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The map label for this gene is folC [H]

Identifier: 108758952

GI number: 108758952

Start: 3155372

End: 3156604

Strand: Direct

Name: folC [H]

Synonym: MXAN_2705

Alternate gene names: 108758952

Gene position: 3155372-3156604 (Clockwise)

Preceding gene: 108757925

Following gene: 108763566

Centisome position: 34.52

GC content: 71.29

Gene sequence:

>1233_bases
TTGGAGCGCGTGCAGGAGGCCCTGGCAGCGCTGGGCCACCCGGAGCGGCGGTATCCGGCGCTCCATGTCGCCGGGACGAA
CGGGAAGGGCAGCACCTGTGCCATGACGGCGGCGTCGCTCCATGCCGCCGGGCACCGCGTGGGGCTCTACACGTCTCCGC
ATCTGGTGCGCGTCAACGAGCGCATCCGCGTGGACAGCGAGGACATCTCCGACGCGGACTTCGGCCTGCGCATCCTGGAG
GTGTTGGAGCGCTACCCCAGCGCCGTGTCCTCTCCCATGACGTACTTCGAGTTCGGCACCGTCGTCGCCCTGTGGCACTT
CGCCCAGGTGCGCGTGGATGTGGCCGTGCTGGAGACGGGCCTGGGCGGCCGGCTGGACGCCACCACCGCCGCCAGCCCGG
TGGTGACGGCGATTACCCCCGTGTCCTTCGACCACATGGAGTACCTGGGCGACACGCTGGCGGCCATCGCGGGCGAGAAG
GCGGGCATCCTCAAGCCGGGCGTGCCGTGTGTCGTCGCGCGGCAGGCCCCGGAGGCCTTGGAAGCCATCCTCCGCAAGGC
CGAGCAAGTCGGGGCCCCGGTGCGGCTGGAGGGACGTGACTTCACGGGCGAGCGTCAGCCCGATGGTGGCCTGTCGTACC
GCGGGGCGACCTGGTCCCTGGATGGGCTGTCCGTCGCGCTGCGGGGGCCCCATCAGCGGCAGAACGCGGCGGTGGCGCTC
GCCTGCCTGGAGGCCTTGCAGGAGCGCGGCGTCACCGCGCCGCCCGAGGCCGCGCGTGCGGGCCTGGCGTCGGCGTGCTG
GCCGGGGCGTCTGGAGGAGGTGGGGCAGCAGCCCATCCTCTTGCTGGACGGCGCACACAACCCGGCGGGCGTGGAGGTGC
TGCTCGCGTCGCTGAAGGACCTCTACCCGGGGCGTCGCCTCCACCTCGTCTTCGGCGTGGTGGGGGACAAGGACCGGGGA
CCGATGATGCGAGCGCTGTTGCCCGAGTGTGCCTCGGTGCAGCTCACGCCTTTGGAGACGCCTCGCTCACTGGCTCCCGA
GGCCTACCTGGCAGAGGCGCGCGCGCTGTGCGCGGACGTGACTGCCTGGCCGGATGCGGACGCGGCCCTTGCCGCGGCGC
GCAAGCGGGCAGCCGCGAACGACCTCGTCCTTTGTACAGGTTCACTGTTCCTTGTCGGGATGATGAAGGCTCGCATGTAT
CGAAACCTTGGCGCGATGCATCAGCCGCCGTAG

Upstream 100 bases:

>100_bases
TGGGCCAGATCCTGGGGCTGCTGGGCTGAGCGCGCCTCGGACACCGGAGGAGGCGCTGGCCTTCTTCACGAAGCTCAACC
CCTCCGGCATCAAGCTGGGG

Downstream 100 bases:

>100_bases
ATTCAAGACATGCGCCTGCCGGACTGGAGAGCCGCCGCGACATCGGGGCCACCCATCCCCTCCATCGACGAAGTCGATTT
CAGGGCGCTTTACTCGAAGA

Product: tetrahydrofolylpolyglutamate synthase/dihydrofolate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MERVQEALAALGHPERRYPALHVAGTNGKGSTCAMTAASLHAAGHRVGLYTSPHLVRVNERIRVDSEDISDADFGLRILE
VLERYPSAVSSPMTYFEFGTVVALWHFAQVRVDVAVLETGLGGRLDATTAASPVVTAITPVSFDHMEYLGDTLAAIAGEK
AGILKPGVPCVVARQAPEALEAILRKAEQVGAPVRLEGRDFTGERQPDGGLSYRGATWSLDGLSVALRGPHQRQNAAVAL
ACLEALQERGVTAPPEAARAGLASACWPGRLEEVGQQPILLLDGAHNPAGVEVLLASLKDLYPGRRLHLVFGVVGDKDRG
PMMRALLPECASVQLTPLETPRSLAPEAYLAEARALCADVTAWPDADAALAAARKRAAANDLVLCTGSLFLVGMMKARMY
RNLGAMHQPP

Sequences:

>Translated_410_residues
MERVQEALAALGHPERRYPALHVAGTNGKGSTCAMTAASLHAAGHRVGLYTSPHLVRVNERIRVDSEDISDADFGLRILE
VLERYPSAVSSPMTYFEFGTVVALWHFAQVRVDVAVLETGLGGRLDATTAASPVVTAITPVSFDHMEYLGDTLAAIAGEK
AGILKPGVPCVVARQAPEALEAILRKAEQVGAPVRLEGRDFTGERQPDGGLSYRGATWSLDGLSVALRGPHQRQNAAVAL
ACLEALQERGVTAPPEAARAGLASACWPGRLEEVGQQPILLLDGAHNPAGVEVLLASLKDLYPGRRLHLVFGVVGDKDRG
PMMRALLPECASVQLTPLETPRSLAPEAYLAEARALCADVTAWPDADAALAAARKRAAANDLVLCTGSLFLVGMMKARMY
RNLGAMHQPP
>Mature_410_residues
MERVQEALAALGHPERRYPALHVAGTNGKGSTCAMTAASLHAAGHRVGLYTSPHLVRVNERIRVDSEDISDADFGLRILE
VLERYPSAVSSPMTYFEFGTVVALWHFAQVRVDVAVLETGLGGRLDATTAASPVVTAITPVSFDHMEYLGDTLAAIAGEK
AGILKPGVPCVVARQAPEALEAILRKAEQVGAPVRLEGRDFTGERQPDGGLSYRGATWSLDGLSVALRGPHQRQNAAVAL
ACLEALQERGVTAPPEAARAGLASACWPGRLEEVGQQPILLLDGAHNPAGVEVLLASLKDLYPGRRLHLVFGVVGDKDRG
PMMRALLPECASVQLTPLETPRSLAPEAYLAEARALCADVTAWPDADAALAAARKRAAANDLVLCTGSLFLVGMMKARMY
RNLGAMHQPP

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=351, Percent_Identity=34.1880341880342, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI66932990, Length=351, Percent_Identity=34.1880341880342, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI1788654, Length=395, Percent_Identity=34.9367088607595, Blast_Score=187, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI71984923, Length=461, Percent_Identity=29.9349240780911, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17553150, Length=461, Percent_Identity=29.9349240780911, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17553148, Length=461, Percent_Identity=29.9349240780911, Blast_Score=149, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6323760, Length=350, Percent_Identity=31.4285714285714, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324815, Length=431, Percent_Identity=28.7703016241299, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6322718, Length=354, Percent_Identity=26.271186440678, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24641571, Length=426, Percent_Identity=29.81220657277, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24581568, Length=178, Percent_Identity=37.0786516853933, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 43558; Mature: 43558

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERVQEALAALGHPERRYPALHVAGTNGKGSTCAMTAASLHAAGHRVGLYTSPHLVRVNE
CHHHHHHHHHHCCCCHHCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEECC
RIRVDSEDISDADFGLRILEVLERYPSAVSSPMTYFEFGTVVALWHFAQVRVDVAVLETG
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEHHHEECC
LGGRLDATTAASPVVTAITPVSFDHMEYLGDTLAAIAGEKAGILKPGVPCVVARQAPEAL
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHH
EAILRKAEQVGAPVRLEGRDFTGERQPDGGLSYRGATWSLDGLSVALRGPHQRQNAAVAL
HHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCHHHHHHHH
ACLEALQERGVTAPPEAARAGLASACWPGRLEEVGQQPILLLDGAHNPAGVEVLLASLKD
HHHHHHHHCCCCCCCHHHHCCCHHCCCCCHHHHCCCCCEEEEECCCCCHHHHHHHHHHHH
LYPGRRLHLVFGVVGDKDRGPMMRALLPECASVQLTPLETPRSLAPEAYLAEARALCADV
HCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCEEEECCCCCHHHCCHHHHHHHHHHHHHC
TAWPDADAALAAARKRAAANDLVLCTGSLFLVGMMKARMYRNLGAMHQPP
CCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MERVQEALAALGHPERRYPALHVAGTNGKGSTCAMTAASLHAAGHRVGLYTSPHLVRVNE
CHHHHHHHHHHCCCCHHCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEECC
RIRVDSEDISDADFGLRILEVLERYPSAVSSPMTYFEFGTVVALWHFAQVRVDVAVLETG
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEHHHEECC
LGGRLDATTAASPVVTAITPVSFDHMEYLGDTLAAIAGEKAGILKPGVPCVVARQAPEAL
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHH
EAILRKAEQVGAPVRLEGRDFTGERQPDGGLSYRGATWSLDGLSVALRGPHQRQNAAVAL
HHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCHHHHHHHH
ACLEALQERGVTAPPEAARAGLASACWPGRLEEVGQQPILLLDGAHNPAGVEVLLASLKD
HHHHHHHHCCCCCCCHHHHCCCHHCCCCCHHHHCCCCCEEEEECCCCCHHHHHHHHHHHH
LYPGRRLHLVFGVVGDKDRGPMMRALLPECASVQLTPLETPRSLAPEAYLAEARALCADV
HCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCEEEECCCCCHHHCCHHHHHHHHHHHHHC
TAWPDADAALAAARKRAAANDLVLCTGSLFLVGMMKARMYRNLGAMHQPP
CCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]