Click here to switch to the map view.

The map label for this gene is polA [H]

Identifier: 108759097

GI number: 108759097

Start: 391581

End: 392465

Strand: Direct

Name: polA [H]

Synonym: MXAN_0335

Alternate gene names: 108759097

Gene position: 391581-392465 (Clockwise)

Preceding gene: 108759209

Following gene: 108761908

Centisome position: 4.28

GC content: 72.32

Gene sequence:

>885_bases
ATGCGCCTGCACCTGGTCGACGGCACCTACGAGCTCTACCGCGCCCATTTCTCGCCCCGTCCGGGCCATGCCGCGCCGGA
TGGACAGGACGTGAAGGCCACGGTGGGGCTGATGTCCTCGCTGCTCGCGCTGCTGCACGACGCGGACGAGGCGGTGACGC
ACGTGGCGGTGGCGTTCGACAACCCCATCCGCTCGTTCCGCAACGCGCTGTTCGACGGCTACAAGAGCGACGAGGGCGTG
CCCCCGGAGCTGCACGCGCAGTTCGACCTGGCGGAGGAGGCCGTGCGCGCGCTGGGCGTCACCGCGTGGTCCATGAAGGA
GCAGGAGGCGGATGACGCGCTGGCCACCGCCGCGGCGCGCTGGGCGGACACGGTGGAGCAGGTGCGGCTGCTCACGCCCG
ACAAGGACTTGGGCCAGTGCGTGCGTGGCAGCCGTGTGGTGCAGGTGGACCGGCGGCAGGAAAAGGTGCTCGACGAGGAC
GCAGTGCGCGCGAAGCTGGGCGTGCCCCCCGCGAGCGTGCCGGACCTGCTGGCGCTGATGGGCGACGCGGCGGATGGCAT
CCCCGGACTGCCGGGCTTCGGGGAGAAGGGCGCGTCGGCATTGCTGAGCGCGCATGGCCACCTGGAGGACATTCCCGCGG
AGGCGTCCGAGTGGAAGGTGCGTCCCCGGGGCGCGGAGAAGCTGGCAGCGACGCTGCGCGAGCACCGTGACGCCGCGCTG
CTCTACCGCCGGCTGGCCACGCTGGTGACGGACGCGCCGCTGCCGGGCACGTCGTCACTGGAGGACCTGGCGTGGAAGGG
CGTGCCGCCCGCCGTGTACGAAGCGTTCTGTGACCGGCTGGGCCTGACGACGCTCAAGCGCCGTCCCAAGCGCTGGGCGC
CGTGA

Upstream 100 bases:

>100_bases
TGGCAGGGTTGGCCGAAGGGCGGGCGAGCAGCCTTGCAAGCGCCTTCCGGGCGCACCGGGGCACTGGCCGGCGTGCGCCG
CCGGGGGTAGAGAGGGCGAC

Downstream 100 bases:

>100_bases
GCTCGTGCAGGGGCAGCGCCGTGGGCGCATGGCGTGGCAGCCGCACGGTGAAGGTGGTGCCCGTCTCCTCCTGGGAGCGG
GCCTCCACCGTGCCGCCGTG

Product: 5'-3' exonuclease family protein

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MRLHLVDGTYELYRAHFSPRPGHAAPDGQDVKATVGLMSSLLALLHDADEAVTHVAVAFDNPIRSFRNALFDGYKSDEGV
PPELHAQFDLAEEAVRALGVTAWSMKEQEADDALATAAARWADTVEQVRLLTPDKDLGQCVRGSRVVQVDRRQEKVLDED
AVRAKLGVPPASVPDLLALMGDAADGIPGLPGFGEKGASALLSAHGHLEDIPAEASEWKVRPRGAEKLAATLREHRDAAL
LYRRLATLVTDAPLPGTSSLEDLAWKGVPPAVYEAFCDRLGLTTLKRRPKRWAP

Sequences:

>Translated_294_residues
MRLHLVDGTYELYRAHFSPRPGHAAPDGQDVKATVGLMSSLLALLHDADEAVTHVAVAFDNPIRSFRNALFDGYKSDEGV
PPELHAQFDLAEEAVRALGVTAWSMKEQEADDALATAAARWADTVEQVRLLTPDKDLGQCVRGSRVVQVDRRQEKVLDED
AVRAKLGVPPASVPDLLALMGDAADGIPGLPGFGEKGASALLSAHGHLEDIPAEASEWKVRPRGAEKLAATLREHRDAAL
LYRRLATLVTDAPLPGTSSLEDLAWKGVPPAVYEAFCDRLGLTTLKRRPKRWAP
>Mature_294_residues
MRLHLVDGTYELYRAHFSPRPGHAAPDGQDVKATVGLMSSLLALLHDADEAVTHVAVAFDNPIRSFRNALFDGYKSDEGV
PPELHAQFDLAEEAVRALGVTAWSMKEQEADDALATAAARWADTVEQVRLLTPDKDLGQCVRGSRVVQVDRRQEKVLDED
AVRAKLGVPPASVPDLLALMGDAADGIPGLPGFGEKGASALLSAHGHLEDIPAEASEWKVRPRGAEKLAATLREHRDAAL
LYRRLATLVTDAPLPGTSSLEDLAWKGVPPAVYEAFCDRLGLTTLKRRPKRWAP

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=259, Percent_Identity=37.0656370656371, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI226510970, Length=215, Percent_Identity=31.6279069767442, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 31867; Mature: 31867

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLHLVDGTYELYRAHFSPRPGHAAPDGQDVKATVGLMSSLLALLHDADEAVTHVAVAFD
CEEEEECCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPIRSFRNALFDGYKSDEGVPPELHAQFDLAEEAVRALGVTAWSMKEQEADDALATAAAR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WADTVEQVRLLTPDKDLGQCVRGSRVVQVDRRQEKVLDEDAVRAKLGVPPASVPDLLALM
HHHHHHHHHHCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
GDAADGIPGLPGFGEKGASALLSAHGHLEDIPAEASEWKVRPRGAEKLAATLREHRDAAL
HCCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHHH
LYRRLATLVTDAPLPGTSSLEDLAWKGVPPAVYEAFCDRLGLTTLKRRPKRWAP
HHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHCHHHHHHCCCCCCC
>Mature Secondary Structure
MRLHLVDGTYELYRAHFSPRPGHAAPDGQDVKATVGLMSSLLALLHDADEAVTHVAVAFD
CEEEEECCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPIRSFRNALFDGYKSDEGVPPELHAQFDLAEEAVRALGVTAWSMKEQEADDALATAAAR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WADTVEQVRLLTPDKDLGQCVRGSRVVQVDRRQEKVLDEDAVRAKLGVPPASVPDLLALM
HHHHHHHHHHCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
GDAADGIPGLPGFGEKGASALLSAHGHLEDIPAEASEWKVRPRGAEKLAATLREHRDAAL
HCCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHHH
LYRRLATLVTDAPLPGTSSLEDLAWKGVPPAVYEAFCDRLGLTTLKRRPKRWAP
HHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486614 [H]