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The map label for this gene is purA

Identifier: 108758635

GI number: 108758635

Start: 3057198

End: 3058508

Strand: Direct

Name: purA

Synonym: MXAN_2618

Alternate gene names: 108758635

Gene position: 3057198-3058508 (Clockwise)

Preceding gene: 108763229

Following gene: 108761354

Centisome position: 33.45

GC content: 69.26

Gene sequence:

>1311_bases
ATGCCGAACGTCGTCGTCATCGGAGCGCAGTGGGGAGATGAGGGGAAGGGCAAGGTCGTTGACCTGCTCACCGAGCACGC
CCAGGTCGTCGTCCGCTTCCAGGGCGGCAACAACGCGGGCCACACGCTGGTGGTGGGTGGGCAGAAGACAGTCCTCCACC
TGATTCCCTCGGGTATCCTCCACCCTGGGAAGACGTGTGTGATTGGCAACGGAGTGGTGGTGGATCCTGCCGTCCTCGTC
GGGGAGATTGACGCGCTGAAGGTGCGCGGCTTCCTCAAGGACGACGCGCAGCTCCTCATCTCCGACAATGCCCACGTCAT
CTTCCCGTGGCACAAGCTGCTGGACAGCTTCCGCGAGAAGGCGCGCGGCGGCAGCGCCATTGGCACCACGGGCCGGGGCA
TTGGCCCCGCGTACGAGGACAAGGTGGCCCGTCGCGGCATCCGCGTGCGGGACCTGCTCAACGCGGACCGGCTGCGCACG
CGCATCGAGGCCCGGCTGCCGGCGGCGCTGGACGAGCTGAAGGACCTGTGCGCGCAGGCGGGCGACCCGGTGCCGCAGCT
CGAGGTGCCGCAGATACTGGCGGAGTTCACCGGCCTGGGCGAGCGGCTCAAGCCCTTCGTCCACGACGCCTCGCTCTATC
TGTCCGGTCAGGTGCGCCGCGGCGCCCGCATCCTCTTCGAGGGCGCGCAGGGCACGCTGCTGGACGTGGACCACGGCACC
TATCCCTTCGTGACGTCCTCCAACTGCGTGGCGGGCAACGCGGCGGTGGGCTCGGGCCTGGGGCCCACGGCCATCGACAA
GGTGATGGGCATCAGCAAGGCCTACACCACGCGCGTGGGCGGCGGTCCGTTCCCCACGGAGCTGCACGACGCCATCGGCG
ACCAGTTGCGCAAGATGGGCGACGAGTTCGGCACGACCACGGGTCGGCCCCGTCGCTGCGGGTGGCTGGACGGCGTGGTG
CTGCGCTACGCCTCGCGCGTCAATGGCCTGTGGGGCATGGCCCTCACCAAGCTGGACGTGCTCAGCGGCCTCAAGACGCT
GCAGATCTGCACCGCGTACGAGCTGGACGGCCAGAAGGTGATGGAGCTGCCCGGCGACTACGAGGACCTGGCGCGCGTCA
AGCCCATCTACGAGACGCTCCAGGGCTGGGACGAGCAGATCGCCGGCGTGCGGACCTTCGACGAGCTGCCGGAGAACGCC
AAGCGCTACGTGCGCCGGGTGGAGGAGGTCAGCGGCGTCCCCGTGGTGTGCGTCTCCGTGGGCGCTGACCGCGGTGAGAC
GGTGCTGCTCCAGAACCCGTTCCGGAGCTGA

Upstream 100 bases:

>100_bases
TGAATGGGATGGGAGGGAGAAGGCAGCCTGCCAAGCCCTGCATTGTGGCTTCGCGGAACACTGTCACCCGGCCCCCCGCG
CGTGCTAAGCGGCGAGCACC

Downstream 100 bases:

>100_bases
GCGGAGGCGAAGGGCTGGCGTCGCTCGCGGGCGTGCTGACGCGGCCCTGGTTTTTCTGCTACGGCTTCCGGATCGATACC
CCGGGAGCCGTCATGGTCGT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MPNVVVIGAQWGDEGKGKVVDLLTEHAQVVVRFQGGNNAGHTLVVGGQKTVLHLIPSGILHPGKTCVIGNGVVVDPAVLV
GEIDALKVRGFLKDDAQLLISDNAHVIFPWHKLLDSFREKARGGSAIGTTGRGIGPAYEDKVARRGIRVRDLLNADRLRT
RIEARLPAALDELKDLCAQAGDPVPQLEVPQILAEFTGLGERLKPFVHDASLYLSGQVRRGARILFEGAQGTLLDVDHGT
YPFVTSSNCVAGNAAVGSGLGPTAIDKVMGISKAYTTRVGGGPFPTELHDAIGDQLRKMGDEFGTTTGRPRRCGWLDGVV
LRYASRVNGLWGMALTKLDVLSGLKTLQICTAYELDGQKVMELPGDYEDLARVKPIYETLQGWDEQIAGVRTFDELPENA
KRYVRRVEEVSGVPVVCVSVGADRGETVLLQNPFRS

Sequences:

>Translated_436_residues
MPNVVVIGAQWGDEGKGKVVDLLTEHAQVVVRFQGGNNAGHTLVVGGQKTVLHLIPSGILHPGKTCVIGNGVVVDPAVLV
GEIDALKVRGFLKDDAQLLISDNAHVIFPWHKLLDSFREKARGGSAIGTTGRGIGPAYEDKVARRGIRVRDLLNADRLRT
RIEARLPAALDELKDLCAQAGDPVPQLEVPQILAEFTGLGERLKPFVHDASLYLSGQVRRGARILFEGAQGTLLDVDHGT
YPFVTSSNCVAGNAAVGSGLGPTAIDKVMGISKAYTTRVGGGPFPTELHDAIGDQLRKMGDEFGTTTGRPRRCGWLDGVV
LRYASRVNGLWGMALTKLDVLSGLKTLQICTAYELDGQKVMELPGDYEDLARVKPIYETLQGWDEQIAGVRTFDELPENA
KRYVRRVEEVSGVPVVCVSVGADRGETVLLQNPFRS
>Mature_435_residues
PNVVVIGAQWGDEGKGKVVDLLTEHAQVVVRFQGGNNAGHTLVVGGQKTVLHLIPSGILHPGKTCVIGNGVVVDPAVLVG
EIDALKVRGFLKDDAQLLISDNAHVIFPWHKLLDSFREKARGGSAIGTTGRGIGPAYEDKVARRGIRVRDLLNADRLRTR
IEARLPAALDELKDLCAQAGDPVPQLEVPQILAEFTGLGERLKPFVHDASLYLSGQVRRGARILFEGAQGTLLDVDHGTY
PFVTSSNCVAGNAAVGSGLGPTAIDKVMGISKAYTTRVGGGPFPTELHDAIGDQLRKMGDEFGTTTGRPRRCGWLDGVVL
RYASRVNGLWGMALTKLDVLSGLKTLQICTAYELDGQKVMELPGDYEDLARVKPIYETLQGWDEQIAGVRTFDELPENAK
RYVRRVEEVSGVPVVCVSVGADRGETVLLQNPFRS

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=434, Percent_Identity=44.4700460829493, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI22748717, Length=435, Percent_Identity=44.8275862068966, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI40316944, Length=405, Percent_Identity=42.962962962963, Blast_Score=306, Evalue=3e-83,
Organism=Escherichia coli, GI1790620, Length=433, Percent_Identity=52.6558891454965, Blast_Score=437, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI25146553, Length=437, Percent_Identity=41.8764302059497, Blast_Score=334, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI25146555, Length=437, Percent_Identity=41.8764302059497, Blast_Score=334, Evalue=6e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=434, Percent_Identity=42.1658986175115, Blast_Score=355, Evalue=9e-99,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=44.4444444444444, Blast_Score=349, Evalue=2e-96,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_MYXXD (Q1D936)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_630837.1
- ProteinModelPortal:   Q1D936
- SMR:   Q1D936
- STRING:   Q1D936
- GeneID:   4104508
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_2618
- TIGR:   MXAN_2618
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   Q1D936
- ProtClustDB:   PRK01117
- BioCyc:   MXAN246197:MXAN_2618-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46980; Mature: 46849

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 130-130 BINDING 144-144 BINDING 230-230 BINDING 245-245 BINDING 309-309 BINDING 311-311

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNVVVIGAQWGDEGKGKVVDLLTEHAQVVVRFQGGNNAGHTLVVGGQKTVLHLIPSGIL
CCCEEEEECCCCCCCCCHHHHHHHHCEEEEEEECCCCCCCCEEEECCCHHEEECCCCCCC
HPGKTCVIGNGVVVDPAVLVGEIDALKVRGFLKDDAQLLISDNAHVIFPWHKLLDSFREK
CCCCEEEECCCEEECCHHHHCCCHHHEEEEECCCCCEEEEECCCEEEECHHHHHHHHHHH
ARGGSAIGTTGRGIGPAYEDKVARRGIRVRDLLNADRLRTRIEARLPAALDELKDLCAQA
HCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHCHHHHHHHHHHHCHHHHHHHHHHHHHC
GDPVPQLEVPQILAEFTGLGERLKPFVHDASLYLSGQVRRGARILFEGAQGTLLDVDHGT
CCCCCCCHHHHHHHHHHCCCHHHHHHHHCCEEEEECCCCCCCEEEEECCCCCEEECCCCC
YPFVTSSNCVAGNAAVGSGLGPTAIDKVMGISKAYTTRVGGGPFPTELHDAIGDQLRKMG
CCEEECCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
DEFGTTTGRPRRCGWLDGVVLRYASRVNGLWGMALTKLDVLSGLKTLQICTAYELDGQKV
HHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHEEEEECCCCHH
MELPGDYEDLARVKPIYETLQGWDEQIAGVRTFDELPENAKRYVRRVEEVSGVPVVCVSV
CCCCCCHHHHHHHHHHHHHHHCHHHHHCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEEEE
GADRGETVLLQNPFRS
CCCCCCEEEECCCCCC
>Mature Secondary Structure 
PNVVVIGAQWGDEGKGKVVDLLTEHAQVVVRFQGGNNAGHTLVVGGQKTVLHLIPSGIL
CCEEEEECCCCCCCCCHHHHHHHHCEEEEEEECCCCCCCCEEEECCCHHEEECCCCCCC
HPGKTCVIGNGVVVDPAVLVGEIDALKVRGFLKDDAQLLISDNAHVIFPWHKLLDSFREK
CCCCEEEECCCEEECCHHHHCCCHHHEEEEECCCCCEEEEECCCEEEECHHHHHHHHHHH
ARGGSAIGTTGRGIGPAYEDKVARRGIRVRDLLNADRLRTRIEARLPAALDELKDLCAQA
HCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHCHHHHHHHHHHHCHHHHHHHHHHHHHC
GDPVPQLEVPQILAEFTGLGERLKPFVHDASLYLSGQVRRGARILFEGAQGTLLDVDHGT
CCCCCCCHHHHHHHHHHCCCHHHHHHHHCCEEEEECCCCCCCEEEEECCCCCEEECCCCC
YPFVTSSNCVAGNAAVGSGLGPTAIDKVMGISKAYTTRVGGGPFPTELHDAIGDQLRKMG
CCEEECCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
DEFGTTTGRPRRCGWLDGVVLRYASRVNGLWGMALTKLDVLSGLKTLQICTAYELDGQKV
HHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHEEEEECCCCHH
MELPGDYEDLARVKPIYETLQGWDEQIAGVRTFDELPENAKRYVRRVEEVSGVPVVCVSV
CCCCCCHHHHHHHHHHHHHHHCHHHHHCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEEEE
GADRGETVLLQNPFRS
CCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA