEARVAHVTPDVVRVTLGTARVAPPRREVSILQ
GLPKGDKLELVLQKGTELGATAFHPVATARSVVKLEPKRAEERTSRWTKIVEEAARQCRRDDVPRVATPAPLLDAARSLT
AGTVLLVLDEEESAVPLGEAFRAAGAGTAVALVIGPEGGLAREEVDALMALGARPVTLGKRILRTETAALAALAVMMHLD
GELG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=251, Percent_Identity=29.4820717131474, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26097; Mature: 26097

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRLFAPLPEPAPAEVELTGERRHYLLHVLRLEEGDALEVFDGKGRAFEARVAHVTPDVV
CEEEECCCCCCCCCEEEECCCHHEEEEEEEEECCCCEEEEECCCCCEEEEEEECCCCCCE
RVTLGTARVAPPRREVSILQGLPKGDKLELVLQKGTELGATAFHPVATARSVVKLEPKRA
EEEECCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHEEECCHHH
EERTSRWTKIVEEAARQCRRDDVPRVATPAPLLDAARSLTAGTVLLVLDEEESAVPLGEA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHH
FRAAGAGTAVALVIGPEGGLAREEVDALMALGARPVTLGKRILRTETAALAALAVMMHLD
HHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GELG
CCCC
>Mature Secondary Structure
MVRLFAPLPEPAPAEVELTGERRHYLLHVLRLEEGDALEVFDGKGRAFEARVAHVTPDVV
CEEEECCCCCCCCCEEEECCCHHEEEEEEEEECCCCEEEEECCCCCEEEEEEECCCCCCE
RVTLGTARVAPPRREVSILQGLPKGDKLELVLQKGTELGATAFHPVATARSVVKLEPKRA
EEEECCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHEEECCHHH
EERTSRWTKIVEEAARQCRRDDVPRVATPAPLLDAARSLTAGTVLLVLDEEESAVPLGEA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHH
FRAAGAGTAVALVIGPEGGLAREEVDALMALGARPVTLGKRILRTETAALAALAVMMHLD
HHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GELG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]