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The map label for this gene is sbcD [H]

Identifier: 108759333

GI number: 108759333

Start: 1102076

End: 1103320

Strand: Direct

Name: sbcD [H]

Synonym: MXAN_0958

Alternate gene names: 108759333

Gene position: 1102076-1103320 (Clockwise)

Preceding gene: 108762615

Following gene: 108756879

Centisome position: 12.06

GC content: 71.97

Gene sequence:

>1245_bases
ATGCGCCTGCTGCACACGTCGGACTGGCACCTGGGACACACGCTGTACGACGTCTCGCGGGATGCGGAGCACGCGGCCTT
CCTGGACTGGCTGCTGGAGACCCTGGAAGCGCAGTCCGTGGATGCGCTGCTGGTGGCCGGAGACATCTTCGACACGGCGA
ACCCCAGCGCGGAGGCGCAGGCGGCCTGGTACCAGTTCGTCGCGAAGGCGCGGCGGCGGATGCCCCGGCTGGACGTGGTG
GTGATTGGCGGCAACCACGACTCCGCGGCGCGGCTGGACGCGCCGGACCCGCTGTTCGCGGCGCTGGGCGTGCACGTGGT
GGGCGGACTGCCGCGGGCCCGAGGCGCCATGGACCAGGAGCGGCTGCTGGTGCCGCTGCACGACGCGAAGGGACGGGTGG
GTGCGTGGGTGGCGGCGGTGCCGTACCTGCGTCCCGCGGACCTGCCGCCCGTGCCCGGGGACATGGGAGACCGGCTGGTG
GAGGGTGTGCGCTCGGTGTACGCGGAGGTGCTGGCCGCGGCGCGGCGCAGGCGTCAGGCGGGACAGGCGCTGGTGGCCAT
GGGGCATTGCTACATGACGGGCACGGAGCTGTCCGCGTTGAGCGAGCGGAAGATTCTCGGCGGCAACCAGCACGCGCTGC
CCGTGGACCTGTTCCCGGACGACGTGGCCTACGCGGCGCTGGGGCATCTGCACAAGGCCCAGCGGGTGGGCGGACGCGAG
GGGGTGCGTTACAGCGGCTCGCCGTTGCCCCTGTCGCTGTCGGAGGCGGGCTACCGGCATCAGGTGCTGCTGGTGGAGCT
GGAAGGCGACGCGCTGCGCGAGGTGCAATCCCTGCCCGTGCCCCGGACCACGGACATGGTGCGCGTGCCTTCGCGGGACG
CTGCTCCCTTGGACGCGGTGCTGACGCAACTGGAGGGCTTGCCGGCGCACGACGCGGACACGCTGGAGTGGCGGCGGCCC
TACCTGGAGGTGTGCGTGTCGTTGCCCCGGCCGGAGCCCGCGCTGCGGCAGAAGGTGGAGAAGGTGCTGGAGGGGAAGGC
GGCGCGGCTGGTGAAGCTGACGCCCGCCTATACCGGGACGGGAGGCGCGTTGGCGGAGGTGCAGCCGGGCCTCTCCCTGA
AGGAACGCACGCCCGAGGATGTCTTCCGCGCGCGTTACGCCCGTGACTTCGAGGAGCCACCCGCACTGGCGCTGCTGGAG
GCCTTTCATACGTTGCTGACCGACGTGCAGGAGGAGTCGCCGTGA

Upstream 100 bases:

>100_bases
AGTAAGGGAGCAGGCGCCCGCCGCTTGGAGCGAGCAGCCCCTCTGTTGGAGGCTGCCCGCTGGCCAGAGCGCCACGGGAA
AGAGGGAGGGTGGACGCCTG

Downstream 100 bases:

>100_bases
AGGTGCTCGCGATTCGCGGTTCCAACCTGACGAGCTTCGCGGGGGACTTCGCGCTGGAGCTGGACCAGCCGCCGTTGGAC
CGGCTGGGCTTGTTCGCGAT

Product: nuclease SbcCD subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRLLHTSDWHLGHTLYDVSRDAEHAAFLDWLLETLEAQSVDALLVAGDIFDTANPSAEAQAAWYQFVAKARRRMPRLDVV
VIGGNHDSAARLDAPDPLFAALGVHVVGGLPRARGAMDQERLLVPLHDAKGRVGAWVAAVPYLRPADLPPVPGDMGDRLV
EGVRSVYAEVLAAARRRRQAGQALVAMGHCYMTGTELSALSERKILGGNQHALPVDLFPDDVAYAALGHLHKAQRVGGRE
GVRYSGSPLPLSLSEAGYRHQVLLVELEGDALREVQSLPVPRTTDMVRVPSRDAAPLDAVLTQLEGLPAHDADTLEWRRP
YLEVCVSLPRPEPALRQKVEKVLEGKAARLVKLTPAYTGTGGALAEVQPGLSLKERTPEDVFRARYARDFEEPPALALLE
AFHTLLTDVQEESP

Sequences:

>Translated_414_residues
MRLLHTSDWHLGHTLYDVSRDAEHAAFLDWLLETLEAQSVDALLVAGDIFDTANPSAEAQAAWYQFVAKARRRMPRLDVV
VIGGNHDSAARLDAPDPLFAALGVHVVGGLPRARGAMDQERLLVPLHDAKGRVGAWVAAVPYLRPADLPPVPGDMGDRLV
EGVRSVYAEVLAAARRRRQAGQALVAMGHCYMTGTELSALSERKILGGNQHALPVDLFPDDVAYAALGHLHKAQRVGGRE
GVRYSGSPLPLSLSEAGYRHQVLLVELEGDALREVQSLPVPRTTDMVRVPSRDAAPLDAVLTQLEGLPAHDADTLEWRRP
YLEVCVSLPRPEPALRQKVEKVLEGKAARLVKLTPAYTGTGGALAEVQPGLSLKERTPEDVFRARYARDFEEPPALALLE
AFHTLLTDVQEESP
>Mature_414_residues
MRLLHTSDWHLGHTLYDVSRDAEHAAFLDWLLETLEAQSVDALLVAGDIFDTANPSAEAQAAWYQFVAKARRRMPRLDVV
VIGGNHDSAARLDAPDPLFAALGVHVVGGLPRARGAMDQERLLVPLHDAKGRVGAWVAAVPYLRPADLPPVPGDMGDRLV
EGVRSVYAEVLAAARRRRQAGQALVAMGHCYMTGTELSALSERKILGGNQHALPVDLFPDDVAYAALGHLHKAQRVGGRE
GVRYSGSPLPLSLSEAGYRHQVLLVELEGDALREVQSLPVPRTTDMVRVPSRDAAPLDAVLTQLEGLPAHDADTLEWRRP
YLEVCVSLPRPEPALRQKVEKVLEGKAARLVKLTPAYTGTGGALAEVQPGLSLKERTPEDVFRARYARDFEEPPALALLE
AFHTLLTDVQEESP

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=309, Percent_Identity=38.8349514563107, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 45133; Mature: 45133

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTSDWHLGHTLYDVSRDAEHAAFLDWLLETLEAQSVDALLVAGDIFDTANPSAEAQ
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHCCCCCCCHHH
AAWYQFVAKARRRMPRLDVVVIGGNHDSAARLDAPDPLFAALGVHVVGGLPRARGAMDQE
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCCC
RLLVPLHDAKGRVGAWVAAVPYLRPADLPPVPGDMGDRLVEGVRSVYAEVLAAARRRRQA
CEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GQALVAMGHCYMTGTELSALSERKILGGNQHALPVDLFPDDVAYAALGHLHKAQRVGGRE
HHHHHHHHHHEECCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCC
GVRYSGSPLPLSLSEAGYRHQVLLVELEGDALREVQSLPVPRTTDMVRVPSRDAAPLDAV
CCCCCCCCCCEECCCCCCCEEEEEEEECCHHHHHHHHCCCCCCCCEEECCCCCCCCHHHH
LTQLEGLPAHDADTLEWRRPYLEVCVSLPRPEPALRQKVEKVLEGKAARLVKLTPAYTGT
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
GGALAEVQPGLSLKERTPEDVFRARYARDFEEPPALALLEAFHTLLTDVQEESP
CCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLLHTSDWHLGHTLYDVSRDAEHAAFLDWLLETLEAQSVDALLVAGDIFDTANPSAEAQ
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHCCCCCCCHHH
AAWYQFVAKARRRMPRLDVVVIGGNHDSAARLDAPDPLFAALGVHVVGGLPRARGAMDQE
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCCC
RLLVPLHDAKGRVGAWVAAVPYLRPADLPPVPGDMGDRLVEGVRSVYAEVLAAARRRRQA
CEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GQALVAMGHCYMTGTELSALSERKILGGNQHALPVDLFPDDVAYAALGHLHKAQRVGGRE
HHHHHHHHHHEECCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCC
GVRYSGSPLPLSLSEAGYRHQVLLVELEGDALREVQSLPVPRTTDMVRVPSRDAAPLDAV
CCCCCCCCCCEECCCCCCCEEEEEEEECCHHHHHHHHCCCCCCCCEEECCCCCCCCHHHH
LTQLEGLPAHDADTLEWRRPYLEVCVSLPRPEPALRQKVEKVLEGKAARLVKLTPAYTGT
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
GGALAEVQPGLSLKERTPEDVFRARYARDFEEPPALALLEAFHTLLTDVQEESP
CCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9256491 [H]