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The map label for this gene is alsS [H]

Identifier: 108760342

GI number: 108760342

Start: 3311193

End: 3312830

Strand: Direct

Name: alsS [H]

Synonym: MXAN_2843

Alternate gene names: 108760342

Gene position: 3311193-3312830 (Clockwise)

Preceding gene: 108757171

Following gene: 108761886

Centisome position: 36.23

GC content: 69.6

Gene sequence:

>1638_bases
ATGAAGGCGGCAGACCTGTTCGTCAAAGCGTTGGAAGCGGAGGGCGTGCGCAGCGTCTTCGGGCTTCCGGGAGAGGAGAA
CCTGGACGTCATCGAGGCCATGCGGGCCGCGGGCATGCGGCTGGTGCTCACCCGTCATGAGCAGGCCGCGGGCTTCATGG
CGGCCACGTATGGGCGGCTCACCGGCCGCGCGGGCGTCGTTCTGTCGACGCTGGGGCCCGGGGCGACCAACCTCGTCACG
GCCGCCGCCTACGCACAGCTTGGCGCCATGCCCATGGTGATGGTCACCGGGCAGAAGCCCATCCGGGCCGGCAAGCAGGG
CCACTTCCAGATTGTCGACGTGGTGGGGATGATGCGCCCCCTGACGAAGCTGACGCGCACGCTCGTGTCGGCGGAGCATG
TCCCTTCGGCCGTGCGTGAGGCGTTCCGCCGCGCCGAGGAGGAGCGCCCCGGCGCGACGCACCTGGAGCTCCCGGAGGAC
GTGGCCCGGGAGTCGACCGACGCCGTGCTGCTGGCGCCCAGCGCCCATCGCCGGCCCGTGGCGGATGAGGCGTCCATCGC
GCTGGCCGTGGAGGCGATTGCTTCCGCTCGCCGGCCCTTGCTCATGATTGGCGCGGGCGCCAACCGCAAGCTCACGTCGG
AGATGCTGCGCGTCTTCGTGGACAAGACGGGCATTCCCTTCTTCAGCACGCAGATGGGCAAGGGCGTCGTGGACGAGTCG
CATCCCCTGTGGCTGGGCACGGCGGCGCTGTCGGAAGGGGACTTCGTCCACCGCGCCATCGAGGCCTCGGACTGCATCAT
CAACGTGGGCCACGACGTCATCGAGAAGCCGCCGTTCTTCATGCGCGACAGCCGCCGCACGGTGGTGCATCTCAACTTCT
CTTCGGCGGCGGTGGACCCCGTGTACTTCCCGCAACTGGAGGTGACGGGCGACATCGCGAACGCGGTGTGGCGCATCGGT
GAAGGGCTGGGGCCTCGCGCGCACTGGGACTTCGTGCCCTTCGAGAAGTTCCGCGCAGGACTCGAGGCGCAGCTCGCGCA
CGGAGGGGATGACGACCGCTTCCCCATCTACCCCGCGCGGCTCGTCGCCGAGCTACGGCGCGCGCTGCCGGACGACGGCA
TCGTGTGCCTGGACAACGGCATGTACAAGCTGTGGTTCGCTCGCTACTACCGCACGCGCCGGCCCAACACGCTGCTGCTC
GACAACGCGCTGGCGACGATGGGGGCAGGGCTGCCTTCGGCCATCGCGGCGAAGCTGGTGCATCCCCGCCGGAAGGTGGT
GGCGGTCTGCGGAGACGGCGGGTTCATGATGAACTCGCAGGAGCTGGAGACGGCCGTTCGGCTGGGCCTGGACCTGACGG
TGGTCGTCGTGCGCGACGACGGCTACGGGATGATTCGCTGGAAGCAGGGGGAGATGGGCCTGCCGGACTTCGGCATGGCG
CTGGGGAACCCGGACTTCGTTCGCTACGCGGAGGCATATGGGGCCCATGGCCACCGGCCGACCAGCGCGACGGAGTTCGG
CGCGACGCTGGCTCGCTGCCTGGCGTCGAGCGGCGTCCATGTCATCGACGTGCCCATCGACTACTCGGACAACGCACGGG
CGCTCGGCGCGGGCGCGGAGGAACTCGCCGCGCTCTGA

Upstream 100 bases:

>100_bases
CATAGGCATTGCCTATCGAAGCCATGGGAACAAACGAATGTACCAATCATGGCCCGCGCGTAAGTGTTCTCCCATCGCGA
GGTCGTGAAGGAGGCACCCG

Downstream 100 bases:

>100_bases
GGGGGAACGGCGGTTCATGAACACTGGAGGAACACCATGCTGGCTGAACGTTATCCGTACTACCTGGCCAACCGGCCCCG
GCAGCCCAACGCGGAGCTGG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MKAADLFVKALEAEGVRSVFGLPGEENLDVIEAMRAAGMRLVLTRHEQAAGFMAATYGRLTGRAGVVLSTLGPGATNLVT
AAAYAQLGAMPMVMVTGQKPIRAGKQGHFQIVDVVGMMRPLTKLTRTLVSAEHVPSAVREAFRRAEEERPGATHLELPED
VARESTDAVLLAPSAHRRPVADEASIALAVEAIASARRPLLMIGAGANRKLTSEMLRVFVDKTGIPFFSTQMGKGVVDES
HPLWLGTAALSEGDFVHRAIEASDCIINVGHDVIEKPPFFMRDSRRTVVHLNFSSAAVDPVYFPQLEVTGDIANAVWRIG
EGLGPRAHWDFVPFEKFRAGLEAQLAHGGDDDRFPIYPARLVAELRRALPDDGIVCLDNGMYKLWFARYYRTRRPNTLLL
DNALATMGAGLPSAIAAKLVHPRRKVVAVCGDGGFMMNSQELETAVRLGLDLTVVVVRDDGYGMIRWKQGEMGLPDFGMA
LGNPDFVRYAEAYGAHGHRPTSATEFGATLARCLASSGVHVIDVPIDYSDNARALGAGAEELAAL

Sequences:

>Translated_545_residues
MKAADLFVKALEAEGVRSVFGLPGEENLDVIEAMRAAGMRLVLTRHEQAAGFMAATYGRLTGRAGVVLSTLGPGATNLVT
AAAYAQLGAMPMVMVTGQKPIRAGKQGHFQIVDVVGMMRPLTKLTRTLVSAEHVPSAVREAFRRAEEERPGATHLELPED
VARESTDAVLLAPSAHRRPVADEASIALAVEAIASARRPLLMIGAGANRKLTSEMLRVFVDKTGIPFFSTQMGKGVVDES
HPLWLGTAALSEGDFVHRAIEASDCIINVGHDVIEKPPFFMRDSRRTVVHLNFSSAAVDPVYFPQLEVTGDIANAVWRIG
EGLGPRAHWDFVPFEKFRAGLEAQLAHGGDDDRFPIYPARLVAELRRALPDDGIVCLDNGMYKLWFARYYRTRRPNTLLL
DNALATMGAGLPSAIAAKLVHPRRKVVAVCGDGGFMMNSQELETAVRLGLDLTVVVVRDDGYGMIRWKQGEMGLPDFGMA
LGNPDFVRYAEAYGAHGHRPTSATEFGATLARCLASSGVHVIDVPIDYSDNARALGAGAEELAAL
>Mature_545_residues
MKAADLFVKALEAEGVRSVFGLPGEENLDVIEAMRAAGMRLVLTRHEQAAGFMAATYGRLTGRAGVVLSTLGPGATNLVT
AAAYAQLGAMPMVMVTGQKPIRAGKQGHFQIVDVVGMMRPLTKLTRTLVSAEHVPSAVREAFRRAEEERPGATHLELPED
VARESTDAVLLAPSAHRRPVADEASIALAVEAIASARRPLLMIGAGANRKLTSEMLRVFVDKTGIPFFSTQMGKGVVDES
HPLWLGTAALSEGDFVHRAIEASDCIINVGHDVIEKPPFFMRDSRRTVVHLNFSSAAVDPVYFPQLEVTGDIANAVWRIG
EGLGPRAHWDFVPFEKFRAGLEAQLAHGGDDDRFPIYPARLVAELRRALPDDGIVCLDNGMYKLWFARYYRTRRPNTLLL
DNALATMGAGLPSAIAAKLVHPRRKVVAVCGDGGFMMNSQELETAVRLGLDLTVVVVRDDGYGMIRWKQGEMGLPDFGMA
LGNPDFVRYAEAYGAHGHRPTSATEFGATLARCLASSGVHVIDVPIDYSDNARALGAGAEELAAL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=531, Percent_Identity=27.683615819209, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI93004078, Length=548, Percent_Identity=24.4525547445255, Blast_Score=159, Evalue=5e-39,
Organism=Escherichia coli, GI1790104, Length=538, Percent_Identity=29.368029739777, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=28.5968028419183, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1786717, Length=486, Percent_Identity=27.1604938271605, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1787096, Length=536, Percent_Identity=24.2537313432836, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=26.4388489208633, Blast_Score=125, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=481, Percent_Identity=27.027027027027, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=481, Percent_Identity=27.027027027027, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=590, Percent_Identity=24.7457627118644, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=562, Percent_Identity=29.8932384341637, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6320816, Length=488, Percent_Identity=26.2295081967213, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321524, Length=482, Percent_Identity=23.2365145228216, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323073, Length=489, Percent_Identity=22.6993865030675, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=490, Percent_Identity=22.4489795918367, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19922626, Length=499, Percent_Identity=28.2565130260521, Blast_Score=156, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 58828; Mature: 58828

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAADLFVKALEAEGVRSVFGLPGEENLDVIEAMRAAGMRLVLTRHEQAAGFMAATYGRL
CCHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHHH
TGRAGVVLSTLGPGATNLVTAAAYAQLGAMPMVMVTGQKPIRAGKQGHFQIVDVVGMMRP
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHH
LTKLTRTLVSAEHVPSAVREAFRRAEEERPGATHLELPEDVARESTDAVLLAPSAHRRPV
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCC
ADEASIALAVEAIASARRPLLMIGAGANRKLTSEMLRVFVDKTGIPFFSTQMGKGVVDES
CCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEHHCCCCCCCCC
HPLWLGTAALSEGDFVHRAIEASDCIINVGHDVIEKPPFFMRDSRRTVVHLNFSSAAVDP
CCEEEEEHHCCCCCHHHHHHHHCCCEEECCHHHHCCCCCEEECCCCEEEEEECCCCCCCC
VYFPQLEVTGDIANAVWRIGEGLGPRAHWDFVPFEKFRAGLEAQLAHGGDDDRFPIYPAR
EECCCEEECHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCHHH
LVAELRRALPDDGIVCLDNGMYKLWFARYYRTRRPNTLLLDNALATMGAGLPSAIAAKLV
HHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEHHHHHHHCCCCHHHHHHHHH
HPRRKVVAVCGDGGFMMNSQELETAVRLGLDLTVVVVRDDGYGMIRWKQGEMGLPDFGMA
CCCEEEEEEECCCCEEECHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCCHHHH
LGNPDFVRYAEAYGAHGHRPTSATEFGATLARCLASSGVHVIDVPIDYSDNARALGAGAE
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHH
ELAAL
HHHCC
>Mature Secondary Structure
MKAADLFVKALEAEGVRSVFGLPGEENLDVIEAMRAAGMRLVLTRHEQAAGFMAATYGRL
CCHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHHH
TGRAGVVLSTLGPGATNLVTAAAYAQLGAMPMVMVTGQKPIRAGKQGHFQIVDVVGMMRP
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHH
LTKLTRTLVSAEHVPSAVREAFRRAEEERPGATHLELPEDVARESTDAVLLAPSAHRRPV
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCC
ADEASIALAVEAIASARRPLLMIGAGANRKLTSEMLRVFVDKTGIPFFSTQMGKGVVDES
CCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEHHCCCCCCCCC
HPLWLGTAALSEGDFVHRAIEASDCIINVGHDVIEKPPFFMRDSRRTVVHLNFSSAAVDP
CCEEEEEHHCCCCCHHHHHHHHCCCEEECCHHHHCCCCCEEECCCCEEEEEECCCCCCCC
VYFPQLEVTGDIANAVWRIGEGLGPRAHWDFVPFEKFRAGLEAQLAHGGDDDRFPIYPAR
EECCCEEECHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCHHH
LVAELRRALPDDGIVCLDNGMYKLWFARYYRTRRPNTLLLDNALATMGAGLPSAIAAKLV
HHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEHHHHHHHCCCCHHHHHHHHH
HPRRKVVAVCGDGGFMMNSQELETAVRLGLDLTVVVVRDDGYGMIRWKQGEMGLPDFGMA
CCCEEEEEEECCCCEEECHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCCHHHH
LGNPDFVRYAEAYGAHGHRPTSATEFGATLARCLASSGVHVIDVPIDYSDNARALGAGAE
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHH
ELAAL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]