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The map label for this gene is insF1 [H]

Identifier: 108758829

GI number: 108758829

Start: 2091547

End: 2092074

Strand: Reverse

Name: insF1 [H]

Synonym: MXAN_1764

Alternate gene names: 108758829

Gene position: 2092074-2091547 (Counterclockwise)

Preceding gene: 108761993

Following gene: 108759614

Centisome position: 22.89

GC content: 59.66

Gene sequence:

>528_bases
GTGACCCGCACAGTCCATGGCAGCGAGGCACGAACGAGCACACCAACGGACTGCTCCGTCAGTACTTCCCGAAAGGGACG
GACCTGTCCCGTTACTCTCGTGCGGAGCTGGCCACTGTCGCCCTTGCCCTCAACAGCCGGCCACGCAAGACGCTTGGCTG
GAAAACACCTGCCGAGGCTCTCAACTCTTATCTATACTTACTTCAACCAAACGGCGTTGCGACGACCGCTTGAATCCGAG
CAATACGCCAGCGAGGACTACCGGGAGCGGCTGGCAGAGCACGGAGTCACCTGCTCGATGAGCCGCAAGGGCAATTGCTG
GGACAACGCCGTCGTCGAAAGCTTCTTCAGCAGCTTGAAGATGGAGCTCGTCCACACGCGCCGCTTCCTGACGCGAGAAG
AGGCTCGACGAGCGCTATTCGATTTCATTGAGGTGTTCTACAACCGGAAGCGCCGTCACTCCTCACTCGGCTACATCAGC
CCTGTGGAATACGAGCGAACGGCTGAGTTGCGAAGGCAGGCAGCGTAG

Upstream 100 bases:

>100_bases
CGCGAAGGAGATGAAGTGAAGGTCCGCTTCATCCACGTGGAGAAGGCCTCTCTGCCAGTAGCCCTCCTGCGCGCCGACAC
GGGCTTGGCCATCTACTTCT

Downstream 100 bases:

>100_bases
CCAACCTGTCCACCGAAGCGAGTGATGCTCACAGCCCTGCGCAGTGGCTCGAGCCAGCCTTGCCAGGCAGGGCTCAAGGT
CGCGGTGAAGAATTCTGCCC

Product: IS5 family transposase orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MTRTVHGSEARTSTPTDCSVSTSRKGRTCPVTLVRSWPLSPLPSTAGHARRLAGKHLPRLSTLIYTYFNQTALRRPLESE
QYASEDYRERLAEHGVTCSMSRKGNCWDNAVVESFFSSLKMELVHTRRFLTREEARRALFDFIEVFYNRKRRHSSLGYIS
PVEYERTAELRRQAA

Sequences:

>Translated_175_residues
MTRTVHGSEARTSTPTDCSVSTSRKGRTCPVTLVRSWPLSPLPSTAGHARRLAGKHLPRLSTLIYTYFNQTALRRPLESE
QYASEDYRERLAEHGVTCSMSRKGNCWDNAVVESFFSSLKMELVHTRRFLTREEARRALFDFIEVFYNRKRRHSSLGYIS
PVEYERTAELRRQAA
>Mature_174_residues
TRTVHGSEARTSTPTDCSVSTSRKGRTCPVTLVRSWPLSPLPSTAGHARRLAGKHLPRLSTLIYTYFNQTALRRPLESEQ
YASEDYRERLAEHGVTCSMSRKGNCWDNAVVESFFSSLKMELVHTRRFLTREEARRALFDFIEVFYNRKRRHSSLGYISP
VEYERTAELRRQAA

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=85, Percent_Identity=51.7647058823529, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1787263, Length=85, Percent_Identity=51.7647058823529, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1786753, Length=85, Percent_Identity=51.7647058823529, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1786570, Length=85, Percent_Identity=51.7647058823529, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1786490, Length=85, Percent_Identity=51.7647058823529, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1789981, Length=84, Percent_Identity=45.2380952380952, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 20204; Mature: 20073

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTVHGSEARTSTPTDCSVSTSRKGRTCPVTLVRSWPLSPLPSTAGHARRLAGKHLPRL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCHHH
STLIYTYFNQTALRRPLESEQYASEDYRERLAEHGVTCSMSRKGNCWDNAVVESFFSSLK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHH
MELVHTRRFLTREEARRALFDFIEVFYNRKRRHSSLGYISPVEYERTAELRRQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TRTVHGSEARTSTPTDCSVSTSRKGRTCPVTLVRSWPLSPLPSTAGHARRLAGKHLPRL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCHHH
STLIYTYFNQTALRRPLESEQYASEDYRERLAEHGVTCSMSRKGNCWDNAVVESFFSSLK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHH
MELVHTRRFLTREEARRALFDFIEVFYNRKRRHSSLGYISPVEYERTAELRRQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]