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The map label for this gene is thiE

Identifier: 108759139

GI number: 108759139

Start: 6359874

End: 6360530

Strand: Reverse

Name: thiE

Synonym: MXAN_5087

Alternate gene names: 108759139

Gene position: 6360530-6359874 (Counterclockwise)

Preceding gene: 108762358

Following gene: 108762652

Centisome position: 69.59

GC content: 72.15

Gene sequence:

>657_bases
ATGAACGCACCGTCCCGCCCGCACCTTCCCCGCGGTCCTTACCTGCTGTGCGACGACTCCGTCCTGCCGGAGATTTCACT
GGTGGACAAGGCGGCTCGCCTGGTGGCCGGCGGGGCCCGGCTGGTGCAACTGCGCATGAAGCGCACCCCGATTCGTGAGG
CGCTGGCCGCCACGCGGCAGGTGGTGGCGCTGTGCCGCCGGGAAGGCGCGCTGTGCCTGGTGAACGACCGGGTGGACCTG
GCGCTGCTGGCGGACGCGGACGGTGTGCACGTGGGTGACGAGGACGTGCCCGCCGAGGATGCGCGCGCGCTGCTGGGGCC
TGGCCGTCTGGTGGGCGTCACGGTGCGAGACGTGGTGGGCGCGCGGGCGGCGCAAGCGGCGGGGGCGGACTATGTGGGGC
TGGGGCCTGTCTTCCCCACGTCGACGAAACAGGTGCCCGCGCCAGTGCTGGGTCTGGAGGCCTTCGCGTCCGTGGTGCGT
GACAGCCCGCTGCCCGTGGTGGGCATTGGTGGGGTGGGGCTGGTGAACATCGCGAGCGTGGCGGCGGCCGGGGCGCACTG
TGCGGCGGTGGTGTCCGACGCGCTGCTCGCCGCGGATATCACCGAGCGTGTGAGGCGGTTGGTTGAGGCGTTTGAACAGG
GGCGTTTCGGGGCATAG

Upstream 100 bases:

>100_bases
TCCGCCATGGTTCAAATCCTCAGAATTGCTTGGAAAACAGCGCGGCACCTTATAGCAGGGTGGCCATCTGTCAACGCCGC
CGTCCTGCCTTCCAGAGCCG

Downstream 100 bases:

>100_bases
CCTCGAAGCCAGGAGCTTCCATGAACAACCATTCGCGTCACCACGGGCCGGCGCCGCGCCGCCCCAACATCGGCATCACC
CCGGACTGGAGTCCGGCCGG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MNAPSRPHLPRGPYLLCDDSVLPEISLVDKAARLVAGGARLVQLRMKRTPIREALAATRQVVALCRREGALCLVNDRVDL
ALLADADGVHVGDEDVPAEDARALLGPGRLVGVTVRDVVGARAAQAAGADYVGLGPVFPTSTKQVPAPVLGLEAFASVVR
DSPLPVVGIGGVGLVNIASVAAAGAHCAAVVSDALLAADITERVRRLVEAFEQGRFGA

Sequences:

>Translated_218_residues
MNAPSRPHLPRGPYLLCDDSVLPEISLVDKAARLVAGGARLVQLRMKRTPIREALAATRQVVALCRREGALCLVNDRVDL
ALLADADGVHVGDEDVPAEDARALLGPGRLVGVTVRDVVGARAAQAAGADYVGLGPVFPTSTKQVPAPVLGLEAFASVVR
DSPLPVVGIGGVGLVNIASVAAAGAHCAAVVSDALLAADITERVRRLVEAFEQGRFGA
>Mature_218_residues
MNAPSRPHLPRGPYLLCDDSVLPEISLVDKAARLVAGGARLVQLRMKRTPIREALAATRQVVALCRREGALCLVNDRVDL
ALLADADGVHVGDEDVPAEDARALLGPGRLVGVTVRDVVGARAAQAAGADYVGLGPVFPTSTKQVPAPVLGLEAFASVVR
DSPLPVVGIGGVGLVNIASVAAAGAHCAAVVSDALLAADITERVRRLVEAFEQGRFGA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=205, Percent_Identity=37.0731707317073, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6325042, Length=224, Percent_Identity=33.4821428571429, Blast_Score=98, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_MYXXD (Q1D282)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_633241.1
- ProteinModelPortal:   Q1D282
- SMR:   Q1D282
- STRING:   Q1D282
- GeneID:   4102751
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_5087
- TIGR:   MXAN_5087
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   QRDGADY
- PhylomeDB:   Q1D282
- ProtClustDB:   PRK00043
- BioCyc:   MXAN246197:MXAN_5087-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22471; Mature: 22471

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPSRPHLPRGPYLLCDDSVLPEISLVDKAARLVAGGARLVQLRMKRTPIREALAATRQ
CCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHH
VVALCRREGALCLVNDRVDLALLADADGVHVGDEDVPAEDARALLGPGRLVGVTVRDVVG
HHHHHHCCCCEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHH
ARAAQAAGADYVGLGPVFPTSTKQVPAPVLGLEAFASVVRDSPLPVVGIGGVGLVNIASV
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHH
AAAGAHCAAVVSDALLAADITERVRRLVEAFEQGRFGA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNAPSRPHLPRGPYLLCDDSVLPEISLVDKAARLVAGGARLVQLRMKRTPIREALAATRQ
CCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHH
VVALCRREGALCLVNDRVDLALLADADGVHVGDEDVPAEDARALLGPGRLVGVTVRDVVG
HHHHHHCCCCEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHH
ARAAQAAGADYVGLGPVFPTSTKQVPAPVLGLEAFASVVRDSPLPVVGIGGVGLVNIASV
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHH
AAAGAHCAAVVSDALLAADITERVRRLVEAFEQGRFGA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA