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The map label for this gene is rluB [H]

Identifier: 108758119

GI number: 108758119

Start: 1861047

End: 1861847

Strand: Direct

Name: rluB [H]

Synonym: MXAN_1586

Alternate gene names: 108758119

Gene position: 1861047-1861847 (Clockwise)

Preceding gene: 108759986

Following gene: 108762355

Centisome position: 20.36

GC content: 72.03

Gene sequence:

>801_bases
ATGGCGCGGAAGCAGCGGACCCCGAGGTGGCTGGAAGCGGCACGCCGGAGGGGGCCGGAGGACGTGCAACCCTCCGGAAG
GTCGGACTGGTTGGCGAGGGCCCTGGGGAAGGCCGGAGTGATGCCTCGCGCCCAGGCGGAAGCGGCCATCCGTGAGGGAC
GGGTGACGCTCGATGGGCGGGTGGAGCACGAGCCCTTCGCGCCGGTGCGTCCGGAGATGGAAGTGCGGGTGGACGGGCTG
GTGCGGAACCTGGAGGCGCCCGTGTTGACGCTGATGTTCCACAAGCCAGCGGGGCTGGTGGTGCACGGCTCGGACGCGGA
GGGCGTGGGCACCGTGTTCGAGTGTCTGCGTGAGCGGCTCACCCAACCGCTGCGCGACTATGAGTGGCTCGCGGTGGGCC
GTCTGGACCGGGACACCACGGGGTTGCTGCTCTTCACCAACGACGAACGTCTGGTCCAACACGGGACGTCGCCCGAGCGG
CACCTGCCCAAGCGCTACGTGGCGGGCGTGGTGGGGCCGCCACCGGAGGATGCGCTCCGCCGGCTGCGTGAGGGCCTGGT
GCTGGAGGACGGCCCGACGCGGCCCGCGGGTGCCCGGCTGCGCGCGCCGGACGTGGTGGAGCTCACCCTGACGGAAGGGC
GCAACCACCAGGTGAAGCGGATGCTGGCGGCGGTGGGACATCCGGTGCGGACCCTGCACCGCGAGGCCGTGGGCGGCGTG
ACGCTGGACGTGCCCGAGGGCGCGTGGCGGTTGCTGACGGACGAGGAAGTGTCCCAGGGGTTGTCCTTCTCCGTCTCCTG
A

Upstream 100 bases:

>100_bases
ACATGCTGCATGGCTGGCCTGGACGGAGCCTGGTACGCAGGCAGGGTGGGCGGTGGAGGGCGCGGGCGAAGGTGGGTGGA
ATGAGAGAGGACGTGGGTGG

Downstream 100 bases:

>100_bases
GGCCGCACCCGCCGCGTGTAGACTCCGGGGCCATGGACGATTCCGTGGCCCGTGCGCGTGGCGGCTTCCCCGCACGGGCC
TTCTGGCTCGGTGTCTTCGG

Product: RsuA family pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MARKQRTPRWLEAARRRGPEDVQPSGRSDWLARALGKAGVMPRAQAEAAIREGRVTLDGRVEHEPFAPVRPEMEVRVDGL
VRNLEAPVLTLMFHKPAGLVVHGSDAEGVGTVFECLRERLTQPLRDYEWLAVGRLDRDTTGLLLFTNDERLVQHGTSPER
HLPKRYVAGVVGPPPEDALRRLREGLVLEDGPTRPAGARLRAPDVVELTLTEGRNHQVKRMLAAVGHPVRTLHREAVGGV
TLDVPEGAWRLLTDEEVSQGLSFSVS

Sequences:

>Translated_266_residues
MARKQRTPRWLEAARRRGPEDVQPSGRSDWLARALGKAGVMPRAQAEAAIREGRVTLDGRVEHEPFAPVRPEMEVRVDGL
VRNLEAPVLTLMFHKPAGLVVHGSDAEGVGTVFECLRERLTQPLRDYEWLAVGRLDRDTTGLLLFTNDERLVQHGTSPER
HLPKRYVAGVVGPPPEDALRRLREGLVLEDGPTRPAGARLRAPDVVELTLTEGRNHQVKRMLAAVGHPVRTLHREAVGGV
TLDVPEGAWRLLTDEEVSQGLSFSVS
>Mature_265_residues
ARKQRTPRWLEAARRRGPEDVQPSGRSDWLARALGKAGVMPRAQAEAAIREGRVTLDGRVEHEPFAPVRPEMEVRVDGLV
RNLEAPVLTLMFHKPAGLVVHGSDAEGVGTVFECLRERLTQPLRDYEWLAVGRLDRDTTGLLLFTNDERLVQHGTSPERH
LPKRYVAGVVGPPPEDALRRLREGLVLEDGPTRPAGARLRAPDVVELTLTEGRNHQVKRMLAAVGHPVRTLHREAVGGVT
LDVPEGAWRLLTDEEVSQGLSFSVS

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=233, Percent_Identity=38.1974248927039, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787524, Length=233, Percent_Identity=35.1931330472103, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI87081838, Length=188, Percent_Identity=38.2978723404255, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1790453, Length=230, Percent_Identity=30.4347826086957, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 29479; Mature: 29348

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKQRTPRWLEAARRRGPEDVQPSGRSDWLARALGKAGVMPRAQAEAAIREGRVTLDGR
CCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCC
VEHEPFAPVRPEMEVRVDGLVRNLEAPVLTLMFHKPAGLVVHGSDAEGVGTVFECLRERL
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHH
TQPLRDYEWLAVGRLDRDTTGLLLFTNDERLVQHGTSPERHLPKRYVAGVVGPPPEDALR
HHHHHCCCEEEECCCCCCCCEEEEEECCHHHHHCCCCCHHHCCHHHHCCCCCCCCHHHHH
RLREGLVLEDGPTRPAGARLRAPDVVELTLTEGRNHQVKRMLAAVGHPVRTLHREAVGGV
HHHCCCEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCE
TLDVPEGAWRLLTDEEVSQGLSFSVS
EEECCCCCEEEECHHHHHCCCCCCCC
>Mature Secondary Structure 
ARKQRTPRWLEAARRRGPEDVQPSGRSDWLARALGKAGVMPRAQAEAAIREGRVTLDGR
CCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCC
VEHEPFAPVRPEMEVRVDGLVRNLEAPVLTLMFHKPAGLVVHGSDAEGVGTVFECLRERL
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHH
TQPLRDYEWLAVGRLDRDTTGLLLFTNDERLVQHGTSPERHLPKRYVAGVVGPPPEDALR
HHHHHCCCEEEECCCCCCCCEEEEEECCHHHHHCCCCCHHHCCHHHHCCCCCCCCHHHHH
RLREGLVLEDGPTRPAGARLRAPDVVELTLTEGRNHQVKRMLAAVGHPVRTLHREAVGGV
HHHCCCEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCE
TLDVPEGAWRLLTDEEVSQGLSFSVS
EEECCCCCEEEECHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]