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The map label for this gene is gltX

Identifier: 108760344

GI number: 108760344

Start: 3121538

End: 3122977

Strand: Reverse

Name: gltX

Synonym: MXAN_2675

Alternate gene names: 108760344

Gene position: 3122977-3121538 (Counterclockwise)

Preceding gene: 108762484

Following gene: 108760997

Centisome position: 34.17

GC content: 67.78

Gene sequence:

>1440_bases
ATGCCTCCTGCCCTTCGCGTCCGCTTCGCTCCCTCGCCTACCGGCTACCTTCACATTGGCGGTGCCCGTACGGCGCTGAT
GAACTTTCTCCAGGCGCGGCGCCAGGGCGGCACCTTCGTCCTTCGCATGGAGGACACGGACCAGGGCCGCTCCACCCCGG
AGTCGGTGCAGGCCATCCTGGACGGGCTGAACTGGCTGGGCATCGACTGGGATGAGGGCCCCGGTAAGGAAGGCCCCTAC
GCCCCCTACTTCCAGATGCAGCGGCTGGACACCTATCGCAAGCACGCCGACCAGCTCATCGCCGAGGGCAAGGCCTACCG
GTGCTACTGCACGAAGGAGGACCTCGACGCGCAGCGGCAGGTGGCGGAGAAGGCGGGCGGCGCGTTCAAGTACCCGGGCA
CCTGCCGCGAGCGGACCGAGCCCCCCGCCGGCCGCAACGCCGCGGACGCCGTCATCCGCTTCAAGATGCCCGCGGGTGAC
GGTTCCGTGTCCTTCACCGACAAGGCGCTGGGCACCATCACCAAGACGCACAGCGACCTGGATGACTGGGTCATGATGCG
GGCGGACGGCATCCCCGTGTACAACTTCGGGTGTGTCATTGATGACCACCTGATGGACATCACCCTGGTCGCGCGCGGCC
AGGAGCACGTCAACTCCACCTTCCCGCAGCTCATGCTGTACCAGGCGCTGGGGTGGACGCCTCCCGACTTCGCCCACCTG
CCGCTCATCCTGGGCCCGGACCGCGAGAAGCTGTCCAAGCGCAAGCACCCGGAAGCGGACGTGATGGTGCACAAGCGCAA
CGGCGTCATGCCGGAGGCGCTGCTCAACTTCGTCATCCGCCTGGGCTGGAGCCACGGCAACGACGAGGTCATCAGCCGCG
AACAGATGCTCGAGTGGTTCGACTTCAGCGACGTGGGCACCACCTCCGGCGTGTGGAACCCGGAGAAGCTGCTGTGGCTC
AACCAGCAGTGGATGAAGCAGTTGCCGGTGGAGACCGTGGTGGAGCGGCTGCTCCCCTTCCTGGAGGCGAAGGGCATCCA
GGCGAAGGGCGACCCGCGGCTGGAGACGCTGGTGCGCACGCTGCGCGAGCGCTCCAACACCCTGGAAGACATCGCCACCA
CCGCGGCCAACGTCTACTTCCGCTCCGGCATCACCCTGGACGAGAAGGCCGCCACCAAGCACCTCTCCGGAGAGTCCCTC
AACCTGCTGCGCAAGGTGCGCGAGACGCTCACCGCGCTGCCGGAGTGGTCGGTGGAGGCGCTGGACGGCGTGGTGAAGCA
GGTGAGCGAGGCCTCCAGCGTCGGCATGGGCAAGGTGGCGCAGCCCATCCGCGTGGCACTCACTGGCAACACCACCAGCC
CGGGCATCGGCGAGACGCTGGTGCTGGTAGGCCGCGACGAATCCCTGCTGCGCATCGACGCGGCGCTGACGCGCGGTTGA

Upstream 100 bases:

>100_bases
GACGAATCGACATACGAGTTCTCCCTGGCCATCCAACTTACTCCAGGAATGCCCCTCCGCGTTGACCCTCCATCCTCGCC
CTGCTAATCCGCGCGCCCGT

Downstream 100 bases:

>100_bases
GCCATCAGGCGCAGGTAGCGGTGGACGTCAGCAGCGCAGGCCCCTATCTTTCGGACCCATGATGCGTGCCCTTGACAGCC
TGAAACGTGGTTTCTATAAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MPPALRVRFAPSPTGYLHIGGARTALMNFLQARRQGGTFVLRMEDTDQGRSTPESVQAILDGLNWLGIDWDEGPGKEGPY
APYFQMQRLDTYRKHADQLIAEGKAYRCYCTKEDLDAQRQVAEKAGGAFKYPGTCRERTEPPAGRNAADAVIRFKMPAGD
GSVSFTDKALGTITKTHSDLDDWVMMRADGIPVYNFGCVIDDHLMDITLVARGQEHVNSTFPQLMLYQALGWTPPDFAHL
PLILGPDREKLSKRKHPEADVMVHKRNGVMPEALLNFVIRLGWSHGNDEVISREQMLEWFDFSDVGTTSGVWNPEKLLWL
NQQWMKQLPVETVVERLLPFLEAKGIQAKGDPRLETLVRTLRERSNTLEDIATTAANVYFRSGITLDEKAATKHLSGESL
NLLRKVRETLTALPEWSVEALDGVVKQVSEASSVGMGKVAQPIRVALTGNTTSPGIGETLVLVGRDESLLRIDAALTRG

Sequences:

>Translated_479_residues
MPPALRVRFAPSPTGYLHIGGARTALMNFLQARRQGGTFVLRMEDTDQGRSTPESVQAILDGLNWLGIDWDEGPGKEGPY
APYFQMQRLDTYRKHADQLIAEGKAYRCYCTKEDLDAQRQVAEKAGGAFKYPGTCRERTEPPAGRNAADAVIRFKMPAGD
GSVSFTDKALGTITKTHSDLDDWVMMRADGIPVYNFGCVIDDHLMDITLVARGQEHVNSTFPQLMLYQALGWTPPDFAHL
PLILGPDREKLSKRKHPEADVMVHKRNGVMPEALLNFVIRLGWSHGNDEVISREQMLEWFDFSDVGTTSGVWNPEKLLWL
NQQWMKQLPVETVVERLLPFLEAKGIQAKGDPRLETLVRTLRERSNTLEDIATTAANVYFRSGITLDEKAATKHLSGESL
NLLRKVRETLTALPEWSVEALDGVVKQVSEASSVGMGKVAQPIRVALTGNTTSPGIGETLVLVGRDESLLRIDAALTRG
>Mature_478_residues
PPALRVRFAPSPTGYLHIGGARTALMNFLQARRQGGTFVLRMEDTDQGRSTPESVQAILDGLNWLGIDWDEGPGKEGPYA
PYFQMQRLDTYRKHADQLIAEGKAYRCYCTKEDLDAQRQVAEKAGGAFKYPGTCRERTEPPAGRNAADAVIRFKMPAGDG
SVSFTDKALGTITKTHSDLDDWVMMRADGIPVYNFGCVIDDHLMDITLVARGQEHVNSTFPQLMLYQALGWTPPDFAHLP
LILGPDREKLSKRKHPEADVMVHKRNGVMPEALLNFVIRLGWSHGNDEVISREQMLEWFDFSDVGTTSGVWNPEKLLWLN
QQWMKQLPVETVVERLLPFLEAKGIQAKGDPRLETLVRTLRERSNTLEDIATTAANVYFRSGITLDEKAATKHLSGESLN
LLRKVRETLTALPEWSVEALDGVVKQVSEASSVGMGKVAQPIRVALTGNTTSPGIGETLVLVGRDESLLRIDAALTRG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=500, Percent_Identity=32.2, Blast_Score=237, Evalue=2e-62,
Organism=Escherichia coli, GI1788743, Length=472, Percent_Identity=42.7966101694915, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI226510925, Length=295, Percent_Identity=34.9152542372881, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1786895, Length=212, Percent_Identity=27.8301886792453, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=476, Percent_Identity=32.9831932773109, Blast_Score=253, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6324540, Length=500, Percent_Identity=31.6, Blast_Score=220, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6324742, Length=137, Percent_Identity=32.8467153284672, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24665321, Length=494, Percent_Identity=33.8056680161943, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_MYXXD (Q1D8Y1)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_630892.1
- ProteinModelPortal:   Q1D8Y1
- SMR:   Q1D8Y1
- STRING:   Q1D8Y1
- GeneID:   4103882
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_2675
- TIGR:   MXAN_2675
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   NEIFAKR
- PhylomeDB:   Q1D8Y1
- BioCyc:   MXAN246197:MXAN_2675-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 53209; Mature: 53078

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPALRVRFAPSPTGYLHIGGARTALMNFLQARRQGGTFVLRMEDTDQGRSTPESVQAIL
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHH
DGLNWLGIDWDEGPGKEGPYAPYFQMQRLDTYRKHADQLIAEGKAYRCYCTKEDLDAQRQ
CCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHCCCHHHH
VAEKAGGAFKYPGTCRERTEPPAGRNAADAVIRFKMPAGDGSVSFTDKALGTITKTHSDL
HHHHCCCCCCCCCCCHHCCCCCCCCCCCCEEEEEEECCCCCCCEECHHHHHHHHHCCCCH
DDWVMMRADGIPVYNFGCVIDDHLMDITLVARGQEHVNSTFPQLMLYQALGWTPPDFAHL
HHHHEEEECCCCEEEECCEEECCHHEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCC
PLILGPDREKLSKRKHPEADVMVHKRNGVMPEALLNFVIRLGWSHGNDEVISREQMLEWF
CEEECCCHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DFSDVGTTSGVWNPEKLLWLNQQWMKQLPVETVVERLLPFLEAKGIQAKGDPRLETLVRT
CCCCCCCCCCCCCCHHEEEECHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LRERSNTLEDIATTAANVYFRSGITLDEKAATKHLSGESLNLLRKVRETLTALPEWSVEA
HHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHH
LDGVVKQVSEASSVGMGKVAQPIRVALTGNTTSPGIGETLVLVGRDESLLRIDAALTRG
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEHHHHCCC
>Mature Secondary Structure 
PPALRVRFAPSPTGYLHIGGARTALMNFLQARRQGGTFVLRMEDTDQGRSTPESVQAIL
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHH
DGLNWLGIDWDEGPGKEGPYAPYFQMQRLDTYRKHADQLIAEGKAYRCYCTKEDLDAQRQ
CCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHCCCHHHH
VAEKAGGAFKYPGTCRERTEPPAGRNAADAVIRFKMPAGDGSVSFTDKALGTITKTHSDL
HHHHCCCCCCCCCCCHHCCCCCCCCCCCCEEEEEEECCCCCCCEECHHHHHHHHHCCCCH
DDWVMMRADGIPVYNFGCVIDDHLMDITLVARGQEHVNSTFPQLMLYQALGWTPPDFAHL
HHHHEEEECCCCEEEECCEEECCHHEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCC
PLILGPDREKLSKRKHPEADVMVHKRNGVMPEALLNFVIRLGWSHGNDEVISREQMLEWF
CEEECCCHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DFSDVGTTSGVWNPEKLLWLNQQWMKQLPVETVVERLLPFLEAKGIQAKGDPRLETLVRT
CCCCCCCCCCCCCCHHEEEECHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LRERSNTLEDIATTAANVYFRSGITLDEKAATKHLSGESLNLLRKVRETLTALPEWSVEA
HHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHH
LDGVVKQVSEASSVGMGKVAQPIRVALTGNTTSPGIGETLVLVGRDESLLRIDAALTRG
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA