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The map label for this gene is yfkN [H]

Identifier: 108759234

GI number: 108759234

Start: 5312926

End: 5314524

Strand: Reverse

Name: yfkN [H]

Synonym: MXAN_4313

Alternate gene names: 108759234

Gene position: 5314524-5312926 (Counterclockwise)

Preceding gene: 108759677

Following gene: 108758020

Centisome position: 58.15

GC content: 71.17

Gene sequence:

>1599_bases
ATGTCCCGTCTCCTGTCCGCGCTCGGCGCGCTGCTCGTCCTGGCCTCAGGCTGTGCTTCATCCCGGCCTCCGGTCACCGC
CAGCGCTCCCACGTCTCAGCGCCAGCAGCTCACCGTCATCTACGTGGCGGACCTGCACGCGCAACTGCGCGCCCACCCGG
AGCTCTTCTGGAACGATGGGAAGGAGCGCATCGAGATGGCGGGCGGATTCGCCCGCGTCGCCGCCGCCATCCAACAGATT
CGGGCCGAGCGCGGTGGTGACGTGCTGGTGCTCGACGCCGGCGACACGATTCAAGGTTCGGGCGCGGCTGCTCTCACCGA
GGGCGGCGTCCTCATCGAACCGCTCAATGCGCTCGGGCTGGACGGCGCGGTGCCCGGCAACTGGGAGGTGGTGTACGGCC
CCGCGGTGCTGCGCCAGCGCGCGCGTGAGCTGAAGCATCCGCTCTTCGCTGCCAACCTGCGTGACGCGGCCAGCGGTGAA
CGGCTCTTCCCGCCGTACCTGGTGAAGGAGGTGGGCGGGGTGAAGGTGGCCGTGGTGGGCTTCACGGACCCGGACGTCCC
GCGCCGTCAGCCCCCGGGGTACAGCAAGGGGCTGCGCTACGACGGCCCGGAGGCGCTGCCGGAGCTGGTGCGCGAGGTGC
GCGAGCGCGAGGGCGCCCAGGTGGTGCTCTTGATGTCGCACGTGGGGCTCGCCAAGGCCGTGGGGATCGCCGCCCGGGTG
CCGGGCGTGGACGCCCACCTGTCCAGCGACACGCATGAGCGCACCTATGTCCCCATCGAGCAGGGCGGAAGCTGGGTGGT
GGAGCCCGGCGCCTTCGGCTCCTTCCTCGGCCGGATGGACTTGTGGCTGGAGGACGGGAAGGTGGTGGACCGCCGCTGGG
AGCTCATCGAGCTGACCGCGTCGCGCTTCCCCGAGGACCCGGAGATGGCGCGGCTCGTGGACGCCGCGCTCGCGCCTTAT
GACGAGCAGCTGTCCTCGCCGGTGGGGCACACCGACGTGACGCTCGCGCGCTACGCGGTGGTGGAGAACCCGCTGGACAA
CGTGCTGGCTGACGCCATCCGCGCCGCTGGCGGGACGGAGATTGGCCTCTCCAACGGCTTCCGCTTCGGCACGCCGCTGC
TGCCCGGGCCCGTGCGTGAGGCGGACCTGTGGAACTTCTTTCCCATCACCAACAAGCTGAAGACGGGCAAGGTGAGTGGC
CGTCAGCTTCGCGCCTTCTGGGAGCAGGAGCTGGAGAACGTTTTCGCGAAGGACCCGGAGAAGCGCTTCGGCGGGTGGCT
GCCGCGCCCCTCGGGGATGACGGTGCGCTTCCGCTCGGAGGCCCCCAAGGGGCAGCGCCTCCTCGCCCTGGAGGTCGCTG
GCGAGCCGGTGGTGGATGAGCGCCTCTACACCGTGACGGCCTGCGAGCGGGAGGGGGACTCGCCGGACATGCTCTGCCGC
ATCCCCGGGGTCCAGGATCCGCAGGTGCTCGACATGGACGCGCACGAGGCGGTGCGCCGCTTCCTCGCTGGCAAGCCGCG
ACTGCGCGAGTCGCTGGAGGGCCGCGCGGTGGGCCTGGACCTGCCGGCCGTGCTCCGCACCCAGCAGGTTCAACCGTGA

Upstream 100 bases:

>100_bases
TGCGCGAGGCGTTCGAGGTGAGCCGCCGTCCGGGAACTTCGCGCGGCGCACCTCGTGTCACTTCCGCTGTCTCCTTTCAC
TCCTCGCAAGGTGCTCCACG

Downstream 100 bases:

>100_bases
GCGCGACTTCCCCCCACCGTCCCCCTGAAATGACTGCACCTCTCGCCACTTCCGACTCGATTTCCGCCGCGCCTCCGCCT
GGAGGCCCCTGGCGCCGGCG

Product: 5`-nucleotidase family protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MSRLLSALGALLVLASGCASSRPPVTASAPTSQRQQLTVIYVADLHAQLRAHPELFWNDGKERIEMAGGFARVAAAIQQI
RAERGGDVLVLDAGDTIQGSGAAALTEGGVLIEPLNALGLDGAVPGNWEVVYGPAVLRQRARELKHPLFAANLRDAASGE
RLFPPYLVKEVGGVKVAVVGFTDPDVPRRQPPGYSKGLRYDGPEALPELVREVREREGAQVVLLMSHVGLAKAVGIAARV
PGVDAHLSSDTHERTYVPIEQGGSWVVEPGAFGSFLGRMDLWLEDGKVVDRRWELIELTASRFPEDPEMARLVDAALAPY
DEQLSSPVGHTDVTLARYAVVENPLDNVLADAIRAAGGTEIGLSNGFRFGTPLLPGPVREADLWNFFPITNKLKTGKVSG
RQLRAFWEQELENVFAKDPEKRFGGWLPRPSGMTVRFRSEAPKGQRLLALEVAGEPVVDERLYTVTACEREGDSPDMLCR
IPGVQDPQVLDMDAHEAVRRFLAGKPRLRESLEGRAVGLDLPAVLRTQQVQP

Sequences:

>Translated_532_residues
MSRLLSALGALLVLASGCASSRPPVTASAPTSQRQQLTVIYVADLHAQLRAHPELFWNDGKERIEMAGGFARVAAAIQQI
RAERGGDVLVLDAGDTIQGSGAAALTEGGVLIEPLNALGLDGAVPGNWEVVYGPAVLRQRARELKHPLFAANLRDAASGE
RLFPPYLVKEVGGVKVAVVGFTDPDVPRRQPPGYSKGLRYDGPEALPELVREVREREGAQVVLLMSHVGLAKAVGIAARV
PGVDAHLSSDTHERTYVPIEQGGSWVVEPGAFGSFLGRMDLWLEDGKVVDRRWELIELTASRFPEDPEMARLVDAALAPY
DEQLSSPVGHTDVTLARYAVVENPLDNVLADAIRAAGGTEIGLSNGFRFGTPLLPGPVREADLWNFFPITNKLKTGKVSG
RQLRAFWEQELENVFAKDPEKRFGGWLPRPSGMTVRFRSEAPKGQRLLALEVAGEPVVDERLYTVTACEREGDSPDMLCR
IPGVQDPQVLDMDAHEAVRRFLAGKPRLRESLEGRAVGLDLPAVLRTQQVQP
>Mature_531_residues
SRLLSALGALLVLASGCASSRPPVTASAPTSQRQQLTVIYVADLHAQLRAHPELFWNDGKERIEMAGGFARVAAAIQQIR
AERGGDVLVLDAGDTIQGSGAAALTEGGVLIEPLNALGLDGAVPGNWEVVYGPAVLRQRARELKHPLFAANLRDAASGER
LFPPYLVKEVGGVKVAVVGFTDPDVPRRQPPGYSKGLRYDGPEALPELVREVREREGAQVVLLMSHVGLAKAVGIAARVP
GVDAHLSSDTHERTYVPIEQGGSWVVEPGAFGSFLGRMDLWLEDGKVVDRRWELIELTASRFPEDPEMARLVDAALAPYD
EQLSSPVGHTDVTLARYAVVENPLDNVLADAIRAAGGTEIGLSNGFRFGTPLLPGPVREADLWNFFPITNKLKTGKVSGR
QLRAFWEQELENVFAKDPEKRFGGWLPRPSGMTVRFRSEAPKGQRLLALEVAGEPVVDERLYTVTACEREGDSPDMLCRI
PGVQDPQVLDMDAHEAVRRFLAGKPRLRESLEGRAVGLDLPAVLRTQQVQP

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=357, Percent_Identity=26.6106442577031, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1786687, Length=552, Percent_Identity=22.1014492753623, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1790658, Length=384, Percent_Identity=25.2604166666667, Blast_Score=82, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19922446, Length=513, Percent_Identity=24.9512670565302, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24654424, Length=513, Percent_Identity=24.9512670565302, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24652512, Length=418, Percent_Identity=25.8373205741627, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19921980, Length=418, Percent_Identity=25.8373205741627, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI161076508, Length=418, Percent_Identity=25.8373205741627, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI28573524, Length=425, Percent_Identity=23.0588235294118, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 57723; Mature: 57591

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLLSALGALLVLASGCASSRPPVTASAPTSQRQQLTVIYVADLHAQLRAHPELFWNDG
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHCCCCCEECCC
KERIEMAGGFARVAAAIQQIRAERGGDVLVLDAGDTIQGSGAAALTEGGVLIEPLNALGL
HHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCEEEECHHHCCC
DGAVPGNWEVVYGPAVLRQRARELKHPLFAANLRDAASGERLFPPYLVKEVGGVKVAVVG
CCCCCCCCEEEECHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHCCCEEEEEEE
FTDPDVPRRQPPGYSKGLRYDGPEALPELVREVREREGAQVVLLMSHVGLAKAVGIAARV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHCC
PGVDAHLSSDTHERTYVPIEQGGSWVVEPGAFGSFLGRMDLWLEDGKVVDRRWELIELTA
CCCCCCCCCCCCCCEEEEEECCCCEEECCCCHHHHHHHHHEEECCCEEEHHHHHHHHHHH
SRFPEDPEMARLVDAALAPYDEQLSSPVGHTDVTLARYAVVENPLDNVLADAIRAAGGTE
HHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCE
IGLSNGFRFGTPLLPGPVREADLWNFFPITNKLKTGKVSGRQLRAFWEQELENVFAKDPE
EECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
KRFGGWLPRPSGMTVRFRSEAPKGQRLLALEVAGEPVVDERLYTVTACEREGDSPDMLCR
HHHCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEE
IPGVQDPQVLDMDAHEAVRRFLAGKPRLRESLEGRAVGLDLPAVLRTQQVQP
CCCCCCCCEECCCHHHHHHHHHCCCHHHHHHCCCCEEECCCHHHHHHCCCCC
>Mature Secondary Structure 
SRLLSALGALLVLASGCASSRPPVTASAPTSQRQQLTVIYVADLHAQLRAHPELFWNDG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHCCCCCEECCC
KERIEMAGGFARVAAAIQQIRAERGGDVLVLDAGDTIQGSGAAALTEGGVLIEPLNALGL
HHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCEEEECHHHCCC
DGAVPGNWEVVYGPAVLRQRARELKHPLFAANLRDAASGERLFPPYLVKEVGGVKVAVVG
CCCCCCCCEEEECHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHCCCEEEEEEE
FTDPDVPRRQPPGYSKGLRYDGPEALPELVREVREREGAQVVLLMSHVGLAKAVGIAARV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHCC
PGVDAHLSSDTHERTYVPIEQGGSWVVEPGAFGSFLGRMDLWLEDGKVVDRRWELIELTA
CCCCCCCCCCCCCCEEEEEECCCCEEECCCCHHHHHHHHHEEECCCEEEHHHHHHHHHHH
SRFPEDPEMARLVDAALAPYDEQLSSPVGHTDVTLARYAVVENPLDNVLADAIRAAGGTE
HHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCE
IGLSNGFRFGTPLLPGPVREADLWNFFPITNKLKTGKVSGRQLRAFWEQELENVFAKDPE
EECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
KRFGGWLPRPSGMTVRFRSEAPKGQRLLALEVAGEPVVDERLYTVTACEREGDSPDMLCR
HHHCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEE
IPGVQDPQVLDMDAHEAVRRFLAGKPRLRESLEGRAVGLDLPAVLRTQQVQP
CCCCCCCCEECCCHHHHHHHHHCCCHHHHHHCCCCEEECCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]