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The map label for this gene is trmJ [H]

Identifier: 108759067

GI number: 108759067

Start: 2416036

End: 2416767

Strand: Direct

Name: trmJ [H]

Synonym: MXAN_2082

Alternate gene names: 108759067

Gene position: 2416036-2416767 (Clockwise)

Preceding gene: 108758602

Following gene: 108758136

Centisome position: 26.43

GC content: 67.49

Gene sequence:

>732_bases
ATGCGGCCAGGGGAGCAGCTTACCGTTGTCCTTCATCAGACGCGTTCAACCGACAACCTCGGAGCCGTGGCCAGAATCAT
GGCCAACTTCGGGTACTCACGGCTGATTTTGTCCGAACCGGTGCTGAAGTCGATCGAGGGCGCGGAGCGTCTCGCGGTCA
AGAGTGACTTCGTCCTACGAGGCATGGAGGTGACGTCCGACCTGGGCGAAGCGCTGAAGGACTGCGTGTACGCGGTGGGC
ACGACTTCTCGTACCCAGCTCAAGGGACGCACCGTGCTGACGCCGGAGGAGGCCATGCAGCGGCTGGCGGAGGAGAGCGT
GCGCGGACGCGTGGCGCTCGTCTTCGGTGGCGAGCAGCGCGGCATGTCCGACGAGGACCTGGCGCGGTGCACGGACGTGC
TCGCGATTCCCACGTCGGCGGTGCAGCCGTCCATGAACCTGGCGCAGTCGTCAGCGGTGCTGCTGTACCTCTGTCACCGT
CAGGGGCTGACGCCCACGGCCGCGGTGGAGGAGGCGCCAGGCGCGAAGCTGGGCACGCTGGGGGCGCTGGCGAAGCGAAT
GAGGGACGTCATGCTGCGCGCGGAGTTCCTCAACCCGCAGGCGCCGCAGCATGTGCTGAACGAGCTGGAGCAGACGTTGA
TGCGTGCGCGGCTGACCCAGCGCGAGGCGGAGCTGTGGCTCACGGCCTTCAAGCACCTGGAGCGCGCGGTGACGCGGGGC
GCCTCTACCTGA

Upstream 100 bases:

>100_bases
TGAGGACCGTCACGAGAGAGAGGAGCAACCCAGGGAGCCAGCGACGCATCAACCGGGAATATACCGATCTGCTTCGGGAT
CAACCAGCTAAACTCAGCGC

Downstream 100 bases:

>100_bases
GGAAGAGGCCGCCCCGGCGCCGACGGCGAGTCGCGGTCTACGCCGCGTCCTTCTTGAAGGCCTCGCCGTGGTGCTTCTCG
CACAGGCCGCCCTTGAACGT

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRPGEQLTVVLHQTRSTDNLGAVARIMANFGYSRLILSEPVLKSIEGAERLAVKSDFVLRGMEVTSDLGEALKDCVYAVG
TTSRTQLKGRTVLTPEEAMQRLAEESVRGRVALVFGGEQRGMSDEDLARCTDVLAIPTSAVQPSMNLAQSSAVLLYLCHR
QGLTPTAAVEEAPGAKLGTLGALAKRMRDVMLRAEFLNPQAPQHVLNELEQTLMRARLTQREAELWLTAFKHLERAVTRG
AST

Sequences:

>Translated_243_residues
MRPGEQLTVVLHQTRSTDNLGAVARIMANFGYSRLILSEPVLKSIEGAERLAVKSDFVLRGMEVTSDLGEALKDCVYAVG
TTSRTQLKGRTVLTPEEAMQRLAEESVRGRVALVFGGEQRGMSDEDLARCTDVLAIPTSAVQPSMNLAQSSAVLLYLCHR
QGLTPTAAVEEAPGAKLGTLGALAKRMRDVMLRAEFLNPQAPQHVLNELEQTLMRARLTQREAELWLTAFKHLERAVTRG
AST
>Mature_243_residues
MRPGEQLTVVLHQTRSTDNLGAVARIMANFGYSRLILSEPVLKSIEGAERLAVKSDFVLRGMEVTSDLGEALKDCVYAVG
TTSRTQLKGRTVLTPEEAMQRLAEESVRGRVALVFGGEQRGMSDEDLARCTDVLAIPTSAVQPSMNLAQSSAVLLYLCHR
QGLTPTAAVEEAPGAKLGTLGALAKRMRDVMLRAEFLNPQAPQHVLNELEQTLMRARLTQREAELWLTAFKHLERAVTRG
AST

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=223, Percent_Identity=29.5964125560538, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790865, Length=155, Percent_Identity=33.5483870967742, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26557; Mature: 26557

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPGEQLTVVLHQTRSTDNLGAVARIMANFGYSRLILSEPVLKSIEGAERLAVKSDFVLR
CCCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMEVTSDLGEALKDCVYAVGTTSRTQLKGRTVLTPEEAMQRLAEESVRGRVALVFGGEQR
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEECHHHHHHHHHHHHHCCCEEEEECCCCC
GMSDEDLARCTDVLAIPTSAVQPSMNLAQSSAVLLYLCHRQGLTPTAAVEEAPGAKLGTL
CCCHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHH
GALAKRMRDVMLRAEFLNPQAPQHVLNELEQTLMRARLTQREAELWLTAFKHLERAVTRG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AST
CCC
>Mature Secondary Structure
MRPGEQLTVVLHQTRSTDNLGAVARIMANFGYSRLILSEPVLKSIEGAERLAVKSDFVLR
CCCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMEVTSDLGEALKDCVYAVGTTSRTQLKGRTVLTPEEAMQRLAEESVRGRVALVFGGEQR
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEECHHHHHHHHHHHHHCCCEEEEECCCCC
GMSDEDLARCTDVLAIPTSAVQPSMNLAQSSAVLLYLCHRQGLTPTAAVEEAPGAKLGTL
CCCHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHH
GALAKRMRDVMLRAEFLNPQAPQHVLNELEQTLMRARLTQREAELWLTAFKHLERAVTRG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AST
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA