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The map label for this gene is trmL [H]

Identifier: 108758130

GI number: 108758130

Start: 2374808

End: 2375311

Strand: Direct

Name: trmL [H]

Synonym: MXAN_2046

Alternate gene names: 108758130

Gene position: 2374808-2375311 (Clockwise)

Preceding gene: 108762954

Following gene: 108760730

Centisome position: 25.98

GC content: 67.86

Gene sequence:

>504_bases
ATGCTCGAGCCCCTGGCGCGTCCTCTCCACCTGGTCCTCGTTTCTCCCCAGATTCCCCCCAACACCGGCAACGTCGCCCG
CCTGTGCGCCGTGACGGGCTGTCGGCTCATCCTGGTGGAGCCCCTGGGCTTCTCCATTGACGACCGGCAGCTCAAGCGGG
CGGGGCTGGACTACTGGGACAAGGTATTTCTCCGCCTGTATCCAACCTACGCGGCCTATGCGGCGGACTACCCGGACGCC
CGGCGCTGGCTCTTCTCCGCCCGGGCTGAAACATCCCTGTATGAGGCCCGGTTCGAGGAGGGGGACCACCTGGTGTTCGG
CTCGGAGGTGTCCGGGCTGGCGCCGGAGGTGATGGAGGGGGGCACGGGGACGGCCGTCACCATTCCCATGCTGGAGGACC
GCCGGAGCCTGAACCTGTCCACGTCGGTGGGAATCGGGACCTATGAGGCCCTGCGACAGGTCCGTTTCACCGGAGCGGGC
AGGCAAGCGCCCCCGGCAAGTTGA

Upstream 100 bases:

>100_bases
AACCGTTGGAGGAACGAGGTGGCCTTGTCGGCCCGGTCCGCCAGCAGCCGTTGCCGCGTCTCGTTGTTCACCTTCCAGCG
CATGTAGCGCCTTCTACCCC

Downstream 100 bases:

>100_bases
GGGGCCGTGTAGAGGGACTACACTGCGGCGACGCATGACCCCGTCGCAGGCCGCCGAAGCGCTCTATTCCGCCCACAAGT
CCCGCGCCACCGGGAGGCTC

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MLEPLARPLHLVLVSPQIPPNTGNVARLCAVTGCRLILVEPLGFSIDDRQLKRAGLDYWDKVFLRLYPTYAAYAADYPDA
RRWLFSARAETSLYEARFEEGDHLVFGSEVSGLAPEVMEGGTGTAVTIPMLEDRRSLNLSTSVGIGTYEALRQVRFTGAG
RQAPPAS

Sequences:

>Translated_167_residues
MLEPLARPLHLVLVSPQIPPNTGNVARLCAVTGCRLILVEPLGFSIDDRQLKRAGLDYWDKVFLRLYPTYAAYAADYPDA
RRWLFSARAETSLYEARFEEGDHLVFGSEVSGLAPEVMEGGTGTAVTIPMLEDRRSLNLSTSVGIGTYEALRQVRFTGAG
RQAPPAS
>Mature_167_residues
MLEPLARPLHLVLVSPQIPPNTGNVARLCAVTGCRLILVEPLGFSIDDRQLKRAGLDYWDKVFLRLYPTYAAYAADYPDA
RRWLFSARAETSLYEARFEEGDHLVFGSEVSGLAPEVMEGGTGTAVTIPMLEDRRSLNLSTSVGIGTYEALRQVRFTGAG
RQAPPAS

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=152, Percent_Identity=39.4736842105263, Blast_Score=121, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18342; Mature: 18342

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEPLARPLHLVLVSPQIPPNTGNVARLCAVTGCRLILVEPLGFSIDDRQLKRAGLDYWD
CCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHCCCCHHH
KVFLRLYPTYAAYAADYPDARRWLFSARAETSLYEARFEEGDHLVFGSEVSGLAPEVMEG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHCC
GTGTAVTIPMLEDRRSLNLSTSVGIGTYEALRQVRFTGAGRQAPPAS
CCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLEPLARPLHLVLVSPQIPPNTGNVARLCAVTGCRLILVEPLGFSIDDRQLKRAGLDYWD
CCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHCCCCHHH
KVFLRLYPTYAAYAADYPDARRWLFSARAETSLYEARFEEGDHLVFGSEVSGLAPEVMEG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHCC
GTGTAVTIPMLEDRRSLNLSTSVGIGTYEALRQVRFTGAGRQAPPAS
CCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA