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The map label for this gene is yfhA [H]

Identifier: 108759844

GI number: 108759844

Start: 5340147

End: 5341526

Strand: Reverse

Name: yfhA [H]

Synonym: MXAN_4339

Alternate gene names: 108759844

Gene position: 5341526-5340147 (Counterclockwise)

Preceding gene: 108758855

Following gene: 108762897

Centisome position: 58.44

GC content: 70.07

Gene sequence:

>1380_bases
ATGAGTCACACCGAGCGACCCCAGGGGGCGGGCCGTCCAGCCGAAGGGCCTGATTCCGTCCAGCAGACGCGGCCCACAGG
TACAGGAAGGCCCGGGACCTCGGGCGCTGTTCGCCGCTTCCGATTCACCGTCCTCGAAGGTCCGCAGCCTGGGCAGTCGA
AGGACAGCAACGCGGACACGTTTTCCATTGGCTCGCATGGGCTCAACGACTTCGTCATCGAGGAGCCCACCGTCTCGCGC
TTCCACTGCGAGGTGAAGGTCGACCACGACGGCGCCCGCCTGCGGGATTTGGACAGCCGAAATGGCACGGTGCTCGACGG
TGTCCACGTGCGCGACGCGTACCTTCGTGGCGGCAGCATCCTGCGCCTGGGCCGGGTGAGCGTCCGGTTCGACTTCAGCC
CGGAGACGAACCGGCTGCTCATCTCCGACCGCACGACGTTCGGTGAGCTGGTGGGTTCGTCGCCCGTGACGCGCGCCAGC
TTCGCCCTCATGGAGCGAGCCGCCGCGAGCGACGCGACGGTGTTGCTGGAGGGGGAGACGGGCACGGGCAAGAGCCGCGC
CGCCTTCGCCATCCATCAGGCCAGCGCCCGGGCCTCGGGGCCCTTCCTCACCGTGGACTGCGGCGCCATTCCCGGCAACC
TGCTGGAGAGCGAGCTGTTCGGCCATGAGAAGGGCTCCTTCACCGGCGCCATCCAGCGCCGCGTTGGCGCCTTCGAGGAG
GCGGATGGCGGCACCATCTTCCTGGACGAGATTGGCGAGCTGCCCGCGGAGCTGCAGCCGAAGCTGCTGCGCGTCCTGGA
GAACAAGGAGATTCGCCGGCTGGGCGCCAACGGGTACCAGCCCGTCAACGTGCGTGTCATCGCCGCCACGCACCGGGACC
TGCGCGCCGAGGTGAACGCGGCGCGCTTCCGCTCTGACCTGTTCTTCCGCCTCGCGGTGGTGCGCATCATCATCCCCGCG
CTGCGGGAGCGGCCCGAGGACATCCCCCTCATCGCGGAGCGAATCCTCATGGCCTTCGGGGCCGGCTCCGAGCAGCTCGC
GGCGCTGAGCACGCCGGAGTTCATCGCGCAGCTCCAGCACGCCGCGTGGCCGGGGAACGTGCGCGAGCTGCGCAACCACC
TGGAGCGTTGCCTCGTCTTCCAGGACGCCATGCCGCCGGACGCCGAGGAGGTGAGCGCGCAAGCGGTGCTGCGCAGCCTG
GTGGACCCGAAGCAGCCCTACGCGGAGGCCCGCCGCCGCGCGCTGGAGGTCTTCGAGCGCGAGTACCTGGATGCGCTCCT
CAAGCTCCACGGAGGCAAGGTGTCGCAGGCCGCCACCGCCGCGGACATGGACCGCGTGTACCTGTACCGCCTGCTGCGCC
GGCACCGGCTGCGGACGTAG

Upstream 100 bases:

>100_bases
GGAGGACGGGCCGGACTTCGTCGTCGAATGGGGCCTCCGGTAGGTCAAATGGCCACCGGGCCCGCTGGCGTGGCGTCCAC
GGTGATAGCGTCCATTCCTT

Downstream 100 bases:

>100_bases
CCCGCTCACCGGCTGGCGAGTGCATCCGACAGCAGGACCTCGCCCGCCGTGCCTCCCGAAGCCCAGGACTCCAGGCGCAG
CAACTCACCACCCGCCAACC

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSHTERPQGAGRPAEGPDSVQQTRPTGTGRPGTSGAVRRFRFTVLEGPQPGQSKDSNADTFSIGSHGLNDFVIEEPTVSR
FHCEVKVDHDGARLRDLDSRNGTVLDGVHVRDAYLRGGSILRLGRVSVRFDFSPETNRLLISDRTTFGELVGSSPVTRAS
FALMERAAASDATVLLEGETGTGKSRAAFAIHQASARASGPFLTVDCGAIPGNLLESELFGHEKGSFTGAIQRRVGAFEE
ADGGTIFLDEIGELPAELQPKLLRVLENKEIRRLGANGYQPVNVRVIAATHRDLRAEVNAARFRSDLFFRLAVVRIIIPA
LRERPEDIPLIAERILMAFGAGSEQLAALSTPEFIAQLQHAAWPGNVRELRNHLERCLVFQDAMPPDAEEVSAQAVLRSL
VDPKQPYAEARRRALEVFEREYLDALLKLHGGKVSQAATAADMDRVYLYRLLRRHRLRT

Sequences:

>Translated_459_residues
MSHTERPQGAGRPAEGPDSVQQTRPTGTGRPGTSGAVRRFRFTVLEGPQPGQSKDSNADTFSIGSHGLNDFVIEEPTVSR
FHCEVKVDHDGARLRDLDSRNGTVLDGVHVRDAYLRGGSILRLGRVSVRFDFSPETNRLLISDRTTFGELVGSSPVTRAS
FALMERAAASDATVLLEGETGTGKSRAAFAIHQASARASGPFLTVDCGAIPGNLLESELFGHEKGSFTGAIQRRVGAFEE
ADGGTIFLDEIGELPAELQPKLLRVLENKEIRRLGANGYQPVNVRVIAATHRDLRAEVNAARFRSDLFFRLAVVRIIIPA
LRERPEDIPLIAERILMAFGAGSEQLAALSTPEFIAQLQHAAWPGNVRELRNHLERCLVFQDAMPPDAEEVSAQAVLRSL
VDPKQPYAEARRRALEVFEREYLDALLKLHGGKVSQAATAADMDRVYLYRLLRRHRLRT
>Mature_458_residues
SHTERPQGAGRPAEGPDSVQQTRPTGTGRPGTSGAVRRFRFTVLEGPQPGQSKDSNADTFSIGSHGLNDFVIEEPTVSRF
HCEVKVDHDGARLRDLDSRNGTVLDGVHVRDAYLRGGSILRLGRVSVRFDFSPETNRLLISDRTTFGELVGSSPVTRASF
ALMERAAASDATVLLEGETGTGKSRAAFAIHQASARASGPFLTVDCGAIPGNLLESELFGHEKGSFTGAIQRRVGAFEEA
DGGTIFLDEIGELPAELQPKLLRVLENKEIRRLGANGYQPVNVRVIAATHRDLRAEVNAARFRSDLFFRLAVVRIIIPAL
RERPEDIPLIAERILMAFGAGSEQLAALSTPEFIAQLQHAAWPGNVRELRNHLERCLVFQDAMPPDAEEVSAQAVLRSLV
DPKQPYAEARRRALEVFEREYLDALLKLHGGKVSQAATAADMDRVYLYRLLRRHRLRT

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=313, Percent_Identity=45.0479233226837, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1790437, Length=311, Percent_Identity=44.0514469453376, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI87082117, Length=288, Percent_Identity=46.5277777777778, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1788550, Length=320, Percent_Identity=40.3125, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1789087, Length=240, Percent_Identity=48.3333333333333, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1786524, Length=371, Percent_Identity=39.8921832884097, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1790299, Length=226, Percent_Identity=49.1150442477876, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI87082152, Length=349, Percent_Identity=40.4011461318052, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1789233, Length=326, Percent_Identity=36.1963190184049, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI87081872, Length=313, Percent_Identity=38.0191693290735, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1787583, Length=283, Percent_Identity=36.7491166077738, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1789828, Length=229, Percent_Identity=37.117903930131, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87081858, Length=310, Percent_Identity=30, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50439; Mature: 50308

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50006 FHA_DOMAIN ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTERPQGAGRPAEGPDSVQQTRPTGTGRPGTSGAVRRFRFTVLEGPQPGQSKDSNADT
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHEEEEEEECCCCCCCCCCCCCCE
FSIGSHGLNDFVIEEPTVSRFHCEVKVDHDGARLRDLDSRNGTVLDGVHVRDAYLRGGSI
EECCCCCCCCCEECCCCCCEEEEEEEECCCCCHHHCCCCCCCCEECCEEEEHHHHCCCCE
LRLGRVSVRFDFSPETNRLLISDRTTFGELVGSSPVTRASFALMERAAASDATVLLEGET
EEEEEEEEEEECCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCC
GTGKSRAAFAIHQASARASGPFLTVDCGAIPGNLLESELFGHEKGSFTGAIQRRVGAFEE
CCCCCHHEEEEEHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCC
ADGGTIFLDEIGELPAELQPKLLRVLENKEIRRLGANGYQPVNVRVIAATHRDLRAEVNA
CCCCEEEHHHHCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEEECCHHHHHHHHH
ARFRSDLFFRLAVVRIIIPALRERPEDIPLIAERILMAFGAGSEQLAALSTPEFIAQLQH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHH
AAWPGNVRELRNHLERCLVFQDAMPPDAEEVSAQAVLRSLVDPKQPYAEARRRALEVFER
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EYLDALLKLHGGKVSQAATAADMDRVYLYRLLRRHRLRT
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SHTERPQGAGRPAEGPDSVQQTRPTGTGRPGTSGAVRRFRFTVLEGPQPGQSKDSNADT
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHEEEEEEECCCCCCCCCCCCCCE
FSIGSHGLNDFVIEEPTVSRFHCEVKVDHDGARLRDLDSRNGTVLDGVHVRDAYLRGGSI
EECCCCCCCCCEECCCCCCEEEEEEEECCCCCHHHCCCCCCCCEECCEEEEHHHHCCCCE
LRLGRVSVRFDFSPETNRLLISDRTTFGELVGSSPVTRASFALMERAAASDATVLLEGET
EEEEEEEEEEECCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCC
GTGKSRAAFAIHQASARASGPFLTVDCGAIPGNLLESELFGHEKGSFTGAIQRRVGAFEE
CCCCCHHEEEEEHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCC
ADGGTIFLDEIGELPAELQPKLLRVLENKEIRRLGANGYQPVNVRVIAATHRDLRAEVNA
CCCCEEEHHHHCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEEECCHHHHHHHHH
ARFRSDLFFRLAVVRIIIPALRERPEDIPLIAERILMAFGAGSEQLAALSTPEFIAQLQH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHH
AAWPGNVRELRNHLERCLVFQDAMPPDAEEVSAQAVLRSLVDPKQPYAEARRRALEVFER
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EYLDALLKLHGGKVSQAATAADMDRVYLYRLLRRHRLRT
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]