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The map label for this gene is purB [H]

Identifier: 108758602

GI number: 108758602

Start: 2413025

End: 2414338

Strand: Direct

Name: purB [H]

Synonym: MXAN_2079

Alternate gene names: 108758602

Gene position: 2413025-2414338 (Clockwise)

Preceding gene: 108762824

Following gene: 108759067

Centisome position: 26.4

GC content: 67.88

Gene sequence:

>1314_bases
GTGATTCCGCGATACAGCCGTCAGGAGATGTCCTCCCTCTGGTCCGACGAGGCCCGTTACAGCCGTTGGCGCGACGTGGA
ACTCGCCGCCCTGGAGGGCATGGTGGAGGCCGGGCTGGCGCCCCGCGAGGCGCTGCAGGACTGCCTGGCTCGCGCCGGAG
ACTTCACGCCCGGGGACGCGGCGAAAATCGAGGAGATTGAGCGCACCACCAAGCACGACGTCATCGCTTTCCTCACCTTC
ATGGAAGAGCGGGTGGGGCCCAGCGCGCGCTGGCTGCACCTGGGCATGACGTCGTCGGACGTACTGGACACGTCGCTGGC
GCTCACGCTGCGTGACGCGCTGGACCTCATCCTGAAGGATGTGGACCGTGTCATGGCCGCGGTGGAGAAGCGCGCCTTCG
AGCACAAGCACACGCTGCAGATGGGCCGCAGCCACGGCATCCACGCGGAGCCCATCACCTTCGGCCACAAGCTGGCCATC
TGGTACGACGAGCTGCGCCGTGCCCGGACGCGCCTGGAGCACGCGCGGGACGTCATCGCCGTGGGGAAGATTTCCGGCGC
GGTGGGCACCTTCGCGCACCTGCCGCCCGCGGTGGAGGAGCACGTCTGCCGCAAGCTGGGCCTCAAGCCCGCGCCGGCCT
CCAGCCAGGTGGTGCAGCGAGACAGGCACGCGGAGTTCTTCACCGCGCTGGCGCTGCTGGGCTCGAGCATCGAGAAGTTC
GCCGTGGAGATTCGCCACCTCCAGCGTACCGAGGTGCGTGAGGCGGAGGAGCCCTTCACCGCGGGACAGAAGGGCTCCAG
CGCCATGCCGCACAAGCGCAACCCGATTCTGTCGGAGAACCTCACCGGCCTGGCGCGCCTGCTGCGCGGCTACGCGGTGA
GCGCCATGGAGGACGTGGCGCTGTGGCATGAGCGGGACATCTCCCACTCCTCGGTGGAGCGTGTCATCGGCCCGGACGCC
ACCATCCTCGCGGACTTCATGCTCATCCGCTTCGCGCGGCTGATGGAGGACCTGCGCGTCTACCCGGAGCAGATGAAGAA
GAACCTGGACCTGCTGGGCGGCGTGGTGAACTCGCAGCGCCTCCTGTTGGAACTGGCGCGCAAGGGCATGGACCGGCAGG
CCGCGTACGTCATCGTCCAGCGCAACGCGATGAAGCTGTACGAGGAAGGACTCGACTTCCGGCAGGCCCTGCTCGCGGAC
GAGGACCTGCGGAAGATGATGACGCCCGAGGAGATTTCCGACTGCTTCTCCTCGGGCTACCACACGCGCCACATGGACGA
CGTCTTCCAGCGCGTGTTCGGCCGGAGCGCGTAG

Upstream 100 bases:

>100_bases
TCAATCTCACCGCCGCCAGGAAGTTGGGTCCGTCCTCCGACGCCGTGCTGGAACTCCTCCGGCTCGCGGCGCGGCGGGAC
TTTGCCGTGAAGGTCTACGA

Downstream 100 bases:

>100_bases
GCACCCTGTGCCGATGATGGAGGAGGGCTACTTCAGGTAGCCCTTCTCACGCAGGTTGCGCTCGATGCGGGTCTTCACGT
CGCCCGGCTCCAGCGTCAGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MIPRYSRQEMSSLWSDEARYSRWRDVELAALEGMVEAGLAPREALQDCLARAGDFTPGDAAKIEEIERTTKHDVIAFLTF
MEERVGPSARWLHLGMTSSDVLDTSLALTLRDALDLILKDVDRVMAAVEKRAFEHKHTLQMGRSHGIHAEPITFGHKLAI
WYDELRRARTRLEHARDVIAVGKISGAVGTFAHLPPAVEEHVCRKLGLKPAPASSQVVQRDRHAEFFTALALLGSSIEKF
AVEIRHLQRTEVREAEEPFTAGQKGSSAMPHKRNPILSENLTGLARLLRGYAVSAMEDVALWHERDISHSSVERVIGPDA
TILADFMLIRFARLMEDLRVYPEQMKKNLDLLGGVVNSQRLLLELARKGMDRQAAYVIVQRNAMKLYEEGLDFRQALLAD
EDLRKMMTPEEISDCFSSGYHTRHMDDVFQRVFGRSA

Sequences:

>Translated_437_residues
MIPRYSRQEMSSLWSDEARYSRWRDVELAALEGMVEAGLAPREALQDCLARAGDFTPGDAAKIEEIERTTKHDVIAFLTF
MEERVGPSARWLHLGMTSSDVLDTSLALTLRDALDLILKDVDRVMAAVEKRAFEHKHTLQMGRSHGIHAEPITFGHKLAI
WYDELRRARTRLEHARDVIAVGKISGAVGTFAHLPPAVEEHVCRKLGLKPAPASSQVVQRDRHAEFFTALALLGSSIEKF
AVEIRHLQRTEVREAEEPFTAGQKGSSAMPHKRNPILSENLTGLARLLRGYAVSAMEDVALWHERDISHSSVERVIGPDA
TILADFMLIRFARLMEDLRVYPEQMKKNLDLLGGVVNSQRLLLELARKGMDRQAAYVIVQRNAMKLYEEGLDFRQALLAD
EDLRKMMTPEEISDCFSSGYHTRHMDDVFQRVFGRSA
>Mature_437_residues
MIPRYSRQEMSSLWSDEARYSRWRDVELAALEGMVEAGLAPREALQDCLARAGDFTPGDAAKIEEIERTTKHDVIAFLTF
MEERVGPSARWLHLGMTSSDVLDTSLALTLRDALDLILKDVDRVMAAVEKRAFEHKHTLQMGRSHGIHAEPITFGHKLAI
WYDELRRARTRLEHARDVIAVGKISGAVGTFAHLPPAVEEHVCRKLGLKPAPASSQVVQRDRHAEFFTALALLGSSIEKF
AVEIRHLQRTEVREAEEPFTAGQKGSSAMPHKRNPILSENLTGLARLLRGYAVSAMEDVALWHERDISHSSVERVIGPDA
TILADFMLIRFARLMEDLRVYPEQMKKNLDLLGGVVNSQRLLLELARKGMDRQAAYVIVQRNAMKLYEEGLDFRQALLAD
EDLRKMMTPEEISDCFSSGYHTRHMDDVFQRVFGRSA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=406, Percent_Identity=28.8177339901478, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=28.9817232375979, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1787376, Length=320, Percent_Identity=30, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17508577, Length=414, Percent_Identity=28.5024154589372, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32564234, Length=341, Percent_Identity=30.2052785923754, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323391, Length=405, Percent_Identity=28.641975308642, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24647570, Length=405, Percent_Identity=29.1358024691358, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49392; Mature: 49392

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRQEMSSLWSDEARYSRWRDVELAALEGMVEAGLAPREALQDCLARAGDFTPGDA
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCH
AKIEEIERTTKHDVIAFLTFMEERVGPSARWLHLGMTSSDVLDTSLALTLRDALDLILKD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
VDRVMAAVEKRAFEHKHTLQMGRSHGIHAEPITFGHKLAIWYDELRRARTRLEHARDVIA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VGKISGAVGTFAHLPPAVEEHVCRKLGLKPAPASSQVVQRDRHAEFFTALALLGSSIEKF
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AVEIRHLQRTEVREAEEPFTAGQKGSSAMPHKRNPILSENLTGLARLLRGYAVSAMEDVA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LWHERDISHSSVERVIGPDATILADFMLIRFARLMEDLRVYPEQMKKNLDLLGGVVNSQR
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LLLELARKGMDRQAAYVIVQRNAMKLYEEGLDFRQALLADEDLRKMMTPEEISDCFSSGY
HHHHHHHCCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
HTRHMDDVFQRVFGRSA
CCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIPRYSRQEMSSLWSDEARYSRWRDVELAALEGMVEAGLAPREALQDCLARAGDFTPGDA
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCH
AKIEEIERTTKHDVIAFLTFMEERVGPSARWLHLGMTSSDVLDTSLALTLRDALDLILKD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
VDRVMAAVEKRAFEHKHTLQMGRSHGIHAEPITFGHKLAIWYDELRRARTRLEHARDVIA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VGKISGAVGTFAHLPPAVEEHVCRKLGLKPAPASSQVVQRDRHAEFFTALALLGSSIEKF
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AVEIRHLQRTEVREAEEPFTAGQKGSSAMPHKRNPILSENLTGLARLLRGYAVSAMEDVA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LWHERDISHSSVERVIGPDATILADFMLIRFARLMEDLRVYPEQMKKNLDLLGGVVNSQR
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LLLELARKGMDRQAAYVIVQRNAMKLYEEGLDFRQALLADEDLRKMMTPEEISDCFSSGY
HHHHHHHCCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
HTRHMDDVFQRVFGRSA
CCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]