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The map label for this gene is speE1 [H]

Identifier: 108759872

GI number: 108759872

Start: 1375947

End: 1377443

Strand: Direct

Name: speE1 [H]

Synonym: MXAN_1183

Alternate gene names: 108759872

Gene position: 1375947-1377443 (Clockwise)

Preceding gene: 108759593

Following gene: 108762933

Centisome position: 15.05

GC content: 67.13

Gene sequence:

>1497_bases
GTGAACAAGACGCTGCTGTACATCACCGTCATCGTCATCGCGACGTGCGGGCTCATCTATGAGCTCGTCGTCGGGGCGCT
CGCGAGCTACCTGCTCGGCGACTCCATCACCCAGTTCTCCACCGTCATTGGCGGCTACCTGTTCGCCATGGGCATTGGCA
GCTACCTGTCGCGCTACATCGAGCGCGGGGTGGCGCAGCGCTTCGTGGAGGTGGAGCTGGCGGTGGCGCTGCTGGGCGGC
ATCTGCGCGCCGGTGCTGTTCCTCACCTTCACGCTGACGGACCTGTTCCAGGTGGCGCTGTACGGCAGCGTGATTGCCAT
TGGCGCGCTGGTGGGCATGGAGATTCCCCTCCTGCTGCGCATCCTGAAGGACCAGGTCAAGTTCAAGGACCTCATCAGCC
AGGTGCTGTCGCTGGACTACGTGGGGGCGCTCGCGGCCAGCGTGGCCTTCCCGTTGCTGCTGGTGCCGAAGCTGGGGCTG
GTGCGCACGTCCCTGCTGTTCGGCGTGCTGAACGCGGCGGTGGCGCTGTGGAGCACCTGGTTGCTGGCGCCGCTGCTGGG
CAACCCGCTGCGGCTGCGCATCAAGGCGGTGGGCCTGACGGTGCTGCTGCTGGCGGGCTTCGTCATGGGCGACCGGCTCA
CCACCTTCTACGAGGACCAGCTCTACGCGGATGACGTGGTCCACGCGTCCAGTTCGCCGTATCAGCGCATCATCCTCACG
CGCGGCAAGCGGGGCTTCTCGCTGTTCCTCAACGGCAACCTCCAGTTCGCCAGCCTGGACGAGTACCGCTACCACGAGTC
CCTGGTGCACCCGGCCATGGTGCGCGCGGGCAAGGTGGAGAACGTGCTCATCCTGGGCGGTGGAGACGGCCTGGCCGCGC
GGGAAGTGCTGCGCTACCCGGAGGTCCGCTCCGTCACGCTGGTGGACCTGGACCCGGTGATTACGGGGCTGGCGACGAAC
TACGGGGAGCTGGCGAAGCTGAACCGCCACGCGATGACGGACCCGCGGATGCGGGTGGTCAATGGGGACGCGATGCAGTT
CCTCACCGAGGGCGGCGCGCAGTACGACGTGGTGGTGGTGGACTTCCCGGACCCGAACAACTTCGCGCTGGGGAAGCTGT
ACACCACGGGTTTCTACAAGCTGCTCAAGAAGCGCATCGCGCCGGACGGCGTGGCGGTGGTGCAGAGCACCAGCCCGCTG
TTCGCCCGGCGCTCCTTCTGGTGCGTGGAGACGACGCTCAAGGCCGCGGGCTTCTGGACGGAGCCGTACCACGCGCTGGT
GCCGTCGTTCGGTGAGTGGGGCTACGTGTTGATTGCGCACGAGCCGCCCGCCCGGCACCGGCCGCTGCCCGAAGGGCTGC
TCTTCCTGGACGACGCGACGATGGCGTCACTCACCCAGTTCCCTCCGGACATGGGGCCCTTGCCCGTGGAGGTGAACCGG
CTGAACAACCAGGTGCTGGTGCACTACTACGAGGCGGAGTGGCAGCGGTGGAACTGA

Upstream 100 bases:

>100_bases
TTCCTTCATCATCGGTCTGGCCATCATCGTGGCCGCGGCCATCAGCGGCGGATGAGTGCGCGCGCAGCACACCCATGCCT
GTGCGAAGTGAAGTGACGCC

Downstream 100 bases:

>100_bases
CGCGGCGGGAGCTGGTCGCCGCGTTCCTCGGTTCCGCGGTGGCGGCCAGTGCGTGCCGGAAGTCGGGGTCACGGGAGCGT
GTCCCCGGCGCGGTGGTGGA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1 [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MNKTLLYITVIVIATCGLIYELVVGALASYLLGDSITQFSTVIGGYLFAMGIGSYLSRYIERGVAQRFVEVELAVALLGG
ICAPVLFLTFTLTDLFQVALYGSVIAIGALVGMEIPLLLRILKDQVKFKDLISQVLSLDYVGALAASVAFPLLLVPKLGL
VRTSLLFGVLNAAVALWSTWLLAPLLGNPLRLRIKAVGLTVLLLAGFVMGDRLTTFYEDQLYADDVVHASSSPYQRIILT
RGKRGFSLFLNGNLQFASLDEYRYHESLVHPAMVRAGKVENVLILGGGDGLAAREVLRYPEVRSVTLVDLDPVITGLATN
YGELAKLNRHAMTDPRMRVVNGDAMQFLTEGGAQYDVVVVDFPDPNNFALGKLYTTGFYKLLKKRIAPDGVAVVQSTSPL
FARRSFWCVETTLKAAGFWTEPYHALVPSFGEWGYVLIAHEPPARHRPLPEGLLFLDDATMASLTQFPPDMGPLPVEVNR
LNNQVLVHYYEAEWQRWN

Sequences:

>Translated_498_residues
MNKTLLYITVIVIATCGLIYELVVGALASYLLGDSITQFSTVIGGYLFAMGIGSYLSRYIERGVAQRFVEVELAVALLGG
ICAPVLFLTFTLTDLFQVALYGSVIAIGALVGMEIPLLLRILKDQVKFKDLISQVLSLDYVGALAASVAFPLLLVPKLGL
VRTSLLFGVLNAAVALWSTWLLAPLLGNPLRLRIKAVGLTVLLLAGFVMGDRLTTFYEDQLYADDVVHASSSPYQRIILT
RGKRGFSLFLNGNLQFASLDEYRYHESLVHPAMVRAGKVENVLILGGGDGLAAREVLRYPEVRSVTLVDLDPVITGLATN
YGELAKLNRHAMTDPRMRVVNGDAMQFLTEGGAQYDVVVVDFPDPNNFALGKLYTTGFYKLLKKRIAPDGVAVVQSTSPL
FARRSFWCVETTLKAAGFWTEPYHALVPSFGEWGYVLIAHEPPARHRPLPEGLLFLDDATMASLTQFPPDMGPLPVEVNR
LNNQVLVHYYEAEWQRWN
>Mature_498_residues
MNKTLLYITVIVIATCGLIYELVVGALASYLLGDSITQFSTVIGGYLFAMGIGSYLSRYIERGVAQRFVEVELAVALLGG
ICAPVLFLTFTLTDLFQVALYGSVIAIGALVGMEIPLLLRILKDQVKFKDLISQVLSLDYVGALAASVAFPLLLVPKLGL
VRTSLLFGVLNAAVALWSTWLLAPLLGNPLRLRIKAVGLTVLLLAGFVMGDRLTTFYEDQLYADDVVHASSSPYQRIILT
RGKRGFSLFLNGNLQFASLDEYRYHESLVHPAMVRAGKVENVLILGGGDGLAAREVLRYPEVRSVTLVDLDPVITGLATN
YGELAKLNRHAMTDPRMRVVNGDAMQFLTEGGAQYDVVVVDFPDPNNFALGKLYTTGFYKLLKKRIAPDGVAVVQSTSPL
FARRSFWCVETTLKAAGFWTEPYHALVPSFGEWGYVLIAHEPPARHRPLPEGLLFLDDATMASLTQFPPDMGPLPVEVNR
LNNQVLVHYYEAEWQRWN

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=184, Percent_Identity=30.4347826086957, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1786312, Length=213, Percent_Identity=28.6384976525822, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI212645616, Length=180, Percent_Identity=30, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25154106, Length=179, Percent_Identity=30.1675977653631, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323175, Length=235, Percent_Identity=28.936170212766, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6325326, Length=180, Percent_Identity=30, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24645443, Length=179, Percent_Identity=28.4916201117318, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 54837; Mature: 54837

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTLLYITVIVIATCGLIYELVVGALASYLLGDSITQFSTVIGGYLFAMGIGSYLSRYI
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ERGVAQRFVEVELAVALLGGICAPVLFLTFTLTDLFQVALYGSVIAIGALVGMEIPLLLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKDQVKFKDLISQVLSLDYVGALAASVAFPLLLVPKLGLVRTSLLFGVLNAAVALWSTW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LLAPLLGNPLRLRIKAVGLTVLLLAGFVMGDRLTTFYEDQLYADDVVHASSSPYQRIILT
HHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEE
RGKRGFSLFLNGNLQFASLDEYRYHESLVHPAMVRAGKVENVLILGGGDGLAAREVLRYP
CCCCCEEEEEECCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCC
EVRSVTLVDLDPVITGLATNYGELAKLNRHAMTDPRMRVVNGDAMQFLTEGGAQYDVVVV
CCCEEEEEECHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCEEEEEEE
DFPDPNNFALGKLYTTGFYKLLKKRIAPDGVAVVQSTSPLFARRSFWCVETTLKAAGFWT
ECCCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCHHHHHHHHHHCCCCC
EPYHALVPSFGEWGYVLIAHEPPARHRPLPEGLLFLDDATMASLTQFPPDMGPLPVEVNR
CCHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCCCCEEEEC
LNNQVLVHYYEAEWQRWN
CCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MNKTLLYITVIVIATCGLIYELVVGALASYLLGDSITQFSTVIGGYLFAMGIGSYLSRYI
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ERGVAQRFVEVELAVALLGGICAPVLFLTFTLTDLFQVALYGSVIAIGALVGMEIPLLLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKDQVKFKDLISQVLSLDYVGALAASVAFPLLLVPKLGLVRTSLLFGVLNAAVALWSTW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LLAPLLGNPLRLRIKAVGLTVLLLAGFVMGDRLTTFYEDQLYADDVVHASSSPYQRIILT
HHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEE
RGKRGFSLFLNGNLQFASLDEYRYHESLVHPAMVRAGKVENVLILGGGDGLAAREVLRYP
CCCCCEEEEEECCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCC
EVRSVTLVDLDPVITGLATNYGELAKLNRHAMTDPRMRVVNGDAMQFLTEGGAQYDVVVV
CCCEEEEEECHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCEEEEEEE
DFPDPNNFALGKLYTTGFYKLLKKRIAPDGVAVVQSTSPLFARRSFWCVETTLKAAGFWT
ECCCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCHHHHHHHHHHCCCCC
EPYHALVPSFGEWGYVLIAHEPPARHRPLPEGLLFLDDATMASLTQFPPDMGPLPVEVNR
CCHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCCCCEEEEC
LNNQVLVHYYEAEWQRWN
CCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12712204 [H]